Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,142,668 G→T 100% L55I (CTC→ATC)  yqhA ← UPF0114 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,142,6680GT96.9% 585.5 / 7.5 192L55I (CTC→ATC) yqhAUPF0114 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base T (105/81);  total (109/83)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 196 total aligned reads displayed.

ACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCA  >  CP009273/3142521‑3142810
                                                                                                                                                   |                                                                                                                                              
aCGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAg                                                                                                                                                >  1:3076562/1‑148 (MQ=255)
  gTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGa                                                                                                                                              <  2:2448687/148‑1 (MQ=255)
   ttGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACgatgat                                                                                                                                             <  2:3546935/148‑1 (MQ=255)
      cGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCaa                                                                                                                                          <  2:757102/148‑1 (MQ=255)
      cGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCaa                                                                                                                                          <  2:722396/148‑1 (MQ=255)
      cGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCaa                                                                                                                                          <  2:423421/148‑1 (MQ=255)
        tCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAAt                                                                                                                                        <  2:535492/148‑1 (MQ=255)
           aTTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGACAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTg                                                                                                                                     >  2:1557085/1‑148 (MQ=255)
            ttttCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGa                                                                                                                                    <  2:3307260/148‑1 (MQ=255)
                ccccAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTcc                                                                                                                                >  2:1130273/1‑148 (MQ=255)
                ccccAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTcc                                                                                                                                >  1:2461722/1‑148 (MQ=255)
                   cagccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGcc                                                                                                                             >  1:928871/1‑148 (MQ=255)
                       cagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCg                                                                                                                         >  1:86933/1‑148 (MQ=255)
                          ttCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgaga                                                                                                                      >  2:1648203/1‑148 (MQ=255)
                          ttCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgaga                                                                                                                      >  1:2357894/1‑148 (MQ=255)
                               cTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGata                                                                                                                 <  2:3126454/148‑1 (MQ=255)
                               cTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCACCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGata                                                                                                                 <  1:2141812/148‑1 (MQ=255)
                                ttCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGatat                                                                                                                >  1:762617/1‑148 (MQ=255)
                                 tctcTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATAtt                                                                                                               >  2:864999/1‑148 (MQ=255)
                                  ctctTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTc                                                                                                              <  1:890502/148‑1 (MQ=255)
                                  ctctTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTc                                                                                                              <  1:1534965/148‑1 (MQ=255)
                                   tctTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCg                                                                                                             >  1:3150416/1‑148 (MQ=255)
                                    ctTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCgg                                                                                                            >  2:318117/1‑148 (MQ=255)
                                    ctTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCgg                                                                                                            <  2:2763511/148‑1 (MQ=255)
                                     tttGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGc                                                                                                           <  1:298388/148‑1 (MQ=255)
                                     tttGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGc                                                                                                           <  1:298365/148‑1 (MQ=255)
                                      ttGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCa                                                                                                          >  2:1122946/1‑148 (MQ=255)
                                          tctcGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc                                                                                                      >  1:2827034/1‑148 (MQ=255)
                                           ctcGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACg                                                                                                     <  2:1471834/148‑1 (MQ=255)
                                             cGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTg                                                                                                   >  2:1829349/1‑148 (MQ=255)
                                               gagaTATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaa                                                                                                 <  1:3248883/148‑1 (MQ=255)
                                               gagaTATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaa                                                                                                 <  1:1476671/148‑1 (MQ=255)
                                               gagaTATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaa                                                                                                 >  2:3530742/1‑148 (MQ=255)
                                                 gaTATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaata                                                                                               <  1:819646/148‑1 (MQ=255)
                                                   tatCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaataat                                                                                             <  1:1330078/148‑1 (MQ=255)
                                                   tatCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaataat                                                                                             <  1:988345/148‑1 (MQ=255)
                                                              gagaCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaaga                                                                                  <  2:2041831/148‑1 (MQ=255)
                                                                   gAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTc                                                                             <  1:2764340/148‑1 (MQ=255)
                                                                    aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCa                                                                            <  1:1413390/148‑1 (MQ=255)
                                                                     aaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAAAACGATGATCAAATCTGATTCCGAGATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAg                                                                           <  2:1790360/148‑1 (MQ=255)
                                                                       ttCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAgcg                                                                         >  1:1980860/1‑148 (MQ=255)
                                                                        tctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAgcgc                                                                        <  1:658224/148‑1 (MQ=255)
                                                                        tctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAgcgc                                                                        >  1:1011296/1‑148 (MQ=255)
                                                                          tcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCa                                                                      >  2:697323/1‑148 (MQ=255)
                                                                            aTAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCAACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAgg                                                                    >  2:424662/1‑148 (MQ=255)
                                                                              aaCCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGc                                                                  >  1:604118/1‑148 (MQ=255)
                                                                              aaCCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGc                                                                  >  1:2501456/1‑148 (MQ=255)
                                                                                ccGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCaa                                                                >  2:1283221/1‑148 (MQ=255)
                                                                                   gAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTa                                                             >  1:1001902/1‑148 (MQ=255)
                                                                                   gAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTa                                                             <  1:2561187/148‑1 (MQ=255)
                                                                                    aaaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTaa                                                            <  2:928871/148‑1 (MQ=255)
                                                                                    aaaaCAACACCATCACCACCAAACCGCCAACCAGCGTAATACCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTaa                                                            <  1:1519723/148‑1 (MQ=255)
                                                                                      aaCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACg                                                          >  2:2137713/1‑148 (MQ=255)
                                                                                       aCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGc                                                         <  1:1484008/148‑1 (MQ=255)
                                                                                       aCATCACCATCACCAGCAAAACGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTTAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGc                                                         >  1:212042/1‑148 (MQ=255)
                                                                                        catcaccatcacCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGcc                                                        >  2:1296009/1‑148 (MQ=255)
                                                                                        catcaccatcacCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGcc                                                        >  1:2074600/1‑148 (MQ=255)
                                                                                        catcaccatcacCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGcc                                                        >  2:1173659/1‑148 (MQ=255)
                                                                                          tcaccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAg                                                      >  2:2516206/1‑148 (MQ=255)
                                                                                            accatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCg                                                    >  2:1322654/1‑148 (MQ=255)
                                                                                            accatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCg                                                    >  2:2260933/1‑148 (MQ=255)
                                                                                            accatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCg                                                    >  2:2260741/1‑148 (MQ=255)
                                                                                              catcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGaa                                                  >  1:2475972/1‑148 (MQ=255)
                                                                                              catcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGaa                                                  >  2:288168/1‑148 (MQ=255)
                                                                                              catcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGAGCCAGGGCAACTAACGCCAGCGaa                                                  >  1:690581/1‑148 (MQ=255)
                                                                                               atcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGaaa                                                 >  2:2115817/1‑148 (MQ=255)
                                                                                               atcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGaaa                                                 <  1:506925/148‑1 (MQ=255)
                                                                                                  accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGc                                              >  1:118718/1‑148 (MQ=255)
                                                                                                     aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCaa                                           >  1:3084738/1‑148 (MQ=255)
                                                                                                      gCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCaaa                                          >  1:3462166/1‑148 (MQ=255)
                                                                                                       cAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                         >  2:1485159/1‑148 (MQ=255)
                                                                                                       cAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                         >  2:797841/1‑148 (MQ=255)
                                                                                                         aaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTa                                       >  2:659820/1‑148 (MQ=255)
                                                                                                         aaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTa                                       >  1:1560995/1‑148 (MQ=255)
                                                                                                           ccGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGACAGGCCAAAGTaca                                     >  2:2087199/1‑148 (MQ=255)
                                                                                                           ccGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTaca                                     >  1:1970720/1‑148 (MQ=255)
                                                                                                           ccGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTaca                                     <  2:3446423/148‑1 (MQ=255)
                                                                                                           ccGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTaca                                     >  1:46689/1‑148 (MQ=255)
                                                                                                           ccGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTaca                                     >  2:2084971/1‑148 (MQ=255)
                                                                                                             gCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACg                                   <  1:842739/148‑1 (MQ=255)
                                                                                                                  ccAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGc                              >  2:2749529/1‑148 (MQ=255)
                                                                                                                   cAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTTCACGGGGGCa                             >  2:1286494/1‑148 (MQ=255)
                                                                                                                   cAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTTCACGGGGGCa                             >  2:3062150/1‑148 (MQ=255)
                                                                                                                   cAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCa                             <  2:1220113/148‑1 (MQ=255)
                                                                                                                      cGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagc                          >  2:272877/1‑148 (MQ=255)
                                                                                                                        tCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGTCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcag                        <  2:1706614/148‑1 (MQ=255)
                                                                                                                         cATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcagc                       <  1:2870117/148‑1 (MQ=255)
                                                                                                                             tCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagccagc                   >  2:544392/1‑148 (MQ=255)
                                                                                                                             tCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagccagc                   >  2:2744390/1‑148 (MQ=255)
                                                                                                                                 ccaGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCgaga               <  1:1617706/148‑1 (MQ=255)
                                                                                                                                 ccaGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCgaga               <  2:1295014/148‑1 (MQ=255)
                                                                                                                                  caGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGaa              >  2:2993033/1‑148 (MQ=255)
                                                                                                                                   aGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAg             <  2:835929/148‑1 (MQ=255)
                                                                                                                                    gccaCAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGc            <  1:3340458/145‑1 (MQ=255)
                                                                                                                                      gACAGCAACAAGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCAt          <  2:1523510/148‑1 (MQ=255)
                                                                                                                                         aGCAACACGTTGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCTGTACTTTAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACa       <  1:1273579/148‑1 (MQ=255)
                                                                                                                                          gCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACAt      >  1:3279166/1‑148 (MQ=255)
                                                                                                                                          gCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACAt      <  2:1611670/148‑1 (MQ=255)
                                                                                                                                             acacGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGc   >  2:1538439/1‑148 (MQ=255)
                                                                                                                                              cacGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCa  >  2:2274676/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
ACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCA  >  CP009273/3142521‑3142810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑