Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,486,738 A→T 28.3% Q271L (CAA→CTA)  tsgA → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,486,7380AT28.3% 337.9 / 167.6 201Q271L (CAA→CTA) tsgAputative transporter
Reads supporting (aligned to +/- strand):  ref base A (61/83);  new base T (23/34);  total (84/117)
Fisher's exact test for biased strand distribution p-value = 8.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 204 total aligned reads displayed.

AGTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCAC  >  CP009273/3486591‑3486884
                                                                                                                                                   |                                                                                                                                                  
 gTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCaa                                                                                                                                                   <  2:853874/148‑1 (MQ=255)
   tAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACg                                                                                                                                                 >  2:1006463/1‑148 (MQ=255)
    aGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGc                                                                                                                                                <  2:892107/148‑1 (MQ=255)
     ggTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCa                                                                                                                                               >  1:642992/1‑148 (MQ=255)
      gTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCAt                                                                                                                                              >  2:1565122/1‑148 (MQ=255)
       ttttATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCAtt                                                                                                                                             <  1:1465790/148‑1 (MQ=255)
       ttttATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGAATGAGCCTGAACGACTCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCAtt                                                                                                                                             <  1:2856377/148‑1 (MQ=255)
          tATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTg                                                                                                                                          <  2:2987377/148‑1 (MQ=255)
          tATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTg                                                                                                                                          >  1:1886101/1‑148 (MQ=255)
            tctcCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGAc                                                                                                                                        >  2:2212787/1‑148 (MQ=255)
               ccTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGt                                                                                                                                     >  1:1829348/1‑148 (MQ=255)
                cTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTa                                                                                                                                    <  2:1910952/148‑1 (MQ=255)
                cTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTa                                                                                                                                    <  1:3161251/148‑1 (MQ=255)
                 tGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTAc                                                                                                                                   >  1:1780253/1‑148 (MQ=255)
                 tGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTAc                                                                                                                                   >  1:539385/1‑148 (MQ=255)
                   ggTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTActg                                                                                                                                 >  2:1399529/1‑148 (MQ=255)
                    gTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTActgg                                                                                                                                <  2:1356403/148‑1 (MQ=255)
                      gCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTActggct                                                                                                                              <  1:3346874/148‑1 (MQ=255)
                       ccTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTActggctg                                                                                                                             <  1:2710392/148‑1 (MQ=255)
                       ccTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTActggctg                                                                                                                             >  1:3447090/1‑148 (MQ=255)
                             tATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTgg                                                                                                                       <  2:704749/148‑1 (MQ=255)
                             tATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTgg                                                                                                                       <  1:2510750/148‑1 (MQ=255)
                              aTGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGc                                                                                                                      <  1:159891/148‑1 (MQ=255)
                                gCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTg                                                                                                                    <  1:1471644/148‑1 (MQ=255)
                                   aaaGGTCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGa                                                                                                                 >  1:3224064/1‑148 (MQ=255)
                                   aaaGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGa                                                                                                                 <  2:263205/148‑1 (MQ=255)
                                   aaaGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGa                                                                                                                 <  1:1694660/148‑1 (MQ=255)
                                   aaaGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGa                                                                                                                 <  1:1694476/148‑1 (MQ=255)
                                     aGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGAtt                                                                                                               <  1:2658962/148‑1 (MQ=255)
                                        ccTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTg                                                                                                            <  1:2464996/148‑1 (MQ=255)
                                        ccTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTg                                                                                                            <  1:987165/148‑1 (MQ=255)
                                         cTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGa                                                                                                           <  2:1329725/148‑1 (MQ=255)
                                           gggCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATg                                                                                                         <  2:1828202/148‑1 (MQ=255)
                                            ggCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGt                                                                                                        >  2:3550733/1‑148 (MQ=255)
                                            ggCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGt                                                                                                        >  1:725686/1‑148 (MQ=255)
                                                 gAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTc                                                                                                   <  1:480438/148‑1 (MQ=255)
                                                 gAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTc                                                                                                   <  2:3107875/148‑1 (MQ=255)
                                                  aGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCt                                                                                                  <  2:2375172/148‑1 (MQ=255)
                                                   gCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCtt                                                                                                 <  1:3037986/148‑1 (MQ=255)
                                                   gCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCtt                                                                                                 <  1:1262234/148‑1 (MQ=255)
                                                    ccTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCttt                                                                                                <  2:1054883/148‑1 (MQ=255)
                                                     cTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTa                                                                                               <  2:1519450/148‑1 (MQ=255)
                                                      tGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTaa                                                                                              <  2:2466454/148‑1 (MQ=255)
                                                       gAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAac                                                                                             >  2:2238714/1‑148 (MQ=255)
                                                        aaCGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAaca                                                                                            >  2:3300973/1‑148 (MQ=255)
                                                          cgacgCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACAcc                                                                                          >  2:1748492/1‑148 (MQ=255)
                                                          cgacgCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACAcc                                                                                          >  2:3510688/1‑148 (MQ=255)
                                                          cgacgCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACAcc                                                                                          >  2:3154518/1‑148 (MQ=255)
                                                                 ggCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAacac                                                                                   <  2:1370693/148‑1 (MQ=255)
                                                                 ggCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAacac                                                                                   <  2:2127498/148‑1 (MQ=255)
                                                                   cACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCa                                                                                 >  1:857774/1‑148 (MQ=255)
                                                                    aCGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAg                                                                                <  1:2289656/148‑1 (MQ=255)
                                                                         ggTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACat                                                                           <  1:1399529/148‑1 (MQ=255)
                                                                           tGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACatat                                                                         <  1:2372768/148‑1 (MQ=255)
                                                                               tAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCg                                                                     <  1:1062551/148‑1 (MQ=255)
                                                                                 aCTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTg                                                                   <  1:209614/148‑1 (MQ=255)
                                                                                     cTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCa                                                               <  2:2688298/148‑1 (MQ=255)
                                                                                     cTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCa                                                               <  2:405425/148‑1 (MQ=255)
                                                                                        gATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAAtt                                                            <  2:201896/148‑1 (MQ=255)
                                                                                         aTGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATtc                                                           <  2:535628/148‑1 (MQ=255)
                                                                                           gTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATtctc                                                         >  2:1163242/1‑148 (MQ=255)
                                                                                            tCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTATCg                                                        >  1:1681833/1‑148 (MQ=255)
                                                                                               tACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGctc                                                     >  2:3257096/1‑148 (MQ=255)
                                                                                                aCATGGTCGGCATGTGGGCGTTCAGCTTTATTATTAGCTTCTTTGATTTGCAACGCATTCTGACCGTACAGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACAAATGGCGTGGTCAAATCTCGctct                                                    >  1:2402978/1‑148 (MQ=255)
                                                                                                   tGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTggg                                                 <  1:3389392/148‑1 (MQ=255)
                                                                                                   tGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTGTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTATTATGTACGTCTTTTAAAACGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTggg                                                 >  1:1301571/1‑148 (MQ=255)
                                                                                                    ggTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGc                                                <  1:2212787/148‑1 (MQ=255)
                                                                                                     gTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGct                                               >  2:2969015/1‑148 (MQ=255)
                                                                                                        ggCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGcttct                                            <  2:3447090/148‑1 (MQ=255)
                                                                                                         gCATGTGGGCGTTGAGATTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCCGCACATATGGCGTGGTCAATTCTCGCTCTGGGcttctt                                           <  1:1475138/148‑1 (MQ=255)
                                                                                                         gCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGcttctt                                           <  1:695177/148‑1 (MQ=255)
                                                                                                         gCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGcttctt                                           <  1:894334/148‑1 (MQ=255)
                                                                                                          cATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCttcttc                                          <  1:1263007/148‑1 (MQ=255)
                                                                                                            tgtgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTtctc                                        <  2:2831474/148‑1 (MQ=255)
                                                                                                            tgtgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTtctc                                        <  2:3067940/148‑1 (MQ=255)
                                                                                                            tgtgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTtctc                                        <  2:3068167/148‑1 (MQ=255)
                                                                                                            tgtgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTtctc                                        <  2:2757518/148‑1 (MQ=255)
                                                                                                            tgtgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTtctc                                        <  2:1718868/148‑1 (MQ=255)
                                                                                                             gtgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTcc                                       >  2:3152829/1‑148 (MQ=255)
                                                                                                              tgGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTTCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCa                                      >  1:776472/1‑148 (MQ=255)
                                                                                                                ggCGGTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAgc                                    >  1:1863046/1‑148 (MQ=255)
                                                                                                                    ttCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGa                                >  2:814912/1‑148 (MQ=255)
                                                                                                                     tCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGAt                               <  2:1258023/148‑1 (MQ=255)
                                                                                                                     tCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGAt                               >  1:3043526/1‑148 (MQ=255)
                                                                                                                      cAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATc                              <  1:1163242/148‑1 (MQ=255)
                                                                                                                        gCTTTATTCTTCGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCta                            <  1:1564987/148‑1 (MQ=255)
                                                                                                                        gCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCta                            >  2:2708890/1‑148 (MQ=255)
                                                                                                                          tttATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCtata                          <  2:3206591/148‑1 (MQ=255)
                                                                                                                          tttATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCtata                          <  1:2952998/148‑1 (MQ=255)
                                                                                                                               tcttcGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATaccacc                     <  2:1886101/148‑1 (MQ=255)
                                                                                                                                 ttcGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACcat                   <  1:1746440/148‑1 (MQ=255)
                                                                                                                                   cGCTTCTTTGATTTGCTACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACcatca                 >  1:1213488/1‑148 (MQ=255)
                                                                                                                                          ttGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCt          <  2:3224063/148‑1 (MQ=255)
                                                                                                                                           tGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTg         >  2:440019/1‑148 (MQ=255)
                                                                                                                                             aTTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTggg       <  1:297897/148‑1 (MQ=255)
                                                                                                                                               ttGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGtt     >  2:1658027/1‑148 (MQ=255)
                                                                                                                                                 gCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGGTGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTca   <  1:368055/148‑1 (MQ=255)
                                                                                                                                                  cAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTcac  >  2:3085268/1‑148 (MQ=255)
                                                                                                                                                  cAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTcac  >  1:2074082/1‑148 (MQ=255)
                                                                                                                                                  cAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTcac  <  2:2931747/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGTTAGGTTTTATCTCCTGGGTGCCTGAGTATGCCAAAGGCCTGGGCATGAGCCTGAACGACGCGGGCACGCTGGTGAGTAACTTCTGGATGTCATACATGGTCGGCATGTGGGCGTTCAGCTTTATTCTTCGCTTCTTTGATTTGCAACGCATTCTGACCGTACTGGCTGGTCTGGCTGCGATTCTGATGTACGTCTTTAACACCGGAACACCAGCACATATGGCGTGGTCAATTCTCGCTCTGGGCTTCTTCTCCAGCGCGATCTATACCACCATCATCACCCTGGGTTCAC  >  CP009273/3486591‑3486884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑