Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,122,159 G→T 6.9% A183S (GCG→TCG)  yceH → UPF0502 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,122,1590GT 6.9% 1084.7 / 45.6 286A183S (GCG→TCG) yceHUPF0502 family protein
Reads supporting (aligned to +/- strand):  ref base G (125/141);  new base T (8/12);  total (133/153)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

TGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGC  >  CP009273/1122010‑1122305
                                                                                                                                                     |                                                                                                                                                  
     tCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGtt                                                                                                                                               <  1:15855/150‑1 (MQ=255)
      cGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTg                                                                                                                                              >  2:2311876/1‑150 (MQ=255)
       gACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACTCGGTTGa                                                                                                                                             >  2:4565371/1‑150 (MQ=255)
       gACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGa                                                                                                                                             >  2:2355820/1‑150 (MQ=255)
          gCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACgg                                                                                                                                          <  1:4961733/150‑1 (MQ=255)
           cTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGt                                                                                                                                         >  2:628564/1‑150 (MQ=255)
                acaacTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGAttt                                                                                                                                    <  2:1083163/150‑1 (MQ=255)
                  aacTGGCAAACCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATACATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACTCGGTTGACGGTGATTTAc                                                                                                                                  <  1:1795665/150‑1 (MQ=255)
                    cTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACTCGGTTGACGGTGATTTACAg                                                                                                                                >  1:4662281/1‑150 (MQ=255)
                     tGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAgg                                                                                                                               >  2:3752849/1‑150 (MQ=255)
                      ggCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCACGCGAACCGGGTAAACGCTTAAAACTCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAAAGCGGTTGACGGTGATTTACAGGc                                                                                                                              <  2:4489078/150‑1 (MQ=255)
                        cAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGccc                                                                                                                            >  1:2244677/1‑150 (MQ=255)
                               cgAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGa                                                                                                                     >  2:2493695/1‑150 (MQ=255)
                               cgAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGa                                                                                                                     <  2:2104117/150‑1 (MQ=255)
                               cgAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGa                                                                                                                     <  2:142734/150‑1 (MQ=255)
                                gAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGaa                                                                                                                    <  1:3368545/150‑1 (MQ=255)
                                gAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGaa                                                                                                                    <  1:2393211/150‑1 (MQ=255)
                                gAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGaa                                                                                                                    >  2:3265443/1‑150 (MQ=255)
                                 aaGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAg                                                                                                                   >  1:901174/1‑150 (MQ=255)
                                   gATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGcc                                                                                                                 <  1:3638656/150‑1 (MQ=255)
                                    aTGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGccc                                                                                                                <  2:2030508/150‑1 (MQ=255)
                                    aTGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGccc                                                                                                                >  1:1043631/1‑150 (MQ=255)
                                     tGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCt                                                                                                               <  2:4697178/150‑1 (MQ=255)
                                     tGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCt                                                                                                               >  2:173935/1‑150 (MQ=255)
                                       gTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGGTCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTgg                                                                                                             >  2:775301/1‑150 (MQ=255)
                                       gTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATAAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTgg                                                                                                             >  2:5111255/1‑150 (MQ=255)
                                        tCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAACCGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGCAGCCCTGGa                                                                                                            <  1:1521591/150‑1 (MQ=255)
                                           ttttGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAAt                                                                                                         >  1:447709/1‑150 (MQ=255)
                                            tttGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATc                                                                                                        <  1:1427678/150‑1 (MQ=255)
                                              tgtgGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGa                                                                                                      <  1:2697349/150‑1 (MQ=255)
                                                tggtgCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACTGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAg                                                                                                    <  2:1115061/150‑1 (MQ=255)
                                                 ggtgCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGt                                                                                                   <  1:3274254/150‑1 (MQ=255)
                                                 ggtgCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGt                                                                                                   <  1:4145608/150‑1 (MQ=255)
                                                  gtgCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTg                                                                                                  >  2:2437988/1‑150 (MQ=255)
                                                    gCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAATGGc                                                                                                <  2:1840746/150‑1 (MQ=255)
                                                          ggCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACt                                                                                          <  2:2588593/150‑1 (MQ=255)
                                                           gCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTg                                                                                         >  2:2742172/1‑150 (MQ=255)
                                                           gCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTg                                                                                         <  1:1207398/150‑1 (MQ=255)
                                                            cccGCGTACCGGGTAAACGCGAAAACCGCTACATTCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGa                                                                                        <  2:647614/150‑1 (MQ=255)
                                                             ccGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGaa                                                                                       <  1:1121971/150‑1 (MQ=255)
                                                             ccGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGaa                                                                                       >  1:2961710/1‑150 (MQ=255)
                                                               gcgAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAAc                                                                                     <  1:517482/150‑1 (MQ=255)
                                                                cgAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACa                                                                                    <  2:1202629/150‑1 (MQ=255)
                                                                 gAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAg                                                                                   >  1:2058137/1‑150 (MQ=255)
                                                                   aCCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCg                                                                                 <  1:2297405/150‑1 (MQ=255)
                                                                   aCCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCg                                                                                 <  2:2775589/150‑1 (MQ=255)
                                                                    ccGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGt                                                                                >  2:679253/1‑150 (MQ=255)
                                                                      gggTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACTCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCt                                                                              <  2:2532409/150‑1 (MQ=255)
                                                                         tAAACGCGAAAACCGCTACATGCATCTTTTCATTGGTGAGGTTGAAGATCAGCAGGCGGTGACGGATATGTCGAACTCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGa                                                                           >  1:1729511/1‑150 (MQ=255)
                                                                         tAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGGTGACGGTGATGTACAGGCCCGCGTCGAAGCCCTGGACCTCGAAGTGGCAGAACTGAAACAGCGTCTTGa                                                                           >  2:3220371/1‑150 (MQ=255)
                                                                           aaCGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGAtt                                                                         <  2:3444587/150‑1 (MQ=255)
                                                                           aaCGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGAtt                                                                         >  2:4875138/1‑150 (MQ=255)
                                                                           aaCGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGAtt                                                                         <  2:795440/150‑1 (MQ=255)
                                                                           aaCGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGAtt                                                                         <  1:2917867/150‑1 (MQ=255)
                                                                             cgcgAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCg                                                                       >  1:3449140/1‑150 (MQ=255)
                                                                              gcgAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGt                                                                      <  2:4111279/150‑1 (MQ=255)
                                                                               cgAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGtt                                                                     >  2:1602697/1‑150 (MQ=255)
                                                                                gAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTg                                                                    <  2:400211/150‑1 (MQ=255)
                                                                                 aaaaCCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGc                                                                   <  1:349599/150‑1 (MQ=255)
                                                                                  aaaCCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCt                                                                  >  2:151208/1‑150 (MQ=255)
                                                                                  aaaCCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCt                                                                  <  2:2574589/150‑1 (MQ=255)
                                                                                   aaCCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTg                                                                 <  2:1362835/150‑1 (MQ=255)
                                                                                   aaCCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTg                                                                 >  2:3778625/1‑150 (MQ=255)
                                                                                    aCCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGAGTCGTTGCTgg                                                                >  2:2908370/1‑150 (MQ=255)
                                                                                       gCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGccc                                                             <  2:619292/150‑1 (MQ=255)
                                                                                             tGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTgg                                                       >  1:4209585/1‑150 (MQ=255)
                                                                                                aTCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGgag                                                    <  2:4448131/150‑1 (MQ=255)
                                                                                                aTCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGgag                                                    <  2:5173229/150‑1 (MQ=255)
                                                                                                  cTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACTCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGAt                                                  <  1:3757630/150‑1 (MQ=255)
                                                                                                  cTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGAt                                                  >  2:1165281/1‑150 (MQ=255)
                                                                                                    tttCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTa                                                <  2:4921796/150‑1 (MQ=255)
                                                                                                    tttCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTa                                                <  2:3868074/150‑1 (MQ=255)
                                                                                                    tttCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTa                                                <  2:18186/150‑1 (MQ=255)
                                                                                                     ttCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTaa                                               >  2:4269871/1‑150 (MQ=255)
                                                                                                     ttCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTaa                                               >  2:233783/1‑150 (MQ=255)
                                                                                                        aGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGt                                            >  1:87417/1‑150 (MQ=255)
                                                                                                        aGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGt                                            >  1:5218459/1‑150 (MQ=255)
                                                                                                        aGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGt                                            >  1:160238/1‑150 (MQ=255)
                                                                                                             tgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaaa                                       >  2:2872479/1‑150 (MQ=255)
                                                                                                                ggTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAAt                                    >  1:2353228/1‑150 (MQ=255)
                                                                                                                ggTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAAt                                    <  1:1913258/150‑1 (MQ=255)
                                                                                                                 gTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAAtt                                   <  1:3068912/150‑1 (MQ=255)
                                                                                                                    gAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACg                                <  1:2654291/150‑1 (MQ=255)
                                                                                                                    gAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACg                                <  1:566214/150‑1 (MQ=255)
                                                                                                                    gAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACg                                <  2:1669681/150‑1 (MQ=255)
                                                                                                                     aaGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGt                               >  1:2162263/1‑150 (MQ=255)
                                                                                                                     aaGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCGGGGAGATTAAAGTGAAAAAATTACGt                               >  1:4885294/1‑150 (MQ=255)
                                                                                                                       gATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTAt                             >  2:2448492/1‑150 (MQ=255)
                                                                                                                             ccGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGt                       <  1:2543054/150‑1 (MQ=255)
                                                                                                                              cggcggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTa                      >  1:1256592/1‑150 (MQ=255)
                                                                                                                              cggcggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTa                      >  1:5249909/1‑150 (MQ=255)
                                                                                                                                 cggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTg                   >  1:2476155/1‑150 (MQ=255)
                                                                                                                                     gACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGAt               <  1:1985503/150‑1 (MQ=255)
                                                                                                                                      aCGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGAtt              >  2:5337601/1‑150 (MQ=255)
                                                                                                                                        ggATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAg            <  1:5348780/150‑1 (MQ=255)
                                                                                                                                        ggATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAg            >  1:5402193/1‑150 (MQ=255)
                                                                                                                                            atGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTgg        >  1:2957947/1‑150 (MQ=255)
                                                                                                                                            atGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTgg        >  2:309887/1‑150 (MQ=255)
                                                                                                                                            atGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGAGAAAAAATAACGTATCGGCGTAGTGGGATTAGGTgg        >  1:944824/1‑150 (MQ=255)
                                                                                                                                                  aaCGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTgc  <  1:389648/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGC  >  CP009273/1122010‑1122305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑