Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,543,959 G→T 100% A625E (GCG→GAG)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,543,9590GT99.4% 571.0 / ‑4.2 195A625E (GCG→GAG) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (113/78);  total (117/78)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 197 total aligned reads displayed.

TTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC  >  CP009273/3543816‑3544101
                                                                                                                                               |                                                                                                                                              
tttCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCgg                                                                                                                                            >  2:3032157/1‑148 (MQ=255)
tttCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCgg                                                                                                                                            >  2:1509235/1‑148 (MQ=255)
 ttcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCggg                                                                                                                                           >  2:1602817/1‑148 (MQ=255)
  tcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGa                                                                                                                                          >  1:2265164/1‑148 (MQ=255)
  tcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGa                                                                                                                                          >  1:2395859/1‑148 (MQ=255)
   cttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGAt                                                                                                                                         <  2:2146391/148‑1 (MQ=255)
   cttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGAt                                                                                                                                         <  2:14667/148‑1 (MQ=255)
    ttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATc                                                                                                                                        >  2:3145605/1‑148 (MQ=255)
    ttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATc                                                                                                                                        >  1:2907837/1‑148 (MQ=255)
    ttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTAGGAGATATCCGCCTCCGGGATc                                                                                                                                        >  1:1928585/1‑148 (MQ=255)
      cACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAg                                                                                                                                      <  2:3317269/148‑1 (MQ=255)
        ccGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtt                                                                                                                                    >  1:150491/1‑148 (MQ=255)
        ccGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtt                                                                                                                                    >  1:2750429/1‑148 (MQ=255)
        ccGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtt                                                                                                                                    >  2:1206520/1‑148 (MQ=255)
        ccGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtt                                                                                                                                    >  1:150591/1‑148 (MQ=255)
        ccGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtt                                                                                                                                    >  2:2368010/1‑148 (MQ=255)
           aCTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttttt                                                                                                                                 >  2:1079278/1‑148 (MQ=255)
            cTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTc                                                                                                                                <  2:1536223/148‑1 (MQ=255)
               tctcGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgc                                                                                                                             >  2:3433206/1‑148 (MQ=255)
                ctcGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgcc                                                                                                                            >  2:2303281/1‑148 (MQ=255)
                   ggCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgccgcc                                                                                                                         <  2:2231674/148‑1 (MQ=255)
                    gCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAAGTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgccgccg                                                                                                                        >  1:3001955/1‑148 (MQ=255)
                     cGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGa                                                                                                                       >  2:148104/1‑148 (MQ=255)
                      gATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGaa                                                                                                                      >  1:795222/1‑148 (MQ=255)
                       aTTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGaaa                                                                                                                     <  1:3537368/148‑1 (MQ=255)
                        tttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAc                                                                                                                    >  1:411523/1‑148 (MQ=255)
                         ttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACg                                                                                                                   >  1:3413899/1‑148 (MQ=255)
                            aaCGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaa                                                                                                                >  1:2360926/1‑148 (MQ=255)
                            aaCGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaa                                                                                                                <  1:2812087/148‑1 (MQ=255)
                              cGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaata                                                                                                              <  1:487440/148‑1 (MQ=255)
                               gTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaataa                                                                                                             >  1:752273/1‑148 (MQ=255)
                                    ccccATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCgg                                                                                                        <  1:1490307/148‑1 (MQ=255)
                                      ccATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCa                                                                                                      <  1:1364956/148‑1 (MQ=255)
                                         tCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGa                                                                                                   >  1:2217619/1‑148 (MQ=255)
                                         tCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGa                                                                                                   >  1:2217537/1‑148 (MQ=255)
                                          ccAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGaa                                                                                                  >  1:3242204/1‑148 (MQ=255)
                                            aGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAaca                                                                                                <  1:3562113/148‑1 (MQ=255)
                                              cGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAAcacc                                                                                              >  2:2277183/1‑148 (MQ=255)
                                                tACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAAcaccac                                                                                            >  2:2974754/1‑148 (MQ=255)
                                                      cAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTTTTAGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACCCAAAAATACGGCAGGAACACCACCTTCaa                                                                                      >  1:3336453/1‑148 (MQ=255)
                                                      cAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCCGGCTCAGTTTTTACGCCGCCGAAACGCAATAAAACGGCAGGAACACCCCCATCaa                                                                                      >  1:1541453/1‑148 (MQ=255)
                                                       aGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCGTCAAc                                                                                     >  1:1065308/1‑148 (MQ=255)
                                                         tAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACtt                                                                                   <  2:2252964/148‑1 (MQ=255)
                                                         tAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACtt                                                                                   <  1:1079278/148‑1 (MQ=255)
                                                           aGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTAt                                                                                 <  2:3508142/148‑1 (MQ=255)
                                                            gcgcACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATc                                                                                <  1:786431/148‑1 (MQ=255)
                                                                  cATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAAc                                                                          >  2:217044/1‑148 (MQ=255)
                                                                   aTTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAAcc                                                                         <  1:1095957/148‑1 (MQ=255)
                                                                   aTTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTAACTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATACGGCAGGAACACCACCATCAAATTATCGCCaaac                                                                         >  1:1607815/1‑146 (MQ=255)
                                                                    ttGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCa                                                                        >  2:1127588/1‑148 (MQ=255)
                                                                     tGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAg                                                                       >  2:3199774/1‑148 (MQ=255)
                                                                     tGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAg                                                                       <  1:1877645/148‑1 (MQ=255)
                                                                      gAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGc                                                                      <  1:545956/148‑1 (MQ=255)
                                                                            cAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCCCCGAAACGCAAAAATCCGGCAGGAACACCACCATCAACATATCGCCAACCAGCGGATCg                                                                >  1:739587/1‑148 (MQ=255)
                                                                               tttCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACATATCGCCAACCAGCGGATCgttg                                                             >  2:1950008/1‑148 (MQ=255)
                                                                               tttCATATTGCCGGTACCGGAAGCTTCTTTAACTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAAACCGGCAGGAACACCACCATCAACATATCGCCAACCAGCGGATCgttg                                                             >  1:1323180/1‑148 (MQ=255)
                                                                                ttCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgttgt                                                            >  1:562311/1‑148 (MQ=255)
                                                                                 tCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgttgtt                                                           <  2:1146622/148‑1 (MQ=255)
                                                                                 tCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgttgtt                                                           >  2:2741508/1‑148 (MQ=255)
                                                                                  cATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGttgttg                                                          <  2:2822196/148‑1 (MQ=255)
                                                                                    tatTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGAt                                                        <  2:590889/148‑1 (MQ=255)
                                                                                    tatTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGAt                                                        <  1:1507188/148‑1 (MQ=255)
                                                                                    tatTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTAATCGCCAACCACCGGATCGTTGTTGAt                                                        >  2:2877348/1‑148 (MQ=255)
                                                                                              aCCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGcc                                              >  2:2652062/1‑148 (MQ=255)
                                                                                               ccGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCa                                             <  1:3145605/148‑1 (MQ=255)
                                                                                                 ggAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACt                                           <  2:768562/148‑1 (MQ=255)
                                                                                                  gAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACtt                                          <  1:682953/148‑1 (MQ=255)
                                                                                                   aaGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACttt                                         <  2:147448/148‑1 (MQ=255)
                                                                                                   aaGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACttt                                         >  2:2307917/1‑148 (MQ=255)
                                                                                                   aaGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACttt                                         <  1:1165244/148‑1 (MQ=255)
                                                                                                   aaGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACttt                                         >  1:2919122/1‑148 (MQ=255)
                                                                                                        tcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGa                                    <  2:3084814/148‑1 (MQ=255)
                                                                                                        tcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGa                                    >  1:1334536/1‑148 (MQ=255)
                                                                                                        tcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGa                                    >  1:1767972/1‑148 (MQ=255)
                                                                                                           ttACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCg                                 >  1:2203844/1‑148 (MQ=255)
                                                                                                           ttACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCg                                 >  1:447819/1‑148 (MQ=255)
                                                                                                             aCCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCa                               >  1:3093875/1‑148 (MQ=255)
                                                                                                             aCCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTATTGATCACGTCAGCCACTTTGTTGATCGCa                               >  1:116214/1‑148 (MQ=255)
                                                                                                              ccTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCaa                              <  1:471754/148‑1 (MQ=255)
                                                                                                               cTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCaaa                             <  1:2904543/148‑1 (MQ=255)
                                                                                                                  cAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGat                          <  2:2512411/148‑1 (MQ=255)
                                                                                                                   aGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGata                         <  2:3379842/148‑1 (MQ=255)
                                                                                                                     tCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                       >  2:1405594/1‑148 (MQ=255)
                                                                                                                     tCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                       >  1:1659801/1‑148 (MQ=255)
                                                                                                                     tCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                       <  1:2088396/148‑1 (MQ=255)
                                                                                                                     tCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                       >  1:611905/1‑148 (MQ=255)
                                                                                                                     tCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                       >  2:2955409/1‑148 (MQ=255)
                                                                                                                      cGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataata                      >  2:964785/1‑148 (MQ=255)
                                                                                                                       gAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAatat                     <  1:451438/148‑1 (MQ=255)
                                                                                                                        aaaTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAtt                    <  1:1206520/148‑1 (MQ=255)
                                                                                                                         aaTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttc                   >  1:972067/1‑148 (MQ=255)
                                                                                                                          aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCTAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttct                  >  1:2805595/1‑148 (MQ=255)
                                                                                                                           tttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttctt                 >  2:1283884/1‑148 (MQ=255)
                                                                                                                           tttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttctt                 >  2:630777/1‑148 (MQ=255)
                                                                                                                               ttCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGcc             >  1:2573457/1‑148 (MQ=255)
                                                                                                                                     gaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtag       <  1:2990805/148‑1 (MQ=255)
                                                                                                                                      atatCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtagt      >  1:2471351/1‑148 (MQ=255)
                                                                                                                                         tCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGc   <  2:1659801/148‑1 (MQ=255)
                                                                                                                                         tCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGc   >  1:1057768/1‑148 (MQ=255)
                                                                                                                                         tCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGc   >  1:1057766/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                              
TTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC  >  CP009273/3543816‑3544101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑