Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,274,926 A→C 100% I72M (ATT→ATG)  yjcH ← DUF485 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,274,9260AC100.0% 1175.2 / ‑6.7 288I72M (ATT→ATG) yjcHDUF485 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base C (155/131);  total (155/133)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 290 total aligned reads displayed.

TTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCG  >  CP009273/4274781‑4275072
                                                                                                                                                 |                                                                                                                                                  
  aGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCa                                                                                                                                                <  1:2020925/148‑1 (MQ=255)
    cTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACt                                                                                                                                              >  1:3062813/1‑148 (MQ=255)
     tGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTc                                                                                                                                             <  2:1809288/148‑1 (MQ=255)
            gggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACAcc                                                                                                                                      >  2:2713523/1‑148 (MQ=255)
            gggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACAcc                                                                                                                                      >  2:2625897/1‑148 (MQ=255)
              gAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCaa                                                                                                                                    >  2:404719/1‑148 (MQ=255)
               aGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAAt                                                                                                                                   <  2:989047/148‑1 (MQ=255)
               aGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAAt                                                                                                                                   >  2:531804/1‑148 (MQ=255)
                  gtgGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTgg                                                                                                                                <  2:1980833/148‑1 (MQ=255)
                      cggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATa                                                                                                                            <  1:793581/148‑1 (MQ=255)
                         cAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACcg                                                                                                                         <  2:3356223/148‑1 (MQ=255)
                         cAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACcg                                                                                                                         <  2:2468442/148‑1 (MQ=255)
                           aGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACcgcg                                                                                                                       >  1:1004410/1‑148 (MQ=255)
                           aGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACcgcg                                                                                                                       >  1:2846724/1‑148 (MQ=255)
                            gcgcCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCgt                                                                                                                      >  1:1660782/1‑148 (MQ=255)
                            gagcCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCAAACACCAATATGAATACCGAgt                                                                                                                      >  2:3248687/3‑148 (MQ=255)
                               ccGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGtgtg                                                                                                                   <  1:1490472/148‑1 (MQ=255)
                                cGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGa                                                                                                                  <  1:467365/148‑1 (MQ=255)
                                 gTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGAc                                                                                                                 >  1:641402/1‑148 (MQ=255)
                                  tCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACg                                                                                                                >  1:2544773/1‑148 (MQ=255)
                                   cAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGc                                                                                                               >  1:834718/1‑148 (MQ=255)
                                     gAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTg                                                                                                             <  2:320286/148‑1 (MQ=255)
                                     gAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTg                                                                                                             >  1:1319817/1‑148 (MQ=255)
                                       aCTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgt                                                                                                           >  1:2599850/1‑148 (MQ=255)
                                        ctctTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgtg                                                                                                          <  2:2262508/148‑1 (MQ=255)
                                         tctTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgtgt                                                                                                         >  2:2034788/1‑148 (MQ=255)
                                         tctTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgtgt                                                                                                         >  2:460383/1‑148 (MQ=255)
                                            tttCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCg                                                                                                      >  1:3339886/1‑148 (MQ=255)
                                             ttCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCgg                                                                                                     <  1:1714592/148‑1 (MQ=255)
                                               cATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGAt                                                                                                   <  1:137914/148‑1 (MQ=255)
                                                 tgatgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTAGGATTc                                                                                                 >  1:2778813/1‑148 (MQ=255)
                                                   atgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAg                                                                                               >  2:2858382/1‑148 (MQ=255)
                                                     gCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcg                                                                                             >  1:733755/1‑148 (MQ=255)
                                                       ttGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcggc                                                                                           >  1:1402675/1‑148 (MQ=255)
                                                             cTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGccc                                                                                     >  1:3398129/1‑148 (MQ=255)
                                                             cTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGccc                                                                                     <  1:3267773/148‑1 (MQ=255)
                                                               cATGCAGGACTTCATTATTAAGACGGTCGAATTCGCAGTTCTCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGAccag                                                                                   >  2:303144/1‑148 (MQ=255)
                                                                aTGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCccagc                                                                                  <  1:24388/148‑1 (MQ=255)
                                                                aTGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCccagc                                                                                  <  2:1729475/148‑1 (MQ=255)
                                                                  gCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCccagcca                                                                                >  2:1549799/1‑148 (MQ=255)
                                                                    aGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCcagccagc                                                                              <  2:883562/148‑1 (MQ=255)
                                                                      gACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAGTTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCg                                                                            >  2:3194189/1‑148 (MQ=255)
                                                                      gACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCg                                                                            >  2:2204439/1‑148 (MQ=255)
                                                                        cTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCggg                                                                          <  1:320076/148‑1 (MQ=255)
                                                                         ttCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGgc                                                                         >  1:28984/1‑148 (MQ=255)
                                                                             ttattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGa                                                                     >  1:1490922/1‑148 (MQ=255)
                                                                             ttattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGa                                                                     >  2:1140305/1‑148 (MQ=255)
                                                                              tattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGaa                                                                    >  2:3449921/1‑148 (MQ=255)
                                                                               attaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAg                                                                   <  1:1673822/148‑1 (MQ=255)
                                                                               attaAGACGGGCGAATTCGCCGTTCGCCCGCCAGAGGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAg                                                                   <  1:1673818/148‑1 (MQ=255)
                                                                                ttaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAgg                                                                  <  2:194420/148‑1 (MQ=255)
                                                                                ttaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAgg                                                                  <  2:1635465/148‑1 (MQ=255)
                                                                                  aaGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCg                                                                >  1:3474432/1‑148 (MQ=255)
                                                                                    gACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGGGCCCAGCCAGCCGGGCGCGAAGGCGAt                                                              <  2:2314280/148‑1 (MQ=255)
                                                                                     aCGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATc                                                             <  1:3118085/148‑1 (MQ=255)
                                                                                     aCGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATc                                                             >  1:1898155/1‑148 (MQ=255)
                                                                                       ggTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATGGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAg                                                           <  2:2253022/148‑1 (MQ=255)
                                                                                         tCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTa                                                         >  2:3416999/1‑148 (MQ=255)
                                                                                         tCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTa                                                         >  2:657146/1‑148 (MQ=255)
                                                                                         tCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGACAGCCGGGCGCGAAGGCGATCAGTa                                                         >  1:1142109/1‑148 (MQ=255)
                                                                                             aTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAAt                                                     >  1:398815/1‑148 (MQ=255)
                                                                                             aTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAAt                                                     >  2:2840737/1‑148 (MQ=255)
                                                                                             aTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAAt                                                     <  1:2649642/148‑1 (MQ=255)
                                                                                             aTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGGTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAAt                                                     >  1:1581004/1‑148 (MQ=255)
                                                                                               tCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACCAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCCGCGGCGTGCCCAGCCAGCCGGGCGCGACGGCGATCAGTCAAATaa                                                   >  2:2888391/1‑148 (MQ=255)
                                                                                                cGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATaaa                                                  <  2:898647/148‑1 (MQ=255)
                                                                                                  ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGc                                                >  2:480004/1‑148 (MQ=255)
                                                                                                  ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGc                                                >  1:2866377/1‑148 (MQ=255)
                                                                                                  ccGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGc                                                <  1:2151863/148‑1 (MQ=255)
                                                                                                   cGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGcc                                               <  2:3078196/148‑1 (MQ=255)
                                                                                                         cccgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGAtata                                         <  1:3011680/148‑1 (MQ=255)
                                                                                                          ccgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATaa                                        <  2:1395443/148‑1 (MQ=255)
                                                                                                           cgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATaaa                                       <  1:2288630/148‑1 (MQ=255)
                                                                                                             ccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGAGATAAACt                                     >  1:265229/1‑148 (MQ=255)
                                                                                                                gATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGcc                                  >  1:702611/1‑148 (MQ=255)
                                                                                                                 aTGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCa                                 >  1:3157085/1‑148 (MQ=255)
                                                                                                                   gTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGATGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTCAAAAACAGCCGATATAAACTGCCAGc                               >  2:3137396/1‑148 (MQ=255)
                                                                                                                    tAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGGAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCa                              >  2:1925192/1‑148 (MQ=255)
                                                                                                                    tAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCa                              >  2:917677/1‑148 (MQ=255)
                                                                                                                      gATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCata                            >  2:2761269/1‑148 (MQ=255)
                                                                                                                      gATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCata                            <  2:831842/148‑1 (MQ=255)
                                                                                                                       aTACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCataa                           >  2:1693704/1‑148 (MQ=255)
                                                                                                                       aTACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCataa                           <  1:2153996/148‑1 (MQ=255)
                                                                                                                         aCCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCataata                         >  1:1775952/1‑148 (MQ=255)
                                                                                                                         aCCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCataata                         >  1:1862822/1‑148 (MQ=255)
                                                                                                                          ccGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCAtaataa                        <  1:945965/148‑1 (MQ=255)
                                                                                                                           cGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGTAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATaataat                       <  1:1106680/148‑1 (MQ=255)
                                                                                                                                agaACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACa                  >  2:444754/1‑148 (MQ=255)
                                                                                                                                agaACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACa                  <  1:567662/148‑1 (MQ=255)
                                                                                                                                 gaACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAg                 >  1:2631847/1‑148 (MQ=255)
                                                                                                                                   aCAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGa               >  2:1439534/1‑148 (MQ=255)
                                                                                                                                   aCAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGa               >  2:1288216/1‑148 (MQ=255)
                                                                                                                                   aCAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGa               <  2:641402/148‑1 (MQ=255)
                                                                                                                                     aaaGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtg             <  2:1802425/148‑1 (MQ=255)
                                                                                                                                       aGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggt           >  1:1974410/1‑148 (MQ=255)
                                                                                                                                         gagaTCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggtgg         <  2:1071752/148‑1 (MQ=255)
                                                                                                                                         gagaTCACCATCACTCCAACACAAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggtgg         >  2:3106010/1‑148 (MQ=255)
                                                                                                                                          agaTCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGc        >  1:216746/1‑148 (MQ=255)
                                                                                                                                          agaTCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGc        >  1:204453/1‑148 (MQ=255)
                                                                                                                                             tCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGACGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCaaa     >  2:2583450/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCG  >  CP009273/4274781‑4275072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑