BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,086,574	T→A	49.3%	F93L (TTT→TTA)	yqgE →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,086,574	0	T	A	49.3%	10.1 / inf	215	F93L (TTT→TTA)	yqgE	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (60/49); new base A (62/44); total (122/93)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

ATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAAT  >  CP009273/3086429‑3086720

aTCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGc                                                                                                                                                  >  1:3560224/1‑148 (MQ=255)

 tctcAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCt                                                                                                                                                 >  2:2416077/1‑148 (MQ=255)

   tcAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTc                                                                                                                                               <  1:993567/148‑1 (MQ=255)

   tcAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTc                                                                                                                                               <  1:2442937/148‑1 (MQ=255)

     aaaaTTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCa                                                                                                                                             >  1:2945105/1‑148 (MQ=255)

      aaaTTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAg                                                                                                                                            >  2:3098861/1‑148 (MQ=255)

        aTTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCa                                                                                                                                          <  1:2347044/148‑1 (MQ=255)

          tGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCAtt                                                                                                                                        <  2:3045130/148‑1 (MQ=255)

            aaGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCg                                                                                                                                      >  2:1223664/1‑148 (MQ=255)

             aGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGc                                                                                                                                     >  1:1231646/1‑148 (MQ=255)

              gggATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCa                                                                                                                                    <  2:2992639/148‑1 (MQ=255)

              gggATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCa                                                                                                                                    >  2:1259825/1‑148 (MQ=255)

                  ttCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTc                                                                                                                                <  1:3268138/148‑1 (MQ=255)

                  ttCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGGTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTc                                                                                                                                <  1:475315/148‑1 (MQ=255)

                      ggAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGac                                                                                                                            >  1:1560188/1‑148 (MQ=255)

                       gAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGaca                                                                                                                           >  2:702962/1‑148 (MQ=255)

                       gAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAAACCGACTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGaca                                                                                                                           >  2:283183/1‑148 (MQ=255)

                        aaaaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGacaa                                                                                                                          >  2:1979787/1‑148 (MQ=255)

                          aaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGacaaca                                                                                                                        <  2:2285979/148‑1 (MQ=255)

                             cTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACgg                                                                                                                     >  1:2046137/1‑148 (MQ=255)

                               gAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTa                                                                                                                   <  2:2673835/148‑1 (MQ=255)

                               gAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTa                                                                                                                   <  2:3557972/148‑1 (MQ=255)

                                aaGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTaa                                                                                                                  <  2:1149338/148‑1 (MQ=255)

                                aaGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTaa                                                                                                                  <  1:2236990/148‑1 (MQ=255)

                                aaGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTaa                                                                                                                  <  2:2604500/148‑1 (MQ=255)

                                 aGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAAt                                                                                                                 >  1:2853634/1‑148 (MQ=255)

                                  gATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATg                                                                                                                >  1:34931/1‑148 (MQ=255)

                                    tCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGAc                                                                                                              >  2:537456/1‑148 (MQ=255)

                                           ggAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTcc                                                                                                       <  2:929708/148‑1 (MQ=255)

                                           ggAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTcc                                                                                                       <  1:1210328/148‑1 (MQ=255)

                                           ggAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTcc                                                                                                       <  2:3380310/148‑1 (MQ=255)

                                             aGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCcg                                                                                                     >  2:1557789/1‑148 (MQ=255)

                                               ccGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCcgcg                                                                                                   >  2:1790848/1‑148 (MQ=255)

                                                cgcgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGa                                                                                                  <  2:862574/148‑1 (MQ=255)

                                                cgcgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGa                                                                                                  <  2:2581632/148‑1 (MQ=255)

                                                  cgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGAtg                                                                                                >  1:1472356/1‑148 (MQ=255)

                                                    tgatgaATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGAtgtg                                                                                              <  1:225644/148‑1 (MQ=255)

                                                           tcaatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGaaa                                                                                       >  2:3497079/1‑148 (MQ=255)

                                                            caatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAAc                                                                                      >  2:2221698/1‑148 (MQ=255)

                                                            caatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAAc                                                                                      >  2:3521016/1‑148 (MQ=255)

                                                            caatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAAc                                                                                      >  2:2728397/1‑148 (MQ=255)

                                                             aatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACg                                                                                     <  2:638921/148‑1 (MQ=255)

                                                              atcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGc                                                                                    >  2:3186093/1‑148 (MQ=255)

                                                              atcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGc                                                                                    >  1:2161774/1‑148 (MQ=255)

                                                               tcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCt                                                                                   <  2:203236/148‑1 (MQ=255)

                                                                   tCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGc                                                                               >  1:3430120/1‑148 (MQ=255)

                                                                    cTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAGTTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCa                                                                              >  1:2102922/1‑148 (MQ=255)

                                                                    cTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCa                                                                              >  2:290149/1‑148 (MQ=255)

                                                                    cTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCa                                                                              >  1:1857409/1‑148 (MQ=255)

                                                                        aTAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGa                                                                          >  1:1902805/1‑148 (MQ=255)

                                                                        aTAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGa                                                                          <  1:1742263/148‑1 (MQ=255)

                                                                           aaCCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATaa                                                                       >  2:40196/1‑148 (MQ=255)

                                                                               ggTGATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaacaa                                                                   <  2:1101242/148‑1 (MQ=255)

                                                                                  tATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCg                                                                >  2:2226221/1‑148 (MQ=255)

                                                                                   aTGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGt                                                               <  2:2046137/148‑1 (MQ=255)

                                                                                    tGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTc                                                              <  2:1716525/148‑1 (MQ=255)

                                                                                     gCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCt                                                             >  1:2431378/1‑148 (MQ=255)

                                                                                     gCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCt                                                             >  1:641174/1‑148 (MQ=255)

                                                                                          gcggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGt                                                        <  1:2786903/148‑1 (MQ=255)

                                                                                           cggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTa                                                       >  1:2239150/1‑148 (MQ=255)

                                                                                            ggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTAt                                                      <  1:1548508/148‑1 (MQ=255)

                                                                                            ggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTAt                                                      <  2:2161774/148‑1 (MQ=255)

                                                                                               ccGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtgg                                                   <  1:1009809/148‑1 (MQ=255)

                                                                                               ccGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtgg                                                   >  1:3243025/1‑148 (MQ=255)

                                                                                                  ctggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtggtgg                                                <  1:1407569/148‑1 (MQ=255)

                                                                                                  ctggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtggtgg                                                >  2:1674860/1‑148 (MQ=255)

                                                                                                  ctggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtggtgg                                                >  2:3433818/1‑148 (MQ=255)

                                                                                                  ctggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtggtgg                                                >  2:2463690/1‑148 (MQ=255)

                                                                                                   tggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGc                                               <  2:3384437/148‑1 (MQ=255)

                                                                                                     gctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGctc                                             >  1:341931/1‑148 (MQ=255)

                                                                                                     gctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGctc                                             >  1:1689326/1‑148 (MQ=255)

                                                                                                     gctgAAGATCGCGGGCTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGctc                                             >  1:1240889/1‑148 (MQ=255)

                                                                                                       tgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTg                                           >  2:2880857/1‑148 (MQ=255)

                                                                                                        gAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTgg                                          >  1:3122956/1‑148 (MQ=255)

                                                                                                        gAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTgg                                          <  2:2061639/148‑1 (MQ=255)

                                                                                                         aaGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTggg                                         >  1:3003457/1‑148 (MQ=255)

                                                                                                         aaGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTggg                                         >  2:3211095/1‑148 (MQ=255)

                                                                                                          aGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGt                                        <  1:3079122/148‑1 (MQ=255)

                                                                                                              cgcgGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATg                                    >  1:3176640/1‑148 (MQ=255)

                                                                                                                  ggTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctc                                <  2:2953521/148‑1 (MQ=255)

                                                                                                                  ggTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctc                                <  2:2982223/148‑1 (MQ=255)

                                                                                                                  ggTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctc                                >  2:816372/1‑148 (MQ=255)

                                                                                                                   gTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcc                               <  1:2774602/148‑1 (MQ=255)

                                                                                                                   gTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcc                               <  1:2675669/148‑1 (MQ=255)

                                                                                                                   gTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcc                               >  1:3389328/1‑148 (MQ=255)

                                                                                                                    tttATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcct                              >  2:93857/1‑148 (MQ=255)

                                                                                                                    tttATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcct                              >  2:2658299/1‑148 (MQ=255)

                                                                                                                    tttATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcct                              <  1:3004405/148‑1 (MQ=255)

                                                                                                                       aTTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTggg                           >  1:886713/1‑148 (MQ=255)

                                                                                                                           tGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGaa                       <  1:2966033/148‑1 (MQ=255)

                                                                                                                              aTACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAgg                    <  1:2555447/148‑1 (MQ=255)

                                                                                                                               tACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGt                   <  1:3060514/148‑1 (MQ=255)

                                                                                                                                   ccGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAc               <  1:2280539/148‑1 (MQ=255)

                                                                                                                                   ccGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAc               <  1:1368121/148‑1 (MQ=255)

                                                                                                                                   ccGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGGAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAc               <  1:535270/148‑1 (MQ=255)

                                                                                                                                   ccGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAc               >  1:1888003/1‑148 (MQ=255)

                                                                                                                                           cAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGGCTCCTGGGAGAAAGGTCAACTGGAACaa       <  1:747805/148‑1 (MQ=255)

                                                                                                                                            aaTTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAg      <  2:3210651/148‑1 (MQ=255)

                                                                                                                                              ttttGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGaa    <  1:1110585/148‑1 (MQ=255)

                                                                                                                                              tttaGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGaa    <  2:1611556/148‑1 (MQ=255)

ATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAAT  >  CP009273/3086429‑3086720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`