Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,255,009 A→T 100% V256V (GTA→GTT)  dnaB → replicative DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,255,0090AT98.3% 786.5 / 1.8 240V256V (GTA→GTT) dnaBreplicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base T (127/109);  total (128/112)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

ACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTG  >  CP009273/4254864‑4255153
                                                                                                                                                 |                                                                                                                                                
acacCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCt                                                                                                                                                >  2:843933/1‑148 (MQ=255)
   ccGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAt                                                                                                                                             <  2:2538185/148‑1 (MQ=255)
     ggTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtct                                                                                                                                           >  1:2786076/1‑148 (MQ=255)
       ttATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttc                                                                                                                                         >  1:2355531/1‑148 (MQ=255)
       ttATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttc                                                                                                                                         <  2:2717903/148‑1 (MQ=255)
       ttATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttc                                                                                                                                         <  2:3196761/148‑1 (MQ=255)
        tATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttct                                                                                                                                        >  1:1117437/1‑148 (MQ=255)
        tATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttct                                                                                                                                        >  2:1661560/1‑148 (MQ=255)
        tATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttct                                                                                                                                        <  1:1024043/148‑1 (MQ=255)
            aCGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCt                                                                                                                                    >  2:2351103/1‑148 (MQ=255)
            aCGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCt                                                                                                                                    <  2:2178101/148‑1 (MQ=255)
            aCGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCt                                                                                                                                    >  2:1524294/1‑148 (MQ=255)
              gATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTgg                                                                                                                                  <  2:874234/148‑1 (MQ=255)
              gATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTgg                                                                                                                                  <  1:1011341/148‑1 (MQ=255)
              gATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTgg                                                                                                                                  <  1:489707/148‑1 (MQ=255)
                tctcAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGgag                                                                                                                                >  1:2686008/1‑148 (MQ=255)
                  tcAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGAt                                                                                                                              >  2:3364011/1‑148 (MQ=255)
                   caacaaAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATg                                                                                                                             >  2:960260/1‑148 (MQ=255)
                      caaAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCa                                                                                                                          >  2:3252866/1‑148 (MQ=255)
                      caaAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCa                                                                                                                          >  1:2001712/1‑148 (MQ=255)
                       aaaaaaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCAt                                                                                                                         <  1:436476/148‑1 (MQ=255)
                          aaaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAg                                                                                                                      >  2:3057262/1‑148 (MQ=255)
                                 cTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGAt                                                                                                               >  2:1567281/1‑148 (MQ=255)
                                    gCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCat                                                                                                            <  1:2960931/148‑1 (MQ=255)
                                     cTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatg                                                                                                           <  2:844302/148‑1 (MQ=255)
                                     cTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatg                                                                                                           <  2:401836/148‑1 (MQ=255)
                                        gCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatgatg                                                                                                        <  2:561611/148‑1 (MQ=255)
                                          aGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCg                                                                                                      <  1:1855210/148‑1 (MQ=255)
                                          aGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCg                                                                                                      <  2:1488508/148‑1 (MQ=255)
                                              gTCTGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGt                                                                                                     <  2:3213388/145‑1 (MQ=255)
                                              gTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGt                                                                                                     >  1:3213388/1‑145 (MQ=255)
                                              gTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtct                                                                                                  <  1:3130987/148‑1 (MQ=255)
                                              gTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtct                                                                                                  <  1:2114353/148‑1 (MQ=255)
                                               tCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtctc                                                                                                 >  1:190818/1‑148 (MQ=255)
                                               tCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtctc                                                                                                 <  2:1711209/148‑1 (MQ=255)
                                                cGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTctct                                                                                                <  1:2908249/148‑1 (MQ=255)
                                                  gATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTgg                                                                                              >  1:1140795/1‑148 (MQ=255)
                                                    tttGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGTGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCg                                                                                            >  2:1809788/1‑148 (MQ=255)
                                                     ttGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGt                                                                                           >  2:1043931/1‑148 (MQ=255)
                                                         tcatcGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCt                                                                                       >  2:1770069/1‑148 (MQ=255)
                                                         tcatcGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCt                                                                                       >  1:1989189/1‑148 (MQ=255)
                                                           atcGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGt                                                                                     <  1:662833/148‑1 (MQ=255)
                                                            tcgtcgCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTc                                                                                    >  2:1957245/1‑148 (MQ=255)
                                                            tcgtcgCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTc                                                                                    >  2:1955393/1‑148 (MQ=255)
                                                            tcgtcgCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTc                                                                                    >  1:2539026/1‑148 (MQ=255)
                                                             cgtcgCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTcg                                                                                   <  2:2128130/148‑1 (MQ=255)
                                                               tcgCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTcgcg                                                                                 >  2:2965981/1‑148 (MQ=255)
                                                                 gccgcGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTcgcgcg                                                                               <  1:818524/148‑1 (MQ=255)
                                                                  ccgcGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGt                                                                              >  2:2278208/1‑148 (MQ=255)
                                                                             tcGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTa                                                                   >  2:593087/1‑148 (MQ=255)
                                                                             tcGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTa                                                                   >  1:1658297/1‑148 (MQ=255)
                                                                                   ggTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATcc                                                             <  2:2786076/148‑1 (MQ=255)
                                                                                    gTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCg                                                            >  2:1209828/1‑148 (MQ=255)
                                                                                      aaaaCAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTa                                                          >  1:2808404/1‑148 (MQ=255)
                                                                                         acaacaTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCg                                                       <  2:455275/148‑1 (MQ=255)
                                                                                              aTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAg                                                  <  2:1424228/148‑1 (MQ=255)
                                                                                               tttGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGc                                                 >  1:1173188/1‑148 (MQ=255)
                                                                                                ttGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCt                                                >  1:1621497/1‑148 (MQ=255)
                                                                                                   cGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGa                                             <  1:3314175/148‑1 (MQ=255)
                                                                                                   cGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGa                                             <  1:598719/148‑1 (MQ=255)
                                                                                                    gATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGAt                                            <  1:461245/148‑1 (MQ=255)
                                                                                                     aTGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATg                                           <  2:379289/148‑1 (MQ=255)
                                                                                                     aTGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATg                                           <  1:2319836/148‑1 (MQ=255)
                                                                                                     aTGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATg                                           >  1:2019779/1‑148 (MQ=255)
                                                                                                      tGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGa                                          >  1:1681914/1‑148 (MQ=255)
                                                                                                      tGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGa                                          <  1:1897080/148‑1 (MQ=255)
                                                                                                        aaCCTCGTCGAAAACGCGGTGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACg                                        <  1:1809788/148‑1 (MQ=255)
                                                                                                          ccTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGaa                                      >  1:902660/1‑148 (MQ=255)
                                                                                                           cTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAg                                     >  1:558573/1‑148 (MQ=255)
                                                                                                            tcgtcgAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGa                                    >  1:265490/1‑148 (MQ=255)
                                                                                                            tcgtcgAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGa                                    >  2:334712/1‑148 (MQ=255)
                                                                                                            tcgtcgAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGa                                    >  2:334748/1‑148 (MQ=255)
                                                                                                            tcgtcgAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGa                                    >  1:1539986/1‑148 (MQ=255)
                                                                                                             cgtcgAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGAc                                   >  1:952642/1‑148 (MQ=255)
                                                                                                                  aaaaCGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGgc                              >  2:1143794/1‑148 (MQ=255)
                                                                                                                     aCGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGgcgcg                           >  1:836022/1‑148 (MQ=255)
                                                                                                                       gcggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCa                         <  2:2686008/148‑1 (MQ=255)
                                                                                                                        cggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAt                        <  1:2938284/148‑1 (MQ=255)
                                                                                                                        cggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAt                        <  2:902716/148‑1 (MQ=255)
                                                                                                                        cggcgATGTTGCAGGATAAACCGGATCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCCGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAt                        >  2:3478484/1‑148 (MQ=255)
                                                                                                                         ggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAtt                       <  2:504824/148‑1 (MQ=255)
                                                                                                                         ggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAtt                       <  1:4133/148‑1 (MQ=255)
                                                                                                                             aTGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCg                   >  1:2381273/1‑148 (MQ=255)
                                                                                                                             aTGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCg                   >  1:107492/1‑148 (MQ=255)
                                                                                                                                    aGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCAt            <  1:52985/148‑1 (MQ=255)
                                                                                                                                     ggATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATg           >  1:3101232/1‑148 (MQ=255)
                                                                                                                                     ggATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATg           >  1:163082/1‑148 (MQ=255)
                                                                                                                                      gATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATgg          <  1:1428037/148‑1 (MQ=255)
                                                                                                                                      gATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATgg          <  2:2658415/148‑1 (MQ=255)
                                                                                                                                      gATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATgg          <  2:2658381/148‑1 (MQ=255)
                                                                                                                                        tAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGt        <  1:2278208/148‑1 (MQ=255)
                                                                                                                                         aaaCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTa       >  2:54645/1‑148 (MQ=255)
                                                                                                                                          aaCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAt      <  1:1293792/148‑1 (MQ=255)
                                                                                                                                          aaCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAt      >  2:2214436/1‑148 (MQ=255)
                                                                                                                                           aCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAtt     >  2:2034521/1‑148 (MQ=255)
                                                                                                                                           aCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAtt     >  2:3383481/1‑148 (MQ=255)
                                                                                                                                           aCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAtt     >  2:2034212/1‑148 (MQ=255)
                                                                                                                                            ccGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCCTGGGTAttt    >  1:2776675/1‑148 (MQ=255)
                                                                                                                                            ccGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAttt    <  1:2881680/148‑1 (MQ=255)
                                                                                                                                            ccGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAttt    <  1:3364011/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTG  >  CP009273/4254864‑4255153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑