Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,359,686 C→A 66.0% E182* (GAA→TAA)  yjeH ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,359,6860CA66.0% 163.4 / 244.8 217E182* (GAA→TAA) yjeHputative transporter
Reads supporting (aligned to +/- strand):  ref base C (42/32);  new base A (80/63);  total (122/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

AATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATACCACAC  >  CP009273/4359541‑4359833
                                                                                                                                                 |                                                                                                                                                   
  tCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGata                                                                                                                                                 >  1:2918815/1‑148 (MQ=255)
  tCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGata                                                                                                                                                 <  2:2060622/148‑1 (MQ=255)
   ccTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGatat                                                                                                                                                >  2:926768/1‑148 (MQ=255)
   ccTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGatat                                                                                                                                                >  2:1514035/1‑148 (MQ=255)
   ccTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGatat                                                                                                                                                >  1:2651872/1‑148 (MQ=255)
       ccAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTAcc                                                                                                                                            >  2:2677253/1‑148 (MQ=255)
       ccAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTAcc                                                                                                                                            <  1:1843278/148‑1 (MQ=255)
       ccAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTAcc                                                                                                                                            <  1:2475992/148‑1 (MQ=255)
              cagACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGcc                                                                                                                                     >  2:9093/1‑148 (MQ=255)
                 aCCAATCATCAAAGCACGATGAAAATCACGCTCTGTATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGTCAAAACACCAGAACATCACTGATAACGCAGAAATGAACCCGGTAAGTTATATATTACCAGGTGCCGGa                                                                                                                                  <  2:1447148/148‑1 (MQ=255)
                 aCCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGa                                                                                                                                  >  1:2040295/1‑148 (MQ=255)
                   cAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGaaa                                                                                                                                <  2:2631531/148‑1 (MQ=255)
                    aaTCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAg                                                                                                                               <  2:2456999/148‑1 (MQ=255)
                     atcatcAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAgg                                                                                                                              <  2:1899828/148‑1 (MQ=255)
                     atcatcAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGGAAGTTAGATATTACCAGGTGCCGGAAAgg                                                                                                                              <  1:2416329/148‑1 (MQ=255)
                        atcaAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGa                                                                                                                           >  1:159097/1‑148 (MQ=255)
                         tcaAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGat                                                                                                                          >  1:3108721/1‑148 (MQ=255)
                          caAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGata                                                                                                                         <  1:1234306/148‑1 (MQ=255)
                          caAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGata                                                                                                                         >  1:121759/1‑148 (MQ=255)
                          caAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGata                                                                                                                         >  1:121430/1‑148 (MQ=255)
                            aaGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATAtt                                                                                                                       >  2:2619863/1‑148 (MQ=255)
                            aaGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATAtt                                                                                                                       >  2:2620203/1‑148 (MQ=255)
                              gCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCg                                                                                                                     >  2:1741335/1‑148 (MQ=255)
                               cACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGc                                                                                                                    <  1:1791068/148‑1 (MQ=255)
                                 cGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAg                                                                                                                  >  1:3508122/1‑148 (MQ=255)
                                   aGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGt                                                                                                                <  2:1542400/148‑1 (MQ=255)
                                    ggAAAATCACGCTCTGGATTTTTAAATTCCGAGTCGTGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAAACCGGTAAGTTAGATATTACCAGGTGCCTGAAAGGGGATATTCTCAGGtt                                                                                                               <  2:320634/148‑1 (MQ=255)
                                      aaaaTCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTg                                                                                                             <  1:694719/148‑1 (MQ=255)
                                       aaaTCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCTGGTCAGTTCGATATTACCAGGTGCCGGAACGGGGATATTCGCAGGTTTGa                                                                                                            >  2:1225219/1‑148 (MQ=255)
                                         aTCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGata                                                                                                          <  1:2994894/148‑1 (MQ=255)
                                         aTCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGata                                                                                                          <  2:821428/148‑1 (MQ=255)
                                          tCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGatat                                                                                                         <  1:359667/148‑1 (MQ=255)
                                          tCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGatat                                                                                                         >  1:2263310/1‑148 (MQ=255)
                                          tCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGatat                                                                                                         >  1:2630428/1‑148 (MQ=255)
                                                 ctGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATcgcccgc                                                                                                  <  1:109223/148‑1 (MQ=255)
                                                       ttttAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCccacca                                                                                            >  2:3018078/1‑148 (MQ=255)
                                                       ttttAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCccacca                                                                                            >  1:2970151/1‑148 (MQ=255)
                                                          tAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGAt                                                                                         >  2:3267508/1‑148 (MQ=255)
                                                          tAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGAt                                                                                         <  2:1592339/148‑1 (MQ=255)
                                                             aTTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGATTGATATCGCCCGCCCACCAGATAGc                                                                                      >  2:200896/1‑148 (MQ=255)
                                                             aTTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGc                                                                                      >  1:2554911/1‑148 (MQ=255)
                                                              ttCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCg                                                                                     >  2:701482/1‑148 (MQ=255)
                                                                 cGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACa                                                                                  <  1:2903069/148‑1 (MQ=255)
                                                                  gAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACaa                                                                                 <  2:2676544/148‑1 (MQ=255)
                                                                   aGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAAt                                                                                <  2:1441608/148‑1 (MQ=255)
                                                                     gCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCa                                                                              >  2:1724856/1‑148 (MQ=255)
                                                                      cGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAg                                                                             >  1:1034154/1‑148 (MQ=255)
                                                                      cGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAg                                                                             >  1:996390/1‑148 (MQ=255)
                                                                            gggCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGcgac                                                                       <  2:1586575/148‑1 (MQ=255)
                                                                               cAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGAt                                                                    <  2:3031194/148‑1 (MQ=255)
                                                                                 aaTGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATaa                                                                  <  1:853144/148‑1 (MQ=255)
                                                                                 aaTGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATaa                                                                  >  1:1131911/1‑148 (MQ=255)
                                                                                   tGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGt                                                                <  2:3391827/148‑1 (MQ=255)
                                                                                       tCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCgg                                                            >  1:1902184/1‑148 (MQ=255)
                                                                                       tCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCgg                                                            >  1:1608034/1‑148 (MQ=255)
                                                                                        ccAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGc                                                           >  1:1671625/1‑148 (MQ=255)
                                                                                        ccAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGc                                                           >  2:1419951/1‑148 (MQ=255)
                                                                                         cAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCa                                                          >  2:2305987/1‑148 (MQ=255)
                                                                                          aGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCaa                                                         <  1:2081663/148‑1 (MQ=255)
                                                                                            aCCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATa                                                       >  1:406320/1‑148 (MQ=255)
                                                                                                 cAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGt                                                  >  1:1688202/1‑148 (MQ=255)
                                                                                                       acCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAg                                            <  2:1073291/148‑1 (MQ=255)
                                                                                                        ccAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGa                                           >  2:790740/1‑148 (MQ=255)
                                                                                                          aGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATAGTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGAtt                                         <  2:40397/148‑1 (MQ=255)
                                                                                                           gAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTa                                        >  1:1371379/1‑148 (MQ=255)
                                                                                                           gAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTa                                        <  1:1419951/148‑1 (MQ=255)
                                                                                                            aaCATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAg                                       >  1:1014899/1‑148 (MQ=255)
                                                                                                              cATCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGAGAAGTCCGGCAATACCGGTGTGTAGATTAGCa                                     >  2:2932076/1‑148 (MQ=255)
                                                                                                               aTCACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCAc                                    <  2:1050899/148‑1 (MQ=255)
                                                                                                                 cACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTg                                  >  2:3335218/1‑148 (MQ=255)
                                                                                                                 cACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTg                                  >  1:2817669/1‑148 (MQ=255)
                                                                                                                 cACTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTg                                  >  1:2817651/1‑148 (MQ=255)
                                                                                                                   cTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGa                                <  1:499249/148‑1 (MQ=255)
                                                                                                                   cTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGa                                <  1:1052889/148‑1 (MQ=255)
                                                                                                                   cTGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGa                                <  2:114281/148‑1 (MQ=255)
                                                                                                                    tGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGaa                               <  1:2725462/148‑1 (MQ=255)
                                                                                                                    tGATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGaa                               >  1:1741460/1‑148 (MQ=255)
                                                                                                                     gATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAAc                              <  1:1958589/148‑1 (MQ=255)
                                                                                                                     gATAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAAc                              <  2:2917768/148‑1 (MQ=255)
                                                                                                                      aTAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACt                             >  2:2099063/1‑148 (MQ=255)
                                                                                                                      aTAACGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACt                             <  2:2823252/148‑1 (MQ=255)
                                                                                                                        aaCGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTgg                           >  2:30689/1‑148 (MQ=255)
                                                                                                                        aaCGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTgg                           <  1:225341/148‑1 (MQ=255)
                                                                                                                        aaCGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTgg                           >  2:1692704/1‑148 (MQ=255)
                                                                                                                         aCGCAGCAAATAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGc                          <  1:2689041/148‑1 (MQ=255)
                                                                                                                                aaaTAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGa                   >  1:2313730/1‑148 (MQ=255)
                                                                                                                                 aaTAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAg                  <  1:1997309/148‑1 (MQ=255)
                                                                                                                                 aaTAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAg                  >  1:3230507/1‑148 (MQ=255)
                                                                                                                                   tAACCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTa                >  1:1182744/1‑148 (MQ=255)
                                                                                                                                    aaCCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTAc               >  1:2831515/1‑148 (MQ=255)
                                                                                                                                     aCCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTAcc              >  2:2380751/1‑148 (MQ=255)
                                                                                                                                     aCCCGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTAcc              <  2:507523/148‑1 (MQ=255)
                                                                                                                                      cccGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCg             <  1:3469491/148‑1 (MQ=255)
                                                                                                                                        cGGTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGat           <  2:35821/148‑1 (MQ=255)
                                                                                                                                          gTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGatat         >  1:1233494/1‑148 (MQ=255)
                                                                                                                                          gTAAGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGatat         >  2:930106/1‑148 (MQ=255)
                                                                                                                                             aGTTAGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATAcc      >  1:2112389/1‑148 (MQ=255)
                                                                                                                                              gTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATACca     >  1:1886324/1‑148 (MQ=255)
                                                                                                                                                 cGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATACCacac  >  2:413782/1‑148 (MQ=255)
                                                                                                                                                 cGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATACCacac  <  1:535143/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAGGTTTGATATCGCCCGCCCACCAGATAGCGACAATCAGCGCGACGATAAGTCCGGCAATAACGGTTTGTAGATTAGCACTGGAACTGGCACCGCGAGTACCGATATACCACAC  >  CP009273/4359541‑4359833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑