BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,403,195	G→T	6.5%	S546* (TCG→TAG)	yfbS ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,403,195	0	G	T	6.5%	1281.1 / 49.0	335	S546* (TCG→TAG)	yfbS	putative transporter
Reads supporting (aligned to +/- strand): ref base G (178/135); new base T (14/8); total (192/143)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 343 total aligned reads displayed.

CGATAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATC  >  CP009273/2403046‑2403344

 gATAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGa                                                                                                                                                      <  1:1391951/150‑1 (MQ=255)

  aTAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGac                                                                                                                                                     >  1:3680194/1‑150 (MQ=255)

  aTAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGac                                                                                                                                                     <  1:3085767/150‑1 (MQ=255)

   tAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGaca                                                                                                                                                    <  1:2888616/150‑1 (MQ=255)

   tAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGaca                                                                                                                                                    <  1:836474/150‑1 (MQ=255)

    aaTGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGacac                                                                                                                                                   >  2:810756/1‑150 (MQ=255)

          gAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCAt                                                                                                                                             >  1:1549032/1‑150 (MQ=255)

          gAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCAt                                                                                                                                             >  1:5473637/1‑150 (MQ=255)

          gAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCACGGACCTAAAACCAGTGTGTTAACAGGTGAAGAACCCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCACCAt                                                                                                                                             >  1:3066573/1‑150 (MQ=255)

              ggTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGtt                                                                                                                                         <  1:1009412/150‑1 (MQ=255)

               gTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCTACACCCCCATCGttt                                                                                                                                        >  2:5343005/1‑150 (MQ=255)

                tACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGtttt                                                                                                                                       <  1:4253074/150‑1 (MQ=255)

                  cccccAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCTACACCCCCATCGTTTTgg                                                                                                                                     >  1:533037/1‑150 (MQ=255)

                    cccAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGgca                                                                                                                                   >  2:1346550/1‑150 (MQ=255)

                    cccAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGgca                                                                                                                                   >  1:4325572/1‑150 (MQ=255)

                    cccAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGgca                                                                                                                                   >  2:46471/1‑150 (MQ=255)

                       aaCTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGgcagca                                                                                                                                <  2:325853/150‑1 (MQ=255)

                            cacaAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGcagccag                                                                                                                           <  1:2149789/150‑1 (MQ=255)

                            cacaAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGcagccag                                                                                                                           <  1:2338391/150‑1 (MQ=255)

                              caAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAgcg                                                                                                                         >  1:3273854/1‑150 (MQ=255)

                                aaGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCCTCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCa                                                                                                                       >  2:1855807/1‑150 (MQ=255)

                                  gTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAAt                                                                                                                     <  2:5455801/150‑1 (MQ=255)

                                   tCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATc                                                                                                                    <  1:2212299/150‑1 (MQ=255)

                                   tCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATc                                                                                                                    <  2:3032768/150‑1 (MQ=255)

                                        gAAGCTGTAATTTCCCGGACCTAAACCCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGTTGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCTACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGc                                                                                                               <  1:1581600/150‑1 (MQ=255)

                                        gAAGCTGTAATGTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGc                                                                                                               <  1:5020243/150‑1 (MQ=255)

                                             tGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCa                                                                                                          >  2:2403203/1‑150 (MQ=255)

                                               tAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCCTCAac                                                                                                        >  2:4433685/1‑150 (MQ=255)

                                                 aTTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAacac                                                                                                      <  1:1823094/150‑1 (MQ=255)

                                                   ttCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACAccg                                                                                                    >  1:3973465/1‑150 (MQ=255)

                                                     cccGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACAccgcc                                                                                                  >  1:994476/1‑150 (MQ=255)

                                                        ggACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTc                                                                                               >  1:3785250/1‑150 (MQ=255)

                                                         gACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTcg                                                                                              <  1:5035758/150‑1 (MQ=255)

                                                           ccTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTcgcg                                                                                            <  1:2465260/150‑1 (MQ=255)

                                                               aaaCCCGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAAGGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTAt                                                                                        <  1:3475474/150‑1 (MQ=255)

                                                               aaaCCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTAt                                                                                        <  2:3312400/150‑1 (MQ=255)

                                                                aaCCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTAtt                                                                                       >  1:1641870/1‑150 (MQ=255)

                                                                 aCCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGACTGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTa                                                                                      <  2:795559/150‑1 (MQ=255)

                                                                  ccAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTag                                                                                     >  2:378160/1‑150 (MQ=255)

                                                                   cAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCTACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTaga                                                                                    >  1:831926/1‑150 (MQ=255)

                                                                   cAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTaga                                                                                    >  2:1804890/1‑150 (MQ=255)

                                                                    aGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTagag                                                                                   >  2:2367727/1‑150 (MQ=255)

                                                                    aGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTagag                                                                                   <  2:529060/150‑1 (MQ=255)

                                                                     gtgtgtTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAgaga                                                                                  <  2:4993410/150‑1 (MQ=255)

                                                                      tgtgtTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGAt                                                                                 >  2:2089404/1‑150 (MQ=255)

                                                                       gtgtTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATa                                                                                <  2:221929/150‑1 (MQ=255)

                                                                       gtgtTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATa                                                                                <  1:2044954/150‑1 (MQ=255)

                                                                       gtgtTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATa                                                                                >  1:1857005/1‑150 (MQ=255)

                                                                         gtTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATaaa                                                                              >  2:2245228/1‑150 (MQ=255)

                                                                          ttAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAAt                                                                             <  2:1072731/150‑1 (MQ=255)

                                                                            aaCAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAg                                                                           <  1:3368661/150‑1 (MQ=255)

                                                                            aaCAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAg                                                                           >  1:887848/1‑150 (MQ=255)

                                                                             aCAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGc                                                                          <  2:49066/150‑1 (MQ=255)

                                                                              cAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGcc                                                                         >  2:859861/1‑150 (MQ=255)

                                                                               aGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGccc                                                                        >  2:2086308/1‑150 (MQ=255)

                                                                                ggTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCa                                                                       >  2:1237538/1‑150 (MQ=255)

                                                                                ggTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCa                                                                       <  2:1657331/150‑1 (MQ=255)

                                                                                    aagaaACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATaa                                                                   >  1:67473/1‑150 (MQ=255)

                                                                                    aagaaACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATaa                                                                   <  2:2627007/150‑1 (MQ=255)

                                                                                      naaaCCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCCTCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATCAATAGCCCAATAAcc                                                                 >  2:415731/2‑150 (MQ=255)

                                                                                       aaaCCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAAccg                                                                <  1:506254/150‑1 (MQ=255)

                                                                                         aCCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCTACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATCAccgcc                                                              <  2:278560/150‑1 (MQ=255)

                                                                                          ccGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAAccgccg                                                             <  2:47346/150‑1 (MQ=255)

                                                                                          ccGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAAccgccg                                                             <  1:3393562/150‑1 (MQ=255)

                                                                                            ggTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCgac                                                           >  2:649532/1‑150 (MQ=255)

                                                                                             gtgtCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCgaca                                                          >  1:458899/1‑150 (MQ=255)

                                                                                                tCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATAAGAGATAAATAGCCCAATAACCGCCgacaaga                                                       >  2:3058477/1‑150 (MQ=255)

                                                                                                 cATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCgacaagac                                                      <  1:2961664/150‑1 (MQ=255)

                                                                                                  aTAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCgacaagaca                                                     <  1:1059559/150‑1 (MQ=255)

                                                                                                   tAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCgacaagacaa                                                    >  2:4088818/1‑150 (MQ=255)

                                                                                                     aaGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGacaaac                                                  >  1:3698005/1‑150 (MQ=255)

                                                                                                      aGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGacaaaca                                                 >  1:1519905/1‑150 (MQ=255)

                                                                                                             ggATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACAccc                                          >  1:4463849/1‑150 (MQ=255)

                                                                                                             ggATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACAccc                                          <  1:1176113/150‑1 (MQ=255)

                                                                                                              gATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACAcccc                                         >  1:5183798/1‑150 (MQ=255)

                                                                                                              gATGCGGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACAcccc                                         <  2:4289653/150‑1 (MQ=255)

                                                                                                               aTGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCa                                        <  2:679219/150‑1 (MQ=255)

                                                                                                                   tgcCATCGCCTCGACCTTCGCGAATGTATATGGCGACACGCCCATCGTTTTGGCAGCAGCCAGCGCATTCGTAGACATCTTCAACGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAATCAAACACCCCAGcat                                    <  2:4833781/150‑1 (MQ=255)

                                                                                                                     ccATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGcatca                                  <  1:4858244/150‑1 (MQ=255)

                                                                                                                      cATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGcatcat                                 <  2:3680194/150‑1 (MQ=255)

                                                                                                                      cATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGcatcat                                 >  1:3376059/1‑150 (MQ=255)

                                                                                                                       aTCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCata                                >  1:1258856/1‑150 (MQ=255)

                                                                                                                          gCCACGACCATCGCGAATGGATAAGGCGTCACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATAtgt                             <  1:664107/150‑1 (MQ=255)

                                                                                                                           ccACGACCATCGCGAATGGATTAGGCGACACCCCCATCGTTTTGGGAGCAGACAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATACATAGCCCAATAACCGCCGACAAGACAATCAAACACCCCAGCATCATAtgtg                            <  1:2972171/150‑1 (MQ=255)

                                                                                                                           ccACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATAtgtg                            <  1:2552102/150‑1 (MQ=255)

                                                                                                                            cACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTgg                           >  1:165774/1‑150 (MQ=255)

                                                                                                                                aCCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCg                       >  1:4086835/1‑150 (MQ=255)

                                                                                                                                 ccATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCACTCGGAGCCATCAACACCGCCGACGCGGTATTAGAGATAAATAGCCCAAGAACCGCCGACAAGACAAACAACCACCCCAGCAACATATGTGGCACGt                      >  2:2950027/1‑150 (MQ=255)

                                                                                                                                  cATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTa                     >  2:505573/1‑150 (MQ=255)

                                                                                                                                       cgAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAaccac                <  2:1413068/150‑1 (MQ=255)

                                                                                                                                        gAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAaccacc               >  1:2398806/1‑150 (MQ=255)

                                                                                                                                        gAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAaccacc               >  2:3155467/1‑150 (MQ=255)

                                                                                                                                        gAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAaccacc               >  2:2455460/1‑150 (MQ=255)

                                                                                                                                        gAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCAGATGTGGCCCGTAaccacc               >  2:5243681/1‑150 (MQ=255)

                                                                                                                                         aagggATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCTATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCg              <  1:2902024/147‑1 (MQ=255)

                                                                                                                                              aTAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAAt         <  2:3553323/150‑1 (MQ=255)

                                                                                                                                               tAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATg        >  2:1572903/1‑150 (MQ=255)

                                                                                                                                                aaGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATACATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGt       >  2:4562084/1‑150 (MQ=255)

                                                                                                                                                  ggCTACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTcc     <  1:5343005/150‑1 (MQ=255)

                                                                                                                                                     gACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATc  <  2:3911054/150‑1 (MQ=255)

CGATAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATC  >  CP009273/2403046‑2403344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`