BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,205,512	A→C	8.5%	L349L (CTA→CTC)	dnaG →	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,205,512	0	A	C	8.5%	513.1 / 40.7	197	L349L (CTA→CTC)	dnaG	DNA primase
Reads supporting (aligned to +/- strand): ref base A (99/81); new base C (11/6); total (110/87)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 198 total aligned reads displayed.

ACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCATTG  >  CP009273/3205366‑3205659

aCCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAg                                                                                                                                                    <  2:670371/148‑1 (MQ=255)

  caacaaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTa                                                                                                                                                  <  2:2059702/148‑1 (MQ=255)

  caacaaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTa                                                                                                                                                  <  2:1550765/148‑1 (MQ=255)

    acaaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACg                                                                                                                                                >  2:3460726/1‑148 (MQ=255)

     caaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGa                                                                                                                                               >  1:1319183/1‑148 (MQ=255)

     caaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGa                                                                                                                                               >  2:1515796/1‑148 (MQ=255)

       aTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGaaa                                                                                                                                             >  1:674519/1‑148 (MQ=255)

       aTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGaaa                                                                                                                                             >  1:3191319/1‑148 (MQ=255)

        tGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGaaaa                                                                                                                                            >  1:3071018/1‑148 (MQ=255)

              ttGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGt                                                                                                                                      <  1:2556776/148‑1 (MQ=255)

                 cTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTaaa                                                                                                                                   >  1:1155556/1‑148 (MQ=255)

                    ttATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAaagaa                                                                                                                                >  2:921899/1‑148 (MQ=255)

                     tATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAaagaag                                                                                                                               >  1:2230195/1‑148 (MQ=255)

                            gCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGa                                                                                                                        <  1:2851958/148‑1 (MQ=255)

                            gCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGa                                                                                                                        >  1:210771/1‑148 (MQ=255)

                             cGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGaa                                                                                                                       <  1:2285868/148‑1 (MQ=255)

                                ccGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAgcg                                                                                                                    <  1:1575666/148‑1 (MQ=255)

                                ccGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAgcg                                                                                                                    >  2:901442/1‑148 (MQ=255)

                                    gCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGa                                                                                                                >  1:1180673/1‑148 (MQ=255)

                                      aGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATg                                                                                                              <  2:2149356/148‑1 (MQ=255)

                                       ggCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATgg                                                                                                             >  1:1390213/1‑148 (MQ=255)

                                         ccGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAg                                                                                                           <  1:1537198/148‑1 (MQ=255)

                                          cgcgATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGc                                                                                                          <  1:3539775/148‑1 (MQ=255)

                                           gcgATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTGTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCa                                                                                                         <  2:971885/148‑1 (MQ=255)

                                            cgATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAg                                                                                                        >  1:634984/1‑148 (MQ=255)

                                            cgATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAg                                                                                                        >  2:1577846/1‑148 (MQ=255)

                                              aTGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGACAAGACGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGc                                                                                                      >  2:365330/1‑148 (MQ=255)

                                               tGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCg                                                                                                     >  1:412662/1‑148 (MQ=255)

                                               tGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCg                                                                                                     >  1:2207173/1‑148 (MQ=255)

                                                gccgccTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGa                                                                                                    >  1:1676012/1‑148 (MQ=255)

                                                    ccTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGGACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCTGATGTAGCAGGCGATGcc                                                                                                >  2:3433776/1‑148 (MQ=255)

                                                        gCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCActc                                                                                            >  1:2696710/1‑148 (MQ=255)

                                                         cGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCActct                                                                                           >  2:575778/1‑148 (MQ=255)

                                                            gcgcTGGAAACGGCGCTGCCTTACATGACAGCCGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCg                                                                                        >  2:3335630/1‑148 (MQ=255)

                                                             cgcTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGc                                                                                       <  2:2230195/148‑1 (MQ=255)

                                                             cgcTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGc                                                                                       <  2:1008706/148‑1 (MQ=255)

                                                                tGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCAtt                                                                                    <  2:635972/148‑1 (MQ=255)

                                                                tGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCAtt                                                                                    <  2:636897/148‑1 (MQ=255)

                                                                 ggAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCAttt                                                                                   <  2:2420583/148‑1 (MQ=255)

                                                                   aaaCGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCt                                                                                 >  1:3101359/1‑148 (MQ=255)

                                                                     aCGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGt                                                                               <  2:1205694/148‑1 (MQ=255)

                                                                     aCGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGt                                                                               <  2:2979610/148‑1 (MQ=255)

                                                                      cGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGtt                                                                              <  1:3015215/148‑1 (MQ=255)

                                                                          gcTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAAc                                                                          <  1:950282/148‑1 (MQ=255)

                                                                          gcTGCCTTACATGACAGACGGCCGTCAGATACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAAc                                                                          <  1:1297516/148‑1 (MQ=255)

                                                                           cTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACa                                                                         <  1:1601183/148‑1 (MQ=255)

                                                                           cTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACa                                                                         >  1:1795253/1‑148 (MQ=255)

                                                                            tGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAg                                                                        >  1:2021427/1‑148 (MQ=255)

                                                                             gCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACCAGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGt                                                                       <  1:2686280/148‑1 (MQ=255)

                                                                             gCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGt                                                                       <  1:1031261/148‑1 (MQ=255)

                                                                             gCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAACACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGt                                                                       >  2:2910281/1‑148 (MQ=255)

                                                                              ccTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTc                                                                      <  2:489999/148‑1 (MQ=255)

                                                                                ttACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTg                                                                    <  2:743772/148‑1 (MQ=255)

                                                                                ttACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTg                                                                    >  1:3142244/1‑148 (MQ=255)

                                                                                ttACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTg                                                                    >  1:2214766/1‑148 (MQ=255)

                                                                                     tGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGcc                                                               >  2:1488905/1‑148 (MQ=255)

                                                                                      gACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCg                                                              >  1:3479484/1‑148 (MQ=255)

                                                                                           aCGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGt                                                         <  1:566787/148‑1 (MQ=255)

                                                                                           aCGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGt                                                         >  2:2384103/1‑148 (MQ=255)

                                                                                              gCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGa                                                      >  2:3182372/1‑148 (MQ=255)

                                                                                              gCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGa                                                      <  1:2515987/148‑1 (MQ=255)

                                                                                              gCCGGCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGa                                                      >  2:1662207/1‑148 (MQ=255)

                                                                                               ccGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGAt                                                     <  2:3070551/148‑1 (MQ=255)

                                                                                               ccGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGAt                                                     >  2:1971017/1‑148 (MQ=255)

                                                                                                  tCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTg                                                  <  2:2104199/148‑1 (MQ=255)

                                                                                                        aCGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTAcc                                            <  1:3026505/148‑1 (MQ=255)

                                                                                                        aCGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTAcc                                            <  1:3026464/148‑1 (MQ=255)

                                                                                                        aCGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTAcc                                            <  2:161935/148‑1 (MQ=255)

                                                                                                         cGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTAccc                                           <  1:1515811/148‑1 (MQ=255)

                                                                                                         cGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTAccc                                           >  1:2877578/1‑148 (MQ=255)

                                                                                                             ttATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGa                                       >  1:1700100/1‑148 (MQ=255)

                                                                                                             ttATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGa                                       >  2:1183602/1‑148 (MQ=255)

                                                                                                             ttATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGa                                       >  2:1295062/1‑148 (MQ=255)

                                                                                                               aTGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACg                                     <  2:2696710/148‑1 (MQ=255)

                                                                                                                tGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACgg                                    >  2:1646462/1‑148 (MQ=255)

                                                                                                                tGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACgg                                    <  1:3170862/148‑1 (MQ=255)

                                                                                                                 gTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACggg                                   <  1:3245918/148‑1 (MQ=255)

                                                                                                                  tttttGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGgc                                  >  1:813989/1‑148 (MQ=255)

                                                                                                                      tGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGgcgcgc                              >  2:1137617/1‑148 (MQ=255)

                                                                                                                      tGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGgcgcgc                              >  2:2619436/1‑148 (MQ=255)

                                                                                                                         cTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACg                           <  1:1038994/148‑1 (MQ=255)

                                                                                                                          tGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGt                          >  2:2824621/1‑148 (MQ=255)

                                                                                                                           gATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGtt                         >  1:1388001/1‑148 (MQ=255)

                                                                                                                            aTGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGttt                        >  2:1878363/1‑148 (MQ=255)

                                                                                                                             tGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTg                       <  2:1319183/148‑1 (MQ=255)

                                                                                                                             tGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTg                       <  2:1051012/148‑1 (MQ=255)

                                                                                                                             tGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTg                       <  2:1051441/148‑1 (MQ=255)

                                                                                                                                cGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGt                    <  1:1453090/148‑1 (MQ=255)

                                                                                                                                  aaGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTAc                  >  1:3558826/1‑148 (MQ=255)

                                                                                                                                   aGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACg                 >  2:2489075/1‑148 (MQ=255)

                                                                                                                                   aGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACg                 <  1:906456/148‑1 (MQ=255)

                                                                                                                                    gACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGc                >  2:2550823/1‑148 (MQ=255)

                                                                                                                                    gACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGc                >  2:1988545/1‑148 (MQ=255)

                                                                                                                                     aCCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCt               >  2:3102087/1‑148 (MQ=255)

                                                                                                                                         tGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCa           >  1:1365175/1‑148 (MQ=255)

                                                                                                                                           acacGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACt         >  2:1551815/1‑148 (MQ=255)

                                                                                                                                           acacGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACt         >  1:1546557/1‑148 (MQ=255)

                                                                                                                                                cTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCAt    >  2:693486/1‑148 (MQ=255)

                                                                                                                                                cTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCAt    >  1:2969607/1‑148 (MQ=255)

                                                                                                                                                  aGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCATTg  <  1:3460726/148‑1 (MQ=255)

ACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCATTG  >  CP009273/3205366‑3205659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`