Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,065,123 C→T 100% G287S (GGT→AGT)  pgk ← phosphoglycerate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,065,1230CT95.5% 561.7 / 13.6 176G287S (GGT→AGT) pgkphosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base C (3/5);  new base T (86/82);  total (89/87)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 176 total aligned reads displayed.

ACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAAC  >  CP009273/3064979‑3065267
                                                                                                                                                |                                                                                                                                                
aCGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGat                                                                                                                                               >  1:2791558/1‑148 (MQ=255)
  gCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGatat                                                                                                                                             <  2:2666966/148‑1 (MQ=255)
    ttCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATcc                                                                                                                                           >  1:1740816/1‑148 (MQ=255)
     tCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCa                                                                                                                                          <  2:1666792/148‑1 (MQ=255)
        cTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGa                                                                                                                                       >  2:1079250/1‑148 (MQ=255)
        cTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCATTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGa                                                                                                                                       <  1:1879840/148‑1 (MQ=255)
            cTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTg                                                                                                                                   <  2:3247313/148‑1 (MQ=255)
            cTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTg                                                                                                                                   >  2:2526874/1‑148 (MQ=255)
            cTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTg                                                                                                                                   <  2:126372/148‑1 (MQ=255)
                gATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCg                                                                                                                               <  2:596237/148‑1 (MQ=255)
                 aTAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGt                                                                                                                              <  2:2417503/148‑1 (MQ=255)
                 aTAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGt                                                                                                                              >  2:388060/1‑148 (MQ=255)
                 aTAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGt                                                                                                                              <  1:264387/148‑1 (MQ=255)
                 aTAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGt                                                                                                                              <  1:3187512/148‑1 (MQ=255)
                 aTAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGt                                                                                                                              >  2:2182173/1‑148 (MQ=255)
                 aTAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGt                                                                                                                              <  1:3072702/148‑1 (MQ=255)
                  tAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTc                                                                                                                             <  2:2347075/148‑1 (MQ=255)
                    gCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAg                                                                                                                           >  1:349839/1‑148 (MQ=255)
                     cGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGc                                                                                                                          <  2:791399/148‑1 (MQ=255)
                     cGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGc                                                                                                                          <  1:80192/148‑1 (MQ=255)
                      gTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCt                                                                                                                         <  1:1128358/148‑1 (MQ=255)
                       ttAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGCATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCtt                                                                                                                        >  2:2755081/1‑148 (MQ=255)
                        tAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCttt                                                                                                                       <  2:2102575/148‑1 (MQ=255)
                          gCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTca                                                                                                                     <  2:1311493/148‑1 (MQ=255)
                           ccACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTcac                                                                                                                    <  2:143350/148‑1 (MQ=255)
                             aCGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACAt                                                                                                                  >  2:2056834/1‑148 (MQ=255)
                                    cAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAAc                                                                                                           <  2:2108977/148‑1 (MQ=255)
                                      gTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAg                                                                                                         <  1:2930054/148‑1 (MQ=255)
                                       tACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGa                                                                                                        >  1:459603/1‑148 (MQ=255)
                                           tttGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTc                                                                                                    <  1:1262981/148‑1 (MQ=255)
                                            ttGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCa                                                                                                   >  2:1304692/1‑148 (MQ=255)
                                             tGCGGAAGTTCGGGATTTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAg                                                                                                  <  2:723566/148‑1 (MQ=255)
                                             tGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAg                                                                                                  <  1:1946219/148‑1 (MQ=255)
                                               cGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAggg                                                                                                <  1:606912/148‑1 (MQ=255)
                                                 gAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTa                                                                                              >  2:1230982/1‑148 (MQ=255)
                                                 gAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTa                                                                                              >  1:1960559/1‑148 (MQ=255)
                                                    gTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGcc                                                                                           >  2:1722860/1‑148 (MQ=255)
                                                        gggAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTg                                                                                       >  2:3153660/1‑148 (MQ=255)
                                                          gAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCa                                                                                     >  2:2668537/1‑148 (MQ=255)
                                                              tCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGttt                                                                                 >  1:2562368/1‑148 (MQ=255)
                                                                 aaCACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGAATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCag                                                                              <  1:3278422/148‑1 (MQ=255)
                                                                  acacGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCaga                                                                             >  1:1360546/1‑148 (MQ=255)
                                                                  acacGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCaga                                                                             >  1:3104189/1‑148 (MQ=255)
                                                                   cacGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCagag                                                                            <  1:1684628/148‑1 (MQ=255)
                                                                   cacGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCagag                                                                            <  1:1684630/148‑1 (MQ=255)
                                                                        cAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTc                                                                       <  1:571233/148‑1 (MQ=255)
                                                                        cAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTc                                                                       <  2:1730542/148‑1 (MQ=255)
                                                                         aaCCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCg                                                                      >  2:2519532/1‑148 (MQ=255)
                                                                         aaCCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCg                                                                      >  1:1643280/1‑148 (MQ=255)
                                                                          aCCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCgg                                                                     <  2:1606222/148‑1 (MQ=255)
                                                                          aCCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCgg                                                                     >  1:2519360/1‑148 (MQ=255)
                                                                          aCCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCgg                                                                     >  1:2519409/1‑148 (MQ=255)
                                                                           ccGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGt                                                                    >  1:2825168/1‑148 (MQ=255)
                                                                              gACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGc                                                                 <  1:1426574/148‑1 (MQ=255)
                                                                                ccGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTa                                                               <  1:1141714/148‑1 (MQ=255)
                                                                                ccGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTa                                                               <  1:3053817/148‑1 (MQ=255)
                                                                                  gTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTAcg                                                             <  2:860781/148‑1 (MQ=255)
                                                                                   ttCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTAcgc                                                            >  2:2617589/1‑148 (MQ=255)
                                                                                       acaGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAAc                                                        <  1:2056834/148‑1 (MQ=255)
                                                                                        caGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACa                                                       >  1:2974658/1‑148 (MQ=255)
                                                                                          gAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATc                                                     >  1:2108719/1‑148 (MQ=255)
                                                                                           aaTGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCa                                                    <  1:1287732/148‑1 (MQ=255)
                                                                                            aTGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAg                                                   <  2:2959275/148‑1 (MQ=255)
                                                                                            aTGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCAGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAg                                                   >  2:3415111/1‑148 (MQ=255)
                                                                                             tGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGa                                                  <  2:2791558/148‑1 (MQ=255)
                                                                                             tGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGTAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGa                                                  >  2:2294680/1‑148 (MQ=255)
                                                                                              ggTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGAc                                                 >  1:610694/1‑148 (MQ=255)
                                                                                                ttttCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACgg                                               <  2:614125/148‑1 (MQ=255)
                                                                                                     gCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCg                                          <  2:803156/148‑1 (MQ=255)
                                                                                                      cATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCgg                                         >  1:300724/1‑148 (MQ=255)
                                                                                                       aTTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCggg                                        <  1:3241915/148‑1 (MQ=255)
                                                                                                       aTTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCggg                                        <  1:1470367/148‑1 (MQ=255)
                                                                                                        ttcttcAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGa                                       >  1:2311934/1‑148 (MQ=255)
                                                                                                         tcttcAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGAt                                      >  1:2841421/1‑148 (MQ=255)
                                                                                                         tcttcAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGAt                                      >  1:1381248/1‑148 (MQ=255)
                                                                                                            tcAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGtt                                   >  2:887479/1‑148 (MQ=255)
                                                                                                            tcAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGtt                                   >  1:2408459/1‑148 (MQ=255)
                                                                                                             cAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTg                                  <  1:2525744/148‑1 (MQ=255)
                                                                                                                gATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAg                               >  1:425604/1‑148 (MQ=255)
                                                                                                                 aTTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAgg                              >  2:805881/1‑148 (MQ=255)
                                                                                                                  tttCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAggt                             >  2:132768/1‑148 (MQ=255)
                                                                                                                    tCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAggtgg                           <  1:1230982/148‑1 (MQ=255)
                                                                                                                      agcgtgTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTc                         <  2:3275023/143‑1 (MQ=255)
                                                                                                                           gTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTcagcag                    <  1:2476085/148‑1 (MQ=255)
                                                                                                                               cTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGt                <  2:2852758/148‑1 (MQ=255)
                                                                                                                                tGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGtt               <  1:3351110/148‑1 (MQ=255)
                                                                                                                                   gCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAg            <  1:2394201/148‑1 (MQ=255)
                                                                                                                                   gCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAg            <  2:2804385/148‑1 (MQ=255)
                                                                                                                                    cGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGTAGGTGGTCAGCAGACGTTTAGc           <  2:509324/148‑1 (MQ=255)
                                                                                                                                     ggAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCt          <  2:2974658/148‑1 (MQ=255)
                                                                                                                                     ggAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCt          <  1:410803/148‑1 (MQ=255)
                                                                                                                                     ggAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCt          <  2:1200023/148‑1 (MQ=255)
                                                                                                                                      gAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCtt         >  2:3352984/1‑148 (MQ=255)
                                                                                                                                      gAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCtt         >  2:3370505/1‑148 (MQ=255)
                                                                                                                                      gAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCtt         >  2:847726/1‑148 (MQ=255)
                                                                                                                                       aaGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTc        <  1:112111/148‑1 (MQ=255)
                                                                                                                                       aaGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATAGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGACCTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTAAGCAGACGTTTAGATTc        >  1:832951/1‑148 (MQ=255)
                                                                                                                                          cATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTc     >  1:3037363/1‑148 (MQ=255)
                                                                                                                                             cACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAAc  >  1:2486471/1‑148 (MQ=255)
                                                                                                                                             cACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAAc  <  1:1601416/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAAC  >  CP009273/3064979‑3065267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑