Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,382,369 T→A 7.1% intergenic (+5/‑31) glyX → / → glyY tRNA‑Gly/tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,382,3690TA 7.1% 677.1 / 10.4 256intergenic (+5/‑31)glyX/glyYtRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base T (135/92);  new base A (20/4);  total (159/97)
Fisher's exact test for biased strand distribution p-value = 2.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

GGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTA  >  CP009273/4382220‑4382518
                                                                                                                                                     |                                                                                                                                                      
tggTCGCGATTTCTTGTCTCGGTTCCCTCTCCAAAATTTGATAAGTGCTGCATAGCTCAGATCACCTAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCTAGTCTCGTTTCCCGCTCCAAAAtt                                                                                                                                                        <  2:2680976/149‑1 (MQ=14)
gggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGTAATAGCTCATTGGGTAGAGAAAGACATGGCCAAGGTCGGGGTCGCGAGTTCTAGTCTCGTTTCCCGCGCCAAAAtt                                                                                                                                                        >  2:5122174/1‑150 (MQ=14)
gggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCATTGTCGGGGTCGAGAGTTCGAGTCTCGTTACCCGATCCAAAAtt                                                                                                                                                        >  2:1520696/1‑150 (MQ=14)
   tCGCTATTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAATTGCTGCATAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑a                                                                                                                                                    <  2:5363289/150‑1 (MQ=255)
   tCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCCAAATTTG‑a                                                                                                                                                    >  1:4988073/1‑150 (MQ=255)
   tCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGACTCGTTTCCCGCTCCCAAATATG‑a                                                                                                                                                    >  2:3128354/1‑150 (MQ=255)
          ttCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCt                                                                                                                                             <  1:1401841/150‑1 (MQ=255)
          ttCGAGTCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCt                                                                                                                                             <  2:4632818/150‑1 (MQ=255)
             gAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTT‑AAATAGCTGTa                                                                                                                                          >  2:2451323/1‑150 (MQ=255)
             gAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAATTGCTGTa                                                                                                                                          >  1:3230339/1‑150 (MQ=255)
             gAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTa                                                                                                                                          <  2:373477/150‑1 (MQ=255)
              aGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTaa                                                                                                                                         <  1:5374468/150‑1 (MQ=255)
              aGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTaa                                                                                                                                         >  1:1761609/1‑150 (MQ=255)
               gTCTCGTTTCCCGCTCCAAAATGTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAg                                                                                                                                        <  1:576078/150‑1 (MQ=255)
                   cGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCAAAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACATTGCCAAGGTCGGGGTCGCGAGTTCGAGTATCGTTTCCCGCTCCAAAATATT‑AAATTGATGTAATGcac                                                                                                                                    >  2:4646776/1‑150 (MQ=14)
                    gTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGAGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGAAAGGcaca                                                                                                                                   >  1:3314566/1‑150 (MQ=255)
                       tCCCGCTCAAAAATTTGAAAAGTGCTGCAAAGCACAGACCAACCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGac                                                                                                                                <  2:2033410/150‑1 (MQ=255)
                        cccGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAATAGATGAAAGGAACAGacc                                                                                                                               >  2:79560/1‑150 (MQ=17)
                        cccGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGacc                                                                                                                               <  1:1147266/150‑1 (MQ=255)
                         ccGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGacca                                                                                                                              <  1:3622473/150‑1 (MQ=255)
                          cGCTCCAAAATTTGAAAAGTTCTGCAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGaccac                                                                                                                             <  2:2563641/150‑1 (MQ=255)
                          cGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑ATAGTGATTTAAGTAACAGaccac                                                                                                                             >  2:2912346/1‑150 (MQ=17)
                            cTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCAccc                                                                                                                           >  2:2096780/1‑150 (MQ=255)
                                aaaaTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTG‑AACTTGCTGTAAGGCACAGACCACCCCAga                                                                                                                       >  2:3437244/1‑149 (MQ=255)
                                  aaTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATG‑AAAGTGCTGAAAGGCACAGACCACCCAACCgg                                                                                                                     >  2:3954659/1‑150 (MQ=255)
                                   aTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGACCAGACCACCCAAGCggg                                                                                                                    >  2:4285982/1‑150 (MQ=255)
                                     ttGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGAGCTTTAATGCACAGACCACCCAAGCGGGaa                                                                                                                  >  1:4730824/1‑150 (MQ=255)
                                        aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAg                                                                                                               >  1:4775944/1‑150 (MQ=255)
                                         aaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGGG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGc                                                                                                              >  2:4943662/1‑150 (MQ=255)
                                             tgctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAg                                                                                                          <  2:5136244/150‑1 (MQ=255)
                                               ctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGtt                                                                                                        >  1:770063/1‑150 (MQ=255)
                                                tgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAATCGGGAAAAGCTCAGTTg                                                                                                       >  2:1000607/1‑150 (MQ=255)
                                                 gcAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg                                                                                                      <  2:3935817/150‑1 (MQ=255)
                                                 gcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg                                                                                                      <  1:4930061/150‑1 (MQ=255)
                                                  cAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGAACAGACCACCCAAGTGGGACTAGCGCCGTTGGt                                                                                                     >  1:391298/1‑150 (MQ=17)
                                                      tcacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTagag                                                                                                 <  1:1492060/149‑1 (MQ=255)
                                                       cacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGc                                                                                                >  1:4669715/1‑150 (MQ=255)
                                                          aGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACg                                                                                             >  1:2471193/1‑150 (MQ=255)
                                                          aGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGAAAGGCACAGAGCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACg                                                                                             >  2:1614678/1‑150 (MQ=255)
                                                            accaagcAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGGTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGAc                                                                                           <  2:3564415/144‑1 (MQ=255)
                                                                 ccAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAATCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGc                                                                                      >  1:1232308/1‑150 (MQ=255)
                                                                  cacgCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGcc                                                                                     <  1:4195559/147‑1 (MQ=37)
                                                                  cAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTgac                                                                                     >  2:1437506/1‑148 (MQ=255)
                                                                   aaGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAATGAACAGACCACCCAAGCGGGAATAGATCAGTAGTTAGAGCACGACCTTGCCa                                                                                    >  2:703975/1‑150 (MQ=11)
                                                                   aaGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                    >  1:1294162/1‑150 (MQ=40)
                                                                   aaGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTATCCCGCTCCAAAATTTG‑AAATTGCTGTAAGTCACAGACCACCCAAGCGGGAAAAGCTCAGTTGGTAGATCACGACCATCCCa                                                                                    >  1:3005261/1‑150 (MQ=14)
                                                                      cGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAgg                                                                                 >  2:4722211/1‑150 (MQ=38)
                                                                       gggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACATTGCCAATGt                                                                                >  1:532720/1‑150 (MQ=255)
                                                                          aagtgCTCTGTTGGTAGACCACGACCTTGCCAAGGTGGGGGTCGGGTGTTCGAGTCTCGTTTCCCGCTCCAAAATTGG‑AAAGTGCTGTAATGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                             <  2:4021511/146‑1 (MQ=11)
                                                                          aaTCGCTCAGTTTGTAGAGCCCGACCTTCCCAAGGTCGGGGTCCCGTGTCCGTGTTTCGCTTCCCGCCCCAATAATGT‑TAAGTTCTGTATCGCACAGAACTCCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                             <  2:3026876/150‑1 (MQ=2)
                                                                          aaTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTTCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGTTCgg                                                                             >  2:3109631/1‑150 (MQ=255)
                                                                          aaTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCAAGACCTTGCCAAGGTCgg                                                                             >  2:4829362/1‑150 (MQ=38)
                                                                           aTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTACCGCTCCAAAATTTG‑AAAGTGCTGTAATGCACAGACCACCCCAGCGGGAATAGCTCAGTGGGTAGTGCAAGACCATGCCAAGGTCggg                                                                            >  2:3791490/1‑150 (MQ=11)
                                                                             aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGAGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGt                                                                          >  1:1443646/1‑150 (MQ=38)
                                                                              gCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                         >  1:2121678/1‑150 (MQ=38)
                                                                              gCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                         >  1:3373182/1‑150 (MQ=38)
                                                                               cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAATGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcg                                                                        >  2:1880602/1‑150 (MQ=25)
                                                                                     tGGTTGAGCACTCCCTTGCCAATGTGGTGGTCTCTAGTTCGAGTCCCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTATGGCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                                                  <  2:1954765/150‑1 (MQ=11)
                                                                                        tAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATG‑AAATTGCTGTAAGGCACAGACCACCCAAGAGGGAAAAGCTCCGTTGTTCGAGACCGACCTTGACAAGTTCCGGGTCGCGattacta                                                               >  2:3109018/1‑144 (MQ=11)
                                                                                         agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCATGCCAAGGTCGGGGTCGCGAGTTCGAg                                                              >  2:4999707/1‑150 (MQ=38)
                                                                                             cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc                                                          >  1:4943917/1‑150 (MQ=38)
                                                                                              aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCg                                                         >  1:3861342/1‑150 (MQ=38)
                                                                                              aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGAGGGAATAGCTAAGTTGGTATAGCACGACCAAGCCCAGGTCGGGGTCGCGAGTTCGAGTCTCg                                                         >  2:1687597/1‑150 (MQ=11)
                                                                                                 aCCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGttt                                                      <  1:4108628/150‑1 (MQ=38)
                                                                                                       ccAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCt                                                >  1:4432748/1‑150 (MQ=38)
                                                                                                          aGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCa                                             >  2:538521/1‑150 (MQ=38)
                                                                                                           ggTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaa                                            >  1:3792070/1‑150 (MQ=38)
                                                                                                            gTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCt                                                >  1:858816/1‑145 (MQ=38)
                                                                                                            gTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCt                                                <  2:858816/145‑1 (MQ=38)
                                                                                                             tCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGGGGGAATAGCTCAGTTGGTAGAGAACGACCTTGCCAAGGTCGGGGTCTCGAGTTCGAGTCTCGTTTCCCGCTCCAAAt                                          >  2:5214010/1‑150 (MQ=255)
                                                                                                             tCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAt                                          >  1:2304629/1‑150 (MQ=39)
                                                                                                                 ggactccaGTTCTAGTCTCGTTTCCCGCACCAAAATTTG‑AAAGTGCTGAAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAttctt                                      <  2:1195801/143‑1 (MQ=14)
                                                                                                                 ggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAttctt                                      <  2:1761609/150‑1 (MQ=255)
                                                                                                                  gTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAttcttc                                     >  1:1616196/1‑150 (MQ=255)
                                                                                                                   tCGCTAGTACGAGTCGCGTTTCCCGCTCCAAATTTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATtcttct                                    <  1:3662870/150‑1 (MQ=255)
                                                                                                                   tCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATtcttct                                    >  1:1113955/1‑150 (MQ=255)
                                                                                                                     gcgAGTTCGAGTCTCTTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctct                                  <  1:5078685/150‑1 (MQ=255)
                                                                                                                      cttttccGAGTCCCGTTACCCGCAACAAAATATT‑AAAGTGCTGTATGGCAAATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctctc                                 <  1:370694/144‑1 (MQ=14)
                                                                                                                           tCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATaa                            >  1:2100807/1‑150 (MQ=255)
                                                                                                                             ttgTCGCGTTTCGCGGTCGAAATGTGT‑AAAGTGCTTTATCTCACAGACGAAGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATaaaa                          <  2:3193064/148‑1 (MQ=14)
                                                                                                                              aGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGATGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTGTCCCGCTCCAAATTCTTCTCTCAATaaaag                         >  2:5267963/1‑149 (MQ=255)
                                                                                                                               gTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTAGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCATATGCTTCTCTCTATAAAata                        >  2:4164846/1‑150 (MQ=255)
                                                                                                                                tctcGTTTCCCGCTCCAAACTTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAatat                       <  1:1956293/150‑1 (MQ=255)
                                                                                                                                 ctcGTTTCCCGCTCCAAAAGTTG‑AAAGTGCTGTAAGGCTCAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATc                      <  1:4285982/150‑1 (MQ=255)
                                                                                                                                   ggTTTCCGGATCCAAATTTTT‑AAAGTGCTGAAATGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCca                    <  2:1272582/149‑1 (MQ=17)
                                                                                                                                     tttCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcaca                  >  1:1884342/1‑150 (MQ=255)
                                                                                                                                      ttCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAg                 >  1:2811580/1‑150 (MQ=255)
                                                                                                                                        cccGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCCAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCg               >  2:4736858/1‑150 (MQ=255)
                                                                                                                                        cccGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCg               >  2:3063741/1‑150 (MQ=255)
                                                                                                                                        cccGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCg               <  2:2185939/150‑1 (MQ=255)
                                                                                                                                        cccGCTCCAAAATTTG‑AAAGTGCTGAAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCg               <  2:4847401/150‑1 (MQ=255)
                                                                                                                                            cTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcgc           >  1:4573240/1‑150 (MQ=255)
                                                                                                                                             tCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcgcg          >  2:968688/1‑150 (MQ=255)
                                                                                                                                             tCCAAAATATT‑AAAGTTCTGTAAGGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcgcg          <  1:2934261/150‑1 (MQ=255)
                                                                                                                                              ccAAAATTTGAAAAGTGCTTCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcgcg          <  2:4609112/150‑1 (MQ=14)
                                                                                                                                                aaaaTTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATg       <  1:199590/150‑1 (MQ=255)
                                                                                                                                                aaaaTTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGATCAGTTGGTAGAGCACGACCTTTCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTATCTCAATAAAATATCAACAGCTACGCGATg       >  2:3150119/1‑150 (MQ=18)
                                                                                                                                                  aaTTGG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCg     <  1:4932991/150‑1 (MQ=255)
                                                                                                                                                    tttG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGtt   <  1:4646659/150‑1 (MQ=255)
                                                                                                                                                     ttG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTa  <  1:703975/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTA  >  CP009273/4382220‑4382518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑