BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,940,854	G→T	11.4%	intergenic (+32/‑2)	metZ → / → metW	tRNA‑Met/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,940,854	0	G	T	11.4%	339.6 / 20.1	120	intergenic (+32/‑2)	metZ/metW	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base G (49/57); new base T (8/6); total (57/63)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

GCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCA  >  CP009273/2940707‑2941001

gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTTTTGAAGTAAAGCAGTACGGTg                                                                                                                                                     <  2:423472/148‑1 (MQ=255)

 cATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGa                                                                                                                                                    <  2:1561711/148‑1 (MQ=255)

   tGAAAACTCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGACGTAAAGCAGTACGGTGAcg                                                                                                                                                  >  1:2368308/1‑148 (MQ=255)

   tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAGTTTAATCAACTCAAGCAGGACTCTGAcg                                                                                                                                                  >  1:1887366/1‑148 (MQ=18)

      aaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATAAGAGCAAGTACAGCAGTACTGTTACGCgg                                                                                                                                               >  2:2703839/1‑148 (MQ=18)

       aaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAATTTTGAGTAAGTAAAGCAGTACGGTTACGCggg                                                                                                                                              >  1:736050/1‑148 (MQ=18)

        aCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCgggg                                                                                                                                             >  2:2007957/1‑148 (MQ=255)

         cgcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGt                                                                                                                                            <  1:3247185/148‑1 (MQ=255)

            gAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTCCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGa                                                                                                                                         <  1:2735507/148‑1 (MQ=255)

             aGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGag                                                                                                                                        <  1:756648/148‑1 (MQ=255)

                  aGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                                                   <  1:2906020/148‑1 (MQ=255)

                  aGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                                                   <  1:1564573/148‑1 (MQ=255)

                    gataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTg                                                                                                                                 >  1:1411284/2‑148 (MQ=255)

                     ataGTGCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTgg                                                                                                                                <  2:3239087/148‑1 (MQ=255)

                     ataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAACTTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTgg                                                                                                                                <  1:2493531/148‑1 (MQ=255)

                     ataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTgg                                                                                                                                <  2:3239085/148‑1 (MQ=255)

                     ataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTgg                                                                                                                                <  2:226970/148‑1 (MQ=255)

                     ataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTgg                                                                                                                                <  2:226910/148‑1 (MQ=255)

                        gTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAg                                                                                                                             <  2:3219329/148‑1 (MQ=255)

                           cgcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtc                                                                                                                          <  1:1147006/148‑1 (MQ=255)

                           cgcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtc                                                                                                                          <  1:1147013/148‑1 (MQ=255)

                            gcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcg                                                                                                                         <  1:3560266/148‑1 (MQ=255)

                            gcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcg                                                                                                                         <  1:3561465/148‑1 (MQ=255)

                                 cACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg                                                                                                                    <  2:3547745/148‑1 (MQ=255)

                                 cACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg                                                                                                                    <  2:1655358/148‑1 (MQ=255)

                                 cACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg                                                                                                                    <  2:911720/148‑1 (MQ=255)

                                    ggACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTc                                                                                                                 <  1:599143/148‑1 (MQ=255)

                                     gACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                                                                <  2:1155669/148‑1 (MQ=40)

                                     gACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                                                                <  2:2971485/148‑1 (MQ=40)

                                      aCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAt                                                                                                               <  2:1645466/148‑1 (MQ=40)

                                       cgcgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATa                                                                                                              <  1:1874712/148‑1 (MQ=255)

                                          ggggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAcc                                                                                                           <  2:1189393/148‑1 (MQ=40)

                                           gggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGAATAAAAGCCGTACGGGGACGGGGGGTGGAGCAGCCTGGTAGCGCGTCGGGCTCAAAAccc                                                                                                          >  2:118289/1‑148 (MQ=11)

                                            ggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                                                                                         >  1:1090359/1‑148 (MQ=40)

                                                  gcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                                                                                                   <  2:3509035/148‑1 (MQ=40)

                                                  gcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                                                                                                   <  2:3508849/148‑1 (MQ=40)

                                                     gcCTGGTAGCTAGTCGGGATCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGTTGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgtc                                                                                                <  2:2541536/148‑1 (MQ=255)

                                                        tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGt                                                                                             <  2:628255/148‑1 (MQ=40)

                                                        tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGt                                                                                             <  2:628122/148‑1 (MQ=40)

                                                        tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGt                                                                                             <  2:628071/148‑1 (MQ=40)

                                                             gCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATATTTTGATGAAGTAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCaaa                                                                                        <  2:2801746/148‑1 (MQ=255)

                                                              cTCGTCGGGCTAATAAGCCGAAGGTCGGCGGTTCACATCCGGCCCCCGCAACCAATTACCTTTTGATGAAGTATAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAt                                                                                       <  2:2719054/148‑1 (MQ=11)

                                                                  tcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCgg                                                                                   >  2:1690199/1‑148 (MQ=40)

                                                                            aaCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc                                                                         >  1:1075885/1‑148 (MQ=40)

                                                                             aCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAcc                                                                        >  2:2901188/1‑148 (MQ=40)

                                                                              cccTAAGGTCGTCGGTTCAAATCCGTCCCCCGCAACGAATTACATTTGGCTGAAGTCAAGCCCTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                                       <  2:3426749/148‑1 (MQ=11)

                                                                              cccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                                       >  2:2614604/1‑148 (MQ=40)

                                                                              cccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                                       >  1:2577231/1‑148 (MQ=40)

                                                                              cccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTCGCGGTAGAGGGGAGGTACGGTGAGGCGCGGTGGGGGAGCGTGGTTGGACGACGGGCTCAAAACCCCACGGGCGGCGGTTCAAATCCGGCCCCCGcggccc                                                                       >  2:2089279/1‑143 (MQ=2)

                                                                                    ggTCGTCGGTTCTAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaa                                                                 <  2:963771/148‑1 (MQ=255)

                                                                                     gtcgtcGGTTCAAATCCGTCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                                                <  2:2682035/148‑1 (MQ=255)

                                                                                     gtcgtcGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                                                <  1:1955842/148‑1 (MQ=255)

                                                                                        ttctgTTCAAATCCTGCCCCCGCAACCAAATAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtg                                                             <  2:1681635/144‑1 (MQ=255)

                                                                                         tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtga                                                            <  1:1408180/148‑1 (MQ=255)

                                                                                         tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAtttaga                                                           >  2:2407415/1‑145 (MQ=255)

                                                                                         tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGAAGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGACGGTCGTCGGTTCAAATCCGGCCCCCGCAACCACTCAAAGTtga                                                            >  1:2912348/1‑148 (MQ=255)

                                                                                         tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAGGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGGAGCACGTCGGGCGCATAACCCGGAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCCCTCAAAtgtca                                                            >  1:2912440/1‑144 (MQ=14)

                                                                                         tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATATGAGGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCGGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCACTCaaagaaca                                                            >  2:3021283/1‑143 (MQ=14)

                                                                                            gTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtgatga                                                         <  2:1090359/148‑1 (MQ=255)

                                                                                            gTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtgatga                                                         <  1:1577974/148‑1 (MQ=255)

                                                                                             ttCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCACTCAAATTTGATGaa                                                        >  1:2950813/1‑148 (MQ=255)

                                                                                             ttCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAAATTTGAGGaa                                                        >  1:1172533/1‑148 (MQ=255)

                                                                                               cataTCCGGCCCCCGCAACCAATTATAATTTGCTGAAGTATCGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGt                                                      <  1:1197443/145‑1 (MQ=17)

                                                                                               cAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGt                                                      <  1:1854517/148‑1 (MQ=255)

                                                                                               cAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGt                                                      <  1:1197439/148‑1 (MQ=255)

                                                                                                 aaTCCGGACCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTaa                                                    <  2:1785342/148‑1 (MQ=255)

                                                                                                   tCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCACATCCGGCCCCCGCAACCAATCAAATTTGAGGAAGTaaaa                                                  >  1:3490802/1‑148 (MQ=255)

                                                                                                   tCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATATCATGACGTagaa                                                  >  2:2230629/1‑148 (MQ=255)

                                                                                                   tCCGGCCCCCGCAACCAATTAAAATTTGAGGACGTAAAGCAGTACGGTGACGCGGGGGGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAGTCATATTTGATGCAGtcccg                                                  >  2:2230608/1‑144 (MQ=14)

                                                                                                       gCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGATAGATGAGGAAGTAAGAGctg                                              >  1:501416/1‑146 (MQ=14)

                                                                                                       gCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCATATTTGAGGATGTaatatctg                                              >  1:502822/1‑142 (MQ=14)

                                                                                                       gCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGCAGTAAGAGCAg                                              >  1:380583/1‑148 (MQ=255)

                                                                                                       gCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAg                                              >  1:949307/1‑148 (MQ=255)

                                                                                                        cccccGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGACGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCCAAGTTGGGGAAGTAAAAGCAgg                                             >  2:1941297/1‑147 (MQ=17)

                                                                                                         ccccGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTa                                            >  2:1531301/1‑148 (MQ=255)

                                                                                                             gCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGt                                        <  2:2692282/148‑1 (MQ=255)

                                                                                                                aCCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCACTCAAATTTGATGAAGTAAAAGCAGTACGGTGAc                                     >  1:3235342/1‑148 (MQ=255)

                                                                                                                 ccAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAAGAAGTAAATGCAGTAAGGTGAcg                                    >  2:1054344/1‑148 (MQ=255)

                                                                                                                     ttAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCggg                                <  1:2007957/148‑1 (MQ=255)

                                                                                                                     ttAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATATCAGCAAGTAACAGCTGTACTGTGTAGCggg                                >  2:1879644/1‑148 (MQ=14)

                                                                                                                       aaaaTTTGATGAAGTAAAGCATTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCTCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGt                              <  2:92651/148‑1 (MQ=255)

                                                                                                                       aaaaTTTGATGAAGTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGt                              >  1:1412064/1‑148 (MQ=255)

                                                                                                                           tttGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAATTTAGAGCAAGTAAAGGCATTAAGGTGATGCGGGGTGGat                          >  2:149567/1‑147 (MQ=17)

                                                                                                                            ttGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACACGGGGTGGagc                         >  1:1512983/1‑148 (MQ=255)

                                                                                                                                  tagacttgCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTg                   <  2:2526795/141‑1 (MQ=255)

                                                                                                                                    gTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGt                 <  2:1412064/148‑1 (MQ=255)

                                                                                                                                    gTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGt                 <  2:3560187/148‑1 (MQ=255)

                                                                                                                                     tAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAGGAAGTAAAAGCAGTACGGTGACGAGGGGTGGAGCAGCCTGGTa                >  2:1002962/1‑148 (MQ=255)

                                                                                                                                     tAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAGTTCATGCAGTAAAAGCAGTACGGTGACGCGGGGTGGAGAAGCCTGGTa                >  2:1002971/1‑148 (MQ=255)

                                                                                                                                      ttcggattACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAg               <  2:1191686/141‑1 (MQ=255)

                                                                                                                                        aGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAATTTTCAGGAAGTACAAGCAGTAAGGTTACGCGGGGTGGAGCAGCCTGGTAGCt             >  2:93763/1‑148 (MQ=14)

                                                                                                                                        aGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAGTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt             >  1:512420/1‑148 (MQ=255)

                                                                                                                                          cAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcg           >  1:3237456/1‑148 (MQ=255)

                                                                                                                                          cAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTCATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcg           >  2:1924816/1‑148 (MQ=255)

                                                                                                                                           aGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgt          <  1:2901188/148‑1 (MQ=255)

                                                                                                                                            gTACGGTTCCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCACATTTGAGGAAGTAACAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGGCGCtcgtc         >  2:2824625/1‑148 (MQ=14)

                                                                                                                                            gTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTATAAGCAGTACGGTGACGAGGGGTGGAGCAGCCTGGTAGCtcgtc         >  2:2826683/1‑148 (MQ=255)

                                                                                                                                            gTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAGGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtc         >  2:2395374/1‑148 (MQ=255)

                                                                                                                                             tACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg        >  1:140682/1‑148 (MQ=255)

                                                                                                                                                   gACGCGGGGTGGAGCAGCCTGGTCGCTGGTCGGGCTCAGAACCCGAAGGTGGTCGCTTCCAATCCCGCCCGGGCAACGAATAACAGTTGGGGGTCTACTTGGCCTCCGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa  <  2:2265553/148‑1 (MQ=2)

GCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCA  >  CP009273/2940707‑2941001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`