BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	317,205	G→A	100%	intergenic (+413/‑114)	rclR → / → ykgE	reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	317,205	0	G	A	99.6%	769.4 / ‑5.9	261	intergenic (+413/‑114)	rclR/ykgE	reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (120/134); minor base T (0/5); total (120/141)
Fisher's exact test for biased strand distribution p-value = 6.34e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 262 total aligned reads displayed.

ATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTA  >  CP009273/317058‑317346

     ggATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtg                                                                                                                                          <  2:271583/148‑1 (MQ=255)

     ggATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtg                                                                                                                                          <  2:2716750/148‑1 (MQ=255)

      gATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtgg                                                                                                                                         <  1:2688360/148‑1 (MQ=255)

       aTGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGggtggt                                                                                                                                        <  2:2169109/148‑1 (MQ=255)

             ccATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGTATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTg                                                                                                                                  >  1:2497261/1‑148 (MQ=255)

              cATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGc                                                                                                                                 <  1:3019116/148‑1 (MQ=255)

              cATTTTTAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGc                                                                                                                                 >  2:2307408/1‑148 (MQ=255)

                tttttAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTa                                                                                                                               >  1:859202/1‑148 (MQ=255)

                 ttttAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAg                                                                                                                              >  2:3016075/1‑148 (MQ=255)

                 ttttAAATGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAg                                                                                                                              <  2:615646/148‑1 (MQ=255)

                     aaaTGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCaa                                                                                                                          <  2:42607/148‑1 (MQ=255)

                      aaTGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAt                                                                                                                         <  1:224579/148‑1 (MQ=255)

                      aaTGGATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAt                                                                                                                         <  1:3127299/148‑1 (MQ=255)

                         ggATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaa                                                                                                                      >  2:2848740/1‑148 (MQ=255)

                         ggATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaa                                                                                                                      >  2:2693741/1‑148 (MQ=255)

                          gATTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaa                                                                                                                     >  1:2063730/1‑148 (MQ=255)

                           aTTTCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATCAAGGGTGGTATGTTGCTAGCCAATTaaaa                                                                                                                    >  2:1982751/1‑148 (MQ=255)

                             ttCTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATACAGGGTGGTATGTTGCTAGCCAATTaaaaaa                                                                                                                  >  2:2597435/1‑148 (MQ=255)

                               cTTATGCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGa                                                                                                                <  1:732739/148‑1 (MQ=255)

                                    gCCAAAAATGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGcc                                                                                                           >  1:920379/1‑148 (MQ=255)

                                       aaaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                                                        >  2:398801/1‑148 (MQ=255)

                                       aaaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                                                        <  1:1483728/148‑1 (MQ=255)

                                        aaaaTGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCatat                                                                                                       <  1:3491543/148‑1 (MQ=255)

                                        aaaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCatat                                                                                                       <  2:1457872/148‑1 (MQ=255)

                                         aaaTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtatt                                                                                                      >  1:2468686/1‑148 (MQ=255)

                                           aTGAACGTCGATTTCCCCCTAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtattta                                                                                                    >  2:1147934/1‑148 (MQ=255)

                                           aTGAACGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtattta                                                                                                    <  2:2772648/148‑1 (MQ=255)

                                              aacgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtg                                                                                                 <  2:1767173/146‑1 (MQ=255)

                                              aacgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtg                                                                                                 <  1:2848816/146‑1 (MQ=255)

                                               acgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtga                                                                                                >  2:870981/2‑148 (MQ=255)

                                               acgtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTAGGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtga                                                                                                >  2:871243/2‑148 (MQ=255)

                                                  tcgATTTCCCCATAAAATGTGAGCGATGCCGAAATAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtgatga                                                                                             <  2:3143863/148‑1 (MQ=255)

                                                   cgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTgatgat                                                                                            <  1:2848358/148‑1 (MQ=255)

                                                   cgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTgatgat                                                                                            <  1:401710/148‑1 (MQ=255)

                                                         ccccATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATcc                                                                                      >  2:1136886/1‑148 (MQ=255)

                                                              tAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCgg                                                                                 <  2:1335110/148‑1 (MQ=255)

                                                               aaaaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGt                                                                                >  1:1220369/1‑148 (MQ=255)

                                                                aaaTGTGAGCGATGCCTAAATAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg                                                                               <  1:2415515/148‑1 (MQ=255)

                                                                aaaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTTGTGCATTTGACGTTTGGATGAAATATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCATTTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg                                                                               <  2:1589024/148‑1 (MQ=255)

                                                                        gCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGaa                                                                       <  2:777882/148‑1 (MQ=255)

                                                                          gATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAACGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAAc                                                                     <  1:39260/148‑1 (MQ=255)

                                                                           aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACa                                                                    >  1:1981884/1‑148 (MQ=255)

                                                                           aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACa                                                                    <  2:2063730/148‑1 (MQ=255)

                                                                              ccGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCg                                                                 <  1:1621293/148‑1 (MQ=255)

                                                                                gaaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCggg                                                               <  2:2883382/148‑1 (MQ=255)

                                                                                gaaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCggg                                                               >  1:738002/1‑148 (MQ=255)

                                                                                   agaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCAt                                                            <  2:1438114/148‑1 (MQ=255)

                                                                                     aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATaa                                                          >  1:2924912/1‑148 (MQ=255)

                                                                                      aaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATaaa                                                         <  2:210635/148‑1 (MQ=255)

                                                                                       aTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAAt                                                        <  1:1097827/148‑1 (MQ=255)

                                                                                        tAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATg                                                       <  2:920379/148‑1 (MQ=255)

                                                                                        tAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATg                                                       <  1:665469/148‑1 (MQ=255)

                                                                                           aaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGc                                                    <  1:522062/148‑1 (MQ=255)

                                                                                              tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                 >  1:94706/1‑148 (MQ=255)

                                                                                              tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                 <  2:705437/148‑1 (MQ=255)

                                                                                              tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                 >  2:3477642/1‑148 (MQ=255)

                                                                                              tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg                                                 >  1:2782767/1‑148 (MQ=255)

                                                                                                 ttATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                              <  1:1138022/148‑1 (MQ=255)

                                                                                                 ttATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                              >  1:1146379/1‑148 (MQ=255)

                                                                                                 ttATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGGGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAg                                              <  2:2751179/148‑1 (MQ=255)

                                                                                                  tATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGt                                             >  1:1713319/1‑148 (MQ=255)

                                                                                                  tATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGt                                             >  1:1552789/1‑148 (MQ=255)

                                                                                                   aTTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTa                                            <  1:3363125/148‑1 (MQ=255)

                                                                                                    ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa                                           <  2:2924936/148‑1 (MQ=255)

                                                                                                     tGCATTTGACGTTTGTATGAAAGATTATCATTTGTCATACAAATAAAGGGTTGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTTATGATTGATCACCAGGTGTGGGGGAAACATAGGGCATAAATGGGCATGAAGTAAt                                          >  1:1375502/1‑148 (MQ=255)

                                                                                                     tGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt                                          <  1:1800451/148‑1 (MQ=255)

                                                                                                     tGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt                                          <  1:2994222/148‑1 (MQ=255)

                                                                                                        aTTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGa                                       <  2:3338023/148‑1 (MQ=255)

                                                                                                         tttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAg                                      >  1:1293952/1‑148 (MQ=255)

                                                                                                            gACGTTTGGATGAAAGATTATCTTTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAt                                   <  2:1832303/148‑1 (MQ=255)

                                                                                                            gACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg                                                      <  1:852174/129‑1 (MQ=255)

                                                                                                            gACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg                                                      >  2:852174/1‑129 (MQ=255)

                                                                                                            gACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAt                                   <  1:1037417/148‑1 (MQ=255)

                                                                                                             aCGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGGTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAtt                                  >  1:1804380/1‑148 (MQ=255)

                                                                                                             aCGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAtt                                  >  1:2397131/1‑148 (MQ=255)

                                                                                                              cGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTa                                 >  2:2411436/1‑148 (MQ=255)

                                                                                                               gTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAg                                >  1:3155593/1‑148 (MQ=255)

                                                                                                                 ttGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGtt                              >  2:103527/1‑148 (MQ=255)

                                                                                                                 ttGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGtt                              >  1:1774374/1‑148 (MQ=255)

                                                                                                                  tttaTGAAAGTTTATCATTTGTAATACAAATAAAGGGTGGTATGTTGCTAGACATTTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtg                             <  2:1689030/145‑1 (MQ=255)

                                                                                                                  tGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtg                             <  1:585903/148‑1 (MQ=255)

                                                                                                                  tGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtg                             <  2:1689974/148‑1 (MQ=255)

                                                                                                                   ggATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTCTTAGTtgt                            >  2:2906786/1‑148 (MQ=255)

                                                                                                                    gATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtgtg                           >  1:823088/1‑148 (MQ=255)

                                                                                                                     aTGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGa                          >  2:3452879/1‑148 (MQ=255)

                                                                                                                          aGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg                                        >  1:2567756/1‑129 (MQ=255)

                                                                                                                              tATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                 >  1:2788026/1‑148 (MQ=255)

                                                                                                                              tATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                 >  1:1251521/1‑148 (MQ=255)

                                                                                                                               aTCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAttt                <  2:1602884/148‑1 (MQ=255)

                                                                                                                               aTCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGGGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAttt                >  2:2389400/1‑148 (MQ=255)

                                                                                                                                tCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTc               <  2:2524271/148‑1 (MQ=255)

                                                                                                                                 cATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCt              <  2:2244685/148‑1 (MQ=255)

                                                                                                                                 cATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCt              >  2:1626357/1‑148 (MQ=255)

                                                                                                                                        cATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc       <  2:1424553/148‑1 (MQ=255)

                                                                                                                                        cATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc       <  1:2566955/148‑1 (MQ=255)

                                                                                                                                        aaTACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc       <  2:1821253/147‑1 (MQ=255)

                                                                                                                                          tACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt     <  2:3012005/148‑1 (MQ=255)

                                                                                                                                           aCAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTg    >  2:1283286/1‑148 (MQ=255)

                                                                                                                                           aCAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTg    <  2:2864328/148‑1 (MQ=255)

                                                                                                                                             aaaTAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTa  <  1:2162906/148‑1 (MQ=255)

ATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTA  >  CP009273/317058‑317346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`