BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,169,188	C→A	26.3%	G60C (GGT→TGT)	yqiA ←	acyl CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,169,188	0	C	A	26.3%	375.9 / 100.7	167	G60C (GGT→TGT)	yqiA	acyl CoA esterase
Reads supporting (aligned to +/- strand): ref base C (59/64); new base A (24/20); total (83/84)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

GGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTT  >  CP009273/3169041‑3169335

ggTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCAcc                                                                                                                                                     >  1:918793/1‑148 (MQ=255)

   tctcGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGcc                                                                                                                                                  >  1:3435976/1‑148 (MQ=255)

      cGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATg                                                                                                                                               >  1:1019385/1‑148 (MQ=255)

        ttCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGtt                                                                                                                                             >  1:2767694/1‑148 (MQ=255)

         tCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTc                                                                                                                                            <  1:82148/148‑1 (MQ=255)

         tCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTc                                                                                                                                            >  1:2618271/1‑148 (MQ=255)

         tCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTc                                                                                                                                            <  1:1791054/148‑1 (MQ=255)

           tGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCa                                                                                                                                          <  2:1257746/148‑1 (MQ=255)

              ccGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGa                                                                                                                                       >  1:1138473/1‑148 (MQ=255)

                 agaTAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACaa                                                                                                                                    >  1:3296049/1‑148 (MQ=255)

                 agaTAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACaa                                                                                                                                    >  1:2152465/1‑148 (MQ=255)

                  gaTAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAAt                                                                                                                                   <  1:1842266/148‑1 (MQ=255)

                   aTAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATg                                                                                                                                  >  2:178827/1‑148 (MQ=255)

                   aTAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATg                                                                                                                                  >  1:2354484/1‑148 (MQ=255)

                      gTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGAt                                                                                                                               <  1:2458364/148‑1 (MQ=255)

                       tCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGAtt                                                                                                                              >  2:2214070/1‑148 (MQ=255)

                         cGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTcc                                                                                                                            <  2:2715029/148‑1 (MQ=255)

                         cGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTcc                                                                                                                            <  1:402467/148‑1 (MQ=255)

                         cGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTcc                                                                                                                            <  2:1800493/148‑1 (MQ=255)

                              gcagTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCcagcag                                                                                                                       >  1:2867814/1‑148 (MQ=255)

                                agTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGct                                                                                                                     <  1:2741279/148‑1 (MQ=255)

                                  ttCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGctct                                                                                                                   >  1:3526659/1‑148 (MQ=255)

                                       aCGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCcgc                                                                                                              >  2:2464131/1‑148 (MQ=255)

                                        cGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCcgcg                                                                                                             >  2:2713011/1‑148 (MQ=255)

                                        cGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCcgcg                                                                                                             >  2:2292521/1‑148 (MQ=255)

                                        cGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCcgcg                                                                                                             >  2:498924/1‑148 (MQ=255)

                                             gcACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCCGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGa                                                                                                        <  2:770317/148‑1 (MQ=255)

                                             gcACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGa                                                                                                        <  1:1983943/148‑1 (MQ=255)

                                                 cgccgGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGa                                                                                                    >  1:732266/1‑148 (MQ=255)

                                                      ggTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATAcgg                                                                                               <  2:3449517/148‑1 (MQ=255)

                                                        tttACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATAcggcg                                                                                             <  2:3294250/148‑1 (MQ=255)

                                                            ccaccaCTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCaa                                                                                         <  1:2124929/148‑1 (MQ=255)

                                                            ccaccaCTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCaa                                                                                         <  1:1560605/148‑1 (MQ=255)

                                                            ccaccaCTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTACCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCaa                                                                                         <  2:3478902/148‑1 (MQ=255)

                                                              accacTGCGGGCAGCATAAAACATTGTGACAACAAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATAACCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACt                                                                                       >  2:372700/1‑148 (MQ=255)

                                                               ccacTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTg                                                                                      <  1:826886/148‑1 (MQ=255)

                                                                cacTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGc                                                                                     >  2:1530344/1‑148 (MQ=255)

                                                                  cTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCgg                                                                                   >  2:1351977/1‑148 (MQ=255)

                                                                   tGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGa                                                                                  <  2:446142/148‑1 (MQ=255)

                                                                   tGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGa                                                                                  >  2:1252847/1‑148 (MQ=255)

                                                                   tGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGa                                                                                  <  1:1319783/148‑1 (MQ=255)

                                                                       ggCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGa                                                                              <  2:3108706/148‑1 (MQ=255)

                                                                       ggCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGa                                                                              <  1:2551386/148‑1 (MQ=255)

                                                                       ggCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGa                                                                              <  1:2551062/148‑1 (MQ=255)

                                                                       ggCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGa                                                                              >  1:3184457/1‑148 (MQ=255)

                                                                       ggCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGa                                                                              <  1:23327/148‑1 (MQ=255)

                                                                       ggCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGa                                                                              <  2:2767694/148‑1 (MQ=255)

                                                                           gcaTAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGatcat                                                                          >  1:3258983/1‑148 (MQ=255)

                                                                             aTAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCAtct                                                                        <  1:118340/148‑1 (MQ=255)

                                                                              tAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCAtctc                                                                       >  1:3518041/1‑148 (MQ=255)

                                                                              tAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCAtctc                                                                       >  1:1225300/1‑148 (MQ=255)

                                                                               aaaaCATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCa                                                                      >  2:3461310/1‑148 (MQ=255)

                                                                               aaaaCATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCa                                                                      >  2:737689/1‑148 (MQ=255)

                                                                                aaaCATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCaa                                                                     <  2:2603084/148‑1 (MQ=255)

                                                                                 aaCATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAAc                                                                    >  2:2555159/1‑148 (MQ=255)

                                                                                 aaCATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAAc                                                                    >  2:2236885/1‑148 (MQ=255)

                                                                                  aCATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCTAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACg                                                                   <  1:1878564/148‑1 (MQ=255)

                                                                                      tgtgACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAg                                                               <  1:3441813/148‑1 (MQ=255)

                                                                                        tgACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAggg                                                             <  2:1780510/148‑1 (MQ=255)

                                                                                        tgACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAggg                                                             <  1:378712/148‑1 (MQ=255)

                                                                                             aCCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATg                                                        <  2:90978/148‑1 (MQ=255)

                                                                                                aGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTc                                                     <  2:2529155/148‑1 (MQ=255)

                                                                                                aGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTc                                                     <  2:1280345/148‑1 (MQ=255)

                                                                                                  gTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCg                                                   >  2:602210/1‑148 (MQ=255)

                                                                                                  gTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCg                                                   >  1:1064811/1‑148 (MQ=255)

                                                                                                       gTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCgccagc                                              <  2:1003100/148‑1 (MQ=255)

                                                                                                          atatCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGccagccag                                           >  2:417996/1‑148 (MQ=255)

                                                                                                          atatCCCCCCAGTGACGAACCGACAAGACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGAAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGccagccag                                           <  1:3355804/148‑1 (MQ=255)

                                                                                                             tCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGttt                                        >  2:848121/1‑148 (MQ=255)

                                                                                                             tCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGttt                                        >  2:2635755/1‑148 (MQ=255)

                                                                                                             tCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGttt                                        >  1:348579/1‑148 (MQ=255)

                                                                                                                ccccAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGtttttt                                     <  1:2925937/148‑1 (MQ=255)

                                                                                                                ccccAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGtttttt                                     <  1:2925969/148‑1 (MQ=255)

                                                                                                                ccccAGTGACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGtttttt                                     >  1:84279/1‑148 (MQ=255)

                                                                                                                    aGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATTGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACa                                 <  2:2867814/148‑1 (MQ=255)

                                                                                                                    aGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACa                                 >  2:62725/1‑148 (MQ=255)

                                                                                                                    aGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACa                                 <  1:2004561/148‑1 (MQ=255)

                                                                                                                      tGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAg                               <  2:1403909/148‑1 (MQ=255)

                                                                                                                       gACGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGc                              <  2:2499603/148‑1 (MQ=255)

                                                                                                                        aCGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCt                             >  2:2017650/1‑148 (MQ=255)

                                                                                                                        aCGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCt                             <  1:402039/148‑1 (MQ=255)

                                                                                                                        aCGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCt                             >  1:600353/1‑148 (MQ=255)

                                                                                                                        aCGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCt                             >  2:2017571/1‑148 (MQ=255)

                                                                                                                        aCGAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCt                             <  2:363958/148‑1 (MQ=255)

                                                                                                                        aCGAACCGACAATACCCAGCGAATCACAGCCATGGTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCt                             <  2:2441362/148‑1 (MQ=255)

                                                                                                                         cGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTc                            >  2:710131/1‑148 (MQ=255)

                                                                                                                          gAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCg                           >  1:1492816/1‑148 (MQ=255)

                                                                                                                          gAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCg                           <  1:1854691/148‑1 (MQ=255)

                                                                                                                          gAACCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCg                           >  2:1613344/1‑148 (MQ=255)

                                                                                                                            aCCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCt                         <  1:551589/148‑1 (MQ=255)

                                                                                                                            aCCGACAATACCCAGCGAATCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCt                         >  1:1492707/1‑148 (MQ=255)

                                                                                                                             ccGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCtt                        >  2:577927/1‑148 (MQ=255)

                                                                                                                                aCAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTg                     >  2:2198186/1‑148 (MQ=255)

                                                                                                                                     aCCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCagag                <  1:2713011/148‑1 (MQ=255)

                                                                                                                                      cccAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAgc               <  2:292428/148‑1 (MQ=255)

                                                                                                                                               tCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGc      >  1:1735966/1‑148 (MQ=255)

                                                                                                                                               tCACAGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGc      >  2:3239050/1‑148 (MQ=255)

                                                                                                                                                 aCCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGGTCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTg    <  1:1530344/148‑1 (MQ=255)

                                                                                                                                                 aCCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTg    <  2:348579/148‑1 (MQ=255)

                                                                                                                                                   cGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGtt  <  2:3253257/148‑1 (MQ=255)

GGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACAATACCCAGCGAATCACCGCCATGTTCCAGGACAATGGATTCCAGCAGCTCTGCCGCGTCGGAAGGATACGGCGGCAACTGCGGAATGATCATCTCAACGTCAGGGTGATGTTCCGCCAGCCAGTTTTTTAACAAGCTCGCTTTTGCAGAGCGCGGCGAGCTGTT  >  CP009273/3169041‑3169335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`