Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,235,869 G→T 100% A160E (GCG→GAG)  malE ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,235,8690GT100.0% 823.4 / ‑6.7 256A160E (GCG→GAG) malEmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (126/129);  total (127/129)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 256 total aligned reads displayed.

CCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCT  >  CP009273/4235726‑4236013
                                                                                                                                               |                                                                                                                                                
 cACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCggg                                                                                                                                             <  2:2278178/148‑1 (MQ=255)
  aCGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGa                                                                                                                                            <  2:148930/148‑1 (MQ=255)
   cGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGAt                                                                                                                                           <  2:252486/148‑1 (MQ=255)
          aaTGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTcc                                                                                                                                    <  1:2437986/148‑1 (MQ=255)
          aaTGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTcc                                                                                                                                    >  2:1438847/1‑148 (MQ=255)
              tCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAgg                                                                                                                                >  2:1021008/1‑148 (MQ=255)
               cGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGt                                                                                                                               <  2:2067581/148‑1 (MQ=255)
                 tACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGttt                                                                                                                             <  2:2960204/148‑1 (MQ=255)
                  aCTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGtttt                                                                                                                            <  1:3352102/148‑1 (MQ=255)
                  aCTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATACAGCTCCGGGATCTCTTACCAGGtttt                                                                                                                            >  1:1929375/1‑148 (MQ=255)
                   cTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGttttt                                                                                                                           >  2:124660/1‑148 (MQ=255)
                   cTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGGTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGttttt                                                                                                                           <  2:39590/148‑1 (MQ=255)
                    ttGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTg                                                                                                                          <  2:270894/148‑1 (MQ=255)
                         gTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCggg                                                                                                                     >  1:3111605/1‑148 (MQ=255)
                         gTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCggg                                                                                                                     <  1:359267/148‑1 (MQ=255)
                          ttttCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCCGGTTTTTGGCgggg                                                                                                                    >  2:690336/1‑147 (MQ=255)
                          ttttCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGt                                                                                                                    <  2:2196450/148‑1 (MQ=255)
                          ttttCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGt                                                                                                                    <  2:3375023/148‑1 (MQ=255)
                            ttCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTc                                                                                                                  <  1:2098530/148‑1 (MQ=255)
                               aTACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGgc                                                                                                               >  1:2215947/1‑148 (MQ=255)
                                 aCTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGgcag                                                                                                             <  2:409871/148‑1 (MQ=255)
                                  cTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGgcagc                                                                                                            >  2:2141895/1‑148 (MQ=255)
                                  cTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGgcagc                                                                                                            >  2:1224691/1‑148 (MQ=255)
                                   ttGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGgcagca                                                                                                           >  1:283056/1‑148 (MQ=255)
                                   ttGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGgcagca                                                                                                           <  2:3199835/148‑1 (MQ=255)
                                       aCGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATc                                                                                                       <  1:195153/148‑1 (MQ=255)
                                        cGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCt                                                                                                      >  2:1273070/1‑148 (MQ=255)
                                        cGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCGTTGAGTTCTTTATACAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGGTCGGCAGCAGATCt                                                                                                      >  1:2378298/1‑148 (MQ=255)
                                             aaCCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCtttttt                                                                                                 >  2:2467131/1‑148 (MQ=255)
                                             aaCCCCCGTCAGCAGCAATCAGCGACCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCtttttt                                                                                                 >  2:2803507/1‑148 (MQ=255)
                                             aaCCCCCGTCAGCAGCAATCAGCGACCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCtttttt                                                                                                 >  2:1343769/1‑148 (MQ=255)
                                              aCCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTta                                                                                                <  2:3338812/148‑1 (MQ=255)
                                               cccccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTtat                                                                                               <  2:2897120/148‑1 (MQ=255)
                                                 cccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATaa                                                                                             >  2:1930938/1‑148 (MQ=255)
                                                 cccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATaa                                                                                             >  2:3425792/1‑148 (MQ=255)
                                                  ccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATaaa                                                                                            <  2:2169210/148‑1 (MQ=255)
                                                    gTCAGCTGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATc                                                                                          >  2:3316557/1‑148 (MQ=255)
                                                    gTCAGCTGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATc                                                                                          >  2:3023439/1‑148 (MQ=255)
                                                    gTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATc                                                                                          <  1:627295/148‑1 (MQ=255)
                                                     tCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCa                                                                                         >  2:188301/1‑148 (MQ=255)
                                                       agcagcAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGc                                                                                       <  1:275315/148‑1 (MQ=255)
                                                        gcagcaATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCg                                                                                      >  2:2743684/1‑148 (MQ=255)
                                                        gcagcaATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCg                                                                                      <  1:285863/148‑1 (MQ=255)
                                                        gcagcaATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCg                                                                                      >  2:3501462/1‑148 (MQ=255)
                                                          agcaATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGAt                                                                                    <  2:708604/148‑1 (MQ=255)
                                                            caATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATaa                                                                                  <  1:457909/148‑1 (MQ=255)
                                                            caATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATaa                                                                                  <  2:2575420/148‑1 (MQ=255)
                                                              aTCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATaaag                                                                                >  1:3019908/1‑146 (MQ=255)
                                                              aTCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACg                                                                                <  2:2822426/148‑1 (MQ=255)
                                                               tCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGc                                                                               >  1:1832738/1‑148 (MQ=255)
                                                                cAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCt                                                                              >  2:1414119/1‑148 (MQ=255)
                                                                cAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTAGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCt                                                                              <  1:1750090/148‑1 (MQ=255)
                                                                   cGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCa                                                                           >  2:2416529/1‑148 (MQ=255)
                                                                     gCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAAc                                                                         <  1:205454/148‑1 (MQ=255)
                                                                        aGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGc                                                                      >  2:1184670/1‑148 (MQ=255)
                                                                        aGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCGTTATCCAGCTCCGGGATCTCTTCCCACGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCATCGATAAACCTTCAACAGc                                                                      >  1:2139385/1‑148 (MQ=255)
                                                                          gTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGa                                                                    <  1:119671/148‑1 (MQ=255)
                                                                          gTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGa                                                                    <  2:2758625/148‑1 (MQ=255)
                                                                          gTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGa                                                                    >  2:1146393/1‑148 (MQ=255)
                                                                           tGAACTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGAt                                                                   >  1:2544648/1‑148 (MQ=255)
                                                                            gAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATc                                                                  <  2:825459/148‑1 (MQ=255)
                                                                            gAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATc                                                                  <  1:816390/148‑1 (MQ=255)
                                                                                     ttCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCa                                                         <  1:2693776/148‑1 (MQ=255)
                                                                                        ttGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATc                                                      <  1:2967001/148‑1 (MQ=255)
                                                                                         tGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCa                                                     <  1:1092370/148‑1 (MQ=255)
                                                                                                 gAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCg                                             <  1:350569/148‑1 (MQ=255)
                                                                                                  aaCATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGt                                            >  1:1276505/1‑148 (MQ=255)
                                                                                                  aaCATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGt                                            >  1:1726912/1‑148 (MQ=255)
                                                                                                      tCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTa                                        <  1:1349293/148‑1 (MQ=255)
                                                                                                       cAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTaa                                       <  1:2843235/148‑1 (MQ=255)
                                                                                                       cAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTaa                                       <  1:1507799/148‑1 (MQ=255)
                                                                                                       cAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTaa                                       >  2:434994/1‑148 (MQ=255)
                                                                                                         gcgcgcTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACg                                     <  1:3329359/148‑1 (MQ=255)
                                                                                                         gcgcgcTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACg                                     <  1:129781/148‑1 (MQ=255)
                                                                                                               tctTACCTTTCGCTTTCAGTTCTTTATCCATCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGc                               >  2:546206/1‑148 (MQ=255)
                                                                                                               tctTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGc                               <  2:172550/148‑1 (MQ=255)
                                                                                                               tctTACCTTTCGCTTTCAGTTCTTTATACAGCTCCGGGATCTCTTACCAGGTTTTTGTCGGGTTCGGCAGCAGATCTTTTTTATAAATAAGCGATAAAGCTTCAAAAGCGATCGGGTAAGCAATCAGCTTGCCCTTGTAACGTAAGGc                               >  1:3236869/1‑148 (MQ=255)
                                                                                                               tctTACCTTTCGCTTTCAGTTCTTTATACAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATATTTGTTATAAATAAGCGATAAAGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGc                               >  1:1250440/1‑148 (MQ=255)
                                                                                                                 ttACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCAt                             >  2:908629/1‑148 (MQ=255)
                                                                                                                  tACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATc                            >  2:796092/1‑148 (MQ=255)
                                                                                                                   aCCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATcc                           <  2:1642695/148‑1 (MQ=255)
                                                                                                                      tttCGCTTTAAGTTCTATATCCAGCTCCGGGATCTCTTCCACGGTTTTTGGCGGGTTCGGCAGCAGATCTATTTTATAAAACAGAGATAAAGCTTCAAAAGCGATAGGGTAAGAAATCAGCTTGCCGTTGTAACGTACGGCATACCAg                        >  2:3238593/1‑148 (MQ=255)
                                                                                                                       ttCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAgg                       >  2:843517/1‑148 (MQ=255)
                                                                                                                        tCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGt                      <  1:3205246/148‑1 (MQ=255)
                                                                                                                        tCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGt                      <  1:1460418/148‑1 (MQ=255)
                                                                                                                            tttCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAAc                  >  1:452661/1‑148 (MQ=255)
                                                                                                                             ttCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACg                 >  1:183401/1‑148 (MQ=255)
                                                                                                                              tCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCTGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACgg                >  1:2672896/1‑148 (MQ=255)
                                                                                                                              tCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACgg                >  1:1679049/1‑148 (MQ=255)
                                                                                                                               cAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGa               >  1:2265838/1‑148 (MQ=255)
                                                                                                                               cAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGa               <  2:242030/148‑1 (MQ=255)
                                                                                                                                  ttCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATAc            <  1:2470182/148‑1 (MQ=255)
                                                                                                                                  ttCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATAc            <  2:2977892/148‑1 (MQ=255)
                                                                                                                                        aTCCATCTCCTGGATCTCTTCCCTGTTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAAAGCTTCAAGAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTg      <  2:1352187/148‑1 (MQ=255)
                                                                                                                                        aTCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTg      >  2:1785079/1‑148 (MQ=255)
                                                                                                                                         tCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGt     <  1:2448730/148‑1 (MQ=255)
                                                                                                                                         tCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGt     >  2:1064277/1‑148 (MQ=255)
                                                                                                                                          ccAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTc    >  2:2548076/1‑148 (MQ=255)
                                                                                                                                            aGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCt  <  1:908629/148‑1 (MQ=255)
                                                                                                                                            aGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCt  >  2:2006094/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCT  >  CP009273/4235726‑4236013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑