Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,024,081 A→C 100% V70V (GTT→GTG)  ygfT ← putative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,024,0810AC99.1% 888.8 / ‑3.9 220V70V (GTT→GTG) ygfTputative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (134/84);  total (135/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

GCTGGTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCA  >  CP009273/3023939‑3024225
                                                                                                                                              |                                                                                                                                                
    gTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACaa                                                                                                                                         <  1:245455/148‑1 (MQ=255)
     ttACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAg                                                                                                                                        <  1:1658428/148‑1 (MQ=255)
      tACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGc                                                                                                                                       >  2:1060046/1‑148 (MQ=255)
         aaaGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGt                                                                                                                                    >  1:2760622/1‑148 (MQ=255)
         aaaGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGt                                                                                                                                    >  2:2621410/1‑148 (MQ=255)
          aaGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTa                                                                                                                                   >  1:1338862/1‑148 (MQ=255)
            ggTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAAc                                                                                                                                 >  1:294330/1‑148 (MQ=255)
               cGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCa                                                                                                                              <  1:149343/148‑1 (MQ=255)
                 cATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAg                                                                                                                            >  2:190274/1‑148 (MQ=255)
                   tttCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAggg                                                                                                                          >  2:2775778/1‑148 (MQ=255)
                    ttCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAgggg                                                                                                                         >  1:3514263/1‑148 (MQ=255)
                     tCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGc                                                                                                                        <  2:766069/148‑1 (MQ=255)
                         cgcAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCAttg                                                                                                                    <  1:3398992/148‑1 (MQ=255)
                              tCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCa                                                                                                               >  1:2358896/1‑148 (MQ=255)
                              tCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCa                                                                                                               >  1:1743092/1‑148 (MQ=255)
                               cGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCACGGGGCATTGTTGCAg                                                                                                              >  2:981576/1‑148 (MQ=255)
                               cGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAg                                                                                                              >  1:3360469/1‑148 (MQ=255)
                               cGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAg                                                                                                              >  1:2525379/1‑148 (MQ=255)
                                  aTCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGa                                                                                                           <  2:3360469/148‑1 (MQ=255)
                                  aTCGACCATCTAAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTAGAACCAATACATTTTTTCTCGTCCAGTTGTACGCTATCGGACTGGTAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGa                                                                                                           <  2:3192469/148‑1 (MQ=255)
                                   tCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGAt                                                                                                          <  2:928222/148‑1 (MQ=255)
                                       ccATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGACAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCa                                                                                                      >  2:581573/1‑148 (MQ=255)
                                        cATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAg                                                                                                     >  1:553/1‑148 (MQ=255)
                                        cATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAg                                                                                                     >  1:2243837/1‑148 (MQ=255)
                                        cATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAg                                                                                                     >  1:611990/1‑148 (MQ=255)
                                         aTCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAgg                                                                                                    >  1:2114124/1‑148 (MQ=255)
                                          tctcAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACa                                                                                                                                          <  2:871693/109‑1 (MQ=255)
                                          tctcAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGc                                                                                                   >  2:2246475/1‑148 (MQ=255)
                                          tctcAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGc                                                                                                   <  1:2747839/148‑1 (MQ=255)
                                           ctcAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGcc                                                                                                  >  2:1112200/1‑148 (MQ=255)
                                           ctcAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGcc                                                                                                  >  1:1444775/1‑148 (MQ=255)
                                              aaCGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCAcc                                                                                               >  2:2607449/1‑148 (MQ=255)
                                               acgacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCg                                                                                              >  1:880021/1‑148 (MQ=255)
                                                cgacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCgg                                                                                             >  2:440929/1‑148 (MQ=255)
                                                 gacgCCTAAGGGGCAAGCGTTTGCGCATCTTTTTCAACCAATACCTTTGTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTACCGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGa                                                                                            <  1:3219933/148‑1 (MQ=255)
                                                 gacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGa                                                                                            <  1:869290/148‑1 (MQ=255)
                                                 gacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGa                                                                                            <  1:1781780/148‑1 (MQ=255)
                                                   cgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGAtt                                                                                          <  1:3248356/148‑1 (MQ=255)
                                                        aaGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAg                                                                                     >  2:2787800/1‑148 (MQ=255)
                                                        aaGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAg                                                                                     >  1:3452884/1‑148 (MQ=255)
                                                         aGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGc                                                                                    >  2:1177605/1‑148 (MQ=255)
                                                         aGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGc                                                                                    <  1:465241/148‑1 (MQ=255)
                                                          ggggCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGAGGGCAGGCCACCGGATTCGCAGcc                                                                                   >  1:2929336/1‑148 (MQ=255)
                                                          ggggCAAGCGATTGCACATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGcc                                                                                   >  1:704962/1‑148 (MQ=255)
                                                               aaGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGcc                                                                              <  2:1269730/148‑1 (MQ=255)
                                                                aGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCt                                                                             <  1:1060046/148‑1 (MQ=255)
                                                                 gCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCtt                                                                            >  1:1777766/1‑148 (MQ=255)
                                                                 gCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCtt                                                                            >  2:3028249/1‑148 (MQ=255)
                                                                  cGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCttt                                                                           <  1:1942099/148‑1 (MQ=255)
                                                                  cGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCttt                                                                           <  2:2088962/148‑1 (MQ=255)
                                                                      tGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCt                                                                       >  2:2075045/1‑148 (MQ=255)
                                                                      tGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCt                                                                       >  1:505820/1‑148 (MQ=255)
                                                                        cgcATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTAc                                                                     >  2:2932928/1‑148 (MQ=255)
                                                                        cgcATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTAc                                                                     >  2:2933536/1‑148 (MQ=255)
                                                                            tCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACg                                                                 >  2:3278409/1‑148 (MQ=255)
                                                                            tCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACg                                                                 >  2:503301/1‑148 (MQ=255)
                                                                            tCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACg                                                                 >  2:503426/1‑148 (MQ=255)
                                                                             cTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGt                                                                <  1:1376055/148‑1 (MQ=255)
                                                                              ttttACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTg                                                               <  1:2016771/148‑1 (MQ=255)
                                                                               tttACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTgg                                                              >  1:3317005/1‑148 (MQ=255)
                                                                                 tACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGAt                                                            <  1:1827983/148‑1 (MQ=255)
                                                                                   cAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATAc                                                          >  2:247723/1‑148 (MQ=255)
                                                                                   cAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATAc                                                          >  2:1989837/1‑148 (MQ=255)
                                                                                     aCCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATAcgc                                                        >  1:1641630/1‑148 (MQ=255)
                                                                                      ccAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATAcgcg                                                       >  1:1148135/1‑148 (MQ=255)
                                                                                         aTACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTc                                                    >  1:145217/1‑148 (MQ=255)
                                                                                            cATTTTTGCTCGTCCAGTTGTACGCGATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGaa                                                 <  1:2655123/148‑1 (MQ=255)
                                                                                             aTTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGaaa                                                <  2:3452159/148‑1 (MQ=255)
                                                                                             aTTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGaaa                                                <  1:247723/148‑1 (MQ=255)
                                                                                              tttttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAg                                               <  2:1492128/148‑1 (MQ=255)
                                                                                              tttttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAg                                               >  2:1466059/1‑148 (MQ=255)
                                                                                              tttttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAg                                               >  2:526188/1‑148 (MQ=255)
                                                                                                 ttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCa                                            >  2:824115/1‑148 (MQ=255)
                                                                                                      cGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACtgtg                                       >  1:1594221/1‑148 (MQ=255)
                                                                                                      cGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACtgtg                                       <  2:1743092/148‑1 (MQ=255)
                                                                                                         ccAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACt                                    >  2:666242/1‑148 (MQ=255)
                                                                                                          cAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTc                                   <  2:2991858/148‑1 (MQ=255)
                                                                                                           aGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCa                                  >  1:12104/1‑148 (MQ=255)
                                                                                                           aGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCa                                  >  1:12589/1‑148 (MQ=255)
                                                                                                             ttGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGc                                >  1:1187999/1‑148 (MQ=255)
                                                                                                                     tATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGtt                        >  1:823891/1‑148 (MQ=255)
                                                                                                                      aTCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGttt                       >  1:2701908/1‑148 (MQ=255)
                                                                                                                       tCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGtttt                      >  1:1992489/1‑148 (MQ=255)
                                                                                                                        cGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTc                     >  2:344304/1‑148 (MQ=255)
                                                                                                                         ggACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCt                    >  1:1895000/1‑148 (MQ=255)
                                                                                                                          gACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCtt                   >  1:1170845/1‑148 (MQ=255)
                                                                                                                           aCTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTg                  <  2:1474615/148‑1 (MQ=255)
                                                                                                                           aCTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTg                  >  2:2372039/1‑148 (MQ=255)
                                                                                                                              ggAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGAtt               <  1:312532/148‑1 (MQ=255)
                                                                                                                              ggAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGAtt               >  2:1352491/1‑148 (MQ=255)
                                                                                                                               gAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtg              >  2:2550630/1‑148 (MQ=255)
                                                                                                                                 aaGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtgtg            <  2:3514263/148‑1 (MQ=255)
                                                                                                                                 aaGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtgtg            <  1:2775842/148‑1 (MQ=255)
                                                                                                                                  aGTCAGAGCATTCACCAGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtgtgt           >  2:2868812/1‑148 (MQ=255)
                                                                                                                                    tCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGc         >  2:2490403/1‑148 (MQ=255)
                                                                                                                                    tCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGc         >  1:730464/1‑148 (MQ=255)
                                                                                                                                    tCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGc         >  1:216476/1‑148 (MQ=255)
                                                                                                                                    tCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGc         >  1:3444548/1‑148 (MQ=255)
                                                                                                                                           aTTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGca  >  1:1324158/1‑148 (MQ=255)
                                                                                                                                           aTTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGca  <  2:3013413/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
GCTGGTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCA  >  CP009273/3023939‑3024225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑