Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,480 T→G 7.8% T418P (ACG→CCG)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4800TG 7.8% 802.4 / 9.3 282T418P (ACG→CCG) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (82/164);  new base G (24/5);  total (108/174)
Fisher's exact test for biased strand distribution p-value = 6.12e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

TGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGC  >  CP009273/3721331‑3721629
                                                                                                                                                     |                                                                                                                                                     
tGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGt                                                                                                                                                       <  2:4579342/150‑1 (MQ=255)
 ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCGCCCCGtg                                                                                                                                                      >  1:1974892/1‑149 (MQ=255)
 ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTa                                                                                                                                                      <  2:766217/150‑1 (MQ=255)
 ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTa                                                                                                                                                      <  2:3509002/150‑1 (MQ=255)
  gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTACCGGTAGTTGCGGCAACACGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTAc                                                                                                                                                     <  2:3647004/150‑1 (MQ=255)
  gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTAc                                                                                                                                                     <  2:905342/150‑1 (MQ=255)
   cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACg                                                                                                                                                    <  1:3371226/150‑1 (MQ=255)
     gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACggg                                                                                                                                                  >  1:3831670/1‑149 (MQ=255)
              cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgct                                                                                                                                         <  1:4404834/150‑1 (MQ=255)
              cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgct                                                                                                                                         <  1:5222021/150‑1 (MQ=255)
                tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCCCCCCCGGACGGTGATCGCgctgc                                                                                                                                       >  2:4844950/1‑150 (MQ=255)
                tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgc                                                                                                                                       <  1:3600599/150‑1 (MQ=255)
                     cTGCGATTCTGGTAGATGCTACTGTTCTAACGGTAGTTGCGGCAACATTTCAATGTGCGATTTCTCTGTATTCGCCGGGATCTGCGCCAGCCTTGCTGCGCCCTGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGAcc                                                                                                                                  <  1:1969704/150‑1 (MQ=255)
                           aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGTGCGGTAATCGGGCTGCGGTCCggggat                                                                                                                            >  1:1685088/1‑145 (MQ=255)
                             cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                                                          <  2:3139143/150‑1 (MQ=255)
                               ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTGATCGAGCTGCTGTCCGCTGTGATcc                                                                                                                        >  2:4541473/1‑150 (MQ=255)
                               ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                        <  1:1520664/150‑1 (MQ=255)
                               ggTAGATGCGACTGTACTAACGGTAGTTGCGGCAACTATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                        <  2:3818179/150‑1 (MQ=255)
                                   gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCa                                                                                                                    >  1:3144750/1‑150 (MQ=255)
                                   gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCGCGTCCGGGAAGGGGGCTGGTGGCCGGGGTTATCCggct                                                                                                                    >  2:5452928/1‑147 (MQ=255)
                                       cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCGCCCGGACGGGGCGGGGGGTGCTGGCCGCGGATGTCCGCCGGcgg                                                                                                                >  2:1988419/1‑148 (MQ=255)
                                        gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGTTCGGTAATCGAGGTGCTGGCGGCGGGTGTCCGCCGGCATc                                                                                                               >  1:2207382/1‑150 (MQ=255)
                                         aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                              <  2:3844457/150‑1 (MQ=255)
                                          cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTg                                                                                                             <  2:4782756/150‑1 (MQ=255)
                                            gTTCTAACGGTAGTTGCGGCAACAATTCAATGATCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                                           <  2:1764868/150‑1 (MQ=255)
                                              tCTAACGGTAGTTGCGGCAACAATTCAATGATCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTTCGCCCAGTGCTCTCCCCACATCCCCCCCCCAGAGGGAGTCGAGCAGCTGACGGCTGAACGCCGCCAGGATCTCACGc                                                                                                         >  2:1423895/1‑150 (MQ=255)
                                              tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCCCCCCCGTGCGGTCAGCGAGCTGCGGACCGCTGAGATCCGCCCGCATCTGACGc                                                                                                         >  2:2498229/1‑150 (MQ=255)
                                              tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGGAATCGAGCTGCTGACCGCGGGGGTCCGCCAGCATCTGACgg                                                                                                         >  1:2501870/1‑149 (MQ=255)
                                               cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                                                        <  1:5126867/150‑1 (MQ=255)
                                                tAACGGTAGTTGCGGCCACAATTCAATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                                                       <  2:3276229/150‑1 (MQ=255)
                                                tAACGGTAGTTGCGGCAACAATTCAATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                                                       <  2:678090/150‑1 (MQ=255)
                                                 aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGGACTCGGGCTGCTGGCCGCGGAGATCCGCCGGCGGCTGGCGcccg                                                                                                      >  2:1598121/1‑148 (MQ=255)
                                                 aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGGACGCGAGCTGCTGGCCGGTGGTATCCGCGAGCAGCTggggcccg                                                                                                      >  2:1598177/1‑143 (MQ=255)
                                                 aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                                                      <  1:5123007/150‑1 (MQ=255)
                                                    ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTCAGCGCGCTGCTGACCGCGGGGATCCGCCCGCATCTGACGcccggcc                                                                                                   >  2:4223658/1‑145 (MQ=255)
                                                     gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                                                  <  2:1141131/150‑1 (MQ=255)
                                                     gTAGTTGCGCCAACCAGTCAATGAGCGATTTCGCTGGATGCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCACCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                                                  <  2:5290750/150‑1 (MQ=255)
                                                      tAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGGGCGGTAGTCGGGGTGGTGGCCGGGGTTGGCCGCCAGCATCTGGCGCCGGTAGTc                                                                                                 >  1:772979/1‑150 (MQ=255)
                                                       aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCGCCCCCGTGGGGTCATGGGGCTGCGGTCCGGTGATAGCGGCGAGCATCTGACGCCAGTGCTct                                                                                                >  2:4693802/1‑149 (MQ=255)
                                                        gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                               <  2:633226/150‑1 (MQ=255)
                                                        gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                               <  2:2662064/150‑1 (MQ=255)
                                                           gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                            <  2:691425/150‑1 (MQ=255)
                                                           gCGGCAACAATTCAATGAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                            <  2:1943968/150‑1 (MQ=255)
                                                            cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcg                                                                                           <  1:2598819/150‑1 (MQ=255)
                                                               caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTCCGGTAATCGTGCTGCTGTCCGCTGATGGCCGCCGGCATCTGACGCGAGTTCTCAGTAcgcgg                                                                                        >  1:2874984/1‑149 (MQ=255)
                                                                aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                                                       >  2:1022542/1‑150 (MQ=255)
                                                                aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                                                       <  2:529956/150‑1 (MQ=255)
                                                                 acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                                                      <  1:3806536/150‑1 (MQ=255)
                                                                       cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                                <  1:4148656/150‑1 (MQ=255)
                                                                       cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                                <  1:2176571/150‑1 (MQ=255)
                                                                        aaTGAGCGAGTCCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                                                                               <  2:2569672/150‑1 (MQ=255)
                                                                           gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                                            <  1:4948202/150‑1 (MQ=255)
                                                                            aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCCGCATCTGACGCCAGTACGCACTACGCGCCCCGCCCCCacgc                                                                           >  2:2862689/1‑147 (MQ=255)
                                                                               gcagtctcTGGATTCGCCGCGCTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTACGGTAATCGAGCGGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                        <  2:4733114/146‑1 (MQ=255)
                                                                               gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                        <  1:673860/150‑1 (MQ=255)
                                                                               gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGGTGACCGCTGGTCTCCGCCCGCATCTGAGGGCAGTGCTGCCGGCGCGCCCCGCCCCCaagccac                                                                        >  1:1446335/1‑145 (MQ=255)
                                                                               gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACGCAAc                                                                        >  1:4092179/1‑150 (MQ=255)
                                                                               catctctcTGGATCCGCCGCGATCTGCCCCAGCCTAGCGGCTCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTCCTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCacc                                                                        <  2:3975384/146‑3 (MQ=255)
                                                                                 tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                          >  1:3016981/1‑114 (MQ=255)
                                                                                 tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                          <  2:258048/114‑1 (MQ=255)
                                                                                 tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                          <  2:3016981/114‑1 (MQ=255)
                                                                                    ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac                                                                   <  1:977621/150‑1 (MQ=255)
                                                                                     tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAGGTAaca                                                                  >  2:432139/1‑150 (MQ=255)
                                                                                      ctGGATTCGCCGCGATCTGCGCCAGCCTAGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACTGTAATCGAGCTGCTGACCGATGATATCAGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAacac                                                                 <  2:3307344/150‑1 (MQ=255)
                                                                                      ccggACTCGCCCCGATCTGCGCCAGCCTCGCTGCGCCCAGGGCTGCCCCCACCTCCCCCCCCGAACCGTCATCGAGCCGCTGACCGATGATATCCCCCAGCAACAGACTCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAacac                                                                 <  2:4419382/148‑1 (MQ=255)
                                                                                         gagtCGCCGCTATCGTCGCCAGCCTTGCGGCGCCCTCTTCTGGCCCCACATCCCACGCCGGTCGGTAATCGAGCTGCTGACCGCTGATAACGGCCAGCATCTGTAGCGAGATCTCACTACGCGCCCCGCCCCCAATCACCGTAACACttt                                                              <  2:5080077/147‑1 (MQ=255)
                                                                                         gaatCGCCGCGATCGCCGCCACCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCTTACGGTAAGCGAGCTGCTGACCGATGACATCCGCCAGCATATGACGCCAGTACCCACTACGCGCCCCGCCCCCAATCAACGTAACACgtt                                                              <  2:2283088/147‑1 (MQ=255)
                                                                                            tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGTACGGGGAGCGTGCTGCGGACCGCGGATCGCCGCCAGCCTCTGGCGCCGGGGCGCACTTCGGGCCCCGCCCCCGCGCCACGTAGCCCTTTggg                                                           >  2:139296/1‑148 (MQ=255)
                                                                                             cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg                                                          <  1:4620844/150‑1 (MQ=255)
                                                                                               ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                        <  1:413585/150‑1 (MQ=255)
                                                                                                 gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa                                                      <  1:1732640/150‑1 (MQ=255)
                                                                                                 gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATACCCCCCCGTACGGTAATCGAGGTGGTGACCGCTGATATCCGCCAGCATCTGAAGGCAGTACTCACGTCGCGCCCCGCCCCCAATCAACGGTACAAGTTGCGGtttc                                                      >  1:841114/1‑149 (MQ=255)
                                                                                                   gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                    <  1:5447264/150‑1 (MQ=255)
                                                                                                   gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                    <  1:5416508/150‑1 (MQ=255)
                                                                                                    aTCTTCGACATCCTTGATGATCCCAGTGCTGGCCCCACATCCCCCCCCGTAATGTAATCGAGCTTCTGACAGCTGATATCCGCCAGCATCTGACTCCAGTACTCACTACGCTCCCCTACCCCATTCAACGTAACTCTTTTCGGTTTtata                                                   <  2:2080383/150‑1 (MQ=255)
                                                                                                     tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                  <  1:1940828/150‑1 (MQ=255)
                                                                                                         cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCTTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCGATCAACGTAACACTTTGCGGTTTAGTGCCGCa                                              >  1:1151419/1‑150 (MQ=255)
                                                                                                                   gctgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg                                    <  1:1504304/150‑1 (MQ=255)
                                                                                                                      gcgcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                 <  1:4164944/150‑1 (MQ=255)
                                                                                                                       cgcCCAGTGCTGGCCCCCCCTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACAt                                <  2:1839397/150‑1 (MQ=255)
                                                                                                                       cgcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACAt                                <  1:1438043/150‑1 (MQ=255)
                                                                                                                        gcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                               >  1:2391581/1‑150 (MQ=255)
                                                                                                                        ccccAGCGCGGGCCCCACCTCCCCCCCCGTCCCGGCAGCGCGCCGCCGACCGCTGCGACCCGCCCGCATCTGCCGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGGACCACTTTGCGGTTTCCTCCCGCAGGCAGGCCCGCCacc                               <  2:2027431/149‑3 (MQ=255)
                                                                                                                          ccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                             <  1:353024/150‑1 (MQ=255)
                                                                                                                           cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                            <  1:2338245/150‑1 (MQ=255)
                                                                                                                           cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                            <  1:2942472/150‑1 (MQ=255)
                                                                                                                            aGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg                           <  1:1058754/150‑1 (MQ=255)
                                                                                                                               gCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCa                        <  1:5328090/150‑1 (MQ=255)
                                                                                                                               gCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCa                        <  1:2619814/150‑1 (MQ=255)
                                                                                                                                   gCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTg                    <  1:1349520/150‑1 (MQ=255)
                                                                                                                                    ccccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGc                   <  1:3331611/150‑1 (MQ=255)
                                                                                                                                    ccccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGc                   <  1:3331725/150‑1 (MQ=255)
                                                                                                                                     cccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                  <  1:4844950/150‑1 (MQ=255)
                                                                                                                                      ccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa                 <  1:2326025/150‑1 (MQ=255)
                                                                                                                                          aTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcgc             >  2:978041/1‑150 (MQ=255)
                                                                                                                                            ccccccccGTACGGTAATCGCTCTGATGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCCACGTAACCCTTTGCGGTTTACTACCGCAGGCATGCACGACCTCCATGCCATCTGCCCGCGCAt           <  2:2143195/150‑1 (MQ=255)
                                                                                                                                               cccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAc        <  1:5058691/150‑1 (MQ=255)
                                                                                                                                                    gTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACg   >  1:1863162/1‑150 (MQ=255)
                                                                                                                                                     tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGc  >  2:1288176/1‑150 (MQ=255)
                                                                                                                                                     tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGc  >  2:4018610/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGC  >  CP009273/3721331‑3721629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑