Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,030,181 G→T 100% H429N (CAT→AAT)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,030,1810GT98.2% 510.9 / ‑0.3 165H429N (CAT→AAT) recJssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (102/60);  total (103/62)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 165 total aligned reads displayed.

TTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAA  >  CP009273/3030038‑3030324
                                                                                                                                               |                                                                                                                                               
ttCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCg                                                                                                                                             >  1:3528529/1‑148 (MQ=255)
ttCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCg                                                                                                                                             >  1:2690865/1‑148 (MQ=255)
 tCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGc                                                                                                                                            <  2:2075904/148‑1 (MQ=255)
   ggCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCa                                                                                                                                          <  2:2627761/148‑1 (MQ=255)
   ggCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCa                                                                                                                                          >  2:3357994/1‑148 (MQ=255)
    gCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCaa                                                                                                                                         >  2:216264/1‑148 (MQ=255)
        ggCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACtt                                                                                                                                     <  1:585967/148‑1 (MQ=255)
          cTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCa                                                                                                                                   >  2:1987319/1‑148 (MQ=255)
          cTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCa                                                                                                                                   <  1:1139362/148‑1 (MQ=255)
            tAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGc                                                                                                                                 <  1:1724676/148‑1 (MQ=255)
                ggACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGcatca                                                                                                                             <  1:474918/148‑1 (MQ=255)
                    cGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGcc                                                                                                                         <  2:486342/148‑1 (MQ=255)
                            aCCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTaga                                                                                                                 >  2:3167630/1‑148 (MQ=255)
                            aCCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTaga                                                                                                                 >  2:1058744/1‑148 (MQ=255)
                               aCTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtg                                                                                                              <  2:2980592/148‑1 (MQ=255)
                               aCTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtg                                                                                                              <  2:2980448/148‑1 (MQ=255)
                                      cTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTaa                                                                                                       <  1:263293/148‑1 (MQ=255)
                                       ttGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAAt                                                                                                      >  1:2201255/1‑148 (MQ=255)
                                             tAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTcc                                                                                                >  2:2336784/1‑148 (MQ=255)
                                              atcGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCa                                                                                               >  2:2914043/3‑148 (MQ=255)
                                              atcGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCa                                                                                               >  2:2914116/3‑148 (MQ=255)
                                                cGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAgc                                                                                             >  1:483866/1‑148 (MQ=255)
                                                cGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAgc                                                                                             >  1:483480/1‑148 (MQ=255)
                                                   aGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCa                                                                                          >  1:408186/1‑148 (MQ=255)
                                                   aGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCa                                                                                          >  2:858059/1‑148 (MQ=255)
                                                    gggTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCAt                                                                                         <  1:2491739/148‑1 (MQ=255)
                                                    gggTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCAt                                                                                         <  1:2491854/148‑1 (MQ=255)
                                                    gggTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCAt                                                                                         <  2:1538737/148‑1 (MQ=255)
                                                     ggTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATc                                                                                        >  2:199123/1‑148 (MQ=255)
                                                     ggTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATc                                                                                        >  1:3200583/1‑148 (MQ=255)
                                                           gCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCat                                                                                  >  2:2791899/1‑148 (MQ=255)
                                                            ccACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCata                                                                                 <  1:1184332/148‑1 (MQ=255)
                                                            ccACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCata                                                                                 <  1:2080808/148‑1 (MQ=255)
                                                            ccACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCata                                                                                 <  1:2080917/148‑1 (MQ=255)
                                                            ccACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCata                                                                                 >  1:190135/1‑148 (MQ=255)
                                                                  aGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGc                                                                           >  1:2450132/1‑148 (MQ=255)
                                                                  aGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGc                                                                           >  1:3545294/1‑148 (MQ=255)
                                                                    tAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGccc                                                                         >  1:2301429/1‑148 (MQ=255)
                                                                      aCCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCt                                                                       >  1:2120488/1‑148 (MQ=255)
                                                                       ccAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTg                                                                      >  1:164400/1‑148 (MQ=255)
                                                                          gTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAAt                                                                   <  1:562652/148‑1 (MQ=255)
                                                                            tCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATgg                                                                 <  2:640915/148‑1 (MQ=255)
                                                                            tCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATgg                                                                 <  2:3000951/148‑1 (MQ=255)
                                                                               ccAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGc                                                              <  2:2201255/148‑1 (MQ=255)
                                                                                cAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCg                                                             <  1:2810144/148‑1 (MQ=255)
                                                                                 aaaCCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCa                                                                             <  1:667375/131‑1 (MQ=255)
                                                                                 aaaCCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCa                                                                             >  2:667375/1‑131 (MQ=255)
                                                                                   aCCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGAcc                                                          >  2:40034/1‑148 (MQ=255)
                                                                                   aCCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGAcc                                                          >  2:2680876/1‑148 (MQ=255)
                                                                                    ccGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCg                                                         >  2:3489735/1‑148 (MQ=255)
                                                                                       ttgttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGaa                                                      <  1:899464/148‑1 (MQ=255)
                                                                                       ttgttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGaa                                                      >  1:2559229/1‑148 (MQ=255)
                                                                                       ttgttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGaa                                                      >  1:2559238/1‑148 (MQ=255)
                                                                                        tgttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAAc                                                     >  2:905710/1‑148 (MQ=255)
                                                                                         gttgAAAGATTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGAATTACCGCCAAACTTCAGCATCATGCCTGGGTAGAGTGTGTATAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAAcc                                                    <  1:2827016/148‑1 (MQ=255)
                                                                                         gttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCTGGGTAGTGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAAcc                                                    <  1:2827023/148‑1 (MQ=255)
                                                                                         gttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAAcc                                                    >  2:2841140/1‑148 (MQ=255)
                                                                                           tgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCtt                                                  >  2:1424149/1‑148 (MQ=255)
                                                                                              aaGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCa                                               >  2:942496/1‑148 (MQ=255)
                                                                                               agagTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAg                                              >  2:1072358/1‑148 (MQ=255)
                                                                                                   tttGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCCGCATCATGCCAGGGTAGTGTGTGTCTAATCGCTCCAGCGCATCACGCATTTGCAGCCCCTTAAGGGAGCGACCGGAACCTTTCAGCGTa                                          <  1:2139823/148‑1 (MQ=255)
                                                                                                   tttGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAAGCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTa                                          <  1:3314081/148‑1 (MQ=255)
                                                                                                    ttGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTAc                                         >  1:2890279/1‑148 (MQ=255)
                                                                                                       aaTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGt                                      <  2:392767/148‑1 (MQ=255)
                                                                                                         tttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCa                                    >  1:824735/1‑148 (MQ=255)
                                                                                                          ttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATTTGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCAc                                   <  1:692911/148‑1 (MQ=255)
                                                                                                          ttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCAc                                   >  1:2033751/1‑148 (MQ=255)
                                                                                                          ttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCAc                                   <  2:2875729/148‑1 (MQ=255)
                                                                                                              ccTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgc                               <  2:2323539/148‑1 (MQ=255)
                                                                                                              ccTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgc                               <  2:2323853/148‑1 (MQ=255)
                                                                                                              ccTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgc                               >  1:2011003/1‑148 (MQ=255)
                                                                                                              ccTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgc                               >  2:1560354/1‑148 (MQ=255)
                                                                                                              ccTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgc                               >  2:802118/1‑148 (MQ=255)
                                                                                                                tctTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgctg                             >  2:2592255/1‑148 (MQ=255)
                                                                                                                 ctTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTgg                            >  1:3280582/1‑148 (MQ=255)
                                                                                                                   tCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGgcg                          >  1:763688/1‑148 (MQ=255)
                                                                                                                      aGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaa                       >  1:2408219/1‑148 (MQ=255)
                                                                                                                      aGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaa                       >  1:2408269/1‑148 (MQ=255)
                                                                                                                        cGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAc                     >  2:1849521/1‑148 (MQ=255)
                                                                                                                        cGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAc                     >  2:44803/1‑148 (MQ=255)
                                                                                                                        cGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAc                     >  2:1849400/1‑148 (MQ=255)
                                                                                                                        cGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAc                     >  2:2440599/1‑148 (MQ=255)
                                                                                                                         gACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcg                    <  2:1224795/148‑1 (MQ=255)
                                                                                                                          aCAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgc                   >  2:2764097/1‑148 (MQ=255)
                                                                                                                           cAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                  >  2:2299814/1‑148 (MQ=255)
                                                                                                                              aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGGCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATa               >  1:3240443/1‑148 (MQ=255)
                                                                                                                              aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATa               >  1:280144/1‑148 (MQ=255)
                                                                                                                              aCCAGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATa               <  2:3226138/148‑1 (MQ=255)
                                                                                                                               cccGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATaa              <  2:1603033/148‑1 (MQ=255)
                                                                                                                                ccgccgCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAAc             <  1:1981523/148‑1 (MQ=255)
                                                                                                                                ccgccgCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAAc             <  1:180089/148‑1 (MQ=255)
                                                                                                                                ccgccgCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGAACCGTCACCTGCTGGCGCAAACGCGATAAc             >  1:2031310/1‑148 (MQ=255)
                                                                                                                                      ccATCGCATTACCGCCAACCTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACg       <  2:2459207/148‑1 (MQ=255)
                                                                                                                                       cATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACgg      <  1:3486819/148‑1 (MQ=255)
                                                                                                                                       cATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACgg      <  1:840346/148‑1 (MQ=255)
                                                                                                                                        aTCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTCCCGTCACCTGCGGGCGCACACGCGATAACCGGACGGt     >  2:3455779/1‑148 (MQ=255)
                                                                                                                                         tCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTg    >  1:2074195/1‑148 (MQ=255)
                                                                                                                                         tCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCCGCTGGCGCAAACGCGATAACCGGACGGTg    >  2:1704663/1‑148 (MQ=255)
                                                                                                                                          cGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGa   >  1:3102361/1‑148 (MQ=255)
                                                                                                                                           gCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaa  <  2:1403340/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAA  >  CP009273/3030038‑3030324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑