Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,350,223 G→A 100% A510V (GCT→GTT)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,350,2230GA99.7% 922.2 / ‑5.1 278A510V (GCT→GTT) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (143/134);  total (144/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 278 total aligned reads displayed.

TTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCA  >  CP009273/4350078‑4350367
                                                                                                                                                 |                                                                                                                                                
 tGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACaa                                                                                                                                               >  1:1371532/1‑148 (MQ=255)
 tGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACaa                                                                                                                                               >  2:1763079/1‑148 (MQ=255)
 tGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACaa                                                                                                                                               <  2:3157458/148‑1 (MQ=255)
  tcGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAg                                                                                                                                              <  2:3305874/147‑1 (MQ=255)
     tGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGaaa                                                                                                                                           >  1:2262007/1‑148 (MQ=255)
      gACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAt                                                                                                                                          <  2:1692352/148‑1 (MQ=255)
      gACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAt                                                                                                                                          >  1:3063219/1‑148 (MQ=255)
      gACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAt                                                                                                                                          >  1:2408793/1‑148 (MQ=255)
        cATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAttt                                                                                                                                        <  1:3406897/148‑1 (MQ=255)
          ttCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGt                                                                                                                                      >  1:849522/1‑148 (MQ=255)
          ttCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGt                                                                                                                                      <  1:3272764/148‑1 (MQ=255)
                    aaaTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAgg                                                                                                                            <  1:1176108/148‑1 (MQ=255)
                        ttATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTaca                                                                                                                        >  1:1604217/1‑148 (MQ=255)
                           tGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTacaaca                                                                                                                     <  2:931365/148‑1 (MQ=255)
                           tGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTacaaca                                                                                                                     >  1:2505595/1‑148 (MQ=255)
                             taataaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATa                                                                                                                   >  1:447762/1‑148 (MQ=255)
                              aataaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATaa                                                                                                                  <  2:177051/148‑1 (MQ=255)
                                 aaaaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCTACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGa                                                                                                               <  2:1214273/148‑1 (MQ=255)
                                 aaaaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGa                                                                                                               <  2:60869/148‑1 (MQ=255)
                                 aaaaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGa                                                                                                               >  2:3297434/1‑148 (MQ=255)
                                    aaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACa                                                                                                            >  2:3030846/1‑148 (MQ=255)
                                     aTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGACCATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAg                                                                                                           >  2:3280617/1‑148 (MQ=255)
                                       tATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGaa                                                                                                         >  1:799142/1‑148 (MQ=255)
                                       tATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGaa                                                                                                         >  1:1990304/1‑148 (MQ=255)
                                       tATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGaa                                                                                                         >  1:2422332/1‑148 (MQ=255)
                                         tGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt                                                                                                       <  2:1901788/148‑1 (MQ=255)
                                         tGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt                                                                                                       <  2:420587/148‑1 (MQ=255)
                                         tGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt                                                                                                       <  1:1249691/148‑1 (MQ=255)
                                         tGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt                                                                                                       <  2:125379/148‑1 (MQ=255)
                                              cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGa                                                                                                  >  1:956423/1‑148 (MQ=255)
                                              cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGa                                                                                                  >  2:873762/1‑148 (MQ=255)
                                               aaataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGaa                                                                                                 <  2:3096823/148‑1 (MQ=255)
                                                     atTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATAcc                                                                                           >  1:3200256/1‑148 (MQ=255)
                                                      ttGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCa                                                                                          <  2:828016/148‑1 (MQ=255)
                                                       tGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAt                                                                                         <  2:400980/148‑1 (MQ=255)
                                                         ccGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAttt                                                                                       <  1:579172/148‑1 (MQ=255)
                                                            cAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCa                                                                                    <  2:581891/148‑1 (MQ=255)
                                                            cAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCa                                                                                    <  1:2682720/148‑1 (MQ=255)
                                                            cAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCa                                                                                    <  2:1620042/148‑1 (MQ=255)
                                                              aCATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAAt                                                                                  <  1:151328/148‑1 (MQ=255)
                                                              aCATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAAt                                                                                  <  1:1926190/148‑1 (MQ=255)
                                                               cATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATc                                                                                 >  2:2494668/1‑148 (MQ=255)
                                                                 tattatACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCa                                                                               <  1:3505747/148‑1 (MQ=255)
                                                                 tattatACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCa                                                                               <  2:467298/148‑1 (MQ=255)
                                                                    tataCCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTa                                                                            >  1:112703/1‑148 (MQ=255)
                                                                     ataCCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTaa                                                                           <  2:2367818/148‑1 (MQ=255)
                                                                      taCCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTaaa                                                                          <  2:2295308/148‑1 (MQ=255)
                                                                       aCCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAg                                                                         <  1:2836413/148‑1 (MQ=255)
                                                                        ccAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAgg                                                                        <  2:1175002/148‑1 (MQ=255)
                                                                         cAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAggg                                                                       >  1:1057088/1‑148 (MQ=255)
                                                                             aGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGtt                                                                   >  1:1312458/1‑148 (MQ=255)
                                                                               gAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTg                                                                 >  2:556409/1‑148 (MQ=255)
                                                                               gAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTg                                                                 >  2:1874493/1‑148 (MQ=255)
                                                                                  cATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGccc                                                              <  2:2671976/148‑1 (MQ=255)
                                                                                  cATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGccc                                                              <  2:251313/148‑1 (MQ=255)
                                                                                     aCAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTg                                                           >  1:2098293/1‑148 (MQ=255)
                                                                                     aCAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTg                                                           <  2:1371538/148‑1 (MQ=255)
                                                                                       aaaaaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATAACATTTTCAATCCAGTAAAGGGTGTTTGCCCCTggg                                                         >  1:1668298/1‑148 (MQ=255)
                                                                                          aaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGt                                                      >  2:1526825/1‑148 (MQ=255)
                                                                                          aaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGt                                                      >  2:3268197/1‑148 (MQ=255)
                                                                                          aaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGt                                                      <  2:1467237/148‑1 (MQ=255)
                                                                                            cTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTaa                                                    >  1:629925/1‑148 (MQ=255)
                                                                                             tCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTaaa                                                   <  1:2975994/148‑1 (MQ=255)
                                                                                              caacaacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAAt                                                  <  2:2164767/148‑1 (MQ=255)
                                                                                               aacaacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAAtt                                                 >  2:2200986/1‑148 (MQ=255)
                                                                                               aacaacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAAtt                                                 >  1:988797/1‑148 (MQ=255)
                                                                                               aacaacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAAtt                                                 >  2:363784/1‑148 (MQ=255)
                                                                                                acaacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAGGACATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTa                                                >  2:1569099/1‑148 (MQ=255)
                                                                                                  aacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTaaa                                              >  1:3309194/1‑148 (MQ=255)
                                                                                                   acaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGACTATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAg                                             >  2:807802/1‑148 (MQ=255)
                                                                                                    caaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAgg                                            >  1:1231894/1‑148 (MQ=255)
                                                                                                    caaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAgg                                            >  1:1229916/1‑148 (MQ=255)
                                                                                                       atatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCgg                                         >  2:649384/1‑148 (MQ=255)
                                                                                                        tatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGt                                        <  1:231496/148‑1 (MQ=255)
                                                                                                         atTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTg                                       >  1:2368248/1‑148 (MQ=255)
                                                                                                           ttCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgag                                     <  2:2939420/148‑1 (MQ=255)
                                                                                                           ttCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgag                                     <  2:3466973/148‑1 (MQ=255)
                                                                                                           ttCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgag                                     <  1:2174557/148‑1 (MQ=255)
                                                                                                           ttCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgag                                     >  1:718902/1‑148 (MQ=255)
                                                                                                            tCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgaga                                    <  1:884570/148‑1 (MQ=255)
                                                                                                            tCCGAGCATAAATCAACCCGGAGTCACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgaga                                    <  2:355384/148‑1 (MQ=255)
                                                                                                              cGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGata                                  >  2:1814744/1‑148 (MQ=255)
                                                                                                              cGAGCAGAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGata                                  <  2:3370326/148‑1 (MQ=255)
                                                                                                                 gCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGc                               >  2:2884744/1‑148 (MQ=255)
                                                                                                                 gCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGc                               <  2:794765/148‑1 (MQ=255)
                                                                                                                  cATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCa                              <  2:1940857/148‑1 (MQ=255)
                                                                                                                    tAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATc                            <  2:744586/148‑1 (MQ=255)
                                                                                                                     aaaTCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCa                           >  1:648128/1‑148 (MQ=255)
                                                                                                                       aTCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgc                         >  1:2756974/1‑148 (MQ=255)
                                                                                                                       aTCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgc                         <  1:3372389/148‑1 (MQ=255)
                                                                                                                         cAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgctg                       >  2:271569/1‑148 (MQ=255)
                                                                                                                           aCCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgctgct                     >  1:2106210/1‑148 (MQ=255)
                                                                                                                            cccGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCtt                    >  1:1882633/1‑148 (MQ=255)
                                                                                                                             ccGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTc                   <  1:1872569/148‑1 (MQ=255)
                                                                                                                                   ttACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGtt             >  2:2257811/1‑148 (MQ=255)
                                                                                                                                    tACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTc            <  1:2572197/148‑1 (MQ=255)
                                                                                                                                      cTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCAt          >  2:1020615/1‑148 (MQ=255)
                                                                                                                                       ttATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATc         >  1:1787225/1‑148 (MQ=255)
                                                                                                                                       ttATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATc         >  1:3314397/1‑148 (MQ=255)
                                                                                                                                            cTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCtt    <  1:2729114/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCA  >  CP009273/4350078‑4350367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑