Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,266,646 C→A 100% G91C (GGT→TGT)  garL ← alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,266,6460CA98.6% 671.0 / ‑0.7 209G91C (GGT→TGT) garLalpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (113/93);  total (115/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

AAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCC  >  CP009273/3266501‑3266789
                                                                                                                                                 |                                                                                                                                               
 aataatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGat                                                                                                                                              >  2:3138343/1‑148 (MQ=255)
   taatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGatat                                                                                                                                            >  1:2054105/1‑148 (MQ=255)
   taatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGatat                                                                                                                                            <  1:713016/148‑1 (MQ=255)
   taatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGatat                                                                                                                                            <  1:2732486/148‑1 (MQ=255)
    aatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATc                                                                                                                                           >  1:1082324/1‑148 (MQ=255)
     atCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATcc                                                                                                                                          >  1:1643079/1‑148 (MQ=255)
      tCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCACCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCa                                                                                                                                         <  1:1290137/148‑1 (MQ=255)
        cGCCACGGTTCCAAACATATGGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCaga                                                                                                                                       <  2:3375196/148‑1 (MQ=255)
        cGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGACGTTATAGAAACAGATATCCaga                                                                                                                                       >  2:2089188/1‑148 (MQ=255)
        cGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCaga                                                                                                                                       <  1:3502131/148‑1 (MQ=255)
              ggTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGc                                                                                                                                 <  2:1935549/148‑1 (MQ=255)
              ggTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGc                                                                                                                                 <  2:642643/148‑1 (MQ=255)
              ggTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGc                                                                                                                                 <  2:1547087/148‑1 (MQ=255)
               gTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCt                                                                                                                                <  1:2843972/148‑1 (MQ=255)
                   cAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTtaat                                                                                                                            <  1:77622/148‑1 (MQ=255)
                     aaCATAGTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTtaataa                                                                                                                          <  2:433379/148‑1 (MQ=255)
                      aCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTaataat                                                                                                                         <  1:412254/148‑1 (MQ=255)
                      aCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTaataat                                                                                                                         <  1:2476027/148‑1 (MQ=255)
                       cATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAAtt                                                                                                                        >  2:1653515/1‑148 (MQ=255)
                        atatTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTa                                                                                                                       <  2:2760762/148‑1 (MQ=255)
                         tatTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTAc                                                                                                                      <  2:331538/148‑1 (MQ=255)
                         tatTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTAc                                                                                                                      <  2:2067129/148‑1 (MQ=255)
                          atTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCCTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTAcc                                                                                                                     <  1:3179025/148‑1 (MQ=255)
                              gcgcGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCt                                                                                                                 >  1:3225410/1‑148 (MQ=255)
                                gcgGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCg                                                                                                               <  1:1816937/148‑1 (MQ=255)
                                 cgGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGt                                                                                                              <  1:548826/148‑1 (MQ=255)
                                     gagaAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGt                                                                                                          <  1:236267/148‑1 (MQ=255)
                                     gagaAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGt                                                                                                          <  1:2031783/148‑1 (MQ=255)
                                        aaaCGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCgg                                                                                                       <  2:2855423/148‑1 (MQ=255)
                                        aaaCGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCgg                                                                                                       >  1:2357896/1‑148 (MQ=255)
                                        aaaCGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCgg                                                                                                       >  1:1583595/1‑148 (MQ=255)
                                        aaaCGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCgg                                                                                                       >  2:3155831/1‑148 (MQ=255)
                                           cGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGacac                                                                                                    >  2:1571310/1‑148 (MQ=255)
                                           cGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGacac                                                                                                    >  2:1571330/1‑148 (MQ=255)
                                           cGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCAc                                                                                                    >  2:849878/1‑148 (MQ=255)
                                             gagaCGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTc                                                                                                  <  2:3432270/148‑1 (MQ=255)
                                                aCGCCGCGAATGCCTTCCGGTGGGTCACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCa                                                                                               >  2:1366086/1‑148 (MQ=255)
                                                         aTGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgcg                                                                                      >  2:3389523/1‑148 (MQ=255)
                                                         aTGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgcg                                                                                      >  2:3305365/1‑148 (MQ=255)
                                                           gCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgcgcg                                                                                    >  1:3088369/1‑148 (MQ=255)
                                                           gCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgcgcg                                                                                    <  1:1982253/148‑1 (MQ=255)
                                                              ttCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctg                                                                                 >  1:135785/1‑148 (MQ=255)
                                                              ttCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctg                                                                                 >  1:2818285/1‑148 (MQ=255)
                                                               tCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctgg                                                                                >  2:1149716/1‑148 (MQ=255)
                                                                      ggTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGcc                                                                         <  2:3076254/148‑1 (MQ=255)
                                                                       gTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCt                                                                        <  2:3125619/148‑1 (MQ=255)
                                                                       gTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCt                                                                        <  2:3245124/148‑1 (MQ=255)
                                                                         aaCGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCttt                                                                      <  2:1869981/148‑1 (MQ=255)
                                                                         aaCGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCttt                                                                      <  2:200816/148‑1 (MQ=255)
                                                                         aaCGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCttt                                                                      >  2:2169526/1‑148 (MQ=255)
                                                                           cGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCa                                                                    <  2:2576422/148‑1 (MQ=255)
                                                                           cGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCa                                                                    >  2:2288953/1‑148 (MQ=255)
                                                                             ggTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAg                                                                  <  1:1680092/148‑1 (MQ=255)
                                                                             ggTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGACACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAg                                                                  >  1:651274/1‑148 (MQ=255)
                                                                              gTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAgg                                                                 <  1:1737560/148‑1 (MQ=255)
                                                                                    gCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTa                                                           <  2:587623/148‑1 (MQ=255)
                                                                                     ccACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTaa                                                          >  1:21729/1‑148 (MQ=255)
                                                                                            cAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGa                                                   >  1:994000/1‑148 (MQ=255)
                                                                                            cAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGa                                                   <  1:2696119/148‑1 (MQ=255)
                                                                                             aGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGaa                                                  <  1:3157739/148‑1 (MQ=255)
                                                                                             aGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGaa                                                  >  1:1166064/1‑148 (MQ=255)
                                                                                             aGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGaa                                                  >  1:3356110/1‑148 (MQ=255)
                                                                                              gCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAAt                                                 >  2:317256/1‑148 (MQ=255)
                                                                                              gCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAAt                                                 >  2:49734/1‑148 (MQ=255)
                                                                                              gCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAAt                                                 >  1:1469294/1‑148 (MQ=255)
                                                                                                 ctGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATaaa                                              >  2:1734542/1‑148 (MQ=255)
                                                                                                 ctGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATaaa                                              >  2:3495283/1‑148 (MQ=255)
                                                                                                 ctGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATaaa                                              >  1:2703858/1‑148 (MQ=255)
                                                                                                  tGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAAc                                             >  1:2560688/1‑148 (MQ=255)
                                                                                                   gCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACg                                            >  1:1828422/1‑148 (MQ=255)
                                                                                                    cTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGt                                           <  2:3238349/148‑1 (MQ=255)
                                                                                                    cTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGt                                           <  2:1567962/148‑1 (MQ=255)
                                                                                                      tCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTgg                                         >  2:2383479/1‑148 (MQ=255)
                                                                                                        ctctTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGgag                                       >  2:225056/1‑148 (MQ=255)
                                                                                                        ctctTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGgag                                       <  1:1918627/148‑1 (MQ=255)
                                                                                                          ctTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGat                                     >  2:2441176/1‑148 (MQ=255)
                                                                                                           ttttGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGata                                    <  2:1033960/148‑1 (MQ=255)
                                                                                                              tGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCg                                 >  2:2658788/1‑148 (MQ=255)
                                                                                                                tttCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGtt                               >  2:179540/1‑148 (MQ=255)
                                                                                                                 ttCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGttt                              >  2:231148/1‑148 (MQ=255)
                                                                                                                 ttCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGttt                              >  1:1878559/1‑148 (MQ=255)
                                                                                                                     aCAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGgcg                          >  1:2566180/1‑148 (MQ=255)
                                                                                                                        aaaGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCAt                       >  1:2974465/1‑148 (MQ=255)
                                                                                                                          aGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGt                     >  2:1406807/1‑148 (MQ=255)
                                                                                                                          aGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGt                     >  2:1406483/1‑148 (MQ=255)
                                                                                                                           ggAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGtt                    >  1:1598870/1‑148 (MQ=255)
                                                                                                                           ggAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGtt                    >  2:1048645/1‑148 (MQ=255)
                                                                                                                           ggAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGtt                    >  2:2767231/1‑148 (MQ=255)
                                                                                                                                 aGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCAt              >  1:3113568/1‑148 (MQ=255)
                                                                                                                                    aaGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCa           >  1:1852603/1‑148 (MQ=255)
                                                                                                                                    aaGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCa           >  2:166638/1‑148 (MQ=255)
                                                                                                                                       ttATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCa        >  1:1549439/1‑148 (MQ=255)
                                                                                                                                         ataGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAcc      >  2:2937000/1‑148 (MQ=255)
                                                                                                                                          taGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGACACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAcca     <  1:1571330/148‑1 (MQ=255)
                                                                                                                                          taGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGACACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAcca     <  1:1571311/148‑1 (MQ=255)
                                                                                                                                          taGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGACACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAcca     <  1:1571297/148‑1 (MQ=255)
                                                                                                                                           aGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAccag    >  2:1617171/1‑148 (MQ=255)
                                                                                                                                           aGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAccag    <  2:2159185/148‑1 (MQ=255)
                                                                                                                                             aaaCAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAccagcc  <  2:2546679/148‑1 (MQ=255)
                                                                                                                                             aaaCAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAccagcc  <  2:218086/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCC  >  CP009273/3266501‑3266789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑