BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,020,228	G→T	9.6%	A368A (GCG→GCT)	guaD →	guanine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,020,228	0	G	T	9.6%	1155.9 / 79.3	317	A368A (GCG→GCT)	guaD	guanine deaminase
Reads supporting (aligned to +/- strand): ref base G (143/143); new base T (18/13); total (161/156)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 318 total aligned reads displayed.

AAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGAC  >  CP009273/3020080‑3020377

aaGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGa                                                                                                                                                      <  2:3803037/150‑1 (MQ=255)

 aGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGaa                                                                                                                                                     >  1:1397218/1‑150 (MQ=255)

 aGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGaa                                                                                                                                                     >  1:2607036/1‑150 (MQ=255)

  gTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGaaa                                                                                                                                                    >  1:5160494/1‑150 (MQ=255)

   ttAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAAt                                                                                                                                                   >  2:4072726/1‑150 (MQ=255)

    tAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAAtc                                                                                                                                                  >  1:3927223/1‑150 (MQ=255)

     aaaGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCAGATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAAtct                                                                                                                                                 >  1:4797802/1‑150 (MQ=255)

            gCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGcc                                                                                                                                          >  1:3752874/1‑150 (MQ=255)

             cATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCt                                                                                                                                         <  1:2760094/150‑1 (MQ=255)

             cATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCt                                                                                                                                         <  2:4892493/150‑1 (MQ=255)

              aTGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCtt                                                                                                                                        <  2:2412596/150‑1 (MQ=255)

              aTGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCtt                                                                                                                                        >  1:2638849/1‑150 (MQ=255)

                gggAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGa                                                                                                                                      <  2:1531662/150‑1 (MQ=255)

                 ggAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGAc                                                                                                                                     >  1:348018/1‑150 (MQ=255)

                 ggAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGAc                                                                                                                                     <  2:3256735/150‑1 (MQ=255)

                  gAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACg                                                                                                                                    >  2:5451217/1‑150 (MQ=255)

                    aCGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGAt                                                                                                                                  >  1:3127732/1‑150 (MQ=255)

                    aCGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGAt                                                                                                                                  >  2:5069615/1‑150 (MQ=255)

                     cGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGAtt                                                                                                                                 >  2:183123/1‑150 (MQ=255)

                        atatCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGACGATTTGa                                                                                                                              >  1:2247267/1‑150 (MQ=255)

                          atCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGACGATTTGAtt                                                                                                                            <  1:4108477/150‑1 (MQ=255)

                               tGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCaa                                                                                                                       <  1:2848158/150‑1 (MQ=255)

                               tGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCaa                                                                                                                       <  1:3246936/150‑1 (MQ=255)

                                  cGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACtt                                                                                                                    <  1:4842734/150‑1 (MQ=255)

                                   ggAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACttt                                                                                                                   >  2:4722971/1‑150 (MQ=255)

                                     aaCCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACttttt                                                                                                                 >  2:1705728/1‑150 (MQ=255)

                                     aaCCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACttttt                                                                                                                 >  1:1672071/1‑150 (MQ=255)

                                      aCCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTa                                                                                                                <  1:2133252/150‑1 (MQ=255)

                                      aCCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTa                                                                                                                <  2:36925/150‑1 (MQ=255)

                                      aCCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTa                                                                                                                <  2:1332778/150‑1 (MQ=255)

                                        cACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTAcc                                                                                                              <  2:68933/150‑1 (MQ=255)

                                        cACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTAcc                                                                                                              >  2:1407300/1‑150 (MQ=255)

                                         aCTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCt                                                                                                             <  1:2814213/150‑1 (MQ=255)

                                            ttCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGc                                                                                                          >  1:1981348/1‑150 (MQ=255)

                                             tCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCa                                                                                                         <  1:888001/150‑1 (MQ=255)

                                             agaaCATTCTTCTAAAGCTGAACGAAGCCTACAAAGAATATCAATTACAAGGCTATCGCCTCTCGTCATATGAAGCGATTTAACTGGCCACGCTCGTCGGAGAGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCa                                                                                                         <  2:2950615/148‑1 (MQ=255)

                                              cAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCaa                                                                                                        >  2:1178106/1‑150 (MQ=255)

                                                 cATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAaga                                                                                                     >  2:1510506/1‑150 (MQ=255)

                                                     ctgcAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCt                                                                                                 >  1:3682207/1‑150 (MQ=255)

                                                         aaaCGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGAtt                                                                                             >  2:2000777/1‑150 (MQ=255)

                                                         aaaCGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGAtt                                                                                             >  2:4724468/1‑150 (MQ=255)

                                                          aaCGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGAttt                                                                                            <  1:4988677/150‑1 (MQ=255)

                                                            cGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCg                                                                                          <  1:914767/150‑1 (MQ=255)

                                                             gCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCgt                                                                                         >  1:5047040/1‑150 (MQ=255)

                                                              cTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCgtg                                                                                        <  2:2729020/150‑1 (MQ=255)

                                                                   cGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGAt                                                                                   <  1:2153194/150‑1 (MQ=255)

                                                                    gAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATg                                                                                  <  2:4385594/150‑1 (MQ=255)

                                                                          tACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAAccc                                                                            >  2:2486276/1‑150 (MQ=255)

                                                                            cAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCAc                                                                          <  1:2435600/150‑1 (MQ=255)

                                                                                gTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGcc                                                                      >  2:1259459/1‑150 (MQ=255)

                                                                                gTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGcc                                                                      <  1:4036264/150‑1 (MQ=255)

                                                                                 tATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCa                                                                     >  2:74547/1‑150 (MQ=255)

                                                                                   tagCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACt                                                                   <  2:5259440/148‑1 (MQ=255)

                                                                                     gCAATGACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTcc                                                                 <  2:2710858/150‑1 (MQ=255)

                                                                                      cAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGACGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCg                                                                >  1:1144969/1‑150 (MQ=255)

                                                                                        aTTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCt                                                              >  1:841039/1‑150 (MQ=255)

                                                                                            cAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTAcag                                                          <  1:1592735/150‑1 (MQ=255)

                                                                                            cAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTAcag                                                          >  1:4953771/1‑150 (MQ=255)

                                                                                              aGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTAcagca                                                        >  1:3403156/1‑150 (MQ=255)

                                                                                                gCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACagcagc                                                      <  2:3083073/150‑1 (MQ=255)

                                                                                                   aTCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTgc                                                   <  1:5451217/150‑1 (MQ=255)

                                                                                                    tCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTgcg                                                  <  2:815306/150‑1 (MQ=255)

                                                                                                      gCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCt                                                <  2:7094/150‑1 (MQ=255)

                                                                                                       ccTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTa                                               >  2:36455/1‑150 (MQ=255)

                                                                                                        ctctCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTAt                                              <  1:942615/150‑1 (MQ=255)

                                                                                                         tctcGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATg                                             <  2:3752874/150‑1 (MQ=255)

                                                                                                          ctcGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGa                                            <  2:4274977/150‑1 (MQ=255)

                                                                                                               cATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAAct                                       <  2:4236767/150‑1 (MQ=255)

                                                                                                               cATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAAct                                       >  2:32686/1‑150 (MQ=255)

                                                                                                                atatGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAActc                                      <  2:2371394/150‑1 (MQ=255)

                                                                                                                 tatGCAGCGTTTTACCTGGCAACGCTCGGCGTAGCTAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAActct                                     <  1:1335386/150‑1 (MQ=255)

                                                                                                                 tatGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAActct                                     <  1:5265720/150‑1 (MQ=255)

                                                                                                                   tGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGt                                   >  1:1110454/1‑150 (MQ=255)

                                                                                                                   tGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGt                                   >  1:4186077/1‑150 (MQ=255)

                                                                                                                    gAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGtt                                  <  2:4446371/150‑1 (MQ=255)

                                                                                                                     aaGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGttt                                 <  1:2493647/150‑1 (MQ=255)

                                                                                                                     aaGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGttt                                 >  1:4098767/1‑150 (MQ=255)

                                                                                                                      aGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTc                                >  2:3447936/1‑150 (MQ=255)

                                                                                                                        cggtatACCTGGCCCCGCTCGGCGGAGCGAAATCTCAGGGCCTTGACGATCACATTGGCACCTAATTACCAGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACATCCACTCCGCTACAGCAGCTGCGCTATTACAACTCTGTTTCtt                              >  2:2906414/6‑150 (MQ=255)

                                                                                                                         gTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCttt                             >  1:1269492/1‑150 (MQ=255)

                                                                                                                            ttACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGt                          <  1:184724/150‑1 (MQ=255)

                                                                                                                             tACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTc                         >  2:3206057/1‑150 (MQ=255)

                                                                                                                             tACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTc                         <  2:4796787/150‑1 (MQ=255)

                                                                                                                             tACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTc                         >  2:3074994/1‑150 (MQ=255)

                                                                                                                             tACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTc                         >  1:2235202/1‑150 (MQ=255)

                                                                                                                               ccTGGCCACGCTCGGCGGAGCGAAATCGCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGa                       >  1:2423022/1‑150 (MQ=255)

                                                                                                                                cTGGCCACGCTCGGCGGAGCTAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGAc                      >  2:2323668/1‑150 (MQ=255)

                                                                                                                                 tGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACa                     <  1:2942746/150‑1 (MQ=255)

                                                                                                                                     cACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAAtt                 <  1:4742074/150‑1 (MQ=255)

                                                                                                                                     cACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAAtt                 <  1:2019374/150‑1 (MQ=255)

                                                                                                                                      aCGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTg                <  1:2466786/150‑1 (MQ=255)

                                                                                                                                        gCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGtt              >  2:3981859/1‑150 (MQ=255)

                                                                                                                                        gCTAGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATAGGCAACTTTTTACCTGGCAAAGATGCCGAATTCGATGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATAGtt              >  2:2232742/1‑150 (MQ=255)

                                                                                                                                          tCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAAATTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCg            >  1:676188/1‑150 (MQ=255)

                                                                                                                                             gcggAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtga         <  1:1656722/150‑1 (MQ=255)

                                                                                                                                              cggAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtgat        <  1:145159/150‑1 (MQ=255)

                                                                                                                                               ggAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtgatg       >  1:1557706/1‑150 (MQ=255)

                                                                                                                                               ggAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtgatg       <  2:2830959/150‑1 (MQ=255)

                                                                                                                                               ggAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACACCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtgatg       >  1:4463189/1‑150 (MQ=255)

                                                                                                                                                gAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtgatga      >  1:3592181/1‑150 (MQ=255)

AAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAAAGTATTGCAATTACAAGGCTATCGCCTCTCGGCATATGAAGCGTTTTACCTGGCCACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGAC  >  CP009273/3020080‑3020377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`