Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,921,245 G→T 100% intergenic (+214/‑343) ygdH → / → sdaC UPF0717 family protein/putative serine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,921,2450GT96.4% 780.0 / 17.3 259intergenic (+214/‑343)ygdH/sdaCUPF0717 family protein/putative serine transporter
Reads supporting (aligned to +/- strand):  ref base G (1/9);  new base T (130/119);  total (131/128)
Fisher's exact test for biased strand distribution p-value = 9.60e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 262 total aligned reads displayed.

ATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAATCTAT  >  CP009273/2921102‑2921389
                                                                                                                                               |                                                                                                                                                
 tttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTaa                                                                                                                                             >  2:2564003/1‑148 (MQ=255)
  ttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCTCCATTATTaaa                                                                                                                                            >  2:2973003/1‑147 (MQ=255)
   tGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACt                                                                                                                                           >  1:445852/1‑148 (MQ=255)
      tataTGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTaaa                                                                                                                                        <  2:1878307/148‑1 (MQ=255)
       atatGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTaaaa                                                                                                                                       <  2:2769944/148‑1 (MQ=255)
         atGAATGTTTCTGACCACCCGCACGCGACAAATATCATCAAAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGt                                                                                                                                     <  2:2895596/148‑1 (MQ=255)
          tGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTa                                                                                                                                    <  1:2986033/148‑1 (MQ=255)
           gAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTAt                                                                                                                                   <  1:3187175/148‑1 (MQ=255)
            aaTGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTAtt                                                                                                                                  <  2:269931/148‑1 (MQ=255)
            aaTGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTAtt                                                                                                                                  <  2:194835/148‑1 (MQ=255)
              tGTTTCTTGCCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGa                                                                                                                                >  2:1283429/1‑148 (MQ=255)
              tGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGa                                                                                                                                <  2:42082/148‑1 (MQ=255)
               gTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGAt                                                                                                                               >  1:3379735/1‑148 (MQ=255)
                 ttCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGAttt                                                                                                                             >  1:2197147/1‑148 (MQ=255)
                  tCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGAtttt                                                                                                                            >  1:2247241/1‑148 (MQ=255)
                     tACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTc                                                                                                                         <  1:257818/148‑1 (MQ=255)
                          cccTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTc                                                                                                                    >  2:2093014/1‑148 (MQ=255)
                           ccTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCa                                                                                                                   >  2:583843/1‑148 (MQ=255)
                              cACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAAcc                                                                                                                <  2:3121356/148‑1 (MQ=255)
                              cACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAAcc                                                                                                                >  1:1379490/1‑148 (MQ=255)
                               aCGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCt                                                                                                               >  1:1849916/1‑148 (MQ=255)
                                cgcgACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTa                                                                                                              >  1:1930298/1‑148 (MQ=255)
                                    aCAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAAAATACata                                                                                                          >  2:1830822/1‑148 (MQ=255)
                                      aaaTATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACatata                                                                                                        <  2:2812053/148‑1 (MQ=255)
                                       aaTATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACatatat                                                                                                       >  2:3022378/1‑148 (MQ=255)
                                       aaTATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACatatat                                                                                                       <  1:2520441/148‑1 (MQ=255)
                                       aaTATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACatatat                                                                                                       >  1:6765/1‑148 (MQ=255)
                                         tatCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTg                                                                                                     >  2:2914091/1‑148 (MQ=255)
                                           tcatcaCAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTgcg                                                                                                   >  1:180685/1‑148 (MQ=255)
                                           tcatcaCAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTgcg                                                                                                   >  2:2908905/1‑148 (MQ=255)
                                           tcatcaCAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTgcg                                                                                                   >  2:683825/1‑148 (MQ=255)
                                                accgTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGccc                                                                                              <  1:2425075/145‑1 (MQ=255)
                                                    ttAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGga                                                                                          >  2:1081451/1‑148 (MQ=255)
                                                        tatGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGgaagaa                                                                                      >  1:547510/1‑148 (MQ=255)
                                                        tatGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGgaagaa                                                                                      >  1:1309097/1‑148 (MQ=255)
                                                         atGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGaagaag                                                                                     >  2:249296/1‑148 (MQ=255)
                                                           gTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTc                                                                                   <  2:3530360/148‑1 (MQ=255)
                                                            tCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCa                                                                                  <  1:274117/148‑1 (MQ=255)
                                                              aTACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGa                                                                                <  1:1248515/148‑1 (MQ=255)
                                                              aTACAATTTATGTTGCAACGAAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGATATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGa                                                                                <  2:2777033/148‑1 (MQ=255)
                                                                aCAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATg                                                                              >  1:3531858/1‑148 (MQ=255)
                                                                aCAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATg                                                                              <  2:283702/148‑1 (MQ=255)
                                                                    tttATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGt                                                                          <  1:1565500/148‑1 (MQ=255)
                                                                     ttATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGtt                                                                         <  1:2892978/148‑1 (MQ=255)
                                                                     ttATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGtt                                                                         <  1:1035907/148‑1 (MQ=255)
                                                                       aTGTTGCAACGCAAAGGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTa                                                                       <  1:1976172/148‑1 (MQ=255)
                                                                         gTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAAt                                                                     <  1:2243727/148‑1 (MQ=255)
                                                                           tGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATgg                                                                   >  1:423332/1‑148 (MQ=255)
                                                                           tGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATgg                                                                   <  1:2732635/148‑1 (MQ=255)
                                                                            gCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATggg                                                                  >  1:2218240/1‑148 (MQ=255)
                                                                               aCGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCaa                                                               <  2:1407320/148‑1 (MQ=255)
                                                                                 gCAAACGTTGCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAACTTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAat                                                             <  1:2541497/148‑1 (MQ=255)
                                                                                  cAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAata                                                            >  2:2567549/1‑148 (MQ=255)
                                                                                  cAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAata                                                            >  1:828612/1‑148 (MQ=255)
                                                                                  cAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAata                                                            <  1:998493/148‑1 (MQ=255)
                                                                                   aaaCGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAatat                                                           <  1:2033627/148‑1 (MQ=255)
                                                                                     aCGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTg                                                         >  1:761598/1‑148 (MQ=255)
                                                                                     aCGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTg                                                         >  1:1167320/1‑148 (MQ=255)
                                                                                       gTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGcc                                                       >  2:3389535/1‑148 (MQ=255)
                                                                                         ttCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATCTTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCt                                                     >  1:928261/1‑148 (MQ=255)
                                                                                          tCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCtt                                                    <  2:983959/148‑1 (MQ=255)
                                                                                            ccTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTaa                                                  >  2:797297/1‑148 (MQ=255)
                                                                                             cTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTaaa                                                 <  1:292962/148‑1 (MQ=255)
                                                                                               aTTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAAtt                                               <  2:315292/148‑1 (MQ=255)
                                                                                                 tttCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATtct                                             <  1:2384766/148‑1 (MQ=255)
                                                                                                 tttCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATtct                                             <  1:596335/148‑1 (MQ=255)
                                                                                                  ttCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATtctc                                            <  1:1674145/148‑1 (MQ=255)
                                                                                                  ttCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATtctc                                            <  2:2400866/148‑1 (MQ=255)
                                                                                                      tAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCtttt                                        <  1:1364550/148‑1 (MQ=255)
                                                                                                       aaaCCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTa                                       >  1:2071920/1‑148 (MQ=255)
                                                                                                        aaCCGTATTTTATCGCTATGAAAAAGAAATTATCTCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTAc                                      >  1:1931657/1‑148 (MQ=255)
                                                                                                           cGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACttt                                   >  2:830446/1‑148 (MQ=255)
                                                                                                           cGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGACAAATATTGCCCTTAAATTCTCTTTTACttt                                   >  2:1731815/1‑148 (MQ=255)
                                                                                                             tATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTg                                 <  1:2908905/148‑1 (MQ=255)
                                                                                                              aTTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGa                                <  2:1849916/148‑1 (MQ=255)
                                                                                                              aTTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGa                                <  2:423332/148‑1 (MQ=255)
                                                                                                                tttATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGAtt                              <  1:2239862/148‑1 (MQ=255)
                                                                                                                  tatctatATTAAAAATAAATTATCGCAATTATTAACTAAAAGTATTTATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTa                            <  2:3306977/142‑1 (MQ=255)
                                                                                                                  tATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTa                            <  2:3379735/148‑1 (MQ=255)
                                                                                                                   aTCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTAc                           <  2:296213/148‑1 (MQ=255)
                                                                                                                     cGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACag                         >  2:547846/1‑148 (MQ=255)
                                                                                                                      gCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACaga                        <  2:2208908/148‑1 (MQ=255)
                                                                                                                         attaaaaaGAAATTATAGCCATTATTAAATAAAATTTTTGATTTTTTCAGTTCAACCTACTTATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTa                     <  1:2564082/145‑1 (MQ=255)
                                                                                                                         aTGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTa                     >  2:2053796/1‑148 (MQ=255)
                                                                                                                          tGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTaa                    <  1:2805740/148‑1 (MQ=255)
                                                                                                                           gAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTaaa                   >  1:1031616/1‑148 (MQ=255)
                                                                                                                              aaaGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCg                <  1:3546165/148‑1 (MQ=255)
                                                                                                                              aaaGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCg                >  2:1651473/1‑148 (MQ=255)
                                                                                                                               aaGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGt               >  1:3323783/1‑148 (MQ=255)
                                                                                                                                aGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGtt              <  2:1221513/148‑1 (MQ=255)
                                                                                                                                aGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGtt              <  2:1221520/148‑1 (MQ=255)
                                                                                                                                 gAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTg             >  1:2671658/1‑148 (MQ=255)
                                                                                                                                    aTTATCGCCATTATTAACTAAAAGTATTGATTTTTTCATTTCAAAATACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGa          <  1:1830822/148‑1 (MQ=255)
                                                                                                                                    aTTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGa          >  1:1640688/1‑148 (MQ=255)
                                                                                                                                     ttATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGAt         >  2:1530303/1‑148 (MQ=255)
                                                                                                                                      tATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATa        >  1:2086214/1‑148 (MQ=255)
                                                                                                                                      tATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTAAAGAGTAAAGCGTTGGGATa        >  2:2450089/1‑148 (MQ=255)
                                                                                                                                       aTCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATaa       >  1:3037641/1‑148 (MQ=255)
                                                                                                                                         cGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatc     <  2:2231555/148‑1 (MQ=255)
                                                                                                                                           ccATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatcta   <  1:583515/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
ATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAATCTAT  >  CP009273/2921102‑2921389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑