Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,018,544 G→T 100% A279S (GCC→TCC)  xanQ → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,018,5440GT96.4% 642.4 / 10.8 209A279S (GCC→TCC) xanQxanthine permease
Reads supporting (aligned to +/- strand):  ref base G (3/5);  new base T (89/112);  total (92/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 209 total aligned reads displayed.

GGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATG  >  CP009273/3018399‑3018688
                                                                                                                                                 |                                                                                                                                                
ggtggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTcc                                                                                                                                                <  2:1355123/148‑1 (MQ=255)
 gtggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCa                                                                                                                                               >  1:1038982/1‑148 (MQ=255)
 gtggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCa                                                                                                                                               <  2:451592/148‑1 (MQ=255)
 gtggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCa                                                                                                                                               <  1:1918583/148‑1 (MQ=255)
    gATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACgg                                                                                                                                            <  2:127409/148‑1 (MQ=255)
       ttCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCaa                                                                                                                                         >  1:1365552/1‑148 (MQ=255)
           gcagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGt                                                                                                                                     <  1:3004257/148‑1 (MQ=255)
           gcagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGt                                                                                                                                     <  1:3561013/148‑1 (MQ=255)
           gcagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGt                                                                                                                                     <  1:1273455/148‑1 (MQ=255)
           gcagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGt                                                                                                                                     >  2:3228382/1‑148 (MQ=255)
            cagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGtt                                                                                                                                    <  2:1126143/148‑1 (MQ=255)
              gTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTc                                                                                                                                  >  2:1375658/1‑148 (MQ=255)
              gTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTc                                                                                                                                  <  1:3390302/148‑1 (MQ=255)
              gTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTc                                                                                                                                  <  1:1209959/148‑1 (MQ=255)
                     cAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCcgccgc                                                                                                                           >  2:917443/1‑148 (MQ=255)
                     cAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCcgccgc                                                                                                                           >  2:2087810/1‑148 (MQ=255)
                       aTTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGccc                                                                                                                         >  2:468146/1‑148 (MQ=255)
                       aTTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGccc                                                                                                                         <  2:1109508/148‑1 (MQ=255)
                        tttGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGcccc                                                                                                                        >  2:845254/1‑148 (MQ=255)
                                tAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAggg                                                                                                                <  1:3350737/148‑1 (MQ=255)
                                 aaTCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAgggg                                                                                                               <  2:1201919/148‑1 (MQ=255)
                                 aaTCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAgggg                                                                                                               <  2:2476111/148‑1 (MQ=255)
                                  aTCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAggggg                                                                                                              <  1:192689/148‑1 (MQ=255)
                                    cACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGaa                                                                                                            <  1:2283252/148‑1 (MQ=255)
                                    cACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGaa                                                                                                            <  2:645050/148‑1 (MQ=255)
                                      ccATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAaga                                                                                                          <  2:3128759/148‑1 (MQ=255)
                                      ccATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAaga                                                                                                          <  1:1648039/148‑1 (MQ=255)
                                       cATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAagag                                                                                                         >  2:1946794/1‑148 (MQ=255)
                                       cATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAagag                                                                                                         <  1:677869/148‑1 (MQ=255)
                                        aTCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGt                                                                                                        <  2:2014466/148‑1 (MQ=255)
                                        aTCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGt                                                                                                        <  1:2473469/148‑1 (MQ=255)
                                         tCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGACGAGTa                                                                                                       >  1:2134508/1‑148 (MQ=255)
                                         tCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTa                                                                                                       <  1:768717/148‑1 (MQ=255)
                                         tCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTa                                                                                                       >  1:1813177/1‑148 (MQ=255)
                                         tCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTa                                                                                                       >  1:1750370/1‑148 (MQ=255)
                                          cccGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTAt                                                                                                      >  2:219080/1‑148 (MQ=255)
                                          cccGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTAt                                                                                                      <  1:3390411/148‑1 (MQ=255)
                                            cGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCa                                                                                                    >  1:3097715/1‑148 (MQ=255)
                                             gCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAg                                                                                                   >  1:325672/1‑148 (MQ=255)
                                              cATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGt                                                                                                  <  2:485184/148‑1 (MQ=255)
                                               aTCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTc                                                                                                 <  1:3184313/148‑1 (MQ=255)
                                                 ccGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTCTCAGTccc                                                                                               >  2:452394/1‑148 (MQ=255)
                                                  cGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCg                                                                                              >  2:559215/1‑148 (MQ=255)
                                                  cGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCg                                                                                              >  2:559169/1‑148 (MQ=255)
                                                         aTACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGaaa                                                                                       >  1:2864733/1‑148 (MQ=255)
                                                          tACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAg                                                                                      >  2:2287923/1‑148 (MQ=255)
                                                           aCGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAgg                                                                                     >  1:137437/1‑148 (MQ=255)
                                                             ggCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAggcg                                                                                   <  2:1888523/148‑1 (MQ=255)
                                                               cTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAggcggc                                                                                 >  2:3534866/1‑148 (MQ=255)
                                                                tttAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGgcggcg                                                                                >  2:3229587/1‑148 (MQ=255)
                                                                  tAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTg                                                                              <  1:1100531/148‑1 (MQ=255)
                                                                   aGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGc                                                                             <  2:871880/148‑1 (MQ=255)
                                                                    gTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCt                                                                            >  2:3209135/1‑148 (MQ=255)
                                                                    gTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCt                                                                            <  2:2332759/148‑1 (MQ=255)
                                                                         aGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAg                                                                       <  2:2803591/148‑1 (MQ=255)
                                                                          gCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGa                                                                      <  2:152764/148‑1 (MQ=255)
                                                                           cTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGAt                                                                     <  2:1038982/148‑1 (MQ=255)
                                                                           cTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGAt                                                                     >  1:3099450/1‑148 (MQ=255)
                                                                             tCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATgg                                                                   <  1:2427939/148‑1 (MQ=255)
                                                                                  cAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTgg                                                              <  1:2332913/148‑1 (MQ=255)
                                                                                  cAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTgg                                                              >  1:3222435/1‑148 (MQ=255)
                                                                                   aGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGt                                                             <  1:2773369/148‑1 (MQ=255)
                                                                                    gTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGtt                                                            >  2:1620157/1‑148 (MQ=255)
                                                                                     ttCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGttt                                                           >  2:3530165/1‑148 (MQ=255)
                                                                                     ttCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGttt                                                           >  2:2832724/1‑148 (MQ=255)
                                                                                        cgggtggtTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCCCCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTg                                                        <  1:105918/146‑1 (MQ=255)
                                                                                        cTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTg                                                        <  1:105541/148‑1 (MQ=255)
                                                                                        cTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTg                                                        <  1:19921/148‑1 (MQ=255)
                                                                                         tggtggTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGt                                                       <  1:774212/148‑1 (MQ=255)
                                                                                         tggtggTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGt                                                       <  1:384820/148‑1 (MQ=255)
                                                                                               ttGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGc                                                 >  2:1775127/1‑148 (MQ=255)
                                                                                                tGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGcc                                                >  1:1071330/1‑148 (MQ=255)
                                                                                                  gCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTc                                              >  1:2527077/1‑148 (MQ=255)
                                                                                                   cACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTcc                                             >  1:1434818/1‑148 (MQ=255)
                                                                                                     cGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGc                                           >  1:1998401/1‑148 (MQ=255)
                                                                                                       aTTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTg                                         <  1:2058721/148‑1 (MQ=255)
                                                                                                            tCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGt                                    >  2:2648953/1‑148 (MQ=255)
                                                                                                            tCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGt                                    <  1:1591567/148‑1 (MQ=255)
                                                                                                             cTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGtt                                   <  2:2999352/148‑1 (MQ=255)
                                                                                                              tGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTc                                  <  1:226643/148‑1 (MQ=255)
                                                                                                               gCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCa                                 <  1:3054682/148‑1 (MQ=255)
                                                                                                                cTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCAt                                <  1:2619328/148‑1 (MQ=255)
                                                                                                                cTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCAt                                >  2:2270960/1‑148 (MQ=255)
                                                                                                                     cGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCa                           <  2:1034698/148‑1 (MQ=255)
                                                                                                                      gTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCAt                          <  2:1712827/148‑1 (MQ=255)
                                                                                                                      gTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCAt                          <  2:2770713/148‑1 (MQ=255)
                                                                                                                        gCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTa                        <  1:800724/148‑1 (MQ=255)
                                                                                                                        gCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTa                        <  1:2097195/148‑1 (MQ=255)
                                                                                                                         cTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTaa                       <  1:1184005/148‑1 (MQ=255)
                                                                                                                         cTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTaa                       <  1:2816984/148‑1 (MQ=255)
                                                                                                                           ggAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAAcc                     >  1:184658/1‑148 (MQ=255)
                                                                                                                               gCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGt                 <  2:3364608/148‑1 (MQ=255)
                                                                                                                               gCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGt                 <  2:3094951/148‑1 (MQ=255)
                                                                                                                                cAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGtt                >  1:1070166/1‑148 (MQ=255)
                                                                                                                                   tCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTaa                       >  1:2388875/1‑138 (MQ=255)
                                                                                                                                   tCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTaa                       <  2:2388875/138‑1 (MQ=255)
                                                                                                                                   tCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTgc             >  1:921374/1‑148 (MQ=255)
                                                                                                                                         atatCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCaaaa       >  1:285313/1‑148 (MQ=255)
                                                                                                                                           atCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaata     <  2:2832106/148‑1 (MQ=255)
                                                                                                                                              aCCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATg  <  1:3228382/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATG  >  CP009273/3018399‑3018688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑