Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,396,082 G→T 100% R204S (CGT→AGT)  csrD ← targeting factor for csrBC sRNA degradation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,396,0820GT99.7% 677.0 / ‑6.1 212R204S (CGT→AGT) csrDtargeting factor for csrBC sRNA degradation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (110/101);  minor base A (1/0);  total (111/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

ACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAG  >  CP009273/3395937‑3396224
                                                                                                                                                 |                                                                                                                                              
aCTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGGTGTGTTGTTCGCGTGCGTTCTGTATTTCACTaa                                                                                                                                              >  2:3141453/1‑148 (MQ=255)
aCTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTaa                                                                                                                                              >  2:1682561/1‑148 (MQ=255)
aCTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTaa                                                                                                                                              >  2:3172130/1‑148 (MQ=255)
 cTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAag                                                                                                                                             >  1:1641602/1‑148 (MQ=255)
       ccTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGt                                                                                                                                       <  1:2226999/148‑1 (MQ=255)
       ccTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGt                                                                                                                                       >  1:3461992/1‑148 (MQ=255)
        cTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTa                                                                                                                                      <  1:2704596/148‑1 (MQ=255)
         tGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTCTTTAGGCCCGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCACGGTATCAAGAAGGCGGTGTTTTTCGAGGGCGTTCTGAATATAAAAAAGCACCGTAt                                                                                                                                     >  1:1908175/1‑148 (MQ=255)
         tGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTAt                                                                                                                                     <  1:2650193/148‑1 (MQ=255)
          gATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATc                                                                                                                                    >  2:1486163/1‑148 (MQ=255)
          gATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATc                                                                                                                                    <  2:3121865/148‑1 (MQ=255)
            tCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCa                                                                                                                                  >  2:714110/1‑148 (MQ=255)
            tCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCa                                                                                                                                  <  1:2331418/148‑1 (MQ=255)
            tCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGGTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGGTGTTCGCGTGCGTTCTGAATTTCACTAATCAGCGTATCCa                                                                                                                                  >  1:2786203/1‑148 (MQ=255)
             cTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAg                                                                                                                                 <  1:1001251/148‑1 (MQ=255)
                 cAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTTGTGCCGGTTTTCACGTTCTGGGGGGCCTAANAGCGGATCAGCGTATAAAGACCGGTGGGGTGTTAGCGTGCGGTCTGAATTTCACTAATCAGCGTATCCAGCGCa                                                                                                                             >  1:1812039/1‑148 (MQ=255)
                     aaCGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCActgc                                                                                                                         <  2:1795170/148‑1 (MQ=255)
                      aCGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCActgct                                                                                                                        <  1:3413178/148‑1 (MQ=255)
                      aCGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATAAAGACGGCTGTGTTGTTCGCGTGCGGTCTGAATTTCACTAAGCAGCGTATACAGCGCActgct                                                                                                                        >  1:1832263/1‑148 (MQ=255)
                          tGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGtt                                                                                                                    <  1:727037/148‑1 (MQ=255)
                          tGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTAACTAAGCAGCGTATACAGCGCACTGCTGGtt                                                                                                                    >  1:3110593/1‑148 (MQ=255)
                            cTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCt                                                                                                                  <  2:3410772/148‑1 (MQ=255)
                               aCTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTggg                                                                                                               >  1:240934/1‑148 (MQ=255)
                                  gATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcgg                                                                                                            <  2:233691/148‑1 (MQ=255)
                                  gATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcgg                                                                                                            <  2:237829/148‑1 (MQ=255)
                                  gATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTAACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcgg                                                                                                            >  1:3164324/1‑148 (MQ=255)
                                     tATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATAAAGACGGCTGTGTTGTTAGCGGGCGTTCTGAATTTCACTAATCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCa                                                                                                         >  1:1927315/1‑148 (MQ=255)
                                          aaaaaGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCAt                                                                                                    >  2:869246/1‑148 (MQ=255)
                                           aaaaGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATa                                                                                                   >  2:1157784/1‑148 (MQ=255)
                                            aaaGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAg                                                                                                  <  1:2200281/148‑1 (MQ=255)
                                             aaGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCGGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGa                                                                                                 >  2:2036191/1‑148 (MQ=255)
                                              agagTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGAt                                                                                                <  2:1235738/148‑1 (MQ=255)
                                              agagTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGAt                                                                                                <  2:93353/148‑1 (MQ=255)
                                              agagTCGGTTATTGAGGCCGGTTGTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGAt                                                                                                <  2:1064039/148‑1 (MQ=255)
                                               gagTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATg                                                                                               <  2:2550166/148‑1 (MQ=255)
                                                 gTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGt                                                                                             >  2:1803475/1‑148 (MQ=255)
                                                  tCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGtt                                                                                            <  2:700682/148‑1 (MQ=255)
                                                  tCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGtt                                                                                            >  1:60327/1‑148 (MQ=255)
                                                   cGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTc                                                                                           >  2:301741/1‑148 (MQ=255)
                                                      ttATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCa                                                                                        >  2:60765/1‑148 (MQ=255)
                                                      ttATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCa                                                                                        >  2:171902/1‑148 (MQ=255)
                                                       tATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCaa                                                                                       >  1:2710485/1‑148 (MQ=255)
                                                       tATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCaa                                                                                       >  2:1396501/1‑148 (MQ=255)
                                                       tATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCaa                                                                                       >  1:1375414/1‑148 (MQ=255)
                                                        aTTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAac                                                                                      <  2:3201211/148‑1 (MQ=255)
                                                          tGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAacac                                                                                    <  1:939270/148‑1 (MQ=255)
                                                            aGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACAt                                                                                  <  1:3184672/148‑1 (MQ=255)
                                                            aGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACAt                                                                                  <  2:431268/148‑1 (MQ=255)
                                                            aGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTAACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACAt                                                                                  >  2:468072/1‑148 (MQ=255)
                                                             ggCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACAtt                                                                                 <  1:3532758/148‑1 (MQ=255)
                                                             ggCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACAtt                                                                                 <  1:28571/148‑1 (MQ=255)
                                                                cGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTaga                                                                              >  2:2834234/1‑148 (MQ=255)
                                                                      tCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACg                                                                        <  2:2939376/148‑1 (MQ=255)
                                                                      tCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACg                                                                        <  1:3349330/148‑1 (MQ=255)
                                                                        aCGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCt                                                                      <  2:1791347/148‑1 (MQ=255)
                                                                           tCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCAc                                                                   >  1:676046/1‑148 (MQ=255)
                                                                            ccTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCAcc                                                                  >  2:1091609/1‑148 (MQ=255)
                                                                            ccTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCAcc                                                                  >  1:29554/1‑148 (MQ=255)
                                                                              tGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGt                                                                >  1:1331787/1‑148 (MQ=255)
                                                                               gggCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGtt                                                               >  1:517472/1‑148 (MQ=255)
                                                                                 gcggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTa                                                             <  2:3313234/148‑1 (MQ=255)
                                                                                 gcggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTa                                                             <  2:3313482/148‑1 (MQ=255)
                                                                                    gcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGa                                                          <  2:1982380/148‑1 (MQ=255)
                                                                                     cATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGAt                                                         <  1:2174568/148‑1 (MQ=255)
                                                                                         agagCGTATCATCGTATCAAGACGGCTGTGTTGTTCGAGTGAGTTCTTAATTTCACTAAGGAGCGTATACAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACgt                                                     <  2:1908175/148‑2 (MQ=255)
                                                                                         agagCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGa                                                     <  1:2545688/148‑1 (MQ=255)
                                                                                          gagCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAg                                                    <  2:2635661/148‑1 (MQ=255)
                                                                                           agCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGt                                                   >  2:3555498/1‑148 (MQ=255)
                                                                                            gCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTa                                                  >  2:751183/1‑148 (MQ=255)
                                                                                             cGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAg                                                 <  2:29554/148‑1 (MQ=255)
                                                                                              ggATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGc                                                <  2:363424/148‑1 (MQ=255)
                                                                                               gATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGcc                                               >  2:299398/1‑148 (MQ=255)
                                                                                                   aGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCggg                                           <  1:1258379/148‑1 (MQ=255)
                                                                                                     cGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGtt                                         >  1:963776/1‑148 (MQ=255)
                                                                                                       tATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTc                                       >  1:805276/1‑148 (MQ=255)
                                                                                                       tATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTc                                       >  1:804118/1‑148 (MQ=255)
                                                                                                       tATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTc                                       <  1:2148897/148‑1 (MQ=255)
                                                                                                       tATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTc                                       >  1:2376586/1‑148 (MQ=255)
                                                                                                         tCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCa                                     <  1:869246/148‑1 (MQ=255)
                                                                                                               cGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAAtt                               <  1:171902/148‑1 (MQ=255)
                                                                                                                ggCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTc                              <  2:2762125/148‑1 (MQ=255)
                                                                                                                    gtgtTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACTCATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGc                          <  2:1053119/148‑1 (MQ=255)
                                                                                                                     tgttgtTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGcc                         >  1:1066209/1‑148 (MQ=255)
                                                                                                                      gttgttCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGccc                        >  1:1633778/1‑148 (MQ=255)
                                                                                                                           tCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCaa                   >  1:3506451/1‑148 (MQ=255)
                                                                                                                           tCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCaa                   >  1:1220556/1‑148 (MQ=255)
                                                                                                                           tCGCGTGCGTTCTGAATTTAACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCaa                   <  1:468072/148‑1 (MQ=255)
                                                                                                                            cgcgTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAg                  >  2:638159/1‑148 (MQ=255)
                                                                                                                             gcgtgcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGt                 <  2:1797158/148‑1 (MQ=255)
                                                                                                                                 gcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGcc             >  1:770827/1‑148 (MQ=255)
                                                                                                                                    ttCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGtt          >  1:1781611/1‑148 (MQ=255)
                                                                                                                                     tCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTg         >  2:1241549/1‑148 (MQ=255)
                                                                                                                                       tGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTa       <  2:1639264/148‑1 (MQ=255)
                                                                                                                                         aaTTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAc     >  2:1680892/1‑148 (MQ=255)
                                                                                                                                          aTTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAcc    <  2:1462499/148‑1 (MQ=255)
                                                                                                                                          aTTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAcc    <  2:1442397/148‑1 (MQ=255)
                                                                                                                                           tttCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCa   <  2:1281390/148‑1 (MQ=255)
                                                                                                                                           tttCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCa   >  2:417351/1‑148 (MQ=255)
                                                                                                                                           tttCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCa   >  2:2209912/1‑148 (MQ=255)
                                                                                                                                            ttCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAg  >  2:3466142/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
ACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAG  >  CP009273/3395937‑3396224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑