Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 505,336 C→T 5.0% E500E (GAG→GAA)  copA ← copper transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273505,3360CT 5.0% 1301.3 / 34.6 356E500E (GAG→GAA) copAcopper transporter
Reads supporting (aligned to +/- strand):  ref base C (169/169);  new base T (12/6);  total (181/175)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

AATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGCCGCACT  >  CP009273/505187‑505485
                                                                                                                                                     |                                                                                                                                                     
 aTGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACt                                                                                                                                                      <  2:4314369/150‑1 (MQ=255)
    ccTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAg                                                                                                                                                   >  2:3460726/1‑150 (MQ=255)
     cTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgc                                                                                                                                                  >  2:4420456/1‑150 (MQ=255)
      tGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgcc                                                                                                                                                 <  2:2626760/150‑1 (MQ=255)
       gcgcTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgccg                                                                                                                                                <  2:1664015/150‑1 (MQ=255)
       gcgcTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgccg                                                                                                                                                <  2:3652472/150‑1 (MQ=255)
         gcTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgccgcc                                                                                                                                              >  1:4280316/1‑150 (MQ=255)
          cTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGccgccc                                                                                                                                             <  1:4566044/150‑1 (MQ=255)
           ttCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGccgcccg                                                                                                                                            <  1:4346174/150‑1 (MQ=255)
           ttCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGccgcccg                                                                                                                                            >  1:1980177/1‑150 (MQ=255)
            tcatcaACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGccgcccgc                                                                                                                                           >  2:3513654/1‑150 (MQ=255)
                 aaCATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGAc                                                                                                                                      <  1:1211338/150‑1 (MQ=255)
                   cATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGc                                                                                                                                    >  1:2933277/1‑150 (MQ=255)
                        gCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGaa                                                                                                                               >  2:3696124/1‑150 (MQ=255)
                              gTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATc                                                                                                                         <  1:1013843/150‑1 (MQ=255)
                                    aCTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGCCATc                                                                                                                   >  2:5368261/1‑150 (MQ=255)
                                     cTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCg                                                                                                                  >  1:408651/1‑150 (MQ=255)
                                      tGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCgg                                                                                                                 <  1:922138/150‑1 (MQ=255)
                                        cGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCg                                                                                                               >  1:5309224/1‑150 (MQ=255)
                                        cGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCg                                                                                                               <  1:4982726/150‑1 (MQ=255)
                                           cAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCg                                                                                                            >  2:47389/1‑150 (MQ=255)
                                           cAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCg                                                                                                            >  1:1657699/1‑150 (MQ=255)
                                            aaCCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGc                                                                                                           >  1:2551818/1‑150 (MQ=255)
                                             aCCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGcc                                                                                                          <  2:2032854/150‑1 (MQ=255)
                                              ccTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCa                                                                                                         <  2:3909880/150‑1 (MQ=255)
                                              ccTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCa                                                                                                         <  2:1212481/150‑1 (MQ=255)
                                              ccTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCa                                                                                                         <  1:4228702/150‑1 (MQ=255)
                                               cTGCGGCTTCCCTTCAGTCAGCGTCCCGGTGTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGcccg                                                                                                        >  1:468543/1‑148 (MQ=255)
                                                tGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGc                                                                                                       >  1:3424177/1‑150 (MQ=255)
                                                   ggCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGcccc                                                                                                    <  2:4809700/150‑1 (MQ=255)
                                                    gCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCa                                                                                                   <  1:763122/150‑1 (MQ=255)
                                                    gCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCa                                                                                                   <  1:4964983/150‑1 (MQ=255)
                                                    gCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCa                                                                                                   <  1:1501839/150‑1 (MQ=255)
                                                       tCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAgcg                                                                                                >  2:581874/1‑150 (MQ=255)
                                                        cccTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAgcgc                                                                                               <  2:1607585/150‑1 (MQ=255)
                                                         ccTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGca                                                                                              >  2:1116036/1‑150 (MQ=255)
                                                            tcagtcagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCacac                                                                                           <  1:4115799/150‑1 (MQ=255)
                                                              agtcagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACgg                                                                                         <  2:3107814/150‑1 (MQ=255)
                                                               gtcagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGa                                                                                        >  2:3010905/1‑150 (MQ=255)
                                                                 cagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACa                                                                                      >  1:2836322/1‑150 (MQ=255)
                                                                 cagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACa                                                                                      >  2:5437342/1‑150 (MQ=255)
                                                                  agCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCAGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAg                                                                                     >  2:3253913/1‑150 (MQ=255)
                                                                   gCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAgg                                                                                    <  2:2454546/150‑1 (MQ=255)
                                                                    cGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGc                                                                                   <  1:2626707/150‑1 (MQ=255)
                                                                    cGTACCGGTTTTATCGAACACTACAGTGTAGAGTGTTCTGGCGAGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCTACGCCTGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGc                                                                                   <  2:2983670/150‑1 (MQ=255)
                                                                     gTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCa                                                                                  <  1:2051089/150‑1 (MQ=255)
                                                                     gTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCa                                                                                  >  1:5097245/1‑150 (MQ=255)
                                                                     gTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCa                                                                                  <  1:3696140/150‑1 (MQ=255)
                                                                      tCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCaa                                                                                 >  2:4017717/1‑150 (MQ=255)
                                                                       cccGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCTCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCaat                                                                                >  1:1124935/1‑150 (MQ=255)
                                                                       cccGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCaat                                                                                <  2:1814348/150‑1 (MQ=255)
                                                                       cccGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCaat                                                                                <  1:4825974/150‑1 (MQ=255)
                                                                       cccGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGACCGCCCGACGCCGGAAATAATCGACAGCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCaat                                                                                >  2:4358484/1‑150 (MQ=255)
                                                                           gTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATc                                                                            <  2:2664468/150‑1 (MQ=255)
                                                                              ttATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGt                                                                         >  1:2033575/1‑150 (MQ=255)
                                                                               tATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTa                                                                        <  1:238651/150‑1 (MQ=255)
                                                                                aTCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTAc                                                                       <  2:1052841/150‑1 (MQ=255)
                                                                                 tCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTAcc                                                                      <  2:1980177/150‑1 (MQ=255)
                                                                                  cGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCg                                                                     >  2:761855/1‑150 (MQ=255)
                                                                                   gAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGCACAGGCAATAATCAGTACCgt                                                                    >  1:2791195/1‑150 (MQ=255)
                                                                                    aaCACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtg                                                                   <  1:654798/150‑1 (MQ=255)
                                                                                    aaCACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtg                                                                   >  2:1829038/1‑150 (MQ=255)
                                                                                    aaCACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtg                                                                   >  2:2700867/1‑150 (MQ=255)
                                                                                    aaCACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtg                                                                   >  1:3980613/1‑150 (MQ=255)
                                                                                      cacTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtggt                                                                 <  2:849745/150‑1 (MQ=255)
                                                                                         tACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGc                                                              >  1:1328172/1‑150 (MQ=255)
                                                                                               gtCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCCGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCAc                                                        >  1:3093044/1‑150 (MQ=255)
                                                                                               gtCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCAc                                                        <  2:219906/150‑1 (MQ=255)
                                                                                                tCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCAcc                                                       >  1:2112669/1‑150 (MQ=255)
                                                                                                    gtgtACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAggg                                                   <  2:4956354/150‑1 (MQ=255)
                                                                                                       tACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGtat                                                >  1:1254402/1‑150 (MQ=255)
                                                                                                        aCTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGtata                                               >  1:4643225/1‑150 (MQ=255)
                                                                                                         cTGGCGCGTTGCAGCGCGTCAGCGTCCAGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACCGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAg                                              >  2:2759959/1‑150 (MQ=255)
                                                                                                          tGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGa                                             >  1:2280667/1‑150 (MQ=255)
                                                                                                          tGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGa                                             >  2:9491/1‑150 (MQ=255)
                                                                                                          tGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGa                                             >  1:2671713/1‑150 (MQ=255)
                                                                                                           ggCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGAc                                            <  1:3239984/150‑1 (MQ=255)
                                                                                                              gcgTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAAt                                         <  1:1363784/150‑1 (MQ=255)
                                                                                                                gTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCt                                       <  1:2618705/150‑1 (MQ=255)
                                                                                                                gTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCt                                       >  2:4138456/1‑150 (MQ=255)
                                                                                                                gTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCt                                       <  2:5176870/150‑1 (MQ=255)
                                                                                                                gTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCt                                       <  1:1813302/150‑1 (MQ=255)
                                                                                                                  tGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGc                                     >  1:715661/1‑150 (MQ=255)
                                                                                                                        gcgTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGcc                               <  1:4852627/150‑1 (MQ=255)
                                                                                                                          gTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCgg                             >  1:2501968/1‑150 (MQ=255)
                                                                                                                          gTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCTATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCgg                             >  1:5372281/1‑150 (MQ=255)
                                                                                                                           tCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGa                            <  1:3379759/150‑1 (MQ=255)
                                                                                                                             aGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGAcc                          >  1:3524666/1‑150 (MQ=255)
                                                                                                                              gCGTCCCGCACCAGCACGACAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACCTCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCa                         <  2:1323380/150‑1 (MQ=255)
                                                                                                                                  cccGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGa                     >  2:5264646/1‑150 (MQ=255)
                                                                                                                                   ccGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGaa                    <  2:2551864/150‑1 (MQ=255)
                                                                                                                                     gCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAAt                  <  1:3460929/150‑1 (MQ=255)
                                                                                                                                      cACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATa                 >  2:3323238/1‑150 (MQ=255)
                                                                                                                                      cACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATa                 >  1:577538/1‑150 (MQ=255)
                                                                                                                                       aCCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATAc                <  2:3406467/150‑1 (MQ=255)
                                                                                                                                            cACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGAt           >  2:3071541/1‑150 (MQ=255)
                                                                                                                                               gCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGc        >  1:1769905/1‑150 (MQ=255)
                                                                                                                                                ccAAACTCAGCCGCCCGCCCGACGCCTGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACTATCTGCGGTGCCGGACGAAAGAAATACCAGATTGcc       <  2:1142260/150‑1 (MQ=255)
                                                                                                                                                ccAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGcc       <  2:2285178/150‑1 (MQ=255)
                                                                                                                                                ccAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGcc       <  2:5006524/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGCCGCACT  >  CP009273/505187‑505485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑