Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,780,104 C→A 6.4% A422A (GCG→GCT)  ppsA ← phosphoenolpyruvate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,780,1040CA 6.4% 1123.7 / 41.9 294A422A (GCG→GCT) ppsAphosphoenolpyruvate synthase
Reads supporting (aligned to +/- strand):  ref base C (126/149);  new base A (9/10);  total (135/159)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 308 total aligned reads displayed.

TTCACGCTAAATTCCAGCAACTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGG  >  CP009273/1779955‑1780253
                                                                                                                                                     |                                                                                                                                                     
     gCTAAATTCCAGCAACTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTg                                                                                                                                                  <  2:2547345/150‑1 (MQ=255)
      cTAAATTCCAGCAACTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGa                                                                                                                                                 <  1:1962325/150‑1 (MQ=255)
      cTAAATTCCAGCAACTCCGCATAAACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCGACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGa                                                                                                                                                 <  2:1040143/150‑1 (MQ=255)
       tAAATTCCAGCAACTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGAc                                                                                                                                                <  1:3057845/150‑1 (MQ=255)
        aaaTTCCAGCAACTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACa                                                                                                                                               >  1:3426308/1‑150 (MQ=255)
         aaTTCCAGCAACTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAg                                                                                                                                              >  1:1368597/1‑150 (MQ=255)
           ttCCAGCAACTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCAGCGTGACAGGt                                                                                                                                            <  1:5073643/150‑1 (MQ=255)
           ttCCAGCAACTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCAACCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGt                                                                                                                                            >  1:3279768/1‑150 (MQ=255)
             ccAGCAACTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTAc                                                                                                                                          <  1:1731381/150‑1 (MQ=255)
               aGCAACTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGa                                                                                                                                        >  2:3485700/1‑150 (MQ=255)
                  aaCTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCg                                                                                                                                     >  1:149414/1‑150 (MQ=255)
                    cTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGcc                                                                                                                                   <  1:958223/150‑1 (MQ=255)
                       cGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACg                                                                                                                                <  2:3808177/150‑1 (MQ=255)
                          aTAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGtt                                                                                                                             <  2:4058798/150‑1 (MQ=255)
                          aTAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCAGCGTGACAGGTACGACCGCCACGGtt                                                                                                                             <  2:3931778/150‑1 (MQ=255)
                           tAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTg                                                                                                                            <  2:2617412/150‑1 (MQ=255)
                             gACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGt                                                                                                                          >  1:4379402/1‑150 (MQ=255)
                              aCGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTg                                                                                                                         <  2:4352677/150‑1 (MQ=255)
                              aCGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTg                                                                                                                         <  2:4399312/150‑1 (MQ=255)
                               cGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGa                                                                                                                        <  2:3289882/150‑1 (MQ=255)
                                 tAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACg                                                                                                                      >  1:811226/1‑150 (MQ=255)
                                      ggTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGc                                                                                                                 >  1:4130079/1‑150 (MQ=255)
                                        tATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCCCAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAg                                                                                                               <  1:4463783/150‑1 (MQ=255)
                                        tATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCAGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAg                                                                                                               >  2:4793485/1‑150 (MQ=255)
                                         aTCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGa                                                                                                              >  2:4276508/1‑150 (MQ=255)
                                         aTCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGa                                                                                                              <  2:3357207/150‑1 (MQ=255)
                                           cACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATg                                                                                                            >  1:3923052/1‑150 (MQ=255)
                                           cACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCCTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATg                                                                                                            >  2:3738425/1‑150 (MQ=255)
                                               ttCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCCCAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCAGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCttt                                                                                                        <  1:4811/150‑1 (MQ=255)
                                                tCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACCGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTttc                                                                                                       >  1:3208963/1‑150 (MQ=255)
                                                 cGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTttct                                                                                                      >  1:5327017/1‑150 (MQ=255)
                                                 cGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTttct                                                                                                      >  1:1894147/1‑150 (MQ=255)
                                                  ggCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTttctt                                                                                                     <  2:5325625/150‑1 (MQ=255)
                                                  ggCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTttctt                                                                                                     <  2:2308074/150‑1 (MQ=255)
                                                    cacaAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCa                                                                                                   <  2:4498947/150‑1 (MQ=255)
                                                     acaAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCCCAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCAt                                                                                                  <  1:496187/150‑1 (MQ=255)
                                                       aaGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGa                                                                                                >  1:1944052/1‑150 (MQ=255)
                                                        aGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGAt                                                                                               <  2:1077208/150‑1 (MQ=255)
                                                          aaaCAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCg                                                                                             >  2:4237943/1‑150 (MQ=255)
                                                          aaaCAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCg                                                                                             >  2:1715274/1‑150 (MQ=255)
                                                           aaCAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCgg                                                                                            >  2:1892281/1‑150 (MQ=255)
                                                           aaCAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACGACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCgg                                                                                            <  1:2214880/150‑1 (MQ=255)
                                                            aCAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCCCAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGt                                                                                           <  1:5123275/150‑1 (MQ=255)
                                                            aCAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGt                                                                                           <  1:5291029/150‑1 (MQ=255)
                                                              aGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTc                                                                                         <  1:2859934/150‑1 (MQ=255)
                                                               gTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTcc                                                                                        <  2:1909452/150‑1 (MQ=255)
                                                               gTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTcc                                                                                        >  1:3414477/1‑150 (MQ=255)
                                                                   cGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGt                                                                                    >  2:1545794/1‑150 (MQ=255)
                                                                   cGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGt                                                                                    >  1:1819956/1‑150 (MQ=255)
                                                                     ttCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTcc                                                                                  <  2:3134967/150‑1 (MQ=255)
                                                                       ctcACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCgg                                                                                <  2:2436739/150‑1 (MQ=255)
                                                                       ctcACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCgg                                                                                <  2:2436852/150‑1 (MQ=255)
                                                                        tcACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCggg                                                                               <  2:804884/150‑1 (MQ=255)
                                                                        tcACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCggg                                                                               <  1:5383709/150‑1 (MQ=255)
                                                                            cGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGggtcgg                                                                           <  1:1218006/150‑1 (MQ=255)
                                                                            cGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGggtcgg                                                                           >  2:5198177/1‑150 (MQ=255)
                                                                               cTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCa                                                                        >  2:1934921/1‑150 (MQ=255)
                                                                                tttCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCAt                                                                       <  2:4640936/150‑1 (MQ=255)
                                                                                 ttCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATg                                                                      >  1:1592405/1‑150 (MQ=255)
                                                                                   cATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTc                                                                    >  1:716498/1‑150 (MQ=255)
                                                                                    aTCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCa                                                                   >  2:1265584/1‑150 (MQ=255)
                                                                                     tCCGTTCTGTTGCATCTCCCCAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAg                                                                  <  1:2263303/150‑1 (MQ=255)
                                                                                          tCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAAcc                                                             >  2:3076727/1‑150 (MQ=255)
                                                                                           cTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCa                                                            <  2:223616/150‑1 (MQ=255)
                                                                                            tGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAg                                                           <  1:4309494/150‑1 (MQ=255)
                                                                                             gTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGc                                                          >  1:4633040/1‑150 (MQ=255)
                                                                                               tGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCAc                                                        >  2:1291055/1‑150 (MQ=255)
                                                                                               tGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCAc                                                        <  1:140638/150‑1 (MQ=255)
                                                                                                  aTCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTc                                                     >  1:2975757/1‑150 (MQ=255)
                                                                                                   tctcCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCg                                                    <  2:4083216/150‑1 (MQ=255)
                                                                                                        acaGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAgg                                               >  2:2724299/1‑150 (MQ=255)
                                                                                                               aCTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATg                                        <  1:5375837/150‑1 (MQ=255)
                                                                                                                  aCCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCgg                                     <  1:4783634/150‑1 (MQ=255)
                                                                                                                      ccgGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTGAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCa                                 <  2:2323593/150‑1 (MQ=255)
                                                                                                                      ccgGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCa                                 <  1:5391819/150‑1 (MQ=255)
                                                                                                                        ggAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCAtt                               >  2:4939830/1‑150 (MQ=255)
                                                                                                                         gAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCAttt                              >  2:4034460/1‑150 (MQ=255)
                                                                                                                            tGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGc                                                          <  2:2017774/119‑1 (MQ=255)
                                                                                                                            tGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGc                                                          >  1:2017774/1‑119 (MQ=255)
                                                                                                                              cccAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTg                         >  2:3568908/1‑150 (MQ=255)
                                                                                                                                cAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGAt                       <  2:1989834/150‑1 (MQ=255)
                                                                                                                                 aGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATg                      <  1:101215/150‑1 (MQ=255)
                                                                                                                                 aGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATg                      <  1:670678/150‑1 (MQ=255)
                                                                                                                                 aGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATg                      >  2:1008514/1‑150 (MQ=255)
                                                                                                                                  gTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGt                     >  2:547692/1‑150 (MQ=255)
                                                                                                                                   ttCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTc                    <  2:1368597/150‑1 (MQ=255)
                                                                                                                                       cGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATgg                >  1:4694096/1‑150 (MQ=255)
                                                                                                                                        gAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGa               >  2:713210/1‑150 (MQ=255)
                                                                                                                                        gAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGa               >  2:2929252/1‑150 (MQ=255)
                                                                                                                                            gatgatCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGAc           <  1:3485700/150‑1 (MQ=255)
                                                                                                                                             atgatCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACt          >  2:3646067/1‑150 (MQ=255)
                                                                                                                                              tgatCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACtt         <  1:1916823/150‑1 (MQ=255)
                                                                                                                                              tgatCGCAGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACtt         >  1:3818527/1‑150 (MQ=255)
                                                                                                                                               gatCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACttt        <  1:239116/150‑1 (MQ=255)
                                                                                                                                               gatCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACttt        <  1:5206290/150‑1 (MQ=255)
                                                                                                                                                 tCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCa      <  1:4245706/150‑1 (MQ=255)
                                                                                                                                                  cgccgcGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCAc     <  1:2344167/150‑1 (MQ=255)
                                                                                                                                                   gccgcGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCAcc    <  1:52356/150‑1 (MQ=255)
                                                                                                                                                     cgcgTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCgg  <  2:5010120/150‑1 (MQ=255)
                                                                                                                                                     cgcgTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCgg  <  1:4557136/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCACGCTAAATTCCAGCAACTCCGCATAGACGTAACCGGTATCACCTTCGGCACAAGAAACAGTGACGTTCTCACCGTCTTTCATCCGTTCTGTTGCATCTCCACAGCCCACTACCGCCGGAATGCCCAGTTCACGAGCGATGATCGCCGCGTGACAGGTACGACCGCCACGGTTGGTGACGATGGCAGATGCTTTCTTCATGATCGGTTCCCAGTCCGGGTCGGTCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGG  >  CP009273/1779955‑1780253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑