Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,485 T→G 8.3% Y416S (TAC→TCC)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4850TG 8.3% 709.8 / 12.0 270Y416S (TAC→TCC) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (81/157);  new base G (23/6);  total (104/166)
Fisher's exact test for biased strand distribution p-value = 3.62e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCC  >  CP009273/3721336‑3721634
                                                                                                                                                     |                                                                                                                                                     
gttAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGt                                                                                                                                                       <  2:1208680/148‑1 (MQ=255)
gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGt                                                                                                                                                       <  1:3392956/150‑1 (MQ=255)
      cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCCGCCGCCCCCGGACGGTGAGCGa                                                                                                                                                 >  1:260215/1‑150 (MQ=255)
        gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGGAAGCGGgc                                                                                                                                               >  2:2267244/1‑150 (MQ=255)
         cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgct                                                                                                                                              <  2:5175022/150‑1 (MQ=255)
           tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgc                                                                                                                                            <  2:5298664/150‑1 (MQ=255)
           tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgc                                                                                                                                            <  1:3600599/150‑1 (MQ=255)
             aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGGAGGGTAATCGAGctgctg                                                                                                                                          >  2:450774/1‑150 (MQ=255)
                cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGAcc                                                                                                                                       <  1:1969934/150‑1 (MQ=255)
                 tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                                                                                      <  1:2814283/150‑1 (MQ=255)
                 tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGCAGCGAGCTGCTGTCCg                                                                                                                                      >  2:3041244/1‑150 (MQ=255)
                   cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCGCGTCCGGTGATCGGGCTGCGGGCCggg                                                                                                                                    >  2:4813033/1‑148 (MQ=255)
                      aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGTGCGGTAATCGGGCTGCGGTCCggggat                                                                                                                                 >  1:1685088/1‑145 (MQ=255)
                         tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATc                                                                                                                              <  1:5461139/150‑1 (MQ=255)
                          ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                             <  1:1520664/150‑1 (MQ=255)
                           gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCg                                                                                                                            <  2:730647/150‑1 (MQ=255)
                              gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCa                                                                                                                         >  1:3144750/1‑150 (MQ=255)
                              gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                                         <  1:1838442/150‑1 (MQ=255)
                              gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                                        >  2:678895/1‑150 (MQ=255)
                              gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                                        >  2:4383264/1‑150 (MQ=255)
                                  cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCGCCCGGACGGGGCGGGGGGTGCTGGCCGCGGATGTCCGCCGGcgg                                                                                                                     >  2:1988419/1‑148 (MQ=255)
                                    aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                                   <  2:2955965/150‑1 (MQ=255)
                                    aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                                   <  2:3844457/150‑1 (MQ=255)
                                     cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTg                                                                                                                  <  2:846214/150‑1 (MQ=255)
                                      tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCGCCGTACGGGAATCGGGCTGCGGACCGGTGAGAGCCGCCAGCATCTgt                                                                                                                 >  1:2957368/1‑149 (MQ=255)
                                       gTTCTAACGGTAGTTGCGGCAACAATTCAATGATCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                                                <  2:1764868/150‑1 (MQ=255)
                                         tCTAACGGTAGTTGCGGCAACAATTCAATGATCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTTCGCCCAGTGCTCTCCCCACATCCCCCCCCCAGAGGGAGTCGAGCAGCTGACGGCTGAACGCCGCCAGGATCTCACGc                                                                                                              >  2:1423895/1‑150 (MQ=255)
                                         tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGGCCCCCCCGTACGGTCATCGGGCTGCGGACCGCGGATATCCGCCAGCATCTGAGGc                                                                                                              >  2:2498208/1‑150 (MQ=255)
                                          cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                                                             <  1:5126780/150‑1 (MQ=255)
                                            aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGGACTCGGGCTGCTGGCCGCGGAGATCCGCCGGCGGCTGGCGcccg                                                                                                           >  2:1598121/1‑148 (MQ=255)
                                            aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGGACGCGAGCTGCTGGCCGGTGGTATCCGCGAGCAGCTggggcccg                                                                                                           >  2:1598177/1‑143 (MQ=255)
                                              cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGAATCTGACGCCAGTa                                                                                                         <  1:443773/150‑1 (MQ=255)
                                               ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTCAGCGCGCTGCTGACCGCGGGGATCCGCCCGCATCTGACGcccggcc                                                                                                        >  2:4223658/1‑145 (MQ=255)
                                                  cgTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACTTCCCCCCCCGTCCGGTCATCGAGCGGCTGACCGCTGATATCCGCCCGCCTCTGACGCCCGtgcgca                                                                                                     >  2:3260194/2‑145 (MQ=255)
                                                  aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCGCCCCCGTGGGGTCATGGGGCTGCGGTCCGGTGATAGCGGCGAGCATCTGACGCCAGTGCTct                                                                                                     >  2:4693802/1‑149 (MQ=255)
                                                  aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCa                                                                                                     <  1:2148530/150‑1 (MQ=255)
                                                   gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                                    <  2:2662055/150‑1 (MQ=255)
                                                   gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGATGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                                    >  2:2851710/1‑150 (MQ=255)
                                                      gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                                 <  1:2331872/150‑1 (MQ=255)
                                                      gCGGCAACAATTCAATGAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                                 <  2:1943968/150‑1 (MQ=255)
                                                         gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgcg                                                                                              <  1:4150089/150‑1 (MQ=255)
                                                          caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGTCCGCCCCCGTGCGGTGATCGGGGTGCTGGCCGCTGTGTTCCGCCGGCTGCTGACGCGAGTGCTGAGTAcgcgg                                                                                             >  1:2874995/1‑149 (MQ=255)
                                                           aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                                                            >  2:1022542/1‑150 (MQ=255)
                                                            acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                                                           <  1:3806536/150‑1 (MQ=255)
                                                                   aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTGAGCGTGCTGCTGACCGCTGAGATCCGCCAGCAGCTGACGCCAGTACTCAGTACGCGCCCCGccccc                                                                                    >  2:4574794/1‑150 (MQ=255)
                                                                   aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGGAGGGGGCTGCTGACCGGTGATATCCGCCCGCATGTGACGCCGGTGCTGCGTGCGCGCCCCGccccc                                                                                    >  2:477037/1‑150 (MQ=255)
                                                                   aaTGAGCGAGTCCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                                                                                    <  2:2569672/150‑1 (MQ=255)
                                                                     tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGTGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGcccccga                                                                                  >  2:655887/1‑148 (MQ=255)
                                                                      gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                                                 <  1:4948202/150‑1 (MQ=255)
                                                                       aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCGCCGGAGGGGGATCGTGCTGCGGTCCGGGGGGTGCCGCCGGCATCGGTCGCCAGTGCGCACTACGCGCCCCGCCCCCCATc                                                                                >  1:682502/1‑150 (MQ=255)
                                                                         cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCATGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCTCCATCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCac                                                                              <  2:590251/150‑2 (MQ=255)
                                                                          gcttccgctGGATTCGCCGCGCTCGGCGCCAGCCTAGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTCCGGTCATCGAGCTGCTGACCTCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                             <  2:4733112/143‑1 (MQ=255)
                                                                          gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGGTGACCGCTGGTCTCCGCCCGCATCTGAGGGCAGTGCTGCCGGCGCGCCCCGCCCCCaagccac                                                                             >  1:1446335/1‑145 (MQ=255)
                                                                          gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACGCAAc                                                                             >  1:4092179/1‑150 (MQ=255)
                                                                          catctctcTGGATCCGCCGCGATCTGCCCCAGCCTAGCGGCTCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTCCTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCacc                                                                             <  2:3975384/146‑3 (MQ=255)
                                                                            tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                               >  1:258048/1‑114 (MQ=255)
                                                                            tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                               >  1:3016981/1‑114 (MQ=255)
                                                                            tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                               <  2:257229/114‑1 (MQ=255)
                                                                              tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCATCCCCCCCCGTACGGGAATCGTGCTGCTGACCGCTGATGTCCGCCAGCATGTGTCGCCGGTTCTCACTACGCGCCCCGCCCCCCGTCAACGTag                                                                         >  2:1335457/1‑149 (MQ=255)
                                                                              tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa                                                                         <  1:3848763/150‑1 (MQ=255)
                                                                               ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac                                                                        <  1:977475/150‑1 (MQ=255)
                                                                               ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac                                                                        <  1:5220678/150‑1 (MQ=255)
                                                                                    gatcccccgcGCTCTGCGCCAGCCTTGCGCCGCCCAGCGCTGGCCCCACATCCCCCCCCGCACCGTACTCGAGCTGCTGACCGCTGATAGCCGCCAGCACCTGACGCCAGGACCCACGACGCGCCCCGCCCCCCATCAACGTAACCCttt                                                                   <  2:3298679/144‑1 (MQ=255)
                                                                                    gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGAGCTCGCCCCACCTCCCCCCCAGTACGGTAAGCGCGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGACCCCAATCAACGTAACACttt                                                                   <  2:5436064/150‑1 (MQ=255)
                                                                                       tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGTACGGGGAGCGTGCTGCGGACCGCGGATCGCCGCCAGCCTCTGGCGCCGGGGCGCACTTCGGGCCCCGCCCCCGCGCCACGTAGCCCTTTggg                                                                >  2:139296/1‑148 (MQ=255)
                                                                                       tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                                                <  1:2221930/150‑1 (MQ=255)
                                                                                        cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg                                                               <  1:4620844/150‑1 (MQ=255)
                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                             <  1:2485866/150‑1 (MQ=255)
                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                             <  1:413585/150‑1 (MQ=255)
                                                                                           cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                            <  1:2199284/150‑1 (MQ=255)
                                                                                              gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                         <  1:5415341/150‑1 (MQ=255)
                                                                                               aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                                                        <  1:531621/150‑1 (MQ=255)
                                                                                                    cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCTTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCGATCAACGTAACACTTTGCGGTTTAGTGCCGCa                                                   >  1:1151419/1‑150 (MQ=255)
                                                                                                      ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCGGACCGCTGTTTTCCGCCAGCCTCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACCCTTTGCGGGTTAATACCGCAgg                                                 >  1:5418291/1‑150 (MQ=255)
                                                                                                         gCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                                              <  1:798556/150‑1 (MQ=255)
                                                                                                              gctgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg                                         <  1:1504304/150‑1 (MQ=255)
                                                                                                                ggcgcCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGCACCCGAGCCGCTGACCCCTGATATCCGCCAGCATCCGACGCCAGTACTCACCACGCGCCCCGCCCCCCCTCAACGTAACACTTTGCGGTTTAAGACCGCAGGCATGcccccc                                       <  2:4315005/149‑6 (MQ=255)
                                                                                                                  cgcCCAGTGCTGGCCCCCCCTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACAt                                     <  2:1839397/150‑1 (MQ=255)
                                                                                                                  cgcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACAt                                     <  1:1438043/150‑1 (MQ=255)
                                                                                                                   gcCCAGTGCCGCCCCCACATCCCCCCCCTCACGGCAACCGCGCTGCTGACCGCTGATATCCTCCAGCATCTGACCCCAGTACTCACTCCGCGCCCCGCCCCCAATCCACGTACCACTTTGCGGTTTAATACCGCAGGCACGCCCGAcccc                                    <  2:2033312/150‑4 (MQ=255)
                                                                                                                   ccccAGCGCGGGCCCCACCTCCCCCCCCGTCCCGGCAGCGCGCCGCCGACCGCTGCGACCCGCCCGCATCTGCCGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGGACCACTTTGCGGTTTCCTCCCGCAGGCAGGCCCGCCacc                                    <  2:2027431/149‑3 (MQ=255)
                                                                                                                     ccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                                  <  1:353024/150‑1 (MQ=255)
                                                                                                                      cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                                 <  1:4223658/150‑1 (MQ=255)
                                                                                                                       aGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg                                <  1:1058754/150‑1 (MQ=255)
                                                                                                                              gCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTg                         <  1:1349520/150‑1 (MQ=255)
                                                                                                                               ccccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGc                        <  1:3331725/150‑1 (MQ=255)
                                                                                                                                cccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                       >  1:1929407/1‑150 (MQ=255)
                                                                                                                                cccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                       <  1:4844950/150‑1 (MQ=255)
                                                                                                                                    naTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg                   >  2:3480573/2‑150 (MQ=255)
                                                                                                                                     aTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcgc                  >  2:978041/1‑150 (MQ=255)
                                                                                                                                       ccccccccGCACCGTACGCGAGCTGCCGACCGCTGAGAGCCGCCAGCATCCGCCGCCAGTACTCCCTCCGCGCCCCGCCCCCCCTCAACGTACCACTTTGCGGTTTCATACCGCAGGCCTGCCCGACATCCATGCCATCTGCCAGCGCAt                <  2:2142529/150‑1 (MQ=255)
                                                                                                                                        cccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTAACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa               >  2:5004545/1‑150 (MQ=255)
                                                                                                                                          cccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAc             <  1:5058691/150‑1 (MQ=255)
                                                                                                                                           ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAcc            <  1:2373489/150‑1 (MQ=255)
                                                                                                                                               gTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACg        >  1:1942011/1‑150 (MQ=255)
                                                                                                                                                tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGc       >  2:4018610/1‑150 (MQ=255)
                                                                                                                                                  cGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCt     <  1:894445/150‑1 (MQ=255)
                                                                                                                                                    gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCATGACATCCATGCCATCTGCCATCGCATAACCCACGCCTTc   <  1:509365/150‑1 (MQ=255)
                                                                                                                                                    gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCATGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc   <  1:3260194/150‑1 (MQ=255)
                                                                                                                                                     tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTcc  >  2:3842282/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCC  >  CP009273/3721336‑3721634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑