BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,982,873	G→T	6.4%	G71* (GGA→TGA)	yqeJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,982,873	0	G	T	6.4%	1269.1 / 44.0	337	G71* (GGA→TGA)	yqeJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (144/169); new base T (11/13); total (155/182)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 341 total aligned reads displayed.

TCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCA  >  CP009273/2982724‑2983022

tCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTATTAATGACCAGGGACGAGCAAATCTTACCATCGAATTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTg                                                                                                                                                       >  2:4910251/1‑150 (MQ=255)

            cTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGcaac                                                                                                                                           >  1:406820/1‑150 (MQ=255)

              tACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGcaacaa                                                                                                                                         >  1:3566205/1‑150 (MQ=255)

                cacTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAgg                                                                                                                                       >  2:634332/1‑150 (MQ=255)

                 acTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGt                                                                                                                                      >  1:134386/1‑150 (MQ=255)

                  cTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTa                                                                                                                                     >  2:3646783/1‑150 (MQ=255)

                    gCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAAc                                                                                                                                   <  2:2020404/150‑1 (MQ=255)

                      aGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACag                                                                                                                                 >  2:1362217/1‑150 (MQ=255)

                      aGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACag                                                                                                                                 >  2:3570842/1‑150 (MQ=255)

                          aaaaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAAACTGGCAACAAGGTAACAGAGaa                                                                                                                             >  1:1016190/1‑150 (MQ=255)

                          aaaaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGaa                                                                                                                             >  1:1419669/1‑150 (MQ=255)

                           aaaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGaaa                                                                                                                            <  1:1995087/150‑1 (MQ=255)

                           aaaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGaaa                                                                                                                            >  1:4242156/1‑150 (MQ=255)

                            aaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAg                                                                                                                           >  2:2939419/1‑150 (MQ=255)

                            aaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAg                                                                                                                           <  2:2413197/150‑1 (MQ=255)

                             aTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGt                                                                                                                          >  1:552799/1‑150 (MQ=255)

                              tGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTa                                                                                                                         <  2:4250572/150‑1 (MQ=255)

                               gATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTaa                                                                                                                        >  2:4416868/1‑150 (MQ=255)

                                atatatAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTaaa                                                                                                                       <  1:1507800/150‑1 (MQ=255)

                                atatatAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGATGAACCTGGCAACAAGGTAACAGAGAAAGTaaa                                                                                                                       >  2:5479085/1‑150 (MQ=255)

                                 tatataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTCACAGAGAAAGTAAAt                                                                                                                      >  2:2672364/1‑150 (MQ=255)

                                 tatataACGAAACATGCACTGAAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAAt                                                                                                                      <  2:3952219/150‑1 (MQ=255)

                                  atataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATc                                                                                                                     >  2:3144961/1‑150 (MQ=255)

                                    ataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCaa                                                                                                                   >  1:4295059/1‑150 (MQ=255)

                                    ataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCaa                                                                                                                   <  1:4757946/150‑1 (MQ=255)

                                    ataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCaa                                                                                                                   >  2:1138639/1‑150 (MQ=255)

                                    ataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCaa                                                                                                                   >  1:3476721/1‑150 (MQ=255)

                                        cGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACg                                                                                                               <  1:3110747/150‑1 (MQ=255)

                                          aaaCATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGc                                                                                                             >  1:3743418/1‑150 (MQ=255)

                                             cATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGaa                                                                                                          >  1:2355346/1‑150 (MQ=255)

                                              aTGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGaaa                                                                                                         >  1:866345/1‑150 (MQ=255)

                                                    tGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGa                                                                                                   >  2:1543889/1‑150 (MQ=255)

                                                      cAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAAt                                                                                                 <  2:4076326/150‑1 (MQ=255)

                                                         aTTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATaca                                                                                              <  1:4674337/150‑1 (MQ=255)

                                                         aTTGGGTAATTTTTAATGAACAGGGACTATCAAATATTACCATCGAATTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAATGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATaca                                                                                              <  2:3338204/150‑1 (MQ=255)

                                                          ttGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATacac                                                                                             >  1:162489/1‑150 (MQ=255)

                                                             ggTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATg                                                                                          >  1:121492/1‑150 (MQ=255)

                                                                aaTTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGAt                                                                                       >  1:1810659/1‑150 (MQ=255)

                                                                aaTTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGAt                                                                                       >  2:3234120/1‑150 (MQ=255)

                                                                      tAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGaaaa                                                                                 >  1:3907442/1‑150 (MQ=255)

                                                                       aaTGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAAc                                                                                >  2:5388727/1‑150 (MQ=255)

                                                                       aaTGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAAc                                                                                <  1:4428534/150‑1 (MQ=255)

                                                                        aTGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACt                                                                               <  2:2345441/150‑1 (MQ=255)

                                                                        aTGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACt                                                                               <  1:4966623/150‑1 (MQ=255)

                                                                         tGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTa                                                                              <  1:1924955/150‑1 (MQ=255)

                                                                         tGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTa                                                                              >  2:3681512/1‑150 (MQ=255)

                                                                            ccAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGa                                                                           <  2:2804536/150‑1 (MQ=255)

                                                                             cAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGac                                                                          <  1:5134717/150‑1 (MQ=255)

                                                                             cAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGac                                                                          <  2:1213735/150‑1 (MQ=255)

                                                                               gggAAGAGCAAATCTTAGCATCGACTTTATGTATAACAAAAAAAATAAAACAGGATCTGTTGCGTTGAGTTGAACCTTGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGacac                                                                        <  2:5212975/150‑1 (MQ=255)

                                                                                ggACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGAcaca                                                                       <  2:3483597/150‑1 (MQ=255)

                                                                                  aCGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGc                                                                     >  1:963/1‑150 (MQ=255)

                                                                                     aGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCa                                                                  >  1:4183393/1‑150 (MQ=255)

                                                                                      gCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAATTTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGATAACTATGACACAGCCCAt                                                                 <  1:2964181/150‑1 (MQ=255)

                                                                                         aaTCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTa                                                              <  2:2400723/150‑1 (MQ=255)

                                                                                         aaTCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTa                                                              <  2:5289984/150‑1 (MQ=255)

                                                                                             ttACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACAt                                                          <  1:2952720/150‑1 (MQ=255)

                                                                                             ttACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACAt                                                          >  2:2299381/1‑150 (MQ=255)

                                                                                              tACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATc                                                         <  2:1846773/150‑1 (MQ=255)

                                                                                                 cATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaa                                                      >  1:2057600/1‑150 (MQ=255)

                                                                                                  aTCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaag                                                     <  2:4901226/150‑1 (MQ=255)

                                                                                                  aTCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaag                                                     <  1:1176927/150‑1 (MQ=255)

                                                                                                  aTCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaag                                                     >  2:1916772/1‑150 (MQ=255)

                                                                                                     gACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaagaaa                                                  <  1:2048469/150‑1 (MQ=255)

                                                                                                     gACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaagaaa                                                  <  1:17297/150‑1 (MQ=255)

                                                                                                     gACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaagaaa                                                  <  1:591901/150‑1 (MQ=255)

                                                                                                     gACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaagaaa                                                  <  1:83964/150‑1 (MQ=255)

                                                                                                       cTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGt                                                >  1:2901124/1‑150 (MQ=255)

                                                                                                       cTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGt                                                >  2:2659533/1‑150 (MQ=255)

                                                                                                       cTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGt                                                <  1:1477312/150‑1 (MQ=255)

                                                                                                         ttATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTta                                              >  1:2550406/1‑150 (MQ=255)

                                                                                                           aTGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTtaat                                            <  2:5118142/150‑1 (MQ=255)

                                                                                                           aTGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTtaat                                            >  1:4257425/1‑150 (MQ=255)

                                                                                                             gTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTtaataa                                          <  1:3218758/150‑1 (MQ=255)

                                                                                                              tataACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAg                                         >  1:1591791/1‑150 (MQ=255)

                                                                                                              tataACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAg                                         <  1:4639729/150‑1 (MQ=255)

                                                                                                              tataACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAg                                         <  1:3393186/150‑1 (MQ=255)

                                                                                                               ataACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGt                                        <  1:3789521/150‑1 (MQ=255)

                                                                                                                      aaaaaaTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGAt                                 <  2:5311683/150‑1 (MQ=255)

                                                                                                                      aaaaaaTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGAt                                 <  2:965186/150‑1 (MQ=255)

                                                                                                                        aaaaTAAAACAGGAACTGTAGAGTTGAGAGGAACCTGGAAACAAGGTAACAGAGAAAGTAAATCAAAACGGCTAAATATTGAATACACATGGATTGAAAACTATGACACAGACCATTTAACCTCAAAGAAAGTTAATAACTTTGAGATTa                               >  2:2432811/1‑150 (MQ=255)

                                                                                                                           aTAAAACAGGAACTTTTGAGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATgg                            <  2:3834422/150‑1 (MQ=255)

                                                                                                                           aTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATgg                            >  2:3891777/1‑150 (MQ=255)

                                                                                                                            tAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGa                           >  1:316963/1‑150 (MQ=255)

                                                                                                                             aaaaCAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGAt                          <  2:791607/150‑1 (MQ=255)

                                                                                                                             aaaaCAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGAt                          <  1:1355660/150‑1 (MQ=255)

                                                                                                                             aaaaCAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGAt                          <  1:3938047/150‑1 (MQ=255)

                                                                                                                                 cAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAg                      <  1:2673945/150‑1 (MQ=255)

                                                                                                                                   ggAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGtt                    >  2:2201516/1‑150 (MQ=255)

                                                                                                                                   ggAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGtt                    <  2:2702348/150‑1 (MQ=255)

                                                                                                                                   ggAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGtt                    <  1:5388727/150‑1 (MQ=255)

                                                                                                                                   ggAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGtt                    >  2:4521272/1‑150 (MQ=255)

                                                                                                                                     aaCTGTTGCGTTGAGTTGTACCTGGCAACAAGGTCACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACAGCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtga                  >  2:4594403/1‑150 (MQ=255)

                                                                                                                                        tGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatga               >  2:3465145/1‑150 (MQ=255)

                                                                                                                                        tGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatga               <  2:3743418/150‑1 (MQ=255)

                                                                                                                                          ttGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatgatg             >  1:1793450/1‑150 (MQ=255)

                                                                                                                                          ttGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatgatg             <  1:2195288/150‑1 (MQ=255)

                                                                                                                                          ttGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatgatg             >  2:473084/1‑150 (MQ=255)

                                                                                                                                           tGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatgatga            <  2:1681803/150‑1 (MQ=255)

                                                                                                                                                     ggAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGca  <  1:5195330/150‑1 (MQ=255)

TCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCA  >  CP009273/2982724‑2983022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`