Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,297,846 C→A 100% M150I (ATG→ATT)  alsE ← allulose‑6‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,297,8460CA99.5% 701.1 / ‑4.3 213M150I (ATG→ATT) alsEallulose‑6‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (102/110);  total (103/110)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

GGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGG  >  CP009273/4297701‑4297990
                                                                                                                                                 |                                                                                                                                                
 gCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAAtt                                                                                                                                               >  2:1966893/1‑148 (MQ=255)
 gCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAAtt                                                                                                                                               >  2:2283253/1‑148 (MQ=255)
  ccGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAAttt                                                                                                                                              <  2:2690970/148‑1 (MQ=255)
       aGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGa                                                                                                                                         <  2:1077665/148‑1 (MQ=255)
             gacgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAAc                                                                                                                                   <  2:1707881/148‑1 (MQ=255)
             gacgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAAc                                                                                                                                   >  1:2118927/1‑148 (MQ=255)
               cgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACgg                                                                                                                                 <  2:344317/148‑1 (MQ=255)
               cgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACgg                                                                                                                                 <  1:1727135/148‑1 (MQ=255)
               cgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACgg                                                                                                                                 <  2:2290102/148‑1 (MQ=255)
                gaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGt                                                                                                                                >  1:2906709/1‑148 (MQ=255)
                   aaaGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGt                                                                                                                             <  2:2323562/148‑1 (MQ=255)
                    aaGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTc                                                                                                                            <  1:101598/148‑1 (MQ=255)
                    aaGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTc                                                                                                                            <  1:3347264/148‑1 (MQ=255)
                       aCATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCgg                                                                                                                         >  2:2785689/1‑148 (MQ=255)
                        cATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGc                                                                                                                        >  2:1263136/1‑148 (MQ=255)
                        cATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTGCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGc                                                                                                                        >  2:3098668/1‑148 (MQ=255)
                          tCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCATTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCaa                                                                                                                      >  2:2745984/1‑148 (MQ=255)
                             gCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGc                                                                                                                   <  1:1283964/148‑1 (MQ=255)
                              ccccTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGcc                                                                                                                  >  1:387890/1‑148 (MQ=255)
                                 cTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCggg                                                                                                               >  2:515054/1‑148 (MQ=255)
                                  tGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGa                                                                                                              <  2:1451763/148‑1 (MQ=255)
                                   gccgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGAt                                                                                                             <  2:13233/148‑1 (MQ=255)
                                   gccgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGAt                                                                                                             <  1:3079302/148‑1 (MQ=255)
                                   gccgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGAt                                                                                                             >  2:1814238/1‑148 (MQ=255)
                                     cgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCg                                                                                                           <  1:2983592/148‑1 (MQ=255)
                                       ccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGAc                                                                                                         >  2:676902/1‑148 (MQ=255)
                                       ccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGAc                                                                                                         >  2:2513179/1‑148 (MQ=255)
                                           cAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTc                                                                                                     <  2:1888437/148‑1 (MQ=255)
                                             gTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCAt                                                                                                   >  2:1775339/1‑148 (MQ=255)
                                              tttttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATg                                                                                                  >  2:3185453/1‑148 (MQ=255)
                                               ttttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGa                                                                                                 >  2:1422242/1‑148 (MQ=255)
                                                tttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGAc                                                                                                >  2:2927541/1‑148 (MQ=255)
                                                      aaGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAt                                                                                          >  1:1250843/1‑148 (MQ=255)
                                                      aaGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAt                                                                                          <  1:3309696/148‑1 (MQ=255)
                                                       aGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtt                                                                                         >  2:1579760/1‑148 (MQ=255)
                                                         ttGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtttt                                                                                       <  2:18480/148‑1 (MQ=255)
                                                         ttGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtttt                                                                                       <  2:218477/148‑1 (MQ=255)
                                                          tGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTa                                                                                      <  2:561315/148‑1 (MQ=255)
                                                            ccTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATc                                                                                    >  2:219300/1‑148 (MQ=255)
                                                              tGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCgg                                                                                  >  2:379089/1‑148 (MQ=255)
                                                               ggTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGc                                                                                 <  1:581204/148‑1 (MQ=255)
                                                               ggTTGCAGGAACCGTCCACCTCAATGTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGc                                                                                 <  2:930839/148‑1 (MQ=255)
                                                                gTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGcc                                                                                <  1:714951/148‑1 (MQ=255)
                                                                gTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGcc                                                                                <  1:742117/148‑1 (MQ=255)
                                                                 ttGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCt                                                                               <  1:515132/148‑1 (MQ=255)
                                                                  tGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCtt                                                                              <  1:564274/148‑1 (MQ=255)
                                                                   gCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCCGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTa                                                                             <  1:1363249/148‑1 (MQ=255)
                                                                   gCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTa                                                                             >  1:1689713/1‑148 (MQ=255)
                                                                    cAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCCGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTAt                                                                            <  1:1239009/148‑1 (MQ=255)
                                                                    cAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTAt                                                                            <  1:2709606/148‑1 (MQ=255)
                                                                      ggAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTGGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATgg                                                                          >  2:3085981/1‑148 (MQ=255)
                                                                         aCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGata                                                                       >  2:1794266/1‑148 (MQ=255)
                                                                              ccACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTa                                                                  >  2:2969140/1‑148 (MQ=255)
                                                                              ccACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTa                                                                  <  1:2374979/148‑1 (MQ=255)
                                                                              ccACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCATAATTTTATCGGCCTTATGGAGATAGTa                                                                  >  2:2058334/1‑148 (MQ=255)
                                                                               cACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAt                                                                 <  2:32523/148‑1 (MQ=255)
                                                                                aCCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAtt                                                                >  1:818687/1‑148 (MQ=255)
                                                                                 ccTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAttt                                                               >  1:2003708/1‑148 (MQ=255)
                                                                                 ccTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAttt                                                               >  2:2539509/1‑148 (MQ=255)
                                                                                  cTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCCGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTc                                                              <  1:2741044/148‑1 (MQ=255)
                                                                                  cTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTc                                                              <  1:155392/148‑1 (MQ=255)
                                                                                  cTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTc                                                              >  1:665441/1‑148 (MQ=255)
                                                                                   tCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCa                                                             >  1:1468784/1‑148 (MQ=255)
                                                                                       tttCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGc                                                         >  1:2972812/1‑148 (MQ=255)
                                                                                       tttCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAACTTTATAGGCCTTATGGATATAGTATTTAATGGc                                                         >  1:1222147/1‑148 (MQ=255)
                                                                                            tACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCaa                                                    >  1:974051/1‑148 (MQ=255)
                                                                                               tCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTg                                                 <  2:2109397/148‑1 (MQ=255)
                                                                                                ccAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTgg                                                <  1:208855/148‑1 (MQ=255)
                                                                                                ccAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTgg                                                <  1:1981492/148‑1 (MQ=255)
                                                                                                ccAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTgg                                                <  2:2393576/148‑1 (MQ=255)
                                                                                                   gACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGt                                             <  1:2284826/148‑1 (MQ=255)
                                                                                                   gACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGt                                             <  1:498841/148‑1 (MQ=255)
                                                                                                       ttCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTcc                                         <  1:2066029/148‑1 (MQ=255)
                                                                                                             ttCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTa                                   >  1:2553606/1‑148 (MQ=255)
                                                                                                             ttCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTa                                   >  1:3133947/1‑148 (MQ=255)
                                                                                                              tCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTaa                                  <  2:2003708/148‑1 (MQ=255)
                                                                                                                 cGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGa                               >  2:3345085/1‑148 (MQ=255)
                                                                                                                 cGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGa                               >  2:792954/1‑148 (MQ=255)
                                                                                                                 cGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGa                               <  1:3121605/148‑1 (MQ=255)
                                                                                                                  gCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGAt                              <  1:135301/148‑1 (MQ=255)
                                                                                                                   ccATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGATGTCCCGCAAAGCCGGGATCTACAGTCATGACCGTAATTTTATCGGCCTTATGGATATATTATATCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATc                             >  2:1197992/1‑148 (MQ=255)
                                                                                                                      tGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGc                          <  1:2433965/148‑1 (MQ=255)
                                                                                                                      tGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGc                          >  2:387522/1‑148 (MQ=255)
                                                                                                                      tGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGc                          >  1:2002867/1‑148 (MQ=255)
                                                                                                                       gCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGcc                         <  1:2160429/148‑1 (MQ=255)
                                                                                                                          ttCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCa                      >  1:3454673/1‑148 (MQ=255)
                                                                                                                           tCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCAc                     >  1:2498233/1‑148 (MQ=255)
                                                                                                                             aGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACtt                   <  1:32666/148‑1 (MQ=255)
                                                                                                                              gTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACttt                  >  1:79265/1‑148 (MQ=255)
                                                                                                                               ttCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtc                 <  1:476771/148‑1 (MQ=255)
                                                                                                                                 cGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcat               <  1:526204/148‑1 (MQ=255)
                                                                                                                                   gCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgt             <  2:3152226/148‑1 (MQ=255)
                                                                                                                                    cAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtc            >  1:1750877/1‑148 (MQ=255)
                                                                                                                                        tttATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGa        >  2:1399327/1‑148 (MQ=255)
                                                                                                                                         ttATCCAGAATTTCAGGAATGAACGGTTGTCCGGCTAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAc       <  1:2745984/148‑1 (MQ=255)
                                                                                                                                          tATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcg      <  2:3303609/148‑1 (MQ=255)
                                                                                                                                             ccAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcggcg   >  1:2304824/1‑148 (MQ=255)
                                                                                                                                             ccAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcggcg   >  1:1795185/1‑148 (MQ=255)
                                                                                                                                             ccAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcggcg   >  2:1825353/1‑148 (MQ=255)
                                                                                                                                              cAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcggcgg  <  1:2539074/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGG  >  CP009273/4297701‑4297990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑