Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 27,307 C→T 100% I5I (ATC→ATT)  rihC → ribonucleoside hydrolase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927327,3070CT99.4% 536.5 / ‑4.3 165I5I (ATC→ATT) rihCribonucleoside hydrolase 3
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (97/67);  total (98/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 170 total aligned reads displayed.

CGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGC  >  CP009273/27162‑27449
                                                                                                                                                 |                                                                                                                                              
cGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTAtttt                                                                                                                                              >  1:2686975/1‑148 (MQ=255)
cGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTAtttt                                                                                                                                              <  1:3274996/148‑1 (MQ=255)
cGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTAtttt                                                                                                                                              >  1:904327/1‑148 (MQ=255)
 gTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTc                                                                                                                                             >  1:2004650/1‑148 (MQ=255)
 gTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTc                                                                                                                                             <  1:125794/148‑1 (MQ=255)
       aaaaCATTGTTTTCGTAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGTTTTAGTACTTAGCAGCCTAAGTTATGCGAAAATGGCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGAt                                                                                                                                       <  1:536240/148‑1 (MQ=255)
        aaaCATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATa                                                                                                                                      <  1:2277687/148‑1 (MQ=255)
        aaaCATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATa                                                                                                                                      >  2:1148876/1‑148 (MQ=255)
         aaCATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATAc                                                                                                                                     >  1:2267786/1‑148 (MQ=255)
         aaCATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATAc                                                                                                                                     >  1:1879193/1‑148 (MQ=255)
          aCATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACt                                                                                                                                    >  2:1047767/1‑148 (MQ=255)
          aCATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACt                                                                                                                                    <  1:10004/148‑1 (MQ=255)
          aCATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACt                                                                                                                                    <  1:10019/148‑1 (MQ=255)
           cATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTg                                                                                                                                   >  2:482506/1‑148 (MQ=255)
           cATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGAGTTTACCTATTTTCCTCGATACTg                                                                                                                                   >  2:44204/1‑148 (MQ=255)
              tGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGAcc                                                                                                                                <  1:2844340/148‑1 (MQ=255)
              tGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGAcc                                                                                                                                <  1:2844094/148‑1 (MQ=255)
                 tttCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCg                                                                                                                             >  1:1217239/1‑148 (MQ=255)
                  ttCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGGTTACCTATTTTCCTCGATACTGACCCCgg                                                                                                                            >  2:3321863/1‑148 (MQ=255)
                    cGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCa                                                                                                                          <  2:2579512/148‑1 (MQ=255)
                    cGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCa                                                                                                                          <  2:3459832/148‑1 (MQ=255)
                    cGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGGTTACCTATTTTCCTCGATACTGACCCCGGCa                                                                                                                          >  2:87127/1‑148 (MQ=255)
                     gAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCAt                                                                                                                         <  2:3392362/148‑1 (MQ=255)
                     gAAGTGCCGAAAGAGCTGCGTGTCGATAGTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCAt                                                                                                                         <  2:3392355/148‑1 (MQ=255)
                      aaGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCAtt                                                                                                                        >  2:1996098/1‑148 (MQ=255)
                              aaaGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGc                                                                                                                <  2:3432297/148‑1 (MQ=255)
                               nagagCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGcc                                                                                                               >  1:910049/2‑148 (MQ=255)
                                agagCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCg                                                                                                              >  2:1650287/1‑148 (MQ=255)
                                  agCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTc                                                                                                            >  1:2558090/1‑148 (MQ=255)
                                  agCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTc                                                                                                            >  1:2558094/1‑148 (MQ=255)
                                    cTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGc                                                                                                          >  1:1383074/1‑148 (MQ=255)
                                      gCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCa                                                                                                        <  2:1383074/148‑1 (MQ=255)
                                          gtCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATTCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATTAGTTTAAATATTTTCCTCTATACTGACACCGGCATTGAAGATGCCGTCGCCATTgc                                                                                                    >  2:3512260/1‑148 (MQ=255)
                                            cGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTgccg                                                                                                  <  2:3539646/148‑1 (MQ=255)
                                            cGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTgccg                                                                                                  <  2:2075741/148‑1 (MQ=255)
                                               tatTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGccgccg                                                                                               >  2:1607366/1‑148 (MQ=255)
                                                 ttCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCcgcg                                                                                             >  1:2674594/1‑148 (MQ=255)
                                                 ttCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCcgcg                                                                                             <  2:1116506/148‑1 (MQ=255)
                                                 ttCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCcgcg                                                                                             >  1:2441090/1‑148 (MQ=255)
                                                 ttCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCcgcg                                                                                             >  2:139682/1‑148 (MQ=255)
                                                      gAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGAtttt                                                                                        <  2:1244293/148‑1 (MQ=255)
                                                      gAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGAtttt                                                                                        <  1:2055896/148‑1 (MQ=255)
                                                       aaGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGAttttt                                                                                       >  1:473487/1‑148 (MQ=255)
                                                       aaGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGGTTAACTATTTTCCTCGATACTGACCCCGGCATTTACGATGCCGTCGCCATTGCCGCCGCGAttttt                                                                                       >  1:2697572/1‑148 (MQ=255)
                                                         gTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACACCGGCATTTACGATGCCGTCGCCATTGCCGCCGCGATTTTTGc                                                                                     >  2:2509087/1‑148 (MQ=255)
                                                          tCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCa                                                                                    <  1:2913926/148‑1 (MQ=255)
                                                             aTTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCAccc                                                                                 >  1:3068203/1‑148 (MQ=255)
                                                               tAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGa                                                                               <  1:801166/148‑1 (MQ=255)
                                                                   tCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTc                                                                           >  2:739134/1‑148 (MQ=255)
                                                                       tAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGAcc                                                                       >  2:135351/1‑148 (MQ=255)
                                                                       tAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGAcc                                                                       <  1:1047767/148‑1 (MQ=255)
                                                                         gcagcCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTg                                                                     <  2:817008/148‑1 (MQ=255)
                                                                         gcagcCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTg                                                                     >  1:1107120/1‑148 (MQ=255)
                                                                         gcagcCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTg                                                                     >  1:1107035/1‑148 (MQ=255)
                                                                            gcCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCaa                                                                  >  1:1672469/1‑148 (MQ=255)
                                                                            gcCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCaa                                                                  >  2:3101904/1‑148 (MQ=255)
                                                                            gcCTAAGTTATGCGAAAATGCCGGTCTTGTTACAGGCATTTTTTATGGAGAAAAAATGCGTTTACCTATTTTCCTCGATACTGACCCCGGAAATGACGATGCCGACGCCATTGCCGCCGCGATTTTTGCACACGAACTCGACCTGCaa                                                                  >  1:864394/1‑148 (MQ=255)
                                                                                aaGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACtga                                                              >  2:2859097/1‑148 (MQ=255)
                                                                                  gTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACtgatg                                                            <  1:3011024/148‑1 (MQ=255)
                                                                                   ttATGGGAATATGGCGGTCTTGTTACCGGCATTTTTTATGGAGAAATCATGCGTTTACCTATTTTCCTCGATACTGTCCCCGGCATTGACGATGACGTCGCCATTGGCGGCGCGATTTTTGCACCCGTACTCGACCTGGAACtgatga                                                           <  2:3548697/148‑1 (MQ=255)
                                                                                   ttATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACtgatga                                                           <  1:3323102/148‑1 (MQ=255)
                                                                                    tATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGac                                                          >  1:3419179/1‑148 (MQ=255)
                                                                                     aTGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGacc                                                         >  1:1808021/1‑148 (MQ=255)
                                                                                         gAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCg                                                     >  1:1243377/1‑148 (MQ=255)
                                                                                          aaaaTGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGt                                                    >  1:762063/1‑148 (MQ=255)
                                                                                          aaaaTGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGt                                                    >  1:661219/1‑148 (MQ=255)
                                                                                           aaaTGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTc                                                   >  2:3264695/1‑148 (MQ=255)
                                                                                                  ggTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTa                                            >  1:2556009/1‑148 (MQ=255)
                                                                                                  ggTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTa                                            <  1:1650287/148‑1 (MQ=255)
                                                                                                  ggTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTa                                            <  1:3138648/148‑1 (MQ=255)
                                                                                                  ggTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTa                                            >  1:577767/1‑148 (MQ=255)
                                                                                                       tGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGtc                                       <  2:444778/148‑1 (MQ=255)
                                                                                                        gTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGtct                                      <  1:838446/148‑1 (MQ=255)
                                                                                                           aCCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCgg                                   <  2:193999/148‑1 (MQ=255)
                                                                                                            ccGGCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGt                                  <  1:365678/148‑1 (MQ=255)
                                                                                                              ggCATTTTTTATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTg                                >  2:2042157/1‑148 (MQ=255)
                                                                                                                 aTTTTTTATGGAGAAAACATGCGTTTACCTTTTTTCCTCGATTCTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTgaga                             <  1:2965858/148‑1 (MQ=255)
                                                                                                                  ttttttATGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGaa                            <  2:1808021/148‑1 (MQ=255)
                                                                                                                         tGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTAcc                     <  2:3056733/148‑1 (MQ=255)
                                                                                                                         tGGAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTAcc                     >  1:2603231/1‑148 (MQ=255)
                                                                                                                          ggAGAAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTAccc                    >  2:3260954/1‑148 (MQ=255)
                                                                                                                           gagaAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCg                   >  2:1627149/1‑148 (MQ=255)
                                                                                                                           gagaAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCg                   >  2:1627166/1‑148 (MQ=255)
                                                                                                                             gaAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCa                 >  1:2036428/1‑148 (MQ=255)
                                                                                                                             gaAAACATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCa                 >  1:2036492/1‑148 (MQ=255)
                                                                                                                                aaCATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATg              >  1:2318004/1‑148 (MQ=255)
                                                                                                                                  cATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGcc            >  1:1339446/1‑148 (MQ=255)
                                                                                                                                  cATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGcc            <  1:292326/148‑1 (MQ=255)
                                                                                                                                  cATGCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGCCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGcc            <  2:2253013/148‑1 (MQ=255)
                                                                                                                                  cATGCGTTTACCAAATTTCCTCGATACTGACCCCGGCATTGACGATGCCTACGACATTGCCGCCGCGAAATTTGAACCCGAACTAGACCTGCAACTGAACACCACCGTCGCGGGTAATGTCTCGGTTGAGAACACTACACGCAAAGcc            >  2:2419767/1‑148 (MQ=255)
                                                                                                                                     gCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTg         <  1:2154942/148‑1 (MQ=255)
                                                                                                                                     gCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTg         <  2:2697572/148‑1 (MQ=255)
                                                                                                                                     gCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTg         >  2:67097/1‑148 (MQ=255)
                                                                                                                                     gCGTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTg         <  1:1607366/148‑1 (MQ=255)
                                                                                                                                       gTTTACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCa       <  1:2222192/148‑1 (MQ=255)
                                                                                                                                         ttACCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAAc     >  1:304749/1‑148 (MQ=255)
                                                                                                                                           aCCTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAActg   >  2:2258107/1‑148 (MQ=255)
                                                                                                                                            ccTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAActgc  <  2:2926140/148‑1 (MQ=255)
                                                                                                                                            ccTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAActgc  <  2:1672469/148‑1 (MQ=255)
                                                                                                                                            ccTATTTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAActgc  <  2:1670560/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGC  >  CP009273/27162‑27449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑