Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,044,493 A→C 5.2% L395W (TTG→TGG)  ubiI ← 2‑octaprenylphenol hydroxylase, FAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,044,4930AC 5.2% 1210.6 / 41.4 336L395W (TTG→TGG) ubiI2‑octaprenylphenol hydroxylase, FAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (164/153);  new base C (11/8);  total (175/161)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 344 total aligned reads displayed.

AATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATC  >  CP009273/3044344‑3044642
                                                                                                                                                     |                                                                                                                                                     
   ggCATTAGAAAATATAATGCGAAAACGGCGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAAt                                                                                                                                                    <  1:2127306/150‑1 (MQ=255)
    gCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATc                                                                                                                                                   >  2:1648786/1‑150 (MQ=255)
     cATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCg                                                                                                                                                  >  1:642731/1‑150 (MQ=255)
     cATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCg                                                                                                                                                  >  1:1546499/1‑150 (MQ=255)
      aTTAGAAAATATAATGCGAAAACGGAGTTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGt                                                                                                                                                 <  2:4822512/150‑1 (MQ=255)
        tAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGttt                                                                                                                                               >  1:5005557/1‑150 (MQ=255)
        tAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGttt                                                                                                                                               <  2:224602/150‑1 (MQ=255)
         aGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCCAATCGTTTa                                                                                                                                              >  2:3942641/1‑150 (MQ=255)
           aaaaTATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCACATCGTTTAAt                                                                                                                                            >  2:4674849/1‑150 (MQ=255)
              atatAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATccc                                                                                                                                         >  1:4782871/1‑150 (MQ=255)
              atatAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATccc                                                                                                                                         <  1:4321313/150‑1 (MQ=255)
                  aaTGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTg                                                                                                                                     >  2:317910/1‑150 (MQ=255)
                   aTGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGc                                                                                                                                    <  1:4123916/150‑1 (MQ=255)
                     gCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTGATCCCATTGCCt                                                                                                                                  <  1:3653890/150‑1 (MQ=255)
                     gCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCt                                                                                                                                  <  1:3231394/150‑1 (MQ=255)
                      cGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTg                                                                                                                                 <  2:2468927/150‑1 (MQ=255)
                         aaaCGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCg                                                                                                                              >  1:3857563/1‑150 (MQ=255)
                          aaCGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCgg                                                                                                                             <  2:4192404/150‑1 (MQ=255)
                             ggAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATa                                                                                                                          >  1:3152140/1‑150 (MQ=255)
                               aGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAg                                                                                                                        >  2:1241086/1‑150 (MQ=255)
                               aGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAg                                                                                                                        >  1:4212783/1‑150 (MQ=255)
                                   gAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATACACACCAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGc                                                                                                                    <  2:3686051/150‑1 (MQ=255)
                                    aaaTTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCg                                                                                                                   >  2:1773585/1‑150 (MQ=255)
                                      aTTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGCTAAGTTGCGGc                                                                                                                 >  1:4424654/1‑150 (MQ=255)
                                      aTTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGc                                                                                                                 >  1:607325/1‑150 (MQ=255)
                                      aTTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGc                                                                                                                 >  2:3463079/1‑150 (MQ=255)
                                       ttAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCt                                                                                                                <  2:901615/150‑1 (MQ=255)
                                       ttAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCAGTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCt                                                                                                                >  2:2374650/1‑150 (MQ=255)
                                        tAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCtt                                                                                                               <  1:3615657/150‑1 (MQ=255)
                                         aGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTa                                                                                                              <  1:1931623/150‑1 (MQ=255)
                                               tttCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTCACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCa                                                                                                        >  2:3524425/1‑150 (MQ=255)
                                               tttCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCa                                                                                                        <  2:4712684/150‑1 (MQ=255)
                                               tttCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCa                                                                                                        <  1:1672462/150‑1 (MQ=255)
                                                 tCAACTGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAgg                                                                                                      <  2:4472290/150‑1 (MQ=255)
                                                 tCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAgg                                                                                                      <  1:1820921/150‑1 (MQ=255)
                                                      tGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCg                                                                                                 <  2:3925191/150‑1 (MQ=255)
                                                      tGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCg                                                                                                 <  2:1587210/150‑1 (MQ=255)
                                                       gAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCgt                                                                                                <  1:2872802/150‑1 (MQ=255)
                                                         ggAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCgtgt                                                                                              <  2:5005557/150‑1 (MQ=255)
                                                             aaTCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGc                                                                                          >  2:378952/1‑150 (MQ=255)
                                                             aaTCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGc                                                                                          >  2:2701017/1‑150 (MQ=255)
                                                               tctcCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCa                                                                                        >  1:4480977/1‑150 (MQ=255)
                                                                ctcCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAg                                                                                       >  2:4292156/1‑150 (MQ=255)
                                                                   ccGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGttt                                                                                    <  1:5128407/150‑1 (MQ=255)
                                                                    cGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTc                                                                                   >  1:1019916/1‑150 (MQ=255)
                                                                    cGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTc                                                                                   >  1:5270180/1‑150 (MQ=255)
                                                                    cGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTc                                                                                   >  1:1302534/1‑150 (MQ=255)
                                                                        gAAAAAACCGTGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAAc                                                                               <  2:5186110/150‑1 (MQ=255)
                                                                         aaaaaaCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAAcc                                                                              >  2:835363/1‑150 (MQ=255)
                                                                         aaaaaaCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAAcc                                                                              >  2:2412000/1‑150 (MQ=255)
                                                                         aaaaaaCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAAATTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAAcc                                                                              <  2:2897462/150‑1 (MQ=255)
                                                                            aaaCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAat                                                                           <  1:5056846/150‑1 (MQ=255)
                                                                            aaaCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAat                                                                           <  1:4527614/150‑1 (MQ=255)
                                                                                cGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCCAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCa                                                                       >  1:3926891/1‑150 (MQ=255)
                                                                                cGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCa                                                                       <  2:3022287/150‑1 (MQ=255)
                                                                                cGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCa                                                                       <  2:537331/150‑1 (MQ=255)
                                                                                 gggAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCCAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCAc                                                                      >  2:284767/1‑150 (MQ=255)
                                                                                 gggAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCAc                                                                      >  2:1400892/1‑150 (MQ=255)
                                                                                    agaTGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgca                                                                   >  2:4121804/1‑150 (MQ=255)
                                                                                     gaTGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgcag                                                                  <  1:4575324/150‑1 (MQ=255)
                                                                                       tGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgcagca                                                                >  2:2037043/1‑150 (MQ=255)
                                                                                          aaGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCCAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGtt                                                             <  2:1640853/150‑1 (MQ=255)
                                                                                           aGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGttt                                                            <  1:4387504/150‑1 (MQ=255)
                                                                                            gtgtGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGtttt                                                           >  2:1008597/1‑150 (MQ=255)
                                                                                              gtgATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGtttttt                                                         <  2:1833728/150‑1 (MQ=255)
                                                                                                   gggTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCg                                                    <  2:4693428/150‑1 (MQ=255)
                                                                                                   gggTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCg                                                    >  2:2515817/1‑150 (MQ=255)
                                                                                                   gggTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCg                                                    <  2:3420878/150‑1 (MQ=255)
                                                                                                    ggTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCgg                                                   <  1:2201319/150‑1 (MQ=255)
                                                                                                       aTCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCATTTTTTTCGCCGGAtt                                                >  1:299628/1‑150 (MQ=255)
                                                                                                       aTCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCATTTTTTTCGCCGGAtt                                                >  1:298925/1‑150 (MQ=255)
                                                                                                          aaaTAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGt                                             >  2:955633/1‑150 (MQ=255)
                                                                                                          aaaTAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGt                                             <  1:1241086/150‑1 (MQ=255)
                                                                                                            aTAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGAGTGGTAc                                           >  1:2634732/1‑150 (MQ=255)
                                                                                                             tAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTAcc                                          <  1:1170073/150‑1 (MQ=255)
                                                                                                              aaaCAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGAAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTATAGCAAGGAAGCGTGTCGGACAGTTTCAATCCAATATGACGCAGCTGTTTTTGCGGCGGATTGGttacg                                         >  2:2933584/1‑146 (MQ=255)
                                                                                                               aacaacAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCgg                                        >  1:3393784/1‑150 (MQ=255)
                                                                                                                  aacaGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGaaa                                     <  2:3202996/150‑1 (MQ=255)
                                                                                                                   acaGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGaaaa                                    >  2:2597353/1‑150 (MQ=255)
                                                                                                                    caGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAAc                                   <  1:1143003/150‑1 (MQ=255)
                                                                                                                    caGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAAc                                   <  2:1885153/150‑1 (MQ=255)
                                                                                                                       cggagAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGa                                <  2:1464624/149‑1 (MQ=255)
                                                                                                                       aggagAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGa                                >  1:2440078/1‑150 (MQ=255)
                                                                                                                         gagaAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATc                              <  1:4651257/150‑1 (MQ=255)
                                                                                                                         gagaAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATc                              >  1:4266285/1‑150 (MQ=255)
                                                                                                                            aaaTTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATcgcg                           >  1:3169572/1‑150 (MQ=255)
                                                                                                                              aTTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCTGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGa                         >  2:5097752/1‑150 (MQ=255)
                                                                                                                               tttttAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGaa                        <  1:4674849/150‑1 (MQ=255)
                                                                                                                               tttttAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGaa                        >  1:2073152/1‑150 (MQ=255)
                                                                                                                                ttttAACGCAGCCATTCAGGCCAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAAt                       <  2:5226947/150‑1 (MQ=255)
                                                                                                                                  ttAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATcc                     <  2:780767/150‑1 (MQ=255)
                                                                                                                                    aaCGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCt                   >  1:4879441/1‑150 (MQ=255)
                                                                                                                                       gCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCa                <  1:4719547/150‑1 (MQ=255)
                                                                                                                                          gCCATTCAGGCCAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATAc             >  2:4810316/1‑150 (MQ=255)
                                                                                                                                          gCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATAc             >  1:900515/1‑150 (MQ=255)
                                                                                                                                           ccATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATAcc            <  2:3094084/150‑1 (MQ=255)
                                                                                                                                           ccATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATAcc            <  1:3525807/150‑1 (MQ=255)
                                                                                                                                            cATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATAcca           <  1:2317207/150‑1 (MQ=255)
                                                                                                                                                 aGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATAccagccag      <  2:1328955/150‑1 (MQ=255)
                                                                                                                                                     aaaTCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATc  >  1:537964/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATGGCATTAGAAAATATAATGCGAAAACGGAGGTGAAATTAGTTTATTTCAAATGAGGAAAATCTCCCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATC  >  CP009273/3044344‑3044642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑