BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,795,380	A→T	62.5%	V483E (GTA→GAA)	thrS ←	threonyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,795,380	0	A	T	62.5%	314.9 / inf	291	V483E (GTA→GAA)	thrS	threonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (39/70); new base T (86/96); total (125/166)
Fisher's exact test for biased strand distribution p-value = 6.65e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 304 total aligned reads displayed.

GAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCCAC  >  CP009273/1795232‑1795529

      aGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTAcc                                                                                                                                                <  2:2414202/150‑1 (MQ=255)

      aGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTAcc                                                                                                                                                <  1:2155551/150‑1 (MQ=255)

       gCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCg                                                                                                                                               >  1:934820/1‑150 (MQ=255)

           aCTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACt                                                                                                                                           >  1:635033/1‑150 (MQ=255)

            ctctTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTg                                                                                                                                          >  2:1379490/1‑150 (MQ=255)

                  ggTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGc                                                                                                                                    >  1:1752854/1‑150 (MQ=255)

                    tCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACCTAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCAc                                                                                                                                  <  2:1734297/150‑1 (MQ=255)

                    tCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCAc                                                                                                                                  <  2:4459530/150‑1 (MQ=255)

                     cAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCAGGCAct                                                                                                                                 >  2:2811209/1‑149 (MQ=255)

                     cAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACg                                                                                                                                 <  2:463468/150‑1 (MQ=255)

                        gATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATc                                                                                                                              <  1:1590343/150‑1 (MQ=255)

                            ccGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAgg                                                                                                                          >  2:2425388/1‑150 (MQ=255)

                            ccGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAgg                                                                                                                          <  2:4112683/150‑1 (MQ=255)

                             cGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGc                                                                                                                         <  1:439619/150‑1 (MQ=255)

                                 gAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTc                                                                                                                     >  1:956675/1‑150 (MQ=255)

                                 gAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTc                                                                                                                     <  2:800433/150‑1 (MQ=255)

                                   aaCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCAt                                                                                                                   <  1:3730879/150‑1 (MQ=255)

                                   aaCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCAt                                                                                                                   <  1:1135250/150‑1 (MQ=255)

                                    aCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATa                                                                                                                  <  1:2045845/150‑1 (MQ=255)

                                       ttCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAg                                                                                                               <  2:4266517/150‑1 (MQ=255)

                                       ttCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAg                                                                                                               <  2:1538279/150‑1 (MQ=255)

                                       ttCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAg                                                                                                               <  2:2755328/150‑1 (MQ=255)

                                        tCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCATGATCGAGGCAGTCATACAgg                                                                                                              >  2:2175051/1‑150 (MQ=255)

                                        tCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAgg                                                                                                              <  1:3082182/150‑1 (MQ=255)

                                        tCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAgg                                                                                                              <  1:517979/150‑1 (MQ=255)

                                           aTCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTa                                                                                                           <  1:1354347/150‑1 (MQ=255)

                                             cGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTaaa                                                                                                         >  1:479270/1‑150 (MQ=255)

                                             cGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTaaa                                                                                                         >  1:716382/1‑150 (MQ=255)

                                              gACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAAt                                                                                                        <  1:3296451/150‑1 (MQ=255)

                                               aCCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAAtt                                                                                                       <  2:3841600/150‑1 (MQ=255)

                                                    aGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAAtt                                                                                                  >  1:3399869/1‑150 (MQ=255)

                                                    aGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAAtt                                                                                                  <  1:4344334/150‑1 (MQ=255)

                                                      aaTTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAAtttt                                                                                                >  2:1273756/1‑150 (MQ=255)

                                                        ttGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCg                                                                                              <  1:2792448/150‑1 (MQ=255)

                                                         tGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCgg                                                                                             >  1:1202388/1‑150 (MQ=255)

                                                           cgcgGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGAc                                                                                           <  1:4026930/150‑1 (MQ=255)

                                                           cgcgGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGAc                                                                                           <  1:30624/150‑1 (MQ=255)

                                                             cgGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCg                                                                                         <  1:4048050/150‑1 (MQ=255)

                                                               gTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTa                                                                                       <  2:2701656/150‑1 (MQ=255)

                                                                 gAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATACGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGa                                                                                     >  2:2914164/1‑150 (MQ=255)

                                                                  aaTCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGaa                                                                                    >  1:4401270/1‑150 (MQ=255)

                                                                    tCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAg                                                                                  <  1:5007382/150‑1 (MQ=255)

                                                                     cATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAgc                                                                                 <  2:2005221/150‑1 (MQ=255)

                                                                     cATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAgc                                                                                 <  1:3945521/150‑1 (MQ=255)

                                                                      aTTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAgcg                                                                                >  1:5386731/1‑150 (MQ=255)

                                                                        tACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGcc                                                                              >  1:2932409/1‑150 (MQ=255)

                                                                         aCCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCt                                                                             <  1:3145591/150‑1 (MQ=255)

                                                                           cGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTc                                                                           <  1:4197979/150‑1 (MQ=255)

                                                                            ggTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCa                                                                          >  1:1096103/1‑150 (MQ=255)

                                                                               aCTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCAccc                                                                       >  2:3426497/1‑150 (MQ=255)

                                                                                 tttACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAg                                                                     >  1:1541703/1‑150 (MQ=255)

                                                                                 tttACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAg                                                                     >  2:2077737/1‑150 (MQ=255)

                                                                                 tttACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAg                                                                     >  2:1501866/1‑150 (MQ=255)

                                                                                    aCGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTg                                                                  <  1:3592782/150‑1 (MQ=255)

                                                                                    aCGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTg                                                                  <  1:4942711/150‑1 (MQ=255)

                                                                                    aCGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTg                                                                  <  1:4150273/150‑1 (MQ=255)

                                                                                      gTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGat                                                                >  2:5269924/1‑150 (MQ=255)

                                                                                      gTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGat                                                                >  1:2546225/1‑150 (MQ=255)

                                                                                      gTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGat                                                                >  2:1895981/1‑150 (MQ=255)

                                                                                       ttCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGata                                                               <  2:1820884/150‑1 (MQ=255)

                                                                                       ttCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGata                                                               >  1:1481601/1‑150 (MQ=255)

                                                                                       ttCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGata                                                               >  1:3976607/1‑150 (MQ=255)

                                                                                          aTTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTc                                                            >  2:3247971/1‑150 (MQ=255)

                                                                                              tCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAAc                                                        >  1:4446456/1‑150 (MQ=255)

                                                                                               cTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACg                                                       <  2:72645/150‑1 (MQ=255)

                                                                                               cTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACg                                                       <  2:5036824/150‑1 (MQ=255)

                                                                                                ttCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACgg                                                      >  2:4903403/1‑150 (MQ=255)

                                                                                                    ccTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtg                                                  <  2:226131/150‑1 (MQ=255)

                                                                                                    ccTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtg                                                  >  2:2622127/1‑150 (MQ=255)

                                                                                                      tACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtgtt                                                >  1:2231527/1‑150 (MQ=255)

                                                                                                         aTAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATgttgtt                                             >  1:4146461/1‑150 (MQ=255)

                                                                                                         aTAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATgttgtt                                             >  2:1121508/1‑150 (MQ=255)

                                                                                                          tAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGttt                                            <  2:5291544/150‑1 (MQ=255)

                                                                                                             gaagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTttct                                         <  2:5459974/150‑1 (MQ=255)

                                                                                                             gaagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTttct                                         <  1:2573347/150‑1 (MQ=255)

                                                                                                              aagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTttctt                                        >  2:5391156/1‑150 (MQ=255)

                                                                                                                gcgcTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTcc                                      <  1:1804716/150‑1 (MQ=255)

                                                                                                                gcgcTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTcc                                      <  1:2840583/150‑1 (MQ=255)

                                                                                                                gcgcTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTcc                                      <  1:2568841/150‑1 (MQ=255)

                                                                                                                 cgcTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCa                                     <  2:2209320/150‑1 (MQ=255)

                                                                                                                    tCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAgcg                                  <  2:4575344/150‑1 (MQ=255)

                                                                                                                     cAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAgcgc                                 >  1:2964566/1‑150 (MQ=255)

                                                                                                                        acgagacgGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAAc                              <  1:3906719/150‑1 (MQ=255)

                                                                                                                          gagacgGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCg                            >  1:1025173/1‑150 (MQ=255)

                                                                                                                            gacgGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGcc                          <  2:4078654/150‑1 (MQ=255)

                                                                                                                            gacgGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGcc                          <  1:2303025/150‑1 (MQ=255)

                                                                                                                              cgGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAg                        <  2:3109729/150‑1 (MQ=255)

                                                                                                                               ggCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAgg                       >  2:4348734/1‑150 (MQ=255)

                                                                                                                                gCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGt                      >  2:4619876/1‑150 (MQ=255)

                                                                                                                                     gagaAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGcc                 >  2:604416/1‑150 (MQ=255)

                                                                                                                                       gaAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTc               <  2:698313/150‑1 (MQ=255)

                                                                                                                                       gaAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTc               <  1:4648238/150‑1 (MQ=255)

                                                                                                                                       gaAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTc               >  2:4304233/1‑150 (MQ=255)

                                                                                                                                          gTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGc            <  2:4803061/150‑1 (MQ=255)

                                                                                                                                            ccAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCAc          <  2:1691384/150‑1 (MQ=255)

                                                                                                                                               gCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGAt       <  1:1391160/150‑1 (MQ=255)

                                                                                                                                                ctgtactgtaCCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATc      <  2:3753962/150‑1 (MQ=255)

                                                                                                                                                ctgtactgtaCCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATc      <  2:113823/150‑1 (MQ=255)

                                                                                                                                                  gtactgtacCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATccc    <  2:265326/150‑1 (MQ=255)

                                                                                                                                                  gtactgtacCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATccc    <  1:236554/150‑1 (MQ=255)

GAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCCAC  >  CP009273/1795232‑1795529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`