Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 250,909 A→T 100% L432Q (CTG→CAG)  pepD ← aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273250,9090AT97.4% 703.7 / 5.5 225L432Q (CTG→CAG) pepDaminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base T (121/98);  total (124/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 225 total aligned reads displayed.

TTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTT  >  CP009273/250765‑251053
                                                                                                                                                |                                                                                                                                                
ttcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGc                                                                                                                                               >  1:2558863/1‑148 (MQ=255)
ttcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGc                                                                                                                                               >  1:2052421/1‑148 (MQ=255)
    gcagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCcgc                                                                                                                                           >  1:32694/1‑148 (MQ=255)
        ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTgg                                                                                                                                       >  1:3392081/1‑148 (MQ=255)
        ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTgg                                                                                                                                       >  1:508755/1‑148 (MQ=255)
        ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTgg                                                                                                                                       <  1:172810/148‑1 (MQ=255)
        ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTgg                                                                                                                                       <  2:3545647/148‑1 (MQ=255)
         tcagtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGa                                                                                                                                      >  2:697860/1‑148 (MQ=255)
         tcagtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGa                                                                                                                                      <  1:688292/148‑1 (MQ=255)
         tcagtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGa                                                                                                                                      <  1:1966093/148‑1 (MQ=255)
          cagtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGAt                                                                                                                                     <  2:2817012/148‑1 (MQ=255)
           agtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATa                                                                                                                                    <  1:2151186/148‑1 (MQ=255)
           agtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATa                                                                                                                                    <  2:312191/148‑1 (MQ=255)
             tcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAAt                                                                                                                                  <  2:3387215/148‑1 (MQ=255)
                 cagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTgg                                                                                                                              >  2:760878/1‑148 (MQ=255)
                 cagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTgg                                                                                                                              >  2:705031/1‑148 (MQ=255)
                 cagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTgg                                                                                                                              <  1:113129/148‑1 (MQ=255)
                    tgtCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATg                                                                                                                           >  2:3500861/1‑148 (MQ=255)
                    tgtCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATg                                                                                                                           >  2:42059/1‑148 (MQ=255)
                          gTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGc                                                                                                                     >  1:893296/1‑148 (MQ=255)
                             aTGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTc                                                                                                                  <  2:1610188/148‑1 (MQ=255)
                              tGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCt                                                                                                                 >  1:3032269/1‑148 (MQ=255)
                                aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttg                                                                                                               <  1:1256538/148‑1 (MQ=255)
                                    aCGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCCTGTCCATTTCCGGATACGGTTTTTTGAACAGACCACAGTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                           <  2:2582019/148‑1 (MQ=255)
                                    aCGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                           <  1:2137329/148‑1 (MQ=255)
                                     cGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGaa                                                                                                          >  1:692671/1‑148 (MQ=255)
                                      gCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAAc                                                                                                         <  1:2809272/148‑1 (MQ=255)
                                             aTGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCt                                                                                                  >  1:2776369/1‑148 (MQ=255)
                                                tgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGAt                                                                                               <  1:3462139/148‑1 (MQ=255)
                                                tgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGAt                                                                                               <  2:122597/148‑1 (MQ=255)
                                                 gAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATa                                                                                              >  1:3087367/1‑148 (MQ=255)
                                                    cTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGt                                                                                           <  2:3254664/148‑1 (MQ=255)
                                                     ttGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGTATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGtt                                                                                          >  1:958200/1‑148 (MQ=255)
                                                     ttGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGTATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGtt                                                                                          >  1:361733/1‑148 (MQ=255)
                                                      tGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGTATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGttt                                                                                         >  1:1617820/1‑148 (MQ=255)
                                                      tGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGttt                                                                                         >  1:730846/1‑148 (MQ=255)
                                                       gCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTc                                                                                        <  2:1465886/148‑1 (MQ=255)
                                                         tCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCAc                                                                                      <  2:904431/148‑1 (MQ=255)
                                                           aTCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGt                                                                                    >  2:2675998/1‑148 (MQ=255)
                                                                    gtgtGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGc                                                                           <  2:3208471/148‑1 (MQ=255)
                                                                     tgtgGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCa                                                                          <  1:2531105/148‑1 (MQ=255)
                                                                           ccGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCg                                                                    >  2:2756020/1‑148 (MQ=255)
                                                                           ccGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCg                                                                    <  1:760878/148‑1 (MQ=255)
                                                                             ggTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGga                                                                  >  1:2193242/1‑148 (MQ=255)
                                                                                gATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGaa                                                               <  1:450437/148‑1 (MQ=255)
                                                                                 aTAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTCCCAGATGCATCACCGGAGAAt                                                              >  2:2227718/1‑148 (MQ=255)
                                                                                 aTAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAAt                                                              <  2:360566/148‑1 (MQ=255)
                                                                                 aTAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAAt                                                              <  2:1597196/148‑1 (MQ=255)
                                                                                 aTAGTTGGCCCGATAGAAACCATGTCCATGTCCGGATACGGTTTTTTGAACAGACCACAGTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAAt                                                              <  2:3355154/148‑1 (MQ=255)
                                                                                   aGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTa                                                            >  2:1957490/1‑148 (MQ=255)
                                                                                    gTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAg                                                           <  1:2009421/148‑1 (MQ=255)
                                                                                     ttGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGc                                                          >  2:1069303/1‑148 (MQ=255)
                                                                                     ttGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGc                                                          >  2:1496864/1‑148 (MQ=255)
                                                                                     ttGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGc                                                          >  2:888412/1‑148 (MQ=255)
                                                                                      tGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCg                                                         <  1:503584/148‑1 (MQ=255)
                                                                                           ctaTAGAAACCATGTCCATTTCCGGATACGTTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCgg                                                    <  2:37721/146‑1 (MQ=255)
                                                                                           cGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGTATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCgg                                                    <  1:792966/148‑1 (MQ=255)
                                                                                           cGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCgg                                                    <  2:856713/148‑1 (MQ=255)
                                                                                           cGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCgg                                                    >  2:2386857/1‑148 (MQ=255)
                                                                                              tAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACGTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTg                                                 >  2:20864/1‑148 (MQ=255)
                                                                                              tAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTg                                                 >  2:2436472/1‑148 (MQ=255)
                                                                                               aGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgc                                                >  1:909408/1‑148 (MQ=255)
                                                                                               aGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgc                                                >  1:411970/1‑148 (MQ=255)
                                                                                                   aCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgccagc                                            >  2:1859316/1‑148 (MQ=255)
                                                                                                   aCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgccagc                                            <  1:1476271/148‑1 (MQ=255)
                                                                                                   aCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgccagc                                            >  1:1901798/1‑148 (MQ=255)
                                                                                                    ccATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgccagcc                                           >  2:3458515/1‑148 (MQ=255)
                                                                                                      aTGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGccagccag                                         >  2:1896133/1‑148 (MQ=255)
                                                                                                       tGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGTTGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAgg                                        <  1:2628770/148‑1 (MQ=255)
                                                                                                       tGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAgg                                        <  2:3193762/148‑1 (MQ=255)
                                                                                                        gTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGa                                       >  1:1770333/1‑148 (MQ=255)
                                                                                                           cATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGatat                                    >  2:1926615/1‑148 (MQ=255)
                                                                                                           cATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGatat                                    >  2:2503842/1‑148 (MQ=255)
                                                                                                           cATTTCCGGATACGGTTTTTTGAACAGACCACAGTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGatat                                    <  2:3498602/148‑1 (MQ=255)
                                                                                                            aTTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATg                                   >  2:211349/1‑148 (MQ=255)
                                                                                                                 cGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCt                              <  2:2014394/148‑1 (MQ=255)
                                                                                                                 cGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCt                              <  2:1743148/148‑1 (MQ=255)
                                                                                                                  ggATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCtt                             >  1:3381864/1‑148 (MQ=255)
                                                                                                                   gATACGGTTTTTTTAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTTATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCttt                            <  1:32192/148‑1 (MQ=255)
                                                                                                                      aCGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGc                         >  1:3145900/1‑148 (MQ=255)
                                                                                                                      aCGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGc                         >  2:677567/1‑148 (MQ=255)
                                                                                                                      aCGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGc                         <  2:564569/148‑1 (MQ=255)
                                                                                                                       cGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCt                        <  2:508755/148‑1 (MQ=255)
                                                                                                                            ttttGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGt                   <  1:2404539/148‑1 (MQ=255)
                                                                                                                             tttGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGtt                  <  2:3392081/148‑1 (MQ=255)
                                                                                                                              ttGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGttt                 <  1:2439609/148‑1 (MQ=255)
                                                                                                                                   cAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTcgcg            >  1:347068/1‑148 (MQ=255)
                                                                                                                                    aGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGGACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCgcgc           <  1:651514/148‑1 (MQ=255)
                                                                                                                                      aCCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgcca         >  1:2733991/1‑148 (MQ=255)
                                                                                                                                      aCCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgcca         >  2:1694081/1‑148 (MQ=255)
                                                                                                                                       ccACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgccag        >  2:1526632/1‑148 (MQ=255)
                                                                                                                                        cacaTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgccagc       >  2:1788860/1‑148 (MQ=255)
                                                                                                                                        cacaTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgccagc       >  1:2291634/1‑148 (MQ=255)
                                                                                                                                         acaTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgccagcc      >  2:79640/1‑148 (MQ=255)
                                                                                                                                            ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACCGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGt   <  1:1349627/148‑1 (MQ=255)
                                                                                                                                            ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGt   <  1:3125345/148‑1 (MQ=255)
                                                                                                                                            ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGt   >  2:3065915/1‑148 (MQ=255)
                                                                                                                                             tCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGtt                  <  1:127563/132‑1 (MQ=255)
                                                                                                                                             tCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGtt                  >  2:127563/1‑132 (MQ=255)
                                                                                                                                             tCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGtt  >  1:3502435/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTT  >  CP009273/250765‑251053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑