Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,318,517 A→T 100% T522T (ACA→ACT)  crfC → clamp‑binding sister replication fork colocalization protein, dynamin‑related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,318,5170AT99.6% 732.4 / ‑6.0 223T522T (ACA→ACT) crfCclamp‑binding sister replication fork colocalization protein, dynamin‑related
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (107/114);  total (109/114)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 225 total aligned reads displayed.

GCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTT  >  CP009273/4318372‑4318661
                                                                                                                                                 |                                                                                                                                                
gcgAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGActct                                                                                                                                                <  1:3449534/148‑1 (MQ=255)
gcgAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGActct                                                                                                                                                >  1:1793372/1‑148 (MQ=255)
 cgAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTGGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTg                                                                                                                                               >  2:1861050/1‑148 (MQ=255)
   agaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGtt                                                                                                                                             <  2:2767195/148‑1 (MQ=255)
   agaTTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGtt                                                                                                                                             >  1:1679099/1‑148 (MQ=255)
       tAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCTGAGCCGTTAAGCGAGATACTTACCCGCTGTGAGACTCTGTTACAg                                                                                                                                         >  1:1101006/1‑148 (MQ=255)
       tAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAg                                                                                                                                         >  1:2922660/1‑148 (MQ=255)
           cATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACgg                                                                                                                                     <  1:996781/148‑1 (MQ=255)
           cATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACgg                                                                                                                                     <  1:2882537/148‑1 (MQ=255)
            aTGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGgc                                                                                                                                    >  2:3362337/1‑148 (MQ=255)
                  tCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGaa                                                                                                                              >  2:1208412/1‑148 (MQ=255)
                  tCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGaa                                                                                                                              <  2:25565/148‑1 (MQ=255)
                   cGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAac                                                                                                                             >  1:248974/1‑148 (MQ=255)
                    gAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGaccc                                                                                                                            <  2:2642345/148‑4 (MQ=255)
                     aGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAacac                                                                                                                           >  1:1008155/1‑148 (MQ=255)
                      gCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACAcc                                                                                                                          >  1:1719845/1‑148 (MQ=255)
                          gCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCa                                                                                                                      <  1:155189/148‑1 (MQ=255)
                            ccTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGt                                                                                                                    <  2:2667533/148‑1 (MQ=255)
                                 ccTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGa                                                                                                               <  2:561383/148‑1 (MQ=255)
                                 ccTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGa                                                                                                               <  2:540571/148‑1 (MQ=255)
                                     cGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACttt                                                                                                           <  2:436154/148‑1 (MQ=255)
                                     cGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACttt                                                                                                           <  2:1671308/148‑1 (MQ=255)
                                       ccAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTAc                                                                                                         >  1:2003477/1‑148 (MQ=255)
                                       ccAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTAc                                                                                                         <  2:1263408/148‑1 (MQ=255)
                                        cAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGGCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACg                                                                                                        <  2:391348/148‑1 (MQ=255)
                                         aaCCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGc                                                                                                       <  1:2791309/148‑1 (MQ=255)
                                          aCCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCt                                                                                                      >  1:3075398/1‑148 (MQ=255)
                                              cTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGt                                                                                                  <  1:799867/148‑1 (MQ=255)
                                               tttCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGtt                                                                                                 >  2:1607287/1‑148 (MQ=255)
                                                ttCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGttt                                                                                                <  1:686025/148‑1 (MQ=255)
                                                 tCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGtttt                                                                                               >  1:1746281/1‑148 (MQ=255)
                                                        cAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGc                                                                                        >  1:2242851/1‑148 (MQ=255)
                                                        cAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGc                                                                                        >  1:2023464/1‑148 (MQ=255)
                                                        cAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGc                                                                                        >  1:912377/1‑148 (MQ=255)
                                                         aaCAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCt                                                                                       <  1:3384235/148‑1 (MQ=255)
                                                          aCAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCtt                                                                                      >  1:229179/1‑148 (MQ=255)
                                                           cAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTg                                                                                     >  2:830879/1‑148 (MQ=255)
                                                            aGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGa                                                                                    <  2:2331792/148‑1 (MQ=255)
                                                             gCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGaa                                                                                   <  2:1317331/148‑1 (MQ=255)
                                                              cAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAAt                                                                                  >  2:705704/1‑148 (MQ=255)
                                                               aaGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATc                                                                                 <  2:3233893/148‑1 (MQ=255)
                                                                 gATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCa                                                                               >  2:1581099/1‑148 (MQ=255)
                                                                 gATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCa                                                                               <  1:2263565/148‑1 (MQ=255)
                                                                 gATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCa                                                                               <  1:836568/148‑1 (MQ=255)
                                                                  aTGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCAc                                                                              >  2:3158433/1‑148 (MQ=255)
                                                                    gcgcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCAccc                                                                            <  1:2730709/148‑1 (MQ=255)
                                                                     cgcTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCt                                                                           <  1:1041384/148‑1 (MQ=255)
                                                                       cTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCttt                                                                         >  2:2906597/1‑148 (MQ=255)
                                                                        tGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCtttt                                                                        <  2:1546113/148‑2 (MQ=255)
                                                                        tGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTg                                                                        >  1:2090631/1‑148 (MQ=255)
                                                                           aGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCg                                                                     <  2:1714196/148‑1 (MQ=255)
                                                                           aGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCg                                                                     <  1:2749814/148‑1 (MQ=255)
                                                                            ggTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCgg                                                                    >  1:725564/1‑148 (MQ=255)
                                                                             gTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCggg                                                                   <  1:1627546/148‑1 (MQ=255)
                                                                             catcAGTTAGCCGCCTTGTTACAGTATGATTCGGAGCCTTTAAGCTAGATTAGTACCAGATGTGATACTCTGTTACAGACTGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCATGAATCCACCCTTAGCCggg                                                                   >  2:2649416/4‑148 (MQ=255)
                                                                              tGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGgt                                                                  >  2:2256376/1‑148 (MQ=255)
                                                                               gCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGgtg                                                                 <  1:478405/148‑1 (MQ=255)
                                                                                cAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGgtgt                                                                <  2:1793372/148‑1 (MQ=255)
                                                                                 aGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGtt                                                               <  2:3438995/148‑1 (MQ=255)
                                                                                  gTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTa                                                              >  1:1461177/1‑148 (MQ=255)
                                                                                   ttAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTaa                                                             >  1:1275275/1‑148 (MQ=255)
                                                                                      gccgccTTGTTTGAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCg                                                          <  2:1101006/148‑1 (MQ=255)
                                                                                      gccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCg                                                          >  1:861254/1‑148 (MQ=255)
                                                                                      gccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCg                                                          <  2:2767042/148‑1 (MQ=255)
                                                                                       ccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGa                                                         <  1:2366661/148‑1 (MQ=255)
                                                                                       ccgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGa                                                         <  2:2289292/148‑1 (MQ=255)
                                                                                        cgccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGAt                                                        <  2:2827676/148‑1 (MQ=255)
                                                                                          ccTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGt                                                      <  2:1452557/148‑1 (MQ=255)
                                                                                           cTTGTTTCAGGATGATTCGGAGGCGTTATGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGTGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGtt                                                     <  2:2003148/148‑1 (MQ=255)
                                                                                            ttGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTa                                                    >  1:1340330/1‑148 (MQ=255)
                                                                                            ttGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTa                                                    <  2:3476111/148‑1 (MQ=255)
                                                                                            ttGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTa                                                    >  1:130531/1‑148 (MQ=255)
                                                                                             tGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTAt                                                   <  1:1227659/148‑1 (MQ=255)
                                                                                             tGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTAt                                                   >  2:1411635/1‑148 (MQ=255)
                                                                                                ttCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCg                                                >  2:2029528/1‑148 (MQ=255)
                                                                                                     gatgatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCa                                           <  2:2292434/148‑1 (MQ=255)
                                                                                                     gatgatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCa                                           <  2:835478/148‑1 (MQ=255)
                                                                                                      atgatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCAt                                          <  1:2537642/148‑1 (MQ=255)
                                                                                                      atgatTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCAt                                          <  1:2450545/148‑1 (MQ=255)
                                                                                                         atTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGa                                       <  1:2994389/148‑1 (MQ=255)
                                                                                                           tCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGc                                     >  1:3507407/1‑148 (MQ=255)
                                                                                                               aGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCAACAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaaca                                 >  1:3038073/1‑148 (MQ=255)
                                                                                                                gCCGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaacaa                                >  2:1383193/1‑148 (MQ=255)
                                                                                                                 ccGTTAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAg                               <  1:3321456/148‑1 (MQ=255)
                                                                                                                    ttAAGCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCa                            >  1:519940/1‑148 (MQ=255)
                                                                                                                      aaGCGAGATTCGTACCCGCTGTGAGACTCTGTTGCAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCaaa                          <  2:3044522/148‑1 (MQ=255)
                                                                                                                        gCGAGATTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAAt                        >  2:1231723/1‑148 (MQ=255)
                                                                                                                           agaTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGa                     >  1:1875122/1‑148 (MQ=255)
                                                                                                                            gaTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGaa                    <  1:1529481/148‑1 (MQ=255)
                                                                                                                             aTTCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAAt                   >  1:986993/1‑148 (MQ=255)
                                                                                                                              ttCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAAtt                  <  2:80223/148‑1 (MQ=255)
                                                                                                                               tCGTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTg                 <  2:1679099/148‑1 (MQ=255)
                                                                                                                                 gTACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAg               <  2:3418219/148‑1 (MQ=255)
                                                                                                                                  tACCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGc              <  1:2138466/148‑1 (MQ=255)
                                                                                                                                   aCCCGCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCg             >  1:3267511/1‑148 (MQ=255)
                                                                                                                                       gCTGTGAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTc         <  2:248974/148‑1 (MQ=255)
                                                                                                                                          gtgAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAgg      >  2:2262690/1‑148 (MQ=255)
                                                                                                                                          gtgAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAgg      >  1:962370/1‑148 (MQ=255)
                                                                                                                                           tgAGACTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAggg     <  1:1200393/148‑1 (MQ=255)
                                                                                                                                              gaCTCTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGttt  <  2:861254/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCGAGATTAAACATGAAATCGAGCTGGCCCTGACCTCCGCCAACCACTTTCTGCGTCAACAGCAAGATGCGCTGAAGGTGCAGTTAGCCGCCTTGTTTCAGGATGATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAACACCATCAGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTT  >  CP009273/4318372‑4318661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑