Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,039,703 C→A 100% A900S (GCG→TCG)  gcvP ← glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,039,7030CA97.2% 489.1 / 4.2 159A900S (GCG→TCG) gcvPglycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (68/86);  total (70/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 163 total aligned reads displayed.

AGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGC  >  CP009273/3039559‑3039847
                                                                                                                                                |                                                                                                                                                
aGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGtt                                                                                                                                               <  2:3384737/148‑1 (MQ=255)
  aaCAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCa                                                                                                                                             >  2:1502967/1‑148 (MQ=255)
  aaCAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCa                                                                                                                                             <  2:2683531/148‑1 (MQ=255)
  aaCAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGGTCGCTCTGAATGTGCGGCGAGTTCa                                                                                                                                             >  1:585944/1‑148 (MQ=255)
       gTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGc                                                                                                                                        >  1:1739073/1‑148 (MQ=255)
        ttACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCg                                                                                                                                       <  1:1774859/148‑1 (MQ=255)
            ggTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGtt                                                                                                                                   >  1:914738/1‑148 (MQ=255)
              tCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGTGAGTTCACCAGCGGGTTAt                                                                                                                                 <  1:2937680/148‑1 (MQ=255)
              tCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTAt                                                                                                                                 <  1:333699/148‑1 (MQ=255)
               cGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCTCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATc                                                                                                                                <  2:2850640/148‑1 (MQ=255)
                gCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCt                                                                                                                               <  2:781073/148‑1 (MQ=255)
                gCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCt                                                                                                                               <  2:2567651/148‑1 (MQ=255)
                  cGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTc                                                                                                                             <  1:1845111/148‑1 (MQ=255)
                  cGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTc                                                                                                                             <  1:163722/148‑1 (MQ=255)
                    tAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCa                                                                                                                           >  1:2232750/1‑148 (MQ=255)
                     aaaCATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAg                                                                                                                          <  1:2141546/148‑1 (MQ=255)
                       aCATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCg                                                                                                                        >  2:2767296/1‑148 (MQ=255)
                        catcatCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCgg                                                                                                                       >  1:563774/1‑148 (MQ=255)
                           catcCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCa                                                                                                                    >  1:2516212/1‑148 (MQ=255)
                            atcCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAg                                                                                                                   >  2:3441712/1‑148 (MQ=255)
                            atcCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAg                                                                                                                   >  2:2297770/1‑148 (MQ=255)
                            atcCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAg                                                                                                                   >  1:509922/1‑148 (MQ=255)
                              ccAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGac                                                                                                                 >  2:375447/1‑148 (MQ=255)
                              ccAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGac                                                                                                                 >  2:375518/1‑148 (MQ=255)
                               cAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGaca                                                                                                                <  2:2327593/148‑1 (MQ=255)
                                     tttCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCt                                                                                                          <  2:973793/148‑1 (MQ=255)
                                      ttCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCtt                                                                                                         >  2:603192/1‑148 (MQ=255)
                                        cACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCGTTc                                                                                                       >  2:3100007/1‑148 (MQ=255)
                                            gTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCt                                                                                                   <  1:202022/148‑1 (MQ=255)
                                              cGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTgg                                                                                                 <  2:2153991/148‑1 (MQ=255)
                                                   aGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAAt                                                                                            <  2:1814339/148‑1 (MQ=255)
                                                      aTTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTc                                                                                         >  1:2792090/1‑148 (MQ=255)
                                                      aTTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTc                                                                                         >  2:1104419/1‑148 (MQ=255)
                                                       tttGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCt                                                                                        >  1:560276/1‑148 (MQ=255)
                                                        ttGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTg                                                                                       >  2:1222478/1‑148 (MQ=255)
                                                           tCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTgcgc                                                                                    >  2:2420100/1‑148 (MQ=255)
                                                             tGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCgg                                                                                  <  2:312535/148‑1 (MQ=255)
                                                             tGCCACACCTGCCGGGAAGACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCgg                                                                                  <  2:2160575/148‑1 (MQ=255)
                                                               ccACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGAt                                                                                >  1:435315/1‑148 (MQ=255)
                                                               ccACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTGTCACCTGGTCAATTTCTGCGCGGAt                                                                                >  1:2919160/1‑148 (MQ=255)
                                                                 acacCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAg                                                                              <  2:2631139/148‑1 (MQ=255)
                                                                 acacCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAg                                                                              <  2:2232750/148‑1 (MQ=255)
                                                                  cacCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGc                                                                             <  2:1081640/148‑1 (MQ=255)
                                                                  cacCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGc                                                                             <  1:2497939/148‑1 (MQ=255)
                                                                    ccTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCa                                                                           >  2:400914/1‑148 (MQ=255)
                                                                      tGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGc                                                                         <  1:1146178/148‑1 (MQ=255)
                                                                       gCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCa                                                                        <  2:2521645/148‑1 (MQ=255)
                                                                       gCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCa                                                                        <  2:563774/148‑1 (MQ=255)
                                                                           ggAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATcgc                                                                    >  1:3530465/1‑148 (MQ=255)
                                                                             aagaCCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGTGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGt                                                                  <  2:2704132/145‑1 (MQ=255)
                                                                                aCCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGa                                                               >  1:1367701/1‑148 (MQ=255)
                                                                                aCCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGa                                                               <  1:1420300/148‑1 (MQ=255)
                                                                                   gCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATaa                                                            <  2:1363384/148‑1 (MQ=255)
                                                                                   gCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATaa                                                            <  1:2448064/148‑1 (MQ=255)
                                                                                    cAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATaaa                                                           >  2:1754853/1‑148 (MQ=255)
                                                                                    cAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATaaa                                                           <  2:1325728/148‑1 (MQ=255)
                                                                                     aaCTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCTAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAg                                                          <  2:2480783/148‑1 (MQ=255)
                                                                                      aCTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGc                                                         >  2:2145334/1‑148 (MQ=255)
                                                                                       cTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg                                                        <  1:3383697/148‑1 (MQ=255)
                                                                                        ttCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGa                                                       <  2:364871/148‑1 (MQ=255)
                                                                                         tCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGAt                                                      >  1:1003231/1‑148 (MQ=255)
                                                                                          cACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATc                                                     <  1:1468702/148‑1 (MQ=255)
                                                                                            cGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCa                                                   >  2:3192794/1‑148 (MQ=255)
                                                                                             ggCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAg                                                  >  1:1357768/1‑148 (MQ=255)
                                                                                                tGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTc                                               <  1:1974886/148‑1 (MQ=255)
                                                                                                  tACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCa                                             >  2:1494134/1‑148 (MQ=255)
                                                                                                  tACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCa                                             <  2:2894911/148‑1 (MQ=255)
                                                                                                    cGGATGCGCCCACTCGGCGACCAGTTCTCTCTGAATGTGCGGCGAGTTCACCAGCTGGTTCTCTTCCAGCGGCCAGACACCGGCTTTCAGCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACt                                           <  1:701919/148‑1 (MQ=255)
                                                                                                    cGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACt                                           <  1:701964/148‑1 (MQ=255)
                                                                                                    cGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACt                                           <  1:38256/148‑1 (MQ=255)
                                                                                                      gATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACttt                                         >  1:2256837/1‑148 (MQ=255)
                                                                                                       aTGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTg                                        <  1:2335775/148‑1 (MQ=255)
                                                                                                       aTGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTg                                        >  2:408392/1‑148 (MQ=255)
                                                                                                       aTGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTg                                        <  1:2898903/148‑1 (MQ=255)
                                                                                                       aTGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTg                                        <  1:2718792/148‑1 (MQ=255)
                                                                                                        tGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGc                                       <  2:2516212/148‑1 (MQ=255)
                                                                                                         gcgcCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCt                                      <  1:1398298/148‑1 (MQ=255)
                                                                                                         gcgcCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCt                                      <  1:2502245/148‑1 (MQ=255)
                                                                                                           gcCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCttt                                    <  1:912182/148‑1 (MQ=255)
                                                                                                            cccACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTc                                   >  2:559253/1‑148 (MQ=255)
                                                                                                             ccACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCa                                  >  2:2531667/1‑148 (MQ=255)
                                                                                                                  cGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTc                             <  2:1842766/148‑1 (MQ=255)
                                                                                                                  cGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTc                             <  2:1739073/148‑1 (MQ=255)
                                                                                                                  cGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTc                             <  2:2542671/148‑1 (MQ=255)
                                                                                                                    gCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAg                           <  2:1609997/148‑1 (MQ=255)
                                                                                                                       aCCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCg                        <  2:435315/148‑1 (MQ=255)
                                                                                                                         cAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGt                      >  2:3472262/1‑148 (MQ=255)
                                                                                                                           gTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTc                    >  2:2423477/1‑148 (MQ=255)
                                                                                                                            ttCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCa                   >  2:72094/1‑148 (MQ=255)
                                                                                                                             tCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCaa                  >  2:2765219/1‑148 (MQ=255)
                                                                                                                                ctctGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCa               >  1:1051618/1‑148 (MQ=255)
                                                                                                                                 tctGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt              <  1:1001667/148‑1 (MQ=255)
                                                                                                                                 tctGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt              <  2:1379602/148‑1 (MQ=255)
                                                                                                                                    gAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAg           >  1:477632/1‑148 (MQ=255)
                                                                                                                                        gtgCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTa       <  2:2758988/148‑1 (MQ=255)
                                                                                                                                         tgCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTAc      <  1:794358/148‑1 (MQ=255)
                                                                                                                                           cggcgAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCCGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTAccc    >  1:1049768/1‑148 (MQ=255)
                                                                                                                                             gcgaGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGc  <  1:400914/148‑1 (MQ=255)
                                                                                                                                             gcgaGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGc  <  2:3133408/148‑1 (MQ=255)
                                                                                                                                             gcgaGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGc  >  2:3168393/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGC  >  CP009273/3039559‑3039847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑