Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,058,769 C→T 100% I203I (ATC→ATT)  scpC → propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,058,7690CT95.3% 678.6 / 18.5 214I203I (ATC→ATT) scpCpropionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (3/7);  new base T (106/97);  total (110/104)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 214 total aligned reads displayed.

GAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCG  >  CP009273/3058625‑3058913
                                                                                                                                                |                                                                                                                                                
gaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGAt                                                                                                                                               >  1:3442617/1‑148 (MQ=255)
gaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGAt                                                                                                                                               >  2:2610780/1‑148 (MQ=255)
  aaGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATcc                                                                                                                                             >  2:2438463/1‑148 (MQ=255)
   aGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCg                                                                                                                                            >  2:2369982/1‑148 (MQ=255)
       aTCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGaaaa                                                                                                                                        <  1:2343472/148‑1 (MQ=255)
       aTCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGaaaa                                                                                                                                        >  1:1561387/1‑148 (MQ=255)
       aTCATTGAACTAAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGaaaa                                                                                                                                        <  1:8036/148‑1 (MQ=255)
         cATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAg                                                                                                                                      <  1:1283924/148‑1 (MQ=255)
           ttGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAt                                                                                                                                    >  1:2888971/1‑148 (MQ=255)
           ttGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAt                                                                                                                                    <  2:1661646/148‑1 (MQ=255)
           ttGAACTCAATCACTATCAAGATCAGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCAACCGCGGCGCAATAGCGTGTCGATCTTCAATGCAATGGATCGAGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAt                                                                                                                                    >  2:2242080/1‑148 (MQ=255)
            tGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAtt                                                                                                                                   >  1:1496085/1‑148 (MQ=255)
                cTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATACAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCg                                                                                                                               >  2:1238531/1‑148 (MQ=255)
                cTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCg                                                                                                                               <  1:2409745/148‑1 (MQ=255)
                  cAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGcc                                                                                                                             >  2:679600/1‑148 (MQ=255)
                    aTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgt                                                                                                                           >  2:614151/1‑148 (MQ=255)
                    aTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgt                                                                                                                           <  1:643414/148‑1 (MQ=255)
                        cTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTg                                                                                                                       >  1:2023003/1‑148 (MQ=255)
                        cTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTg                                                                                                                       >  1:2023255/1‑148 (MQ=255)
                         taaaaataTCCGCGCGTTGCATAACTCGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTACCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGCTCCGAAAAAGATTGTCTCCGGCGTgg                                                                                                                      >  2:2802531/8‑148 (MQ=255)
                          aTCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGa                                                                                                                     >  2:3060591/1‑148 (MQ=255)
                          aTCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGa                                                                                                                     >  2:2131125/1‑148 (MQ=255)
                           tCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGaa                                                                                                                    >  1:2430458/1‑148 (MQ=255)
                            cACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGaaa                                                                                                                   >  1:2611014/1‑148 (MQ=255)
                               gATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCa                                                                                                                <  1:3385899/148‑1 (MQ=255)
                                 tCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAAc                                                                                                              <  2:1367052/148‑1 (MQ=255)
                                 tCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAAc                                                                                                              >  1:2307651/1‑148 (MQ=255)
                                    gcgcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTg                                                                                                           <  1:1493223/148‑1 (MQ=255)
                                    gcgcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTg                                                                                                           <  1:2943775/148‑1 (MQ=255)
                                    gcgcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTg                                                                                                           <  1:1165811/148‑1 (MQ=255)
                                     cgcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGc                                                                                                          <  2:3384266/148‑1 (MQ=255)
                                     cgcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGc                                                                                                          <  2:3488424/148‑1 (MQ=255)
                                     cgcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGc                                                                                                          <  1:3560921/148‑1 (MQ=255)
                                         ttGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGa                                                                                                      >  1:164244/1‑148 (MQ=255)
                                              gAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCg                                                                                                 >  2:2720866/1‑148 (MQ=255)
                                                aCTGGCGGATAGTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGt                                                                                               <  2:1926993/148‑1 (MQ=255)
                                                 cTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATACAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTa                                                                                              <  1:1238479/148‑1 (MQ=255)
                                                  tGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTaa                                                                                             <  1:3459725/148‑1 (MQ=255)
                                                       gATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGc                                                                                        >  2:1192907/1‑148 (MQ=255)
                                                         tatTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTg                                                                                      <  1:10883/148‑1 (MQ=255)
                                                          atTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTgg                                                                                     >  1:2713692/1‑148 (MQ=255)
                                                          atTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTgg                                                                                     <  2:712407/148‑1 (MQ=255)
                                                            tgtgATTCCTGGCGCGCCCCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGAt                                                                                   >  1:2591273/1‑148 (MQ=255)
                                                             gtgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATa                                                                                  <  2:3018130/148‑1 (MQ=255)
                                                             gtgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATa                                                                                  <  1:1011392/148‑1 (MQ=255)
                                                             gtgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATa                                                                                  <  1:1010977/148‑1 (MQ=255)
                                                              tgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATaa                                                                                 <  2:1944066/148‑1 (MQ=255)
                                                               gATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAg                                                                                <  1:3073599/148‑1 (MQ=255)
                                                                aTTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGc                                                                               >  2:790691/1‑148 (MQ=255)
                                                                aTTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGc                                                                               >  2:1268192/1‑148 (MQ=255)
                                                                        cgcgcCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATccc                                                                       <  1:660205/148‑1 (MQ=255)
                                                                              aCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGc                                                                 <  2:2381805/148‑1 (MQ=255)
                                                                              aCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGc                                                                 <  1:1027254/148‑1 (MQ=255)
                                                                              aCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGc                                                                 <  2:2023020/148‑1 (MQ=255)
                                                                                     cgcAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGa                                                          >  2:36673/1‑148 (MQ=255)
                                                                                       cAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGAtt                                                        >  1:938799/1‑148 (MQ=255)
                                                                                        aaTAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTg                                                       <  2:2046050/148‑1 (MQ=255)
                                                                                         aTAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGc                                                      >  2:1006733/1‑148 (MQ=255)
                                                                                          tAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGcc                                                     <  1:679740/148‑1 (MQ=255)
                                                                                           aGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCg                                                    >  1:2698031/1‑148 (MQ=255)
                                                                                           aGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCg                                                    <  1:1728731/148‑1 (MQ=255)
                                                                                             cGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGAt                                                  >  1:1153881/1‑148 (MQ=255)
                                                                                              gtgtCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATa                                                 <  1:1606865/148‑1 (MQ=255)
                                                                                                gtCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAAc                                               >  1:875066/1‑148 (MQ=255)
                                                                                                  cGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGt                                             <  2:3442617/148‑1 (MQ=255)
                                                                                                  cGATCTTCCATGCAATCGATCGCGTCGGTACCCGCTATGGCCAAAGTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCAAGCAGATTGAAGATAACGt                                             >  1:1501811/1‑148 (MQ=255)
                                                                                                    aTCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTgg                                           >  1:1380372/1‑148 (MQ=255)
                                                                                                    aTCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTgg                                           >  1:3014747/1‑148 (MQ=255)
                                                                                                    aTCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTgg                                           <  2:2221598/148‑1 (MQ=255)
                                                                                                      cTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTc                                         <  2:891951/148‑1 (MQ=255)
                                                                                                      cTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTc                                         >  2:1257512/1‑148 (MQ=255)
                                                                                                           aTGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGtt                                    <  2:47328/148‑1 (MQ=255)
                                                                                                           aTGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCAAGCAGATTGCCGATAACGTGTTCACGtt                                    >  1:2225087/1‑148 (MQ=255)
                                                                                                            tGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTc                                   >  2:251979/1‑148 (MQ=255)
                                                                                                            tGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTc                                   <  2:534680/148‑1 (MQ=255)
                                                                                                                aTGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTAt                               >  1:551024/1‑148 (MQ=255)
                                                                                                                   gATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGc                            <  1:2852461/148‑1 (MQ=255)
                                                                                                                   gATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGc                            <  2:2879211/148‑1 (MQ=255)
                                                                                                                    aTCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCa                           <  1:565094/148‑1 (MQ=255)
                                                                                                                        cgTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGaa                       >  2:1483780/1‑148 (MQ=255)
                                                                                                                          tCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAAt                     >  2:126605/1‑148 (MQ=255)
                                                                                                                              tACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGgcg                 >  1:2543715/1‑148 (MQ=255)
                                                                                                                               aCCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGgcgc                >  2:130517/1‑148 (MQ=255)
                                                                                                                                cccGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCa               >  2:2427431/1‑148 (MQ=255)
                                                                                                                                 ccGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCAt              <  1:2727925/148‑1 (MQ=255)
                                                                                                                                   gCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATgg            <  1:596427/148‑1 (MQ=255)
                                                                                                                                   gCTATGTGCAAATTGATCCGAAAAAGAGTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATgg            <  1:2887192/148‑1 (MQ=255)
                                                                                                                                    cTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATggg           <  2:3381906/148‑1 (MQ=255)
                                                                                                                                     tATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGc          >  1:2190962/1‑148 (MQ=255)
                                                                                                                                      aTGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCg         >  2:920475/1‑148 (MQ=255)
                                                                                                                                      aTGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCg         >  2:3187652/1‑148 (MQ=255)
                                                                                                                                      aTGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCg         >  2:1369378/1‑148 (MQ=255)
                                                                                                                                        gtgCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTa       >  2:3534018/1‑148 (MQ=255)
                                                                                                                                        gtgCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTa       >  1:324223/1‑148 (MQ=255)
                                                                                                                                        gtgCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTa       <  1:2100374/148‑1 (MQ=255)
                                                                                                                                         tgCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTAt      >  2:1191611/1‑148 (MQ=255)
                                                                                                                                          gCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTAtt     >  2:1731963/1‑148 (MQ=255)
                                                                                                                                          gCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTAtt     >  1:2627057/1‑148 (MQ=255)
                                                                                                                                            aaaTTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTcc   >  1:722872/1‑148 (MQ=255)
                                                                                                                                            aaaTTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTcc   >  1:3173359/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCG  >  CP009273/3058625‑3058913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑