Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,902,302 C→A 70.5% D242Y (GAT→TAT)  pyrG ← CTP synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,902,3020CA70.5% 243.1 / 216.6 224D242Y (GAT→TAT) pyrGCTP synthetase
Reads supporting (aligned to +/- strand):  ref base C (25/41);  new base A (69/89);  total (94/130)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 225 total aligned reads displayed.

GACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTG  >  CP009273/2902156‑2902449
                                                                                                                                                  |                                                                                                                                                   
gACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCg                                                                                                                                                    <  2:855421/148‑1 (MQ=255)
      aCTTACCGGGTTCGCTTCGTCGTATATAACCTGTTCCAATTCTGACAGATTCGCTTCCGTGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCt                                                                                                                                              <  2:1836353/148‑1 (MQ=255)
         tACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGACTGTTATAGATGGAATAGACGTCTTTc                                                                                                                                           >  1:3016650/1‑148 (MQ=255)
         tACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGACTAGACGTCTTTc                                                                                                                                           >  2:2014696/1‑148 (MQ=255)
         tACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTc                                                                                                                                           >  2:2014858/1‑148 (MQ=255)
           cctggTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACATATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCag                                                                                                                                         <  2:1173341/145‑1 (MQ=255)
              ggTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACCGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAgaga                                                                                                                                      >  1:1776587/1‑148 (MQ=255)
              ggTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAgaga                                                                                                                                      >  2:1656264/1‑148 (MQ=255)
               gTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGaa                                                                                                                                     <  1:1600917/148‑1 (MQ=255)
                   gCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATaa                                                                                                                                 <  1:2920887/148‑1 (MQ=255)
                    cttcttCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAAc                                                                                                                                >  1:2263187/1‑148 (MQ=255)
                     ttcttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAAcc                                                                                                                               >  1:446884/1‑148 (MQ=255)
                     atagtcGAACATAACCTGATCCCATTCGGACAGAATCGCTACAAGGCAGTTTAATCTGAATCGTTTACAAATATAATCGTAAAGCCCGAGAGATTACAACAGGCCCGGAATTATATTGAAGTTATAGTATTCTTTCATAGAACTaaga                                                                                                                               >  2:3054001/5‑146 (MQ=255)
                      tcttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCg                                                                                                                              >  1:987229/1‑148 (MQ=255)
                      tcttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCg                                                                                                                              >  2:1242624/1‑148 (MQ=255)
                       cttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGc                                                                                                                             <  2:2177159/148‑1 (MQ=255)
                       cttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGc                                                                                                                             <  1:1762253/148‑1 (MQ=255)
                            aaGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGATTTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCttttt                                                                                                                        <  2:2755192/148‑1 (MQ=255)
                            aaGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCttttt                                                                                                                        <  2:2962917/148‑1 (MQ=255)
                            aaGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCttttt                                                                                                                        <  2:2962946/148‑1 (MQ=255)
                              gATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTcc                                                                                                                      <  2:1126095/148‑1 (MQ=255)
                              gATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTcc                                                                                                                      >  1:2768091/1‑148 (MQ=255)
                               aTAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCg                                                                                                                     >  1:2498259/1‑148 (MQ=255)
                                tAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCgg                                                                                                                    >  2:465489/1‑148 (MQ=255)
                                  aCCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGaa                                                                                                                  <  1:1010608/148‑1 (MQ=255)
                                  aCCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGaa                                                                                                                  >  1:2592566/1‑148 (MQ=255)
                                  aCCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGaa                                                                                                                  >  2:389572/1‑148 (MQ=255)
                                     tGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGACTTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAt                                                                                                               >  1:2023907/1‑148 (MQ=255)
                                     tGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAt                                                                                                               <  1:1186349/148‑1 (MQ=255)
                                       ttCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTa                                                                                                             >  1:419808/1‑148 (MQ=255)
                                       ttCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTa                                                                                                             >  1:1452254/1‑148 (MQ=255)
                                            aTTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGaa                                                                                                        >  1:3174119/1‑148 (MQ=255)
                                                ggACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAAt                                                                                                    <  2:918798/148‑1 (MQ=255)
                                                  aCAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGc                                                                                                  >  2:1186858/1‑148 (MQ=255)
                                                   cAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCa                                                                                                 <  1:775462/148‑1 (MQ=255)
                                                    aGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCaa                                                                                                <  2:2180571/148‑1 (MQ=255)
                                                      aTTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATc                                                                                              <  2:3147317/148‑1 (MQ=255)
                                                       ttCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCt                                                                                             <  1:208044/148‑1 (MQ=255)
                                                       ttCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCt                                                                                             <  2:3234809/148‑1 (MQ=255)
                                                       ttCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCt                                                                                             >  2:2617298/1‑148 (MQ=255)
                                                       ttCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCt                                                                                             <  2:2257132/148‑1 (MQ=255)
                                                            ttCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCa                                                                                        <  1:2570373/148‑1 (MQ=255)
                                                             tCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCAc                                                                                       >  2:3500915/1‑148 (MQ=255)
                                                             tCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCAc                                                                                       >  2:1738672/1‑148 (MQ=255)
                                                                gggaattttAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCAcgtt                                                                                    <  1:953081/142‑1 (MQ=255)
                                                                  gCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCAcgttcg                                                                                  <  2:1776859/148‑1 (MQ=255)
                                                                   cAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCAcgttcgt                                                                                 <  2:3156660/148‑1 (MQ=255)
                                                                     gTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACgttcgttc                                                                               >  1:1196101/1‑148 (MQ=255)
                                                                      tttAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGttcgttcg                                                                              >  1:1006245/1‑148 (MQ=255)
                                                                      tttAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGttcgttcg                                                                              >  1:543246/1‑148 (MQ=255)
                                                                      tttAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGttcgttcg                                                                              >  2:1985724/1‑148 (MQ=255)
                                                                      tttAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGttcgttcg                                                                              >  1:1156405/1‑148 (MQ=255)
                                                                       ttAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGc                                                                             >  1:761893/1‑148 (MQ=255)
                                                                         aaGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCg                                                                           >  1:1253274/1‑148 (MQ=255)
                                                                                 tCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTACGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTAGACGGAACAgcg                                                                   >  1:1519059/1‑148 (MQ=255)
                                                                                  cGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAgcgc                                                                  <  2:1698399/148‑1 (MQ=255)
                                                                                   gTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGcgcg                                                                 <  1:328326/148‑1 (MQ=255)
                                                                                     ttACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGAt                                                               <  1:2670070/148‑1 (MQ=255)
                                                                                     ttACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGAt                                                               >  2:2246021/1‑148 (MQ=255)
                                                                                      tACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATc                                                              <  2:1396971/148‑1 (MQ=255)
                                                                                          aaTATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGaa                                                          <  2:762176/148‑1 (MQ=255)
                                                                                          aaTATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGaa                                                          <  2:1308754/148‑1 (MQ=255)
                                                                                             ataATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGa                                                       >  2:2883824/1‑148 (MQ=255)
                                                                                             ataATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGa                                                       <  2:3426877/148‑1 (MQ=255)
                                                                                             ataATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGa                                                       >  1:555969/1‑148 (MQ=255)
                                                                                                  cGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAAt                                                  <  2:170492/148‑1 (MQ=255)
                                                                                                    tCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCa                                                <  2:1615603/148‑1 (MQ=255)
                                                                                                      cAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAgg                                              >  2:2667288/1‑148 (MQ=255)
                                                                                                       aGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGa                                             >  2:2418145/1‑148 (MQ=255)
                                                                                                       aGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGa                                             >  1:1054374/1‑148 (MQ=255)
                                                                                                          cccTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGt                                          <  1:1556569/148‑1 (MQ=255)
                                                                                                            cTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCa                                        >  2:162871/1‑148 (MQ=255)
                                                                                                            cTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCa                                        <  2:1196101/148‑1 (MQ=255)
                                                                                                             tGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAg                                       >  2:3341924/1‑148 (MQ=255)
                                                                                                             tGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAg                                       >  2:3343690/1‑148 (MQ=255)
                                                                                                              gagaTTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAgg                                      >  2:1284082/1‑148 (MQ=255)
                                                                                                              gagaTTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAgg                                      >  2:2107473/1‑148 (MQ=255)
                                                                                                              gagaTTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAgg                                      <  1:1417835/148‑1 (MQ=255)
                                                                                                                gaTTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCt                                    <  1:711805/148‑1 (MQ=255)
                                                                                                                   ttCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGa                                 <  2:2932969/148‑1 (MQ=255)
                                                                                                                     cAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATa                               <  2:1567053/148‑1 (MQ=255)
                                                                                                                        cAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCg                            <  2:1452254/148‑1 (MQ=255)
                                                                                                                         aGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGa                           <  1:57127/148‑1 (MQ=255)
                                                                                                                          ggCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGAt                          <  1:3516801/148‑1 (MQ=255)
                                                                                                                           gCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATg                         >  1:2108621/1‑148 (MQ=255)
                                                                                                                           gCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATg                         >  1:1109169/1‑148 (MQ=255)
                                                                                                                             ccGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGga                       >  2:721262/1‑148 (MQ=255)
                                                                                                                              cGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGgag                      <  2:3322460/148‑1 (MQ=255)
                                                                                                                               ggAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGgaga                     <  2:2930012/148‑1 (MQ=255)
                                                                                                                                 aaTTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGagc                   >  1:2521892/1‑148 (MQ=255)
                                                                                                                                     ttATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGct               <  2:3016650/148‑1 (MQ=255)
                                                                                                                                      tataGATGGAATAGACGTCTTTCAGATAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGTACAGCGCTATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGctc              <  1:2467734/148‑1 (MQ=255)
                                                                                                                                       ataGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGctct             <  2:2610991/148‑1 (MQ=255)
                                                                                                                                        taGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCtt            <  2:2768091/148‑1 (MQ=255)
                                                                                                                                        taGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCtt            <  1:3382113/148‑1 (MQ=255)
                                                                                                                                         aGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCttt           <  1:836545/148‑1 (MQ=255)
                                                                                                                                          gATGGAATAGACGTCTTTCAGAGAAATAACCGCTATTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTACGTTCGCCGGAACAGCGCGATCTGAACGACAAATCCGGATGTCAGGCTGGATACCGAGGGAGAGCAGCTCtttt          >  1:91157/1‑148 (MQ=255)
                                                                                                                                            tGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGAGATCTGAAAGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTAc        <  1:3488670/148‑1 (MQ=255)
                                                                                                                                              gAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACag      <  2:2498239/148‑1 (MQ=255)
                                                                                                                                                  cGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTg  <  1:2907894/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTG  >  CP009273/2902156‑2902449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑