Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,230,913 A→T 100% W424R (TGG→AGG)  xylE ← D‑xylose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,230,9130AT100.0% 725.5 / NA 217W424R (TGG→AGG) xylED‑xylose transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (105/112);  total (105/112)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

TCAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCA  >  CP009273/4230768‑4231056
                                                                                                                                                 |                                                                                                                                               
tcAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTgg                                                                                                                                               >  1:2120913/1‑148 (MQ=255)
tcAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTgg                                                                                                                                               >  2:2684893/1‑148 (MQ=255)
 cAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGga                                                                                                                                              >  1:2316979/1‑148 (MQ=255)
   aGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGgaga                                                                                                                                            <  1:995204/148‑1 (MQ=255)
    gggTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGAc                                                                                                                                           >  2:1197929/1‑148 (MQ=255)
       ttttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGaa                                                                                                                                        <  1:1253168/148‑1 (MQ=255)
       ttttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGaa                                                                                                                                        >  2:2974216/1‑148 (MQ=255)
        tttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAag                                                                                                                                       <  1:2441304/148‑1 (MQ=255)
          tACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagta                                                                                                                                     >  1:814590/1‑148 (MQ=255)
           aCCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACCACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagtag                                                                                                                                    <  2:1514136/148‑1 (MQ=255)
           aCCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagtag                                                                                                                                    >  2:3019811/1‑148 (MQ=255)
                tGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgc                                                                                                                               <  2:461714/148‑1 (MQ=255)
                  gTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgcca                                                                                                                             >  2:2994094/1‑148 (MQ=255)
                       cGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCAc                                                                                                                        <  2:1832082/148‑1 (MQ=255)
                         ggACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTg                                                                                                                      <  1:3084856/148‑1 (MQ=255)
                               aTTTCCACATAAACAGTGCTGCCAGCACGCCCATACAACCGTAAATCCTGTAGGAGAAACCGTTGTGGAAATGGGCCACGAGCCAGGAGTTTTTGTCCATCATCGGGATGGTCCTGGAGTCGAAGTAGTTCGCCAGCCACTGGgccgc                                                                                                                <  2:3488313/148‑1 (MQ=255)
                                tttCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGgccgcc                                                                                                               >  2:1336072/1‑148 (MQ=255)
                                 ttCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCa                                                                                                              >  1:405981/1‑148 (MQ=255)
                                     acaTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACcgc                                                                                                          >  1:1918206/1‑148 (MQ=255)
                                     acaTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACcgc                                                                                                          >  1:1935079/1‑148 (MQ=255)
                                      caTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACcgcg                                                                                                         <  2:1998308/148‑1 (MQ=255)
                                       aTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGa                                                                                                        <  1:2056522/148‑1 (MQ=255)
                                        tAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAt                                                                                                       >  2:2837008/1‑148 (MQ=255)
                                        tAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAt                                                                                                       <  1:1093189/148‑1 (MQ=255)
                                         aaaCAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAtt                                                                                                      >  1:2130881/1‑148 (MQ=255)
                                         aaaCAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAtt                                                                                                      >  1:2868683/1‑148 (MQ=255)
                                          aaCAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTg                                                                                                     >  1:3352099/1‑148 (MQ=255)
                                             aGTGCTGCCAGCACGCCCATACCACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCa                                                                                                  <  2:1796398/148‑1 (MQ=255)
                                              gTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAg                                                                                                 >  2:55693/1‑148 (MQ=255)
                                               tgctgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAgc                                                                                                >  2:543492/1‑148 (MQ=255)
                                                gctgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAgcg                                                                                               <  2:2371355/148‑1 (MQ=255)
                                                gctgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGGTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAgcg                                                                                               <  1:550258/148‑1 (MQ=255)
                                                  tgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCt                                                                                             <  1:304731/148‑1 (MQ=255)
                                                     cAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTa                                                                                          <  1:1197929/148‑1 (MQ=255)
                                                        aaCGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCa                                                                                       <  2:256279/148‑1 (MQ=255)
                                                          cGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACg                                                                                     <  2:2357293/148‑1 (MQ=255)
                                                             ccATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAAt                                                                                  >  2:3105203/1‑148 (MQ=255)
                                                              cATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATa                                                                                 <  2:2314699/148‑1 (MQ=255)
                                                               aTACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAg                                                                                >  1:869143/1‑148 (MQ=255)
                                                                tACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGc                                                                               <  1:1527694/148‑1 (MQ=255)
                                                                tACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGc                                                                               <  1:3268537/148‑1 (MQ=255)
                                                                  cAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCAt                                                                             >  2:777622/1‑148 (MQ=255)
                                                                     ccGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCg                                                                          <  2:1486207/148‑1 (MQ=255)
                                                                     ccGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCg                                                                          >  2:1233131/1‑148 (MQ=255)
                                                                       gTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCggg                                                                        <  1:2294040/148‑1 (MQ=255)
                                                                        tAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGa                                                                       >  2:1811127/1‑148 (MQ=255)
                                                                        tAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGa                                                                       <  1:1103954/148‑1 (MQ=255)
                                                                              cAGGAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGAttt                                                                 <  2:1633208/148‑1 (MQ=255)
                                                                                  aGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGa                                                             <  1:3075604/148‑1 (MQ=255)
                                                                                   ggAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAc                                                            >  1:2689476/1‑148 (MQ=255)
                                                                                   ggAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAc                                                            >  1:90529/1‑148 (MQ=255)
                                                                                   ggAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAc                                                            >  1:91366/1‑148 (MQ=255)
                                                                                   ggAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACAACGAATAGCATTCGGGAAGATTTCCGAc                                                            >  2:290487/1‑148 (MQ=255)
                                                                                     agaAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCCGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcag                                                          >  2:2163214/1‑148 (MQ=255)
                                                                                       aaaCCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcagca                                                        <  1:1638133/148‑1 (MQ=255)
                                                                                          ccGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGtc                                                     >  2:3409136/1‑147 (MQ=255)
                                                                                          ccGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTa                                                     <  1:703506/148‑1 (MQ=255)
                                                                                           cGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTAc                                                    >  2:2334635/1‑148 (MQ=255)
                                                                                            gTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTAcc                                                   >  2:1159780/1‑148 (MQ=255)
                                                                                            gTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTAcc                                                   <  1:3007589/148‑1 (MQ=255)
                                                                                            gTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTAcc                                                   <  1:1950075/148‑1 (MQ=255)
                                                                                              tgtgGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCa                                                 >  1:2523650/1‑148 (MQ=255)
                                                                                                tgGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGc                                               <  2:1043715/148‑1 (MQ=255)
                                                                                                 ggAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCa                                              >  1:1528334/1‑148 (MQ=255)
                                                                                                  gAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCAt                                             >  1:3460072/1‑148 (MQ=255)
                                                                                                  gAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCAt                                             <  1:3019811/148‑1 (MQ=255)
                                                                                                      tGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCg                                         >  2:335489/1‑148 (MQ=255)
                                                                                                      tGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCg                                         >  2:3158921/1‑148 (MQ=255)
                                                                                                      tGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCg                                         >  1:2647701/1‑148 (MQ=255)
                                                                                                      tGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCg                                         >  1:541157/1‑148 (MQ=255)
                                                                                                           caccaGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGAccc                                    <  1:1026898/148‑1 (MQ=255)
                                                                                                             ccagccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCa                                  <  2:746222/148‑1 (MQ=255)
                                                                                                             ccagccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCa                                  <  2:747052/148‑1 (MQ=255)
                                                                                                             ccagccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCa                                  <  2:2559205/148‑1 (MQ=255)
                                                                                                               agccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAgg                                <  2:1727928/148‑1 (MQ=255)
                                                                                                                 ccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGAc                              >  1:3165085/1‑148 (MQ=255)
                                                                                                                 ccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCAAACCGGACCCCAGGAc                              >  2:2846010/1‑148 (MQ=255)
                                                                                                                   agGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACAt                            >  1:1677775/1‑148 (MQ=255)
                                                                                                                     gAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATgg                          <  1:2719245/148‑1 (MQ=255)
                                                                                                                      aGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGc                         >  1:943272/1‑148 (MQ=255)
                                                                                                                        tttttGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCaa                       >  1:1731280/1‑148 (MQ=255)
                                                                                                                         ttttGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCaaa                      <  1:1336072/148‑1 (MQ=255)
                                                                                                                         ttttGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCaaa                      <  1:1447659/148‑1 (MQ=255)
                                                                                                                          tttGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAg                     <  2:1553954/148‑1 (MQ=255)
                                                                                                                            tGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggc                   <  1:2974216/148‑1 (MQ=255)
                                                                                                                              tCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcgg                 <  2:195404/148‑1 (MQ=255)
                                                                                                                               ccATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcggc                >  2:2247446/1‑148 (MQ=255)
                                                                                                                               ccATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcggc                >  2:582112/1‑148 (MQ=255)
                                                                                                                                  tcatcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAAc             <  1:2003292/148‑1 (MQ=255)
                                                                                                                                   catcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACa            <  2:3352099/148‑1 (MQ=255)
                                                                                                                                     tcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATa          <  1:3513341/148‑1 (MQ=255)
                                                                                                                                      cGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAg         >  1:1196499/1‑148 (MQ=255)
                                                                                                                                      cGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAg         >  1:2736378/1‑148 (MQ=255)
                                                                                                                                        ggAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGaa       >  1:2992230/1‑148 (MQ=255)
                                                                                                                                        ggAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGaa       >  1:1002564/1‑148 (MQ=255)
                                                                                                                                            ggTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGc   <  1:1419853/148‑1 (MQ=255)
                                                                                                                                            ggTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGc   >  1:634013/1‑148 (MQ=255)
                                                                                                                                            ggTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGc   <  1:3166254/148‑1 (MQ=255)
                                                                                                                                             gTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCa  <  2:271904/148‑1 (MQ=255)
                                                                                                                                             gTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCa  >  2:1935760/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TCAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCA  >  CP009273/4230768‑4231056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑