BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	317,203	G→A	100%	intergenic (+411/‑116)	rclR → / → ykgE	reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	317,203	0	G	A	99.2%	849.0 / ‑2.4	262	intergenic (+411/‑116)	rclR/ykgE	reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (119/140); total (121/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 263 total aligned reads displayed.

GTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTA  >  CP009273/317056‑317346

       ggATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtg                                                                                                                                          <  2:271434/148‑1 (MQ=255)

       ggATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtg                                                                                                                                          <  2:2716750/148‑1 (MQ=255)

            tAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATg                                                                                                                                     <  1:2803253/148‑1 (MQ=255)

            tAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATg                                                                                                                                     <  1:2572901/148‑1 (MQ=255)

                cATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGc                                                                                                                                 >  2:2307408/1‑148 (MQ=255)

                cATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGc                                                                                                                                 <  1:3019116/148‑1 (MQ=255)

                   ttttAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAg                                                                                                                              <  2:615646/148‑1 (MQ=255)

                   ttttAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAg                                                                                                                              >  1:2931265/1‑148 (MQ=255)

                   ttttAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAg                                                                                                                              >  2:3016075/1‑148 (MQ=255)

                         aTGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAtt                                                                                                                        >  1:1327448/1‑148 (MQ=255)

                           ggATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaa                                                                                                                      >  2:2693741/1‑148 (MQ=255)

                           ggATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaa                                                                                                                      >  2:2848740/1‑148 (MQ=255)

                            gATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaa                                                                                                                     <  1:446537/148‑1 (MQ=255)

                            gATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaa                                                                                                                     >  1:633542/1‑148 (MQ=255)

                            gATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaa                                                                                                                     >  1:2063730/1‑148 (MQ=255)

                            gATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaa                                                                                                                     >  1:1752987/1‑148 (MQ=255)

                               ttCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATACAGGGTGGTATGTTGCTAGCCAATTaaaaaa                                                                                                                  >  2:2597435/1‑148 (MQ=255)

                                      gCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGGTTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGcc                                                                                                           >  1:711975/1‑148 (MQ=255)

                                      gCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGcc                                                                                                           >  1:920097/1‑148 (MQ=255)

                                         aaaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                                                        >  2:398801/1‑148 (MQ=255)

                                         aaaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                                                        <  1:1483728/148‑1 (MQ=255)

                                         aaaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                                                        >  2:2167140/1‑148 (MQ=255)

                                          aaaaTGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCatat                                                                                                       <  1:3491537/148‑1 (MQ=255)

                                          aaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCatat                                                                                                       <  2:1457872/148‑1 (MQ=255)

                                           aaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtatt                                                                                                      >  1:2322765/1‑148 (MQ=255)

                                             aTGAACGTCGATTTCCCCCTAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtattta                                                                                                    >  2:1147934/1‑148 (MQ=255)

                                             aTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtattta                                                                                                    <  2:2772648/148‑1 (MQ=255)

                                              ttaacgtctATTTCCCCATAAAATGTGAGCTATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCTTTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCATTTAAAAAAGAACGCCAtatttat                                                                                                   <  1:1284103/144‑1 (MQ=255)

                                              tGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtatttat                                                                                                   >  1:2244685/1‑148 (MQ=255)

                                              tGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtatttat                                                                                                   >  1:24385/1‑148 (MQ=255)

                                              tGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtatttat                                                                                                   >  1:1804175/1‑148 (MQ=255)

                                               caacgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAACAACGCCAtatttatt                                                                                                  <  1:2509882/145‑1 (MQ=255)

                                                aacgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtg                                                                                                 <  1:2848816/146‑1 (MQ=255)

                                                aacgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtg                                                                                                 <  2:1767173/146‑1 (MQ=255)

                                                aacgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtg                                                                                                 <  2:1168517/146‑1 (MQ=255)

                                                 acgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtga                                                                                                >  2:870981/2‑148 (MQ=255)

                                                 acgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTAGGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtga                                                                                                >  2:871243/2‑148 (MQ=255)

                                                    tcgATTTCCCCATAAAATGTGAGCGATGCCGAAATAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtgatga                                                                                             <  2:3143863/148‑1 (MQ=255)

                                                    tcgATGTCCCCATAAAATTTGAGCGATGCCGAAACAAATAAATTTATTTATTGCATTTGACTTTTGGATGAAATATTATCATTTGTCATACAAATAATGGTTGGTATGTTGCTAGCCAATTAAAAAACAACGCCATATTTATtgatga                                                                                             <  1:2727737/148‑1 (MQ=255)

                                                     ggATTTCCCCATAAAATGTGAGCTATGCCGAAAGAAATAAAATTAGTTTTTGCATTTGACGTTTGGATGAAATATTATCATTTTTCATAAAAATAATGGGTGGTATGTTGCTAGCAAATTAAAAAAGAACGCCATATTTATTgatgat                                                                                            <  1:401708/147‑1 (MQ=255)

                                                        tttCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTTTTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAATTACAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattga                                                                                         <  1:2479631/148‑1 (MQ=255)

                                                            cccATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATccc                                                                                     <  2:1121512/148‑1 (MQ=255)

                                                              cATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATccccc                                                                                   >  1:2882485/1‑148 (MQ=255)

                                                                tAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCgg                                                                                 <  2:1335110/148‑1 (MQ=255)

                                                                 aaaaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGt                                                                                >  1:1220369/1‑148 (MQ=255)

                                                                  aaaTGTGAGCGATGCCGAAAGAAATAAAATTCGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg                                                                               <  1:2414796/148‑1 (MQ=255)

                                                                          gCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGaa                                                                       <  2:777882/148‑1 (MQ=255)

                                                                            gATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAACGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAAc                                                                     <  1:39260/148‑1 (MQ=255)

                                                                             aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACa                                                                    <  2:2063730/148‑1 (MQ=255)

                                                                                  gaaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCggg                                                               <  2:2883382/148‑1 (MQ=255)

                                                                                  gaaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCggg                                                               >  1:738002/1‑148 (MQ=255)

                                                                                     agaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCACGGTGGGGGGGAAACATCGGGCAt                                                            >  2:1436248/1‑148 (MQ=255)

                                                                                        aaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATaaa                                                         <  2:210635/148‑1 (MQ=255)

                                                                                         aTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAAt                                                        <  1:1097827/148‑1 (MQ=255)

                                                                                          tAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATg                                                       <  2:920379/148‑1 (MQ=255)

                                                                                          tAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATg                                                       <  1:3145393/148‑1 (MQ=255)

                                                                                            aaaTTAGTTATTGATTTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAATGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATggg                                                     <  1:1544580/148‑1 (MQ=255)

                                                                                             aaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGc                                                    <  1:1696956/148‑1 (MQ=255)

                                                                                                tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                 >  1:94706/1‑148 (MQ=255)

                                                                                                tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                 <  2:705437/148‑1 (MQ=255)

                                                                                                tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                 >  2:3477642/1‑148 (MQ=255)

                                                                                                  gTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGaa                                               >  1:821243/1‑148 (MQ=255)

                                                                                                   ttATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                              <  1:853100/148‑1 (MQ=255)

                                                                                                   ttATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                              >  1:1146379/1‑148 (MQ=255)

                                                                                                   ttATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                              <  1:1138022/148‑1 (MQ=255)

                                                                                                   ttATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGGGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                              <  2:2751179/148‑1 (MQ=255)

                                                                                                    tATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGt                                             <  2:236305/148‑1 (MQ=255)

                                                                                                     aTTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTa                                            <  1:3363125/148‑1 (MQ=255)

                                                                                                      ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa                                           <  2:2924912/148‑1 (MQ=255)

                                                                                                       tGCATTTGACGTTTGTATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt                                          <  1:1800450/148‑1 (MQ=255)

                                                                                                          ctttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGa                                       <  2:1016267/147‑1 (MQ=255)

                                                                                                          aTTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGa                                       <  2:3338023/148‑1 (MQ=255)

                                                                                                            ttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGt                                     >  2:1532512/1‑148 (MQ=255)

                                                                                                              gACGTTTGGATGAAAGATTATCTTTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAt                                   <  2:1832303/148‑1 (MQ=255)

                                                                                                              gACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg                                                      >  2:852174/1‑129 (MQ=255)

                                                                                                              gACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg                                                      <  1:852174/129‑1 (MQ=255)

                                                                                                              gACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAt                                   <  1:1037417/148‑1 (MQ=255)

                                                                                                               aCGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAtt                                  <  1:2122701/148‑1 (MQ=255)

                                                                                                                cGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTa                                 >  2:2411436/1‑148 (MQ=255)

                                                                                                                   ttGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGtt                              >  2:1876990/1‑148 (MQ=255)

                                                                                                                    tGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtg                             <  2:1688942/148‑1 (MQ=255)

                                                                                                                    tGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtg                             <  1:585903/148‑1 (MQ=255)

                                                                                                                    tGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtg                             <  2:1689974/148‑1 (MQ=255)

                                                                                                                     ggATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTCTTAGTtgt                            >  2:2906786/1‑148 (MQ=255)

                                                                                                                       aTGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGa                          >  2:3452879/1‑148 (MQ=255)

                                                                                                                            aGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg                                        >  1:2567756/1‑129 (MQ=255)

                                                                                                                              aTTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCaa                   <  2:415311/148‑1 (MQ=255)

                                                                                                                              aTTATAATTTGTCATACATATAATGGTTGTTATGTTTCTAGGCATTTAAAAAAGAACTCTAGATTTATTGATGATTGATCCCCCGGTGGTGGTGTAACATCGGGCATATATGGTCATGAAGTATTGGAGTATTAGTTGTGAATGTcta                   <  2:1815676/148‑3 (MQ=255)

                                                                                                                                tATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                 >  1:1972138/1‑148 (MQ=255)

                                                                                                                                tATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                 >  1:2788026/1‑148 (MQ=255)

                                                                                                                                tATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                 >  1:1251521/1‑148 (MQ=255)

                                                                                                                                 aTCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAttt                <  2:1602884/148‑1 (MQ=255)

                                                                                                                                   cATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCt              <  2:2244685/148‑1 (MQ=255)

                                                                                                                                   cATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCt              >  2:1626357/1‑148 (MQ=255)

                                                                                                                                    aTTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCtt             <  1:3072818/148‑1 (MQ=255)

                                                                                                                                          cATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc       <  2:1424553/148‑1 (MQ=255)

                                                                                                                                            tACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt     <  2:3012005/148‑1 (MQ=255)

                                                                                                                                             aCAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTg    <  2:2864328/148‑1 (MQ=255)

                                                                                                                                              cAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGt   >  2:2440008/1‑148 (MQ=255)

                                                                                                                                               aaaTAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTa  <  1:1613013/148‑1 (MQ=255)

GTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTA  >  CP009273/317056‑317346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`