Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 279,334 C→A 100% I141I (ATC→ATA)  yagF → CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273279,3340CA95.2% 545.7 / 17.8 190I141I (ATC→ATA) yagFCP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/9);  new base A (69/112);  total (69/121)
Fisher's exact test for biased strand distribution p-value = 2.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 191 total aligned reads displayed.

CGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGG  >  CP009273/279187‑279471
                                                                                                                                                   |                                                                                                                                         
   cATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgtg                                                                                                                                        <  2:1485191/148‑1 (MQ=255)
   cATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgtg                                                                                                                                        <  2:2374581/148‑1 (MQ=255)
     tgcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTTGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGtt                                                                                                                                      >  1:2107723/1‑148 (MQ=255)
      gcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCTCAGGGCACGCACGGTATGTTCGATGCCCTGCCGTACCGCAACGACGAGGCGATAGTGttt                                                                                                                                     <  2:2792367/148‑1 (MQ=255)
      gcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGttt                                                                                                                                     <  2:311256/148‑1 (MQ=255)
      gcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGttt                                                                                                                                     >  2:2316057/1‑148 (MQ=255)
        agatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcg                                                                                                                                   <  1:3298365/148‑1 (MQ=255)
              agGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCt                                                                                                                             <  2:445953/148‑1 (MQ=255)
              agGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCt                                                                                                                             <  2:2483821/148‑1 (MQ=255)
                 cggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGAt                                                                                                                          <  2:3543915/148‑1 (MQ=255)
                  ggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATc                                                                                                                         <  1:3259329/148‑1 (MQ=255)
                   gcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATcc                                                                                                                        >  1:1538124/1‑148 (MQ=255)
                    cggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCg                                                                                                                       <  2:2548798/148‑1 (MQ=255)
                      gcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCt                                                                                                                     >  1:1256814/1‑148 (MQ=255)
                       cgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTc                                                                                                                    <  1:1917582/148‑1 (MQ=255)
                       cgAAGGAGATCACCCGCAATGGCGGGATCCCGGTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTc                                                                                                                    <  1:1710357/148‑1 (MQ=255)
                        gAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTcc                                                                                                                   <  2:1965148/148‑1 (MQ=255)
                         aaGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTccc                                                                                                                  <  1:233666/148‑1 (MQ=255)
                         aaGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTccc                                                                                                                  >  2:751006/1‑148 (MQ=255)
                         aaGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGAGAGTGTTTCGCCGCCTGATCCGCTccc                                                                                                                  >  2:1658046/1‑148 (MQ=255)
                           ggAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTg                                                                                                                >  1:2523681/1‑148 (MQ=255)
                             agaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGcc                                                                                                              >  1:1321632/1‑148 (MQ=255)
                               aTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGa                                                                                                            >  2:2724001/1‑148 (MQ=255)
                               aTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGa                                                                                                            >  1:141782/1‑148 (MQ=255)
                                 cACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAcg                                                                                                          >  1:2750441/1‑148 (MQ=255)
                                    ccGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACgcgg                                                                                                       <  1:1453059/148‑1 (MQ=255)
                                         aTGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCggcgggc                                                                                                  <  2:1979980/148‑1 (MQ=255)
                                         aTGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCggcgggc                                                                                                  <  2:2989026/148‑1 (MQ=255)
                                           ggCGGGATCCCGTTCGCGGCCTTCGTCCGCGATCCGTGCGACGGGCGCTCGCAGGGCCCGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGgcgggcgg                                                                                                >  2:3259124/1‑148 (MQ=255)
                                           ggCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGgcgggcgg                                                                                                >  2:3188511/1‑148 (MQ=255)
                                           ggCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGGATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGgcgggcgg                                                                                                >  2:626844/1‑148 (MQ=255)
                                            gCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGt                                                                                               <  1:842074/148‑1 (MQ=255)
                                               ggATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGAt                                                                                            <  2:2479331/148‑1 (MQ=255)
                                               ggATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGAt                                                                                            <  2:2166365/148‑1 (MQ=255)
                                                gATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATc                                                                                           >  1:1869949/1‑148 (MQ=255)
                                                gATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATc                                                                                           <  1:1953618/148‑1 (MQ=255)
                                                 aTCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCg                                                                                          >  2:3259000/1‑148 (MQ=255)
                                                  tCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCgg                                                                                         <  1:1173070/148‑1 (MQ=255)
                                                    ccGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCg                                                                                       <  1:507438/148‑1 (MQ=255)
                                                    ccGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCg                                                                                       <  2:1352268/148‑1 (MQ=255)
                                                     cGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGt                                                                                      >  1:872407/1‑148 (MQ=255)
                                                     cGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGt                                                                                      <  2:127095/148‑1 (MQ=255)
                                                        tCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGc                                                                                   >  1:2965490/1‑148 (MQ=255)
                                                           cgGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGAc                                                                                <  1:294348/148‑1 (MQ=255)
                                                              ccTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTg                                                                             <  1:3202551/148‑1 (MQ=255)
                                                               cTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGc                                                                            >  1:1118230/1‑148 (MQ=255)
                                                                ttCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCg                                                                           <  2:2976599/148‑1 (MQ=255)
                                                                ttCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCg                                                                           <  2:2085994/148‑1 (MQ=255)
                                                                ttCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCg                                                                           <  2:633416/148‑1 (MQ=255)
                                                                 tCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGa                                                                          <  2:859538/148‑1 (MQ=255)
                                                                     cAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                      >  2:2828022/1‑148 (MQ=255)
                                                                     cAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                      <  1:301240/148‑1 (MQ=255)
                                                                     cAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                      <  1:3060044/148‑1 (MQ=255)
                                                                     cAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                      >  2:1974313/1‑148 (MQ=255)
                                                                       gCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAgg                                                                    <  2:3142680/148‑1 (MQ=255)
                                                                        cGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAggg                                                                   <  2:1869949/148‑1 (MQ=255)
                                                                        cGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAggg                                                                   <  1:1722550/148‑1 (MQ=255)
                                                                        cGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAggg                                                                   <  2:644237/148‑1 (MQ=255)
                                                                         gATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGc                                                                  <  2:50660/148‑1 (MQ=255)
                                                                           tCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAACGGGCTg                                                                >  2:2003583/1‑148 (MQ=255)
                                                                           tCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTg                                                                >  1:3229928/1‑148 (MQ=255)
                                                                           tCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTg                                                                >  1:1094795/1‑148 (MQ=255)
                                                                           tCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTg                                                                >  1:2873867/1‑148 (MQ=255)
                                                                            ccGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGc                                                               >  1:788714/1‑148 (MQ=255)
                                                                                gCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGc                                                           <  2:2756973/148‑1 (MQ=255)
                                                                                gCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGc                                                           <  2:3322478/148‑1 (MQ=255)
                                                                                  gACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGcca                                                         <  1:2203209/148‑1 (MQ=255)
                                                                                      ggCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAt                                                     >  1:483496/1‑148 (MQ=255)
                                                                                      ggCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCAt                                                     >  2:1086653/1‑148 (MQ=255)
                                                                                       gcgcTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATg                                                    <  2:1321632/148‑1 (MQ=255)
                                                                                          cTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGAtt                                                 <  2:2604021/148‑1 (MQ=255)
                                                                                          cTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGAtt                                                 <  2:3103118/148‑1 (MQ=255)
                                                                                           tCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTg                                                <  1:2234173/148‑1 (MQ=255)
                                                                                              cAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTgcgc                                             <  1:2503966/148‑1 (MQ=255)
                                                                                               aGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATATAGGGCTGCCCGCCACCATGATTGCGCt                                            <  2:978778/148‑1 (MQ=255)
                                                                                               aGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCt                                            <  2:3157632/148‑1 (MQ=255)
                                                                                                  gcacgcacGGTATGTTTGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGTGCTGGc                                         <  2:2107723/148‑1 (MQ=255)
                                                                                                  gcacgcacGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGc                                         <  2:1002068/148‑1 (MQ=255)
                                                                                                       cacgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGa                                    <  1:1658046/148‑1 (MQ=255)
                                                                                                        acgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGAt                                   <  2:574295/148‑1 (MQ=255)
                                                                                                         cgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATg                                  <  1:2449208/148‑1 (MQ=255)
                                                                                                           gTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCTGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCa                                <  2:1691425/148‑1 (MQ=255)
                                                                                                              tGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGa                             <  1:1588196/148‑1 (MQ=255)
                                                                                                              tGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGa                             >  1:591927/1‑148 (MQ=255)
                                                                                                                ttCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGAcc                           <  2:871018/148‑1 (MQ=255)
                                                                                                                 tCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCAGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCt                          <  1:2315130/148‑1 (MQ=255)
                                                                                                                   gcttCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGGCGCGATGCACGACCTGc                        <  2:926087/146‑1 (MQ=255)
                                                                                                                          tGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTAt                 >  1:2726003/1‑148 (MQ=255)
                                                                                                                           gCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTAtt                <  2:2585350/148‑1 (MQ=255)
                                                                                                                           gCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTAtt                >  1:1962601/1‑148 (MQ=255)
                                                                                                                             cGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCt              <  2:1091029/148‑1 (MQ=255)
                                                                                                                             cGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCt              <  2:3451063/148‑1 (MQ=255)
                                                                                                                               tACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTgg            <  2:3492332/148‑1 (MQ=255)
                                                                                                                               tACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCCTGATTGCGCTGGCCGCGATGCCCGACCTGCCGACTATTCTgg            >  2:1963727/1‑148 (MQ=255)
                                                                                                                               tACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTgg            <  2:1094795/148‑1 (MQ=255)
                                                                                                                               tACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTgg            <  2:2873867/148‑1 (MQ=255)
                                                                                                                                    cAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcg       <  2:789140/148‑1 (MQ=255)
                                                                                                                                       cgacgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGAGCGGCGTAGCGACCTGCGAGAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACGATGCTGGTGCcgggc    >  2:3558747/1‑148 (MQ=255)
                                                                                                                                         acgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggcgg  >  1:3484991/1‑148 (MQ=255)
                                                                                                                                         acgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGAGTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggcgg  <  2:2254024/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
CGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGG  >  CP009273/279187‑279471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑