BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,129,182	G→A	5.9%	A385T (GCC→ACC)	flgE →	flagellar hook protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,129,182	0	G	A	5.9%	1036.1 / 35.6	290	A385T (GCC→ACC)	flgE	flagellar hook protein
Reads supporting (aligned to +/- strand): ref base G (148/125); new base A (8/9); total (156/134)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 293 total aligned reads displayed.

CGACGCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTAA  >  CP009273/1129033‑1129331

    gCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCa                                                                                                                                                   >  1:4099927/1‑150 (MQ=255)

    gCAATCTTATGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCa                                                                                                                                                   >  2:1939506/1‑150 (MQ=255)

      aaTCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGa                                                                                                                                                 <  1:4059634/150‑1 (MQ=255)

        tcttctGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGAcc                                                                                                                                               <  1:230144/150‑1 (MQ=255)

          ttctGGCGTGGCGCTGTTGGGGTCAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCAt                                                                                                                                             <  2:3990341/150‑1 (MQ=255)

          ttctGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCAt                                                                                                                                             <  1:1186285/150‑1 (MQ=255)

          ttctGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCAt                                                                                                                                             <  2:1257341/150‑1 (MQ=255)

                cgtggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAAc                                                                                                                                       <  1:3340476/150‑1 (MQ=255)

                 gtggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAAcc                                                                                                                                      >  2:28518/1‑150 (MQ=255)

                 gtggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAAcc                                                                                                                                      <  1:3155849/150‑1 (MQ=255)

                    gcgcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAg                                                                                                                                   <  2:5411903/150‑1 (MQ=255)

                    gcgcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAg                                                                                                                                   <  2:3917812/150‑1 (MQ=255)

                    gcgcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAg                                                                                                                                   <  2:3416441/150‑1 (MQ=255)

                      gcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGa                                                                                                                                 >  1:104852/1‑150 (MQ=255)

                      gcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGa                                                                                                                                 >  2:2457464/1‑150 (MQ=255)

                      gcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGa                                                                                                                                 >  1:2491874/1‑150 (MQ=255)

                      gcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGa                                                                                                                                 <  2:4197495/150‑1 (MQ=255)

                      gcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGa                                                                                                                                 >  2:4441611/1‑150 (MQ=255)

                         gTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCa                                                                                                                              >  1:1891459/1‑150 (MQ=255)

                         gTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCa                                                                                                                              <  1:4345739/150‑1 (MQ=255)

                          ttGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAg                                                                                                                             <  2:3651963/150‑1 (MQ=255)

                          ttGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAg                                                                                                                             <  1:2087390/150‑1 (MQ=255)

                              ggACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATcc                                                                                                                         >  2:3297720/1‑150 (MQ=255)

                              ggACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATcc                                                                                                                         <  1:293181/150‑1 (MQ=255)

                              ggACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATcc                                                                                                                         >  2:4928945/1‑150 (MQ=255)

                                aCAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTc                                                                                                                       >  2:2651990/1‑150 (MQ=255)

                                aCAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTc                                                                                                                       >  2:2142228/1‑150 (MQ=255)

                                aCAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTc                                                                                                                       >  2:5360363/1‑150 (MQ=255)

                                 cAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCa                                                                                                                      <  2:3001674/150‑1 (MQ=255)

                                     cgggactggaAACTCTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCTATCTCAGTTAAGAACTGGTCAATATTATCGTTGCCCAGCGTAACTATCAGTCTAACGCACAGACCATCTACACCCAGGACCAGATCCTCAacac                                                                                                                  <  2:2677782/150‑1 (MQ=255)

                                      gggacgggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACg                                                                                                                 >  1:1078742/1‑150 (MQ=255)

                                           gggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGt                                                                                                            <  1:1379406/150‑1 (MQ=255)

                                            ggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGtt                                                                                                           <  2:3453102/150‑1 (MQ=255)

                                            ggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGtt                                                                                                           <  2:1323789/150‑1 (MQ=255)

                                             gaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTa                                                                                                          >  1:1627413/1‑150 (MQ=255)

                                                aCTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACt                                                                                                       >  1:1798315/1‑150 (MQ=255)

                                                aCTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACt                                                                                                       <  1:146615/150‑1 (MQ=255)

                                                aCTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGCCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACt                                                                                                       >  1:4848049/1‑150 (MQ=255)

                                                   ttGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTAc                                                                                                    >  1:3775184/1‑150 (MQ=255)

                                                     ggCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGc                                                                                                  >  2:73024/1‑150 (MQ=255)

                                                        aCCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTaa                                                                                               >  1:3544393/1‑150 (MQ=255)

                                                           cTGACCTACGGTGCGCTGTAAGCGTCCAACGTCGATCTCTGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTACTCg                                                                                            <  2:4992447/150‑1 (MQ=255)

                                                             gACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCt                                                                                          >  2:4339272/1‑150 (MQ=255)

                                                               ccAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGa                                                                                        <  1:372319/150‑1 (MQ=255)

                                                                cAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGAc                                                                                       >  2:3690245/1‑150 (MQ=255)

                                                                cAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGAc                                                                                       >  1:1767173/1‑150 (MQ=255)

                                                                 aaCGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACg                                                                                      <  2:2539232/150‑1 (MQ=255)

                                                                 aaCGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACg                                                                                      <  1:3124195/150‑1 (MQ=255)

                                                                   cGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACggg                                                                                    >  2:2709254/1‑150 (MQ=255)

                                                                          cTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTc                                                                             <  2:1708333/150‑1 (MQ=255)

                                                                           tGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCa                                                                            >  1:5470593/1‑150 (MQ=255)

                                                                             gAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAAt                                                                          <  1:646251/150‑1 (MQ=255)

                                                                             gAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAAt                                                                          <  2:4673207/150‑1 (MQ=255)

                                                                              aaGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCCGCGTAACTATCAGGCTAACGCCCAGACCATCAAAAACCAGGACCAGATCATCAACACGCTGGTTAAATTACGATAATCGCAGACGGGCTAGCACaaag                                                                         >  2:3668133/1‑148 (MQ=255)

                                                                              aaGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATg                                                                         <  1:2264920/150‑1 (MQ=255)

                                                                               aGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGATAACTAACGCTAATCGCTGACGGGATAGCTCAATgg                                                                        >  1:4585225/1‑150 (MQ=255)

                                                                                 cGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGAt                                                                      >  1:3762968/1‑150 (MQ=255)

                                                                                  gTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATc                                                                     <  2:3202685/150‑1 (MQ=255)

                                                                                   tCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCa                                                                    <  1:166293/150‑1 (MQ=255)

                                                                                    ccAACGTCGATCTCAGTAACGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAACACCCAGGACCAGATCCTCAACCCGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCAc                                                                   <  2:4516802/150‑1 (MQ=255)

                                                                                    ccAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCAc                                                                   >  2:612387/1‑150 (MQ=255)

                                                                                      aaCGTCGATCACATTAAATAACTGGACAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCAACAAAACCCAGGACCAGAACCTCAACACGCTGGTTAACTTACTCAAATCGCTGACGGGATAGCTCAAATGATCAccc                                                                 >  1:861161/1‑148 (MQ=255)

                                                                                       aCGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCa                                                                <  2:5125304/150‑1 (MQ=255)

                                                                                          tCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAAtt                                                             <  1:3403998/150‑1 (MQ=255)

                                                                                            gATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTta                                                           >  2:1329038/1‑150 (MQ=255)

                                                                                              tctcAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTtata                                                         >  2:812605/1‑150 (MQ=255)

                                                                                              tctcAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTtata                                                         <  2:1182527/150‑1 (MQ=255)

                                                                                               ctcAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATAc                                                        <  1:4704685/150‑1 (MQ=255)

                                                                                                tcAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATAcc                                                       <  1:4940063/150‑1 (MQ=255)

                                                                                                 cAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACcg                                                      >  1:2681331/1‑150 (MQ=255)

                                                                                                     aaaGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGAt                                                  >  2:532722/1‑150 (MQ=255)

                                                                                                        gAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATggg                                               >  1:4981968/1‑150 (MQ=255)

                                                                                                        gAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATggg                                               <  1:3749623/150‑1 (MQ=255)

                                                                                                          aCTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGag                                             <  2:3855452/150‑1 (MQ=255)

                                                                                                           cTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGagc                                            <  1:4175724/150‑1 (MQ=255)

                                                                                                            tGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGagca                                           >  1:3685395/1‑150 (MQ=255)

                                                                                                             ggTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGagcag                                          <  2:4086053/150‑1 (MQ=255)

                                                                                                              gTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGagcagc                                         >  1:2310493/1‑150 (MQ=255)

                                                                                                              gTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGagcagc                                         <  2:1643723/150‑1 (MQ=255)

                                                                                                                cAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGcagcca                                       <  2:1798315/150‑1 (MQ=255)

                                                                                                                 aaTATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGcagccag                                      >  2:4044643/1‑150 (MQ=255)

                                                                                                                     tGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGccagccag                                  >  1:3879641/1‑150 (MQ=255)

                                                                                                                      gATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGa                                 >  2:2376895/1‑150 (MQ=255)

                                                                                                                         cGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGCTCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGacac                              >  2:4423852/1‑150 (MQ=255)

                                                                                                                         cGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGacac                              <  1:3049540/150‑1 (MQ=255)

                                                                                                                          gTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACt                             >  2:1426843/1‑150 (MQ=255)

                                                                                                                              cccAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAAt                         <  1:343873/150‑1 (MQ=255)

                                                                                                                              cccAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAAt                         >  1:1233160/1‑150 (MQ=255)

                                                                                                                                    gTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAg                   >  1:4895412/1‑150 (MQ=255)

                                                                                                                                    gTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAg                   >  1:5070217/1‑150 (MQ=255)

                                                                                                                                     tAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAgg                  <  1:4055549/150‑1 (MQ=255)

                                                                                                                                          aTCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTa             <  2:3744657/150‑1 (MQ=255)

                                                                                                                                            cAGTCTTACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAAGCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAAc           <  2:2250454/150‑1 (MQ=255)

                                                                                                                                             aGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAAcc          >  1:2439820/1‑150 (MQ=255)

                                                                                                                                                cTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGcc       >  2:66804/1‑150 (MQ=255)

                                                                                                                                                cTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACCGAATCAACAGGCGGTAACCGcc       >  1:5320610/1‑150 (MQ=255)

                                                                                                                                                 tAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCa      >  2:5423484/1‑150 (MQ=255)

                                                                                                                                                  aaCGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAg     >  2:3344811/1‑150 (MQ=255)

                                                                                                                                                   aCGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGt    <  2:1994462/150‑1 (MQ=255)

                                                                                                                                                   aCGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGt    >  1:2820224/1‑150 (MQ=255)

CGACGCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTAA  >  CP009273/1129033‑1129331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`