Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,952,817 T→G 6.9% intergenic (+12/+75) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,952,8170TG 6.9% 938.8 / 11.6 315intergenic (+12/+75)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (115/171);  new base G (26/3);  total (141/174)
Fisher's exact test for biased strand distribution p-value = 1.88e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 316 total aligned reads displayed.

CTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGG  >  CP009273/3952669‑3952966
                                                                                                                                                    |                                                                                                                                                     
cTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATg                                                                                                                                                      <  2:1623258/150‑1 (MQ=255)
 tATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGc                                                                                                                                                     >  1:3897213/1‑150 (MQ=255)
  aTTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGcc                                                                                                                                                    <  1:106866/150‑1 (MQ=255)
  aTTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGcc                                                                                                                                                    >  2:1953752/1‑150 (MQ=255)
  aTTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGcc                                                                                                                                                    >  2:3621195/1‑150 (MQ=255)
   ttCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGccc                                                                                                                                                   <  2:3030397/150‑1 (MQ=255)
    tCCGGAAGGCGCGGTAGATAACGGGCAGCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCt                                                                                                                                                  <  1:990715/150‑1 (MQ=255)
    tCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCt                                                                                                                                                  <  1:3664403/150‑1 (MQ=255)
       ggAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAc                                                                                                                                               >  2:5221399/1‑150 (MQ=255)
         aaGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAccc                                                                                                                                             >  2:732014/1‑150 (MQ=255)
          aGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAcccc                                                                                                                                            <  2:3477167/150‑1 (MQ=255)
          aGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACAcccc                                                                                                                                            >  1:98256/1‑150 (MQ=255)
           ggCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCg                                                                                                                                           <  2:4300717/150‑1 (MQ=255)
            gcgcGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGa                                                                                                                                          <  1:2218922/150‑1 (MQ=255)
             cgcgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCgcc                                                                                                                                         >  2:2901746/1‑148 (MQ=255)
                ggTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCAccca                                                                                                                                      >  1:4801835/1‑149 (MQ=255)
                     aTAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTccc                                                                                                                                 <  1:432713/150‑1 (MQ=255)
                     aTAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAACCCGACCCACTccc                                                                                                                                 >  1:1193900/1‑150 (MQ=255)
                      tAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCctcaacca                                                                                                                                >  1:5003053/1‑145 (MQ=255)
                         cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                             <  1:2460170/150‑1 (MQ=255)
                           ggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACaggg                                                                                                                           <  2:2425053/150‑1 (MQ=255)
                           ggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACaggg                                                                                                                           <  1:893484/150‑1 (MQ=255)
                             cAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggag                                                                                                                         <  2:5097119/150‑1 (MQ=255)
                               aCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACTCCCCCagggggag                                                                                                                       >  2:1405071/1‑150 (MQ=255)
                                cTGCGTGATGTGAACTAAGCGATTAGCTGCCTTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGTCTATATGTCAGATATGATACGTATTGCTGTTGTGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagagg                                                                                                                      <  2:1203011/150‑1 (MQ=38)
                                  gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggga                                                                                                                    <  2:3235133/150‑1 (MQ=255)
                                   cGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACagggagagggag                                                                                                                   >  1:2880185/1‑150 (MQ=255)
                                          tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTCTATGACAGATATGAAACGTATTGCTGTTGCGGGGTAAGTGCGCGCTGAGGCCCTCACCCCGACCCACTCCCCCAGGGAGAGGGCGAAAacac                                                                                                            >  1:1138044/1‑150 (MQ=255)
                                          tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAacac                                                                                                            <  1:2850523/150‑1 (MQ=255)
                                           gAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACt                                                                                                           <  2:4980299/150‑1 (MQ=255)
                                             aCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCa                                                                                                         <  2:2293098/150‑1 (MQ=255)
                                             aCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCa                                                                                                         <  2:2416672/150‑1 (MQ=255)
                                              cGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCGCTCCCACCCGGAGAGGGAGaaaaaaaacaa                                                                                                        >  1:2551825/1‑143 (MQ=255)
                                              cGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCACACCCACCGGGAGAGGGAGAAAACACTCaa                                                                                                        >  1:3633759/1‑150 (MQ=255)
                                                aaGAGAGTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAgg                                                                                                      <  2:4442816/150‑1 (MQ=255)
                                                     aTTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtc                                                                                                 <  1:4567169/150‑1 (MQ=255)
                                                      ttCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtct                                                                                                <  2:3415817/150‑1 (MQ=255)
                                                       tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtctc                                                                                               <  1:922456/150‑1 (MQ=255)
                                                       tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtctc                                                                                               <  1:660022/150‑1 (MQ=255)
                                                        cGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTcc                                                                                              <  1:4666890/150‑1 (MQ=255)
                                                           aGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTgg                                                                                           >  2:1271351/1‑150 (MQ=255)
                                                           aGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGATAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTgg                                                                                           <  2:2741771/150‑1 (MQ=255)
                                                            gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAACACACAAGGCCTTATCCTGga                                                                                          >  1:2918857/1‑150 (MQ=255)
                                                              cATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGgaga                                                                                        <  2:3545844/150‑1 (MQ=255)
                                                                tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAg                                                                                      <  1:1512292/150‑1 (MQ=255)
                                                                 gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAgg                                                                                     <  1:2626328/150‑1 (MQ=255)
                                                                  cGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGc                                                                                    <  2:5140693/150‑1 (MQ=255)
                                                                  cGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCACCCACACCCACAGGGAGAGGGAGAAAAAACAAAAGTCCCTCTCCTCGAGAAGGc                                                                                    >  1:5356245/1‑150 (MQ=255)
                                                                   gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCCTCTCCTGGAGAAGGcc                                                                                   <  1:5221399/150‑1 (MQ=255)
                                                                   gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCTCACCCACAGGGAGAGGGAGTAAACACTCAATGCCTTCTACTGGAGAATGcc                                                                                   >  1:2282569/1‑150 (MQ=255)
                                                                      tGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg                                                                                <  1:161991/150‑1 (MQ=255)
                                                                       gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGc                                                                               <  1:4068265/150‑1 (MQ=255)
                                                                         gCAGGTAGGTAAGAAACTGCGCGGCTATCTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTa                                                                             <  2:51913/150‑1 (MQ=255)
                                                                         gCAGGGAGGTAAGACACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCCCAGGTAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTa                                                                             <  2:4604385/150‑1 (MQ=255)
                                                                           aggtaggtAAGAACCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAtt                                                                           <  2:2208649/150‑1 (MQ=255)
                                                                           aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAtt                                                                           <  1:3173862/150‑1 (MQ=255)
                                                                              taggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGt                                                                        <  1:560693/150‑1 (MQ=255)
                                                                               aggtaAGACACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCGCCTGGAGAAGGCCTTGCTATTAGtt                                                                       <  2:3130078/150‑1 (MQ=255)
                                                                               aggtaAGAAACTGCGCGGCTATATGACAGATATGAAAAGTATTGCTGTTGCGGGTTAAGTGAGCGCTGAGGACCACAACCCGAAACACAAACACAAGGAGTGGGTGAAAAAACACAAGGCCATATCCAGGAGAAGGACTTTGTATTAttt                                                                       >  1:413764/1‑150 (MQ=255)
                                                                                 gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCACTCCCACAGGGAGAGGGAGAAAACAATCAATGCCTTCACCGGGAGAAGGCCATGCTATTAGtttc                                                                     >  1:4398379/1‑148 (MQ=38)
                                                                                     gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTa                                                                 >  2:1711083/1‑150 (MQ=255)
                                                                                      aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                                <  2:4850096/150‑1 (MQ=255)
                                                                                        aCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAg                                                              <  1:1884405/150‑1 (MQ=255)
                                                                                            cgcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAcc                                                          <  1:518751/150‑1 (MQ=255)
                                                                                              cgGCTATATGACAGATATGAAACGAATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACAACCCCACCCACACCCACACGGAGAGGGAGAAAACACAAAAGGCCTTCTCCTGGAGAAGGCCATGGTATTAGTTTCGGTACAGCAccat                                                        >  1:4296421/1‑148 (MQ=255)
                                                                                               ggCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCATCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTa                                                       >  2:2104851/1‑150 (MQ=255)
                                                                                               ggCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTAACCCCGAACCTCTACCACAGGGAGAGGGAGAAAAAACTCAAGGCCTTCTCCTGGAGAAGGGCGTGCTATTAGTTGCGGTACAGCACCTTa                                                       >  2:353834/1‑150 (MQ=255)
                                                                                                gCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa                                                      <  2:5357199/150‑1 (MQ=255)
                                                                                                gCTATATGACAGAGATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa                                                      <  2:3446813/150‑1 (MQ=255)
                                                                                                 cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCTCACCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCCCCTTCat                                                     >  2:4033868/1‑150 (MQ=255)
                                                                                                  tataTGACCGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatg                                                    <  1:1872735/150‑1 (MQ=255)
                                                                                                    tatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCTCTCCCACAGGGAGAGGGCGAAAACACTCAAGGCCATCTCCTTGAGAAGGCCATGCTATTAGTTGCGGTACAGCACCTTAatgag                                                  >  2:3274778/1‑149 (MQ=38)
                                                                                                     atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                 <  1:5145284/150‑1 (MQ=255)
                                                                                                      tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                <  1:1868190/150‑1 (MQ=255)
                                                                                                        aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                              <  2:4382265/150‑1 (MQ=255)
                                                                                                        aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                              <  2:4723387/150‑1 (MQ=255)
                                                                                                        aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                              <  2:1485446/150‑1 (MQ=255)
                                                                                                        aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCTCACCCACAGGGAGCGGGGGAAAACAAAAAAGGCCATATACTGGAGGAGGCCTTGCTAATATTTTCGGTACAGCACCTAAaagatttta                                              >  1:4923940/1‑146 (MQ=255)
                                                                                                            atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACACAAGGCCATCACCTGGAGAAGGCCTTGCTATTATTTGCGGTACAGCACCTTAATGAtgttgtata                                          >  2:2546795/1‑144 (MQ=38)
                                                                                                               tGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGa                                       <  2:844908/150‑1 (MQ=255)
                                                                                                                gAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCCCACCCCGAACCTCTCCCACAGTGAGAGGGGGAAAAAACACAAGGCCGTATCCGGGCGAAAGCCGTGCGATTATTTGCGGGACAGCACCAAAATTATGTGTTATACgga                                      >  2:1433843/1‑148 (MQ=255)
                                                                                                                   aCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTg                                   <  2:635747/150‑1 (MQ=255)
                                                                                                                    cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                  >  2:3957403/1‑150 (MQ=255)
                                                                                                                     gTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCGACCCTCTCCCCCAGGGAGAGGGCGAAAACACTCCAGGCCTTCTCCTGGGGCAGGCCTTGGGATTAGTTGCGGTCCAGCACCATAATGAAGTGATATCCGAACTggg                                 >  2:558443/1‑150 (MQ=255)
                                                                                                                          gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACAATCAAGGCCTTCACCAGGAGAAGGCCTTGCTAATAGTTGCGGTACAGCAACATAATGATGTGAAAAACGAACTGGGtgtga                            >  2:1810875/1‑149 (MQ=37)
                                                                                                                           cTGTTGCGGGTGAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACAAACAAGGCCTTCACCTGGATAAGGCCATGCTATTAGTTGCGGTACAGCACCTTAAATCTGTGATATACGAACTGGgttttca                           >  2:1326204/1‑145 (MQ=25)
                                                                                                                             gTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGc                         <  1:3304012/150‑1 (MQ=255)
                                                                                                                               tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCgg                       <  2:5466733/150‑1 (MQ=255)
                                                                                                                               tGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCACTCCCACAGGGAGAGGGAGAAAAAACACAAGGCCTTCTACTGGAGAAGGCCGTGCTATTAGTTGAGGTACAGCACCTTAATGATGTGATATACGAACAGGGTGTGCAGCgg                       >  1:1309752/1‑150 (MQ=38)
                                                                                                                                  gggTTAAGTGCGCGCTGATGCCCTCCCCCCCACCCAAACCCACACGGAGAGGGAGAAAAAAAACAAGGCCGTCACCGGGGGAAAGCCATGCTATTAGTTGCGGTACAGCACCATAATGATGTGATAAACGAAAAGGGTGTGGAGCGGGcc                    >  2:8160/1‑150 (MQ=255)
                                                                                                                                  gggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGcc                    <  1:2996579/150‑1 (MQ=255)
                                                                                                                                   ggTTAAGTGCGCGCTGATGCCCTCACCCCCACCCACACCCACACGGAGAGGGAGAAAACACACAATGCCATAACCAGGAGAAGGCCATGCTATTAATTTAGGTACAGCACCATAATGATGTGATATACGAACAGGGTGTGCAGCGGGCCg                   >  1:857319/1‑150 (MQ=25)
                                                                                                                                      tAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc                <  1:383430/150‑1 (MQ=255)
                                                                                                                                          tGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCt            <  1:1433393/150‑1 (MQ=255)
                                                                                                                                             gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa         <  2:4801835/150‑1 (MQ=255)
                                                                                                                                              cgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAg        <  1:340841/150‑1 (MQ=255)
                                                                                                                                              cgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAg        <  1:3595136/150‑1 (MQ=255)
                                                                                                                                                   aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCACGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg   <  1:4941543/150‑1 (MQ=255)
                                                                                                                                                   aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACCGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg   >  2:2801326/1‑150 (MQ=255)
                                                                                                                                                   aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg   <  1:3541670/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGG  >  CP009273/3952669‑3952966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑