Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,312,055 C→A 100% G20G (GGC→GGA)  argG → argininosuccinate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,312,0550CA97.4% 598.8 / 6.1 192G20G (GGC→GGA) argGargininosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (87/100);  total (88/104)
Fisher's exact test for biased strand distribution p-value = 3.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 192 total aligned reads displayed.

ATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCGCGGAGAACG  >  CP009273/3311911‑3312197
                                                                                                                                                |                                                                                                                                              
aTGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGt                                                                                                                                             >  2:698027/1‑148 (MQ=255)
 tGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTc                                                                                                                                            >  2:1651717/1‑148 (MQ=255)
  gAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCt                                                                                                                                           >  1:2608078/1‑148 (MQ=255)
   aaaaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTg                                                                                                                                          >  1:654793/1‑148 (MQ=255)
    aaaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTgg                                                                                                                                         <  1:3517090/148‑1 (MQ=255)
     aaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGa                                                                                                                                        >  1:131140/1‑148 (MQ=255)
         cATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACAcc                                                                                                                                    >  2:2783214/1‑148 (MQ=255)
         cATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACAcc                                                                                                                                    <  2:349076/148‑1 (MQ=255)
           tttatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAg                                                                                                                                  >  2:2957907/1‑148 (MQ=255)
           tttatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAg                                                                                                                                  >  2:1592927/1‑148 (MQ=255)
            ttatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGt                                                                                                                                 >  1:1089768/1‑148 (MQ=255)
             tatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTg                                                                                                                                <  1:1706758/148‑1 (MQ=255)
              atttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGc                                                                                                                               >  1:1852736/1‑148 (MQ=255)
               ttttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGcc                                                                                                                              >  1:2014586/1‑148 (MQ=255)
                tttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCg                                                                                                                             <  2:737596/148‑1 (MQ=255)
                 ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGc                                                                                                                            <  2:2103056/148‑1 (MQ=255)
                    cATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCAct                                                                                                                         >  2:1271983/1‑148 (MQ=255)
                     aTAAAAAGTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCActg                                                                                                                        <  2:3495711/148‑1 (MQ=255)
                       aaaaaTTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCActgct                                                                                                                      <  1:3319778/148‑1 (MQ=255)
                            ttCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGa                                                                                                                 <  2:3499246/148‑1 (MQ=255)
                            ttCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGa                                                                                                                 >  2:3202322/1‑148 (MQ=255)
                             tCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGAt                                                                                                                >  2:1211755/1‑148 (MQ=255)
                             tCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGAt                                                                                                                >  1:1465256/1‑148 (MQ=255)
                              cAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATg                                                                                                               <  1:1183136/148‑1 (MQ=255)
                               aGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGc                                                                                                              >  1:947016/1‑148 (MQ=255)
                                 tGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGa                                                                                                            >  2:2529194/1‑148 (MQ=255)
                                  gagaGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGAc                                                                                                           >  2:94157/1‑148 (MQ=255)
                                     agCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACaaa                                                                                                        >  2:1635460/1‑148 (MQ=255)
                                      gCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACaaaa                                                                                                       <  1:2818211/148‑1 (MQ=255)
                                           aaTCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAg                                                                                                  <  1:3357689/148‑1 (MQ=255)
                                            aTCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGc                                                                                                 >  1:2378168/1‑148 (MQ=255)
                                            aTCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGc                                                                                                 >  2:1403092/1‑148 (MQ=255)
                                            aTCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGc                                                                                                 >  2:1402603/1‑148 (MQ=255)
                                             tCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCg                                                                                                <  2:3022432/148‑1 (MQ=255)
                                             tCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCg                                                                                                >  1:1774316/1‑148 (MQ=255)
                                               cAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGt                                                                                              <  2:2608078/148‑1 (MQ=255)
                                                aGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGtt                                                                                             >  1:2392190/1‑148 (MQ=255)
                                                aGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGtt                                                                                             >  1:986127/1‑148 (MQ=255)
                                                 ggCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTc                                                                                            >  1:1956682/1‑148 (MQ=255)
                                                  gCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTcc                                                                                           <  2:642766/148‑1 (MQ=255)
                                                    tcatcatcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCtt                                                                                         <  2:2390481/148‑1 (MQ=255)
                                                     catcatcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTa                                                                                        <  1:3108818/148‑1 (MQ=255)
                                                     catcatcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTa                                                                                        <  2:2584005/148‑1 (MQ=255)
                                                     catcatcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTa                                                                                        <  1:1510262/148‑1 (MQ=255)
                                                     catcatcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGCTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGAACTGATGTGGATGCGACAAAAGGGAGCGGTTCCTTa                                                                                        >  1:2715361/1‑148 (MQ=255)
                                                       tcatcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATg                                                                                      >  2:13251/1‑148 (MQ=255)
                                                        catcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGc                                                                                     >  1:2784707/1‑148 (MQ=255)
                                                         atcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCa                                                                                    >  1:2118037/1‑148 (MQ=255)
                                                          tcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCat                                                                                   <  2:2147467/148‑1 (MQ=255)
                                                          tcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCat                                                                                   >  2:1937945/1‑148 (MQ=255)
                                                          tcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCat                                                                                   <  1:173475/148‑1 (MQ=255)
                                                           caGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCata                                                                                  <  1:2145629/148‑1 (MQ=255)
                                                            aGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCatat                                                                                 <  2:46272/148‑1 (MQ=255)
                                                            aGTTAATGAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCatat                                                                                 <  2:46245/148‑1 (MQ=255)
                                                             gTTAATTAAGCAGTGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCAtata                                                                                >  1:2605089/1‑148 (MQ=255)
                                                             gTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCAtata                                                                                <  2:2784707/148‑1 (MQ=255)
                                                             gTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCAtata                                                                                <  2:265716/148‑1 (MQ=255)
                                                                  ttaaGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCa                                                                           >  2:2846230/1‑148 (MQ=255)
                                                                   taaGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCaa                                                                          <  1:991383/148‑1 (MQ=255)
                                                                           gtgtTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAAAGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGgc                                                                  <  1:1048920/148‑1 (MQ=255)
                                                                                aTTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGgccagcc                                                             <  1:1232355/148‑1 (MQ=255)
                                                                                 ttttATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGgccagcca                                                            >  2:847656/1‑148 (MQ=255)
                                                                                      tGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGa                                                       <  2:3405812/148‑1 (MQ=255)
                                                                                      tGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGa                                                       >  1:1191905/1‑148 (MQ=255)
                                                                                         cgacgaTGCTCAAGCCACTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAaga                                                    <  2:107028/148‑1 (MQ=255)
                                                                                             gaTTCTCAAGCATCTCCCGGTAGGTCAACGTTTTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGAt                                                <  2:3114473/148‑1 (MQ=255)
                                                                                                tctcAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTat                                             >  2:1971114/1‑148 (MQ=255)
                                                                                                 ctcAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatg                                            <  2:1850153/148‑1 (MQ=255)
                                                                                                 ctcAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatg                                            <  2:2162563/148‑1 (MQ=255)
                                                                                                   cAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatgat                                          <  1:698027/148‑1 (MQ=255)
                                                                                                   cAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatgat                                          >  2:3336101/1‑148 (MQ=255)
                                                                                                   cAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatgat                                          >  1:3532615/1‑148 (MQ=255)
                                                                                                   cAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatgat                                          <  1:94157/148‑1 (MQ=255)
                                                                                                      gCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCg                                       >  1:789172/1‑148 (MQ=255)
                                                                                                      gCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCg                                       <  1:141752/148‑1 (MQ=255)
                                                                                                       cATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGa                                      <  1:474465/148‑1 (MQ=255)
                                                                                                        aTCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGAt                                     <  1:1259759/148‑1 (MQ=255)
                                                                                                         tctcCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATc                                    >  1:553207/1‑148 (MQ=255)
                                                                                                          ctcCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATcc                                   <  1:2882205/148‑1 (MQ=255)
                                                                                                           tcCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATccc                                  <  1:2601177/148‑1 (MQ=255)
                                                                                                           tcCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATccc                                  <  2:1791777/148‑1 (MQ=255)
                                                                                                           tcCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATccc                                  <  1:2313437/148‑1 (MQ=255)
                                                                                                            cccGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCt                                 <  2:1497238/148‑1 (MQ=255)
                                                                                                             ccGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCtc                                <  2:1301655/148‑1 (MQ=255)
                                                                                                                    gTCAACGTATTGGTATCGCTTTTTCTGGCGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGc                         <  1:845878/148‑1 (MQ=255)
                                                                                                                    gTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATTCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGc                         >  1:1217616/1‑148 (MQ=255)
                                                                                                                         cGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATgg                    >  2:1679553/1‑148 (MQ=255)
                                                                                                                          gTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGa                   <  1:2757060/148‑1 (MQ=255)
                                                                                                                           tATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGaa                  >  1:2071132/1‑148 (MQ=255)
                                                                                                                           tATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGaa                  <  1:3202322/148‑1 (MQ=255)
                                                                                                                            aTTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAAt                 <  2:542937/148‑1 (MQ=255)
                                                                                                                              tGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATAc               >  1:2677080/1‑148 (MQ=255)
                                                                                                                               ggTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACg              <  1:141600/148‑1 (MQ=255)
                                                                                                                                gTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACgg             <  2:2258688/148‑1 (MQ=255)
                                                                                                                                gTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACgg             >  1:3469032/1‑148 (MQ=255)
                                                                                                                                   tCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGgcgc          >  1:1803301/1‑148 (MQ=255)
                                                                                                                                    cGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGcgcg         >  1:3056842/1‑148 (MQ=255)
                                                                                                                                     gCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCGCgg        >  2:810594/1‑148 (MQ=255)
                                                                                                                                       tttttCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCGCGgag      <  2:3560930/148‑1 (MQ=255)
                                                                                                                                           tCTGGCGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCGCGGAGAACg  <  2:2080140/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
ATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCGCGGAGAACG  >  CP009273/3311911‑3312197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑