Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,212,264 G→T 24.5% H58N (CAC→AAC)  aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,212,2640GT24.5% 505.6 / 120.3 216H58N (CAC→AAC) aerfused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base G (87/76);  new base T (29/24);  total (116/100)
Fisher's exact test for biased strand distribution p-value = 8.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 217 total aligned reads displayed.

TTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCT  >  CP009273/3212117‑3212410
                                                                                                                                                   |                                                                                                                                                  
    cTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGc                                                                                                                                                >  1:208747/1‑148 (MQ=255)
    cTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGc                                                                                                                                                <  2:1836701/148‑1 (MQ=255)
      cgcgCACCATCGGTACCTCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGGTATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACAACTTATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCAc                                                                                                                                              <  1:1348266/148‑1 (MQ=255)
          cACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCAtg                                                                                                                                          <  1:43020/148‑1 (MQ=255)
           aCCATCGGTCCCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGGTACGCACCAggt                                                                                                                                         >  2:1536129/1‑148 (MQ=255)
           aCCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCAtgt                                                                                                                                         <  1:2044931/148‑1 (MQ=255)
             cATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCAtgttg                                                                                                                                       <  2:2767794/148‑1 (MQ=255)
             cATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCAtgttg                                                                                                                                       >  2:543226/1‑148 (MQ=255)
             cATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCAtgttg                                                                                                                                       >  2:543208/1‑148 (MQ=255)
               tCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTtgtg                                                                                                                                     <  1:2296095/148‑1 (MQ=255)
               tCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTtgtg                                                                                                                                     >  1:1641371/1‑148 (MQ=255)
               tCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTtgtg                                                                                                                                     <  1:3370524/148‑1 (MQ=255)
                  ttACAGCATTTGCCCGGACCCAATATTTGTCACCATTTTTTAGGGGATTTTTCACTATGCAGCTCCATGGCTCCCCTTTTTTCTTGGTGAACCACATATCCGCATACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCgg                                                                                                                                  <  2:1345906/147‑1 (MQ=255)
                  gTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCgg                                                                                                                                  <  1:874082/148‑1 (MQ=255)
                       gCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGcc                                                                                                                             <  2:2486293/148‑1 (MQ=255)
                        cATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCACACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGccc                                                                                                                            <  1:2406232/148‑1 (MQ=255)
                         aTTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCt                                                                                                                           <  2:921891/148‑1 (MQ=255)
                           tGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTg                                                                                                                         >  1:2491991/1‑148 (MQ=255)
                             gCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTa                                                                                                                       >  1:830439/1‑148 (MQ=255)
                                       aataatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCaa                                                                                                             >  2:645842/1‑148 (MQ=255)
                                          aatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGt                                                                                                          >  2:91931/1‑148 (MQ=255)
                                          aatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCACAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGt                                                                                                          >  1:1335261/1‑148 (MQ=255)
                                             ggTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACTATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGtata                                                                                                       >  1:2962120/1‑148 (MQ=255)
                                             ggTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACTATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGtata                                                                                                       >  1:2860366/1‑148 (MQ=255)
                                             ggTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTGGTAACTCTTGCAAGGtata                                                                                                       >  2:3407735/1‑148 (MQ=255)
                                                 aCCATTTTTGCGGCGATTTTTCACGATGCCGCTCCATGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCg                                                                                                   >  1:3007783/1‑148 (MQ=255)
                                                 aCCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCg                                                                                                   <  1:3085718/148‑1 (MQ=255)
                                                 aCCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCg                                                                                                   <  1:259779/148‑1 (MQ=255)
                                                   cATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCt                                                                                                 >  1:1027947/1‑148 (MQ=255)
                                                    aTTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCCGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCACTc                                                                                                <  2:2860089/148‑1 (MQ=255)
                                                      ttttGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCaa                                                                                              >  2:777529/1‑147 (MQ=255)
                                                      ttttGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCAc                                                                                              <  2:489771/148‑1 (MQ=255)
                                                           cggcgATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGca                                                                                         <  2:1953981/148‑1 (MQ=255)
                                                              cgATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACaa                                                                                      >  2:2707817/1‑148 (MQ=255)
                                                                 tttttCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCCCCTGCACAAAAg                                                                                   >  1:336380/1‑148 (MQ=255)
                                                                 tttttCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACACAAg                                                                                   <  2:2439196/148‑1 (MQ=255)
                                                                  ttttCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAgt                                                                                  <  2:1511147/148‑1 (MQ=255)
                                                                  ttttCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAgt                                                                                  >  1:3268057/1‑148 (MQ=255)
                                                                   tttCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAgtg                                                                                 <  1:1197706/148‑1 (MQ=255)
                                                                     tCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTc                                                                               >  1:2884497/1‑148 (MQ=255)
                                                                       aCGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCAt                                                                             >  2:2213461/1‑148 (MQ=255)
                                                                          aTGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAg                                                                          >  1:404211/1‑148 (MQ=255)
                                                                            gCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTGTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCa                                                                        >  1:666875/1‑148 (MQ=255)
                                                                             ccGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCAt                                                                       <  1:2258803/148‑1 (MQ=255)
                                                                              cGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATg                                                                      <  1:1417289/148‑1 (MQ=255)
                                                                               gCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGa                                                                     <  2:167427/148‑1 (MQ=255)
                                                                                cTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAg                                                                    <  2:268150/148‑1 (MQ=255)
                                                                                cTCCAGGGCTCCCCTATTTTCAGCGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCAGCCCTTGTAACTCTTTCAAGGTATAGACGCTCACCTGCACAAAAGAGTCATTAGCAagag                                                                    >  2:2334610/1‑148 (MQ=255)
                                                                                 tCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGt                                                                   <  2:2417952/148‑1 (MQ=255)
                                                                                   cAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGa                                                                 <  2:208747/148‑1 (MQ=255)
                                                                                    aGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGat                                                                >  2:2995692/1‑148 (MQ=255)
                                                                                    aGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGat                                                                >  2:815584/1‑148 (MQ=255)
                                                                                      ggCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGatat                                                              <  1:145525/148‑1 (MQ=255)
                                                                                      ggCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGatat                                                              <  2:2712383/148‑1 (MQ=255)
                                                                                      ggCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGatat                                                              <  1:1823431/148‑1 (MQ=255)
                                                                                      ggCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGatat                                                              <  1:2740297/148‑1 (MQ=255)
                                                                                      ggCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTGTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGatat                                                              >  2:2176623/1‑148 (MQ=255)
                                                                                        cTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAg                                                            >  1:2536005/1‑148 (MQ=255)
                                                                                         tCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGc                                                           <  2:1734508/148‑1 (MQ=255)
                                                                                         tCCCCTTTTTTCAGGGTGAACCACATATCAGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGc                                                           <  1:1802779/148‑1 (MQ=255)
                                                                                          ccccTTTTTTCAGGGTGAACCACATCTCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCt                                                          <  1:1983270/148‑1 (MQ=255)
                                                                                          ccccTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCt                                                          <  1:1951389/148‑1 (MQ=255)
                                                                                           cccTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCtt                                                         <  1:499295/148‑1 (MQ=255)
                                                                                            ccTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCttt                                                        >  2:1300905/1‑148 (MQ=255)
                                                                                                     aGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCgg                                               <  2:1395959/148‑1 (MQ=255)
                                                                                                      gggTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGt                                              >  2:1067763/1‑148 (MQ=255)
                                                                                                       ggTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTa                                             >  1:1874244/1‑148 (MQ=255)
                                                                                                           aaCCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTgg                                         >  1:3410750/1‑148 (MQ=255)
                                                                                                              cacaTATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACa                                      <  2:1505523/148‑1 (MQ=255)
                                                                                                               acaTATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACAt                                     >  2:3309409/1‑148 (MQ=255)
                                                                                                               acaTATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACAt                                     >  1:304802/1‑148 (MQ=255)
                                                                                                               acaTATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACAt                                     >  1:1267942/1‑148 (MQ=255)
                                                                                                                caTATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATc                                    >  1:711447/1‑148 (MQ=255)
                                                                                                                caTATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATc                                    >  1:623622/1‑148 (MQ=255)
                                                                                                                 atatCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCa                                   >  2:2375337/1‑148 (MQ=255)
                                                                                                                 atatCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCa                                   >  2:1396966/1‑148 (MQ=255)
                                                                                                                  tatCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCag                                  >  1:1713188/1‑148 (MQ=255)
                                                                                                                   atCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCaga                                 >  1:3502576/1‑148 (MQ=255)
                                                                                                                   atCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCaga                                 >  1:3502592/1‑148 (MQ=255)
                                                                                                                   atCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCaga                                 <  1:156633/148‑1 (MQ=255)
                                                                                                                   atCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCaga                                 >  1:1527280/1‑148 (MQ=255)
                                                                                                                     ccGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGt                               <  2:3202708/148‑1 (MQ=255)
                                                                                                                     ccGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGt                               <  2:519100/148‑1 (MQ=255)
                                                                                                                     ccGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGt                               <  2:3142173/148‑1 (MQ=255)
                                                                                                                      cGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTg                              <  1:2213461/148‑1 (MQ=255)
                                                                                                                       gCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTgg                             >  1:794671/1‑148 (MQ=255)
                                                                                                                       gCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTgg                             <  2:1096121/148‑1 (MQ=255)
                                                                                                                         aaaCGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTa                           <  2:2884506/148‑1 (MQ=255)
                                                                                                                         aaaCGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTa                           <  1:651633/148‑1 (MQ=255)
                                                                                                                          aaCGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTAt                          >  2:1615151/1‑148 (MQ=255)
                                                                                                                           aCGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATc                         >  2:2536399/1‑148 (MQ=255)
                                                                                                                              ccgcTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTc                      >  2:1877130/1‑148 (MQ=255)
                                                                                                                                 cTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGc                   >  2:301131/1‑148 (MQ=255)
                                                                                                                                    ttGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAg                <  2:3279294/148‑1 (MQ=255)
                                                                                                                                           atCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTa         >  2:3498095/1‑148 (MQ=255)
                                                                                                                                            tCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAt        <  1:1173802/148‑1 (MQ=255)
                                                                                                                                             ccGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAtt       >  1:828353/1‑148 (MQ=255)
                                                                                                                                             ccGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAtt       >  1:247573/1‑148 (MQ=255)
                                                                                                                                                ggTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTg    <  1:2319458/148‑1 (MQ=255)
                                                                                                                                                  tGACGCACCATGTTGTGCGGCTGCCCTGGTAACTCTTGCACGGGATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGAGATCGGTGTGGCGGTCGGGCGTGGCCAGCGGGGTGGTCTGGTCCGCCCGGGGGGGAGTTTggg  >  1:2440341/1‑146 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCT  >  CP009273/3212117‑3212410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑