Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,110,213 T→A 100% A753A (GCA→GCT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,110,2130TA97.2% 676.3 / 6.8 210A753A (GCA→GCT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base A (103/101);  total (106/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 210 total aligned reads displayed.

CTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGT  >  CP009273/3110068‑3110356
                                                                                                                                                 |                                                                                                                                               
cTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGc                                                                                                                                               <  1:1566584/148‑1 (MQ=255)
 ttCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGcc                                                                                                                                              <  1:1141185/148‑1 (MQ=255)
  tCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCa                                                                                                                                             >  1:2114367/1‑148 (MQ=255)
       tGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGAc                                                                                                                                        >  1:1703565/1‑148 (MQ=255)
         tCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAg                                                                                                                                      >  2:907995/1‑148 (MQ=255)
         tCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAg                                                                                                                                      >  2:2289234/1‑148 (MQ=255)
         tCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAg                                                                                                                                      >  1:3472412/1‑148 (MQ=255)
          cTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGa                                                                                                                                     >  2:3544779/1‑148 (MQ=255)
          cTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGa                                                                                                                                     <  1:2869796/148‑1 (MQ=255)
              cTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTg                                                                                                                                 <  2:1025823/148‑1 (MQ=255)
              cTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTg                                                                                                                                 <  1:3201914/148‑1 (MQ=255)
                 tCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCgg                                                                                                                              >  1:1839406/1‑148 (MQ=255)
                    aTGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCAt                                                                                                                           <  1:3380717/148‑1 (MQ=255)
                        tgtACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCaa                                                                                                                       <  2:2377237/148‑1 (MQ=255)
                        tgtACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCaa                                                                                                                       <  1:2322285/148‑1 (MQ=255)
                         gtACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAAc                                                                                                                      <  2:536986/148‑1 (MQ=255)
                         gtACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATACAAc                                                                                                                      <  2:682643/148‑1 (MQ=255)
                           aCGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAg                                                                                                                    <  2:1839406/148‑1 (MQ=255)
                               cAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGTTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGc                                                                                                                >  1:2506976/1‑148 (MQ=255)
                               cAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGc                                                                                                                >  2:408529/1‑148 (MQ=255)
                               cAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGc                                                                                                                >  1:606670/1‑148 (MQ=255)
                                    cACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACaaa                                                                                                           <  2:1597699/148‑1 (MQ=255)
                                      ccATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAc                                                                                                         >  1:568581/1‑148 (MQ=255)
                                       cATCTTCGGCAGGATAACGTTCTTAGTGCCATATGCCCGTTGCGCGCTGCTGTCGTACGCGGTTCGGATAGCCTAGCGGTTGGTTATTTACTTGCGACTTGTTGAGTGCCATCGACAGACGTGGGGCTTCCTTCTGTCGCTCAAAAcc                                                                                                        <  2:3407396/148‑1 (MQ=255)
                                       cATCTACGTCAGGATAACTTTCATAGAATCAAATGCACGTTGCGCGCTTCTTAAGAACGCTGTTAGGATACCCTTTAGGATCGTTATTTACTACCGACTTGTTCAGATTCATCTACAGACATGCGGCATCCAACAGACGCACATAAcc                                                                                                        <  2:2729187/148‑1 (MQ=255)
                                        aTCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCa                                                                                                       >  2:2252099/1‑148 (MQ=255)
                                         tCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAg                                                                                                      <  2:2077535/148‑1 (MQ=255)
                                         tCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAg                                                                                                      >  1:3481560/1‑148 (MQ=255)
                                         tCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAg                                                                                                      >  1:2634882/1‑148 (MQ=255)
                                          cTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGa                                                                                                     <  1:573379/148‑1 (MQ=255)
                                              ggCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcgcg                                                                                                 <  2:2031131/148‑1 (MQ=255)
                                               gCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACgcgc                                                                                                >  2:399202/1‑148 (MQ=255)
                                               gCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACgcgc                                                                                                >  2:398430/1‑148 (MQ=255)
                                                 aGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctc                                                                                              >  1:1195848/1‑148 (MQ=255)
                                                  ggATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctct                                                                                             <  1:1945953/148‑1 (MQ=255)
                                                   gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                            >  1:1529409/1‑148 (MQ=255)
                                                     tAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCtt                                                                                          >  2:733188/1‑148 (MQ=255)
                                                     tAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCtt                                                                                          <  1:3544779/148‑1 (MQ=255)
                                                      aaCGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTc                                                                                         <  1:475149/148‑1 (MQ=255)
                                                       aCGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTcc                                                                                        <  2:3130139/148‑1 (MQ=255)
                                                        cGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCt                                                                                       >  1:1574085/1‑148 (MQ=255)
                                                        cGTTCATAGACCCAAATGCCCGTTGCGCGCTGATGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTTTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCt                                                                                       <  2:1625465/148‑1 (MQ=255)
                                                           tCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTaa                                                                                    >  2:1828844/1‑148 (MQ=255)
                                                           tCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTaa                                                                                    >  2:3158003/1‑148 (MQ=255)
                                                              tAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                                                                                 <  1:210086/148‑1 (MQ=255)
                                                                gACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTg                                                                               <  2:168615/148‑1 (MQ=255)
                                                                gACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTg                                                                               <  1:3199287/148‑1 (MQ=255)
                                                                 acacAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGc                                                                              >  2:1270203/4‑148 (MQ=255)
                                                                  cccAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCt                                                                             <  1:2701483/148‑1 (MQ=255)
                                                                   ccAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtc                                                                            >  1:1728383/1‑148 (MQ=255)
                                                                    cAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtca                                                                           <  1:891969/148‑1 (MQ=255)
                                                                      aaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                         >  1:2830111/1‑148 (MQ=255)
                                                                      aaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                         >  2:2239525/1‑148 (MQ=255)
                                                                      aaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                         >  2:3056131/1‑148 (MQ=255)
                                                                        tGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCAc                                                                       >  1:2034220/1‑148 (MQ=255)
                                                                           ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGtt                                                                    >  2:2936850/1‑148 (MQ=255)
                                                                           ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGtt                                                                    >  2:814904/1‑148 (MQ=255)
                                                                           ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGtt                                                                    >  2:2809283/1‑148 (MQ=255)
                                                                           ccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGtt                                                                    >  2:2937237/1‑148 (MQ=255)
                                                                              ttGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTc                                                                 >  2:2087358/1‑148 (MQ=255)
                                                                               tGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAACCCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTcc                                                                <  2:1427115/148‑1 (MQ=255)
                                                                               tGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTcc                                                                <  1:761305/148‑1 (MQ=255)
                                                                                       gctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAg                                                        <  1:1314373/148‑1 (MQ=255)
                                                                                       gctgACGAACGCGGTTCGGATACCCGTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAg                                                        <  1:3100932/148‑1 (MQ=255)
                                                                                           aCGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAcc                                                    <  1:2463275/148‑1 (MQ=255)
                                                                                           aCGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAcc                                                    >  2:3531328/1‑148 (MQ=255)
                                                                                              aaCGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGAt                                                 <  1:2940720/148‑1 (MQ=255)
                                                                                               aCGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAACACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATc                                                <  2:753732/148‑1 (MQ=255)
                                                                                                cgcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTGTCCATGATCAGCACCGATcc                                               <  1:136707/148‑1 (MQ=255)
                                                                                                  cgGTTCGTATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACTCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCAc                                             <  2:475046/148‑1 (MQ=255)
                                                                                                  cgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCAc                                             <  1:2087337/148‑1 (MQ=255)
                                                                                                  cgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCAc                                             <  2:1131257/148‑1 (MQ=255)
                                                                                                      tCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCttt                                         >  1:1002790/1‑148 (MQ=255)
                                                                                                        ggATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGt                                       <  1:3334937/148‑1 (MQ=255)
                                                                                                         gATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                      >  1:247348/1‑148 (MQ=255)
                                                                                                         gATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                      >  1:725212/1‑148 (MQ=255)
                                                                                                          aTACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTc                                     >  2:3290730/1‑148 (MQ=255)
                                                                                                            aCCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAg                                   >  1:3347886/1‑148 (MQ=255)
                                                                                                              ccTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGAt                                 >  2:2202218/1‑148 (MQ=255)
                                                                                                                ttGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAg                               >  1:377022/1‑148 (MQ=255)
                                                                                                                 tGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAgg                              <  1:1229801/148‑1 (MQ=255)
                                                                                                                    ggATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGa                           >  1:306310/1‑148 (MQ=255)
                                                                                                                         gTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGa                      <  2:2614979/148‑1 (MQ=255)
                                                                                                                         gTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGa                      <  2:3326506/148‑1 (MQ=255)
                                                                                                                              ttACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                 >  2:210656/1‑148 (MQ=255)
                                                                                                                              ttACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                 >  2:2524018/1‑148 (MQ=255)
                                                                                                                              ttACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                 >  2:2186506/1‑148 (MQ=255)
                                                                                                                               tactacCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTc                <  2:2850401/148‑1 (MQ=255)
                                                                                                                                    ccGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATc           <  2:1107558/148‑1 (MQ=255)
                                                                                                                                    ccGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATc           >  2:3435498/1‑148 (MQ=255)
                                                                                                                                    ccGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATc           >  1:2748775/1‑148 (MQ=255)
                                                                                                                                     cGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATcc          <  2:3483797/148‑1 (MQ=255)
                                                                                                                                     cGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATcc          <  1:3125096/148‑1 (MQ=255)
                                                                                                                                       aCTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctg        <  1:1117778/148‑1 (MQ=255)
                                                                                                                                        cTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgc       >  2:2396483/1‑148 (MQ=255)
                                                                                                                                        cTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgc       >  2:1483704/1‑148 (MQ=255)
                                                                                                                                           gTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGa    >  1:2635408/1‑148 (MQ=255)
                                                                                                                                           gTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGa    >  1:2589207/1‑148 (MQ=255)
                                                                                                                                            ttCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAg   >  2:1224356/1‑148 (MQ=255)
                                                                                                                                            ttCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCNCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAg   >  1:1808969/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGT  >  CP009273/3110068‑3110356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑