Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,189,569 C→A 59.4% D182Y (GAT→TAT)  hldE ← fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,189,5690CA59.4% 12.6 / 243.6 174D182Y (GAT→TAT) hldEfused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base C (35/35);  new base A (53/51);  total (88/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-01

Only 100 of 174 total aligned reads displayed.

ACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCA  >  CP009273/3189424‑3189715
                                                                                                                                                 |                                                                                                                                                  
aCTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCgg                                                                                                                                                  >  1:1944509/1‑148 (MQ=255)
aCTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAgg                                                                                                                                                  >  2:3292704/1‑148 (MQ=255)
aCTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAgg                                                                                                                                                  <  1:1441065/148‑1 (MQ=255)
  tAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTa                                                                                                                                                <  2:2848469/148‑1 (MQ=255)
    aCAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTAcc                                                                                                                                              >  1:586847/1‑148 (MQ=255)
    aCAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTAcc                                                                                                                                              >  1:296723/1‑148 (MQ=255)
     cAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCt                                                                                                                                             >  1:5589/1‑148 (MQ=255)
     cAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCt                                                                                                                                             >  2:806745/1‑148 (MQ=255)
     cAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCt                                                                                                                                             >  2:1816850/1‑148 (MQ=255)
     cAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCt                                                                                                                                             <  1:13778/148‑1 (MQ=255)
       gagCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCttt                                                                                                                                           >  2:1167274/1‑148 (MQ=255)
       gagCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCttt                                                                                                                                           >  1:2705697/1‑148 (MQ=255)
        agCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCtttt                                                                                                                                          >  1:1722681/1‑148 (MQ=255)
            gagaGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGat                                                                                                                                      >  1:2874199/1‑148 (MQ=255)
              gagTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatca                                                                                                                                    >  1:1421095/1‑148 (MQ=255)
              gagTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGGAGCGCTCAAATTAGGTACCTGTTGGatca                                                                                                                                    >  1:2237561/1‑148 (MQ=255)
                gTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatcaat                                                                                                                                  <  1:2414042/148‑1 (MQ=255)
                gTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatcaat                                                                                                                                  <  1:2414223/148‑1 (MQ=255)
                 ttCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatcaatc                                                                                                                                 >  2:644150/1‑148 (MQ=255)
                 ttCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatcaatc                                                                                                                                 <  1:3336362/148‑1 (MQ=255)
                  tCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatcaatca                                                                                                                                >  1:3269523/1‑148 (MQ=255)
                    gTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGc                                                                                                                              <  2:3427084/148‑1 (MQ=255)
                    gTAATCGACAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGc                                                                                                                              <  1:1894453/148‑1 (MQ=255)
                         cGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCgg                                                                                                                         >  2:2771891/1‑148 (MQ=255)
                          ggCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTATCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGa                                                                                                                        <  2:2555292/148‑1 (MQ=255)
                          ggCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGa                                                                                                                        <  1:911000/148‑1 (MQ=255)
                           gCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTCGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGac                                                                                                                       >  1:125172/1‑147 (MQ=255)
                            cAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAac                                                                                                                      >  2:1312359/1‑148 (MQ=255)
                             aaTCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAaca                                                                                                                     >  1:1819273/1‑148 (MQ=255)
                                     tCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCttt                                                                                                             >  1:1918638/1‑148 (MQ=255)
                                     tCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCttt                                                                                                             <  2:414947/148‑1 (MQ=255)
                                               cTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGt                                                                                                   >  2:2126672/1‑148 (MQ=255)
                                                tCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGtt                                                                                                  <  1:2508168/148‑1 (MQ=255)
                                                tCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGtt                                                                                                  <  1:2060399/148‑1 (MQ=255)
                                                tCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGtt                                                                                                  <  1:2060152/148‑1 (MQ=255)
                                                       tctcTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcat                                                                                           >  2:218748/1‑148 (MQ=255)
                                                       tctcTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcat                                                                                           <  1:1353194/148‑1 (MQ=255)
                                                        ctctTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcatc                                                                                          >  2:1095450/1‑148 (MQ=255)
                                                        ctctTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcatc                                                                                          <  2:2705697/148‑1 (MQ=255)
                                                         tcttctTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATct                                                                                         >  2:1700545/1‑148 (MQ=255)
                                                         tcttctTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATct                                                                                         >  2:1700709/1‑148 (MQ=255)
                                                          cttcttCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATctg                                                                                        <  2:576828/148‑1 (MQ=255)
                                                          cttcttCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATctg                                                                                        >  2:333783/1‑148 (MQ=255)
                                                            tcttcGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATctgct                                                                                      >  2:262918/1‑148 (MQ=255)
                                                              ttcGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGt                                                                                    <  1:1095450/148‑1 (MQ=255)
                                                                cGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTCGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTAc                                                                                  >  1:2983744/1‑148 (MQ=255)
                                                                cGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTAc                                                                                  >  1:878993/1‑148 (MQ=255)
                                                                  gTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGc                                                                                >  2:1946818/1‑148 (MQ=255)
                                                                  gTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGc                                                                                <  2:1557459/148‑1 (MQ=255)
                                                                   tCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCt                                                                               <  2:124660/148‑1 (MQ=255)
                                                                    cTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCtt                                                                              >  2:692689/1‑148 (MQ=255)
                                                                        cATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCa                                                                          >  2:2717102/1‑148 (MQ=255)
                                                                        cATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCa                                                                          >  2:1667179/1‑148 (MQ=255)
                                                                         aTTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAg                                                                         >  2:2951019/1‑148 (MQ=255)
                                                                               cGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCAcc                                                                   >  2:516455/1‑148 (MQ=255)
                                                                                gACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCt                                                                  >  2:3173278/1‑148 (MQ=255)
                                                                                 acaataGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACACGCTTTACGCGCCAGTTGGATCATCTGATGTACGCTTGCAAGCGCACCtt                                                                 >  2:2017924/1‑148 (MQ=255)
                                                                                   aacaGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTg                                                               >  2:2336022/1‑148 (MQ=255)
                                                                                       gCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGt                                                           >  2:1897420/1‑148 (MQ=255)
                                                                                       gCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGt                                                           >  2:232645/1‑148 (MQ=255)
                                                                                        cTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTa                                                          <  2:2407063/148‑1 (MQ=255)
                                                                                           cAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTc                                                       <  1:1679264/148‑1 (MQ=255)
                                                                                              aTTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGa                                                    <  2:2671298/148‑1 (MQ=255)
                                                                                               ttCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGaa                                                   <  1:455697/148‑1 (MQ=255)
                                                                                               ttCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGaa                                                   >  2:2884541/1‑148 (MQ=255)
                                                                                                 ccGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAg                                                 <  2:2497451/148‑1 (MQ=255)
                                                                                                  cGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGc                                                >  1:369382/1‑148 (MQ=255)
                                                                                                  cGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGc                                                <  1:80981/148‑1 (MQ=255)
                                                                                                    agagaTTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCAc                                              <  1:3549830/148‑1 (MQ=255)
                                                                                                       gaTTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTTCGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAg                                           >  2:234238/1‑148 (MQ=255)
                                                                                                         ttCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAgcg                                         >  1:1921378/1‑148 (MQ=255)
                                                                                                          tCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAgcgc                                        >  1:2510895/1‑148 (MQ=255)
                                                                                                           cGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAgcgcg                                       <  1:3213247/148‑1 (MQ=255)
                                                                                                              cGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCa                                    <  2:616147/148‑1 (MQ=255)
                                                                                                                    cAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGaa                              <  2:1918638/148‑1 (MQ=255)
                                                                                                                    cAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGaa                              >  2:1141950/1‑148 (MQ=255)
                                                                                                                     aGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAAc                             >  1:3469599/1‑148 (MQ=255)
                                                                                                                      gCGTAGCGCCGCGGTAGCGCTCAATATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACt                            <  2:745749/148‑1 (MQ=255)
                                                                                                                      gCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACt                            >  2:2886771/1‑148 (MQ=255)
                                                                                                                       cGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTc                           >  2:1624535/1‑148 (MQ=255)
                                                                                                                        gTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCa                          <  1:1312358/148‑1 (MQ=255)
                                                                                                                           gcgcCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAgcg                       >  1:318941/1‑148 (MQ=255)
                                                                                                                            cgccgcGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAgcgc                      <  1:1667203/148‑1 (MQ=255)
                                                                                                                            cgccgcGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAgcgc                      <  1:790171/148‑1 (MQ=255)
                                                                                                                               cgcgGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTg                   <  1:1946884/148‑1 (MQ=255)
                                                                                                                                gcgGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGa                  <  1:1502258/148‑1 (MQ=255)
                                                                                                                                 cgGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGAt                 <  2:341152/148‑1 (MQ=255)
                                                                                                                                 cgGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATAGAACTCAGCGCCTGAt                 <  1:1166999/148‑1 (MQ=255)
                                                                                                                                   gTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTa               >  2:418856/1‑148 (MQ=255)
                                                                                                                                   gTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTa               <  2:513620/148‑1 (MQ=255)
                                                                                                                                     aGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAAt             <  1:2141310/148‑1 (MQ=255)
                                                                                                                                     aGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAAt             <  2:1932612/148‑1 (MQ=255)
                                                                                                                                        gcTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCg          <  2:3327522/148‑1 (MQ=255)
                                                                                                                                        gcTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCg          <  2:3327217/148‑1 (MQ=255)
                                                                                                                                          tCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCt        <  1:3063938/148‑1 (MQ=255)
                                                                                                                                          tCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCt        <  2:2094568/148‑1 (MQ=255)
                                                                                                                                          tCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCt        <  1:1288046/148‑1 (MQ=255)
                                                                                                                                             aaaTAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGt     >  2:1459455/1‑148 (MQ=255)
                                                                                                                                               aTCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGc   <  1:218748/148‑1 (MQ=255)
                                                                                                                                               aTCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGc   <  1:636904/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCA  >  CP009273/3189424‑3189715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑