Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,366,322 A→C 100% intergenic (+63/‑48) ecnA → / → ecnB entericidin A membrane lipoprotein, antidote entericidin B/entericidin B membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,366,3220AC98.1% 820.4 / ‑0.8 206intergenic (+63/‑48)ecnA/ecnBentericidin A membrane lipoprotein, antidote entericidin B/entericidin B membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base C (105/97);  total (107/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

TGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTT  >  CP009273/4366177‑4366466
                                                                                                                                                 |                                                                                                                                                
tgctCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCAt                                                                                                                                                <  2:1155771/148‑1 (MQ=255)
tgctCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCAt                                                                                                                                                <  1:3209177/148‑1 (MQ=255)
   tCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTg                                                                                                                                             <  1:2908452/148‑1 (MQ=255)
    cACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGc                                                                                                                                            >  2:2251509/1‑148 (MQ=255)
     aCGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCt                                                                                                                                           <  2:2108280/148‑1 (MQ=255)
     aCGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCt                                                                                                                                           <  2:2074247/148‑1 (MQ=255)
      cGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCta                                                                                                                                          <  1:2350132/148‑1 (MQ=255)
      cGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCta                                                                                                                                          >  2:3338802/1‑148 (MQ=255)
        gtcTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCtata                                                                                                                                        <  2:3189895/146‑1 (MQ=255)
        ggCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCtata                                                                                                                                        <  2:915571/148‑1 (MQ=255)
        ggCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCtata                                                                                                                                        >  2:918138/1‑148 (MQ=255)
           tGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGc                                                                                                                                     <  1:2547600/148‑1 (MQ=255)
            gTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCa                                                                                                                                    >  1:3223528/1‑148 (MQ=255)
            gTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCa                                                                                                                                    >  1:2964060/1‑148 (MQ=255)
              aaCACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCaaa                                                                                                                                  >  1:2219278/1‑148 (MQ=255)
               acacCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAAc                                                                                                                                 >  1:1398793/1‑148 (MQ=255)
               acacCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAAc                                                                                                                                 >  1:2047850/1‑148 (MQ=255)
               acacCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAAc                                                                                                                                 <  1:340417/148‑1 (MQ=255)
                    gCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACataaa                                                                                                                            <  2:710433/148‑1 (MQ=255)
                      tCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACataaata                                                                                                                          <  1:2093703/148‑1 (MQ=255)
                        gcgGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAAc                                                                                                                        <  2:606253/148‑1 (MQ=255)
                         cgGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACa                                                                                                                       <  1:1693558/148‑1 (MQ=255)
                           gTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACAtt                                                                                                                     >  1:888222/1‑148 (MQ=255)
                            tttCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTa                                                                                                                    <  1:1077774/148‑1 (MQ=255)
                            tttCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTa                                                                                                                    >  2:258430/1‑148 (MQ=255)
                            tttCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTa                                                                                                                    >  2:1081409/1‑148 (MQ=255)
                                    aaGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAg                                                                                                            >  2:330853/1‑148 (MQ=255)
                                    aaGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAg                                                                                                            >  1:1808199/1‑148 (MQ=255)
                                     aGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAgg                                                                                                           <  2:3128782/148‑1 (MQ=255)
                                     aGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAAAAACATTACATAAAAgg                                                                                                           >  1:2069290/1‑148 (MQ=255)
                                       aCATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGaa                                                                                                         >  1:2531908/1‑148 (MQ=255)
                                        cATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAg                                                                                                        >  1:326836/1‑148 (MQ=255)
                                        cATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAg                                                                                                        <  2:1963577/148‑1 (MQ=255)
                                           cAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACg                                                                                                     <  1:3346410/148‑1 (MQ=255)
                                           cAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACg                                                                                                     >  1:833742/1‑148 (MQ=255)
                                           cAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACg                                                                                                     <  1:2810323/148‑1 (MQ=255)
                                           cAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACg                                                                                                     >  2:2344467/1‑148 (MQ=255)
                                                   tcGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTg                                                                                             >  1:359022/1‑148 (MQ=255)
                                                       cAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaaga                                                                                         <  2:780666/148‑1 (MQ=255)
                                                       cAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTgaaga                                                                                         <  2:886049/148‑1 (MQ=255)
                                                             cATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACaa                                                                                   <  1:2928858/148‑1 (MQ=255)
                                                              aTCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAAt                                                                                  >  2:1574394/1‑148 (MQ=255)
                                                                ctctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTg                                                                                >  2:2673714/1‑148 (MQ=255)
                                                                   tcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGTAAGACGTTATGGTGAAGAAGACAATTGCAg                                                                             <  2:168552/148‑1 (MQ=255)
                                                                   tcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAg                                                                             <  1:3307590/148‑1 (MQ=255)
                                                                    cgcgCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGc                                                                            >  2:1214951/1‑148 (MQ=255)
                                                                      cgcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGa                                                                          >  1:3261270/1‑148 (MQ=255)
                                                                       gcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGAt                                                                         <  2:2219278/148‑1 (MQ=255)
                                                                       gcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGAt                                                                         <  1:781837/148‑1 (MQ=255)
                                                                       gcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGAt                                                                         <  1:782343/148‑1 (MQ=255)
                                                                               cTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctg                                                                 <  1:388705/148‑1 (MQ=255)
                                                                                 aaTTTTTCTTCTCTTCCGTAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgtt                                                               >  2:1281846/1‑148 (MQ=255)
                                                                                 aaTTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgtt                                                               >  2:968068/1‑148 (MQ=255)
                                                                                 aaTTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgtt                                                               >  1:1930255/1‑148 (MQ=255)
                                                                                 aaTTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTttctgtt                                                               >  1:541905/1‑148 (MQ=255)
                                                                                        cttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGc                                                        <  2:359022/148‑1 (MQ=255)
                                                                                        cttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGc                                                        >  1:2125289/1‑148 (MQ=255)
                                                                                         ttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCt                                                       <  2:532898/148‑1 (MQ=255)
                                                                                         ttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCt                                                       <  2:2906018/148‑1 (MQ=255)
                                                                                          tctcttcCGAAAAATCATCAGAATCCAATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTAAAGGCAAACATAAATAACATTACCTAAAAGTAATACGTTATGGTGAATAAGACAAAAGCAGCGATCTAATCTGTTCTGGTGCtt                                                      >  1:2928084/1‑148 (MQ=255)
                                                                                             cttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCa                                                   >  1:1056741/1‑148 (MQ=255)
                                                                                             cttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCa                                                   >  1:267158/1‑148 (MQ=255)
                                                                                               tcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAAc                                                 >  2:1057416/1‑148 (MQ=255)
                                                                                                  gAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGt                                              <  1:2003873/148‑1 (MQ=255)
                                                                                                  gAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGt                                              <  1:241484/148‑1 (MQ=255)
                                                                                                    aaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTAt                                            <  2:2365701/148‑1 (MQ=255)
                                                                                                    aaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTAt                                            >  1:3463582/1‑148 (MQ=255)
                                                                                                    aaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTAt                                            >  2:1444923/1‑148 (MQ=255)
                                                                                                     aaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTAtt                                           >  1:1485218/1‑148 (MQ=255)
                                                                                                     aaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTAtt                                           >  2:2445213/1‑148 (MQ=255)
                                                                                                       atcatcAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTCTGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTaa                                         >  2:3088926/1‑148 (MQ=255)
                                                                                                         catcaGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAAct                                       >  1:3123557/1‑148 (MQ=255)
                                                                                                         catcaGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAAct                                       >  1:2673715/1‑148 (MQ=255)
                                                                                                            caGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCCACAGTATTAActgcc                                    >  2:3361313/1‑148 (MQ=255)
                                                                                                            caGATTCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAActgcc                                    >  1:2576841/1‑148 (MQ=255)
                                                                                                                ttCCCATCATTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCa                                <  1:1210500/148‑1 (MQ=255)
                                                                                                                     atcatTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAAcacc                           <  1:2769179/148‑1 (MQ=255)
                                                                                                                     atcatTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAAcacc                           <  1:691560/148‑1 (MQ=255)
                                                                                                                        atTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCAcg                        >  2:340486/1‑148 (MQ=255)
                                                                                                                        atTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCAcg                        >  2:1461980/1‑148 (MQ=255)
                                                                                                                        atTTTTGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCAcg                        >  2:1786140/1‑148 (MQ=255)
                                                                                                                         tttttGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCAcgc                       >  1:664904/1‑148 (MQ=255)
                                                                                                                          ttttGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCAcgcg                      >  1:3065346/1‑148 (MQ=255)
                                                                                                                           tttGGCGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGt                     <  2:2669928/148‑1 (MQ=255)
                                                                                                                               gCGATGTTGTCGATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCg                 <  1:2710138/148‑1 (MQ=255)
                                                                                                                                cGATGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGt                >  1:471032/1‑148 (MQ=255)
                                                                                                                                  aTGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTg              >  1:343852/1‑148 (MQ=255)
                                                                                                                                  aTGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTg              <  2:465041/148‑1 (MQ=255)
                                                                                                                                  aTGTTGTCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTg              <  2:2231070/148‑1 (MQ=255)
                                                                                                                                  aTGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTg              <  1:2819869/148‑1 (MQ=255)
                                                                                                                                   tgttgtCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTgg             >  2:2714549/1‑148 (MQ=255)
                                                                                                                                   tgttgtCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTgg             >  1:1677089/1‑148 (MQ=255)
                                                                                                                                    gttgtCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGt            <  1:1627310/148‑1 (MQ=255)
                                                                                                                                    gttgtCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTACCTGCAACACCACGCGTGGCGTTGGt            <  1:994170/148‑1 (MQ=255)
                                                                                                                                        tCTATTATTCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAg        >  1:644303/1‑148 (MQ=255)
                                                                                                                                           attattCATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACa     >  1:304673/1‑148 (MQ=255)
                                                                                                                                            ttattcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAt    <  2:139746/148‑1 (MQ=255)
                                                                                                                                             tattcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGTCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAtt   <  2:2047850/148‑1 (MQ=255)
                                                                                                                                             tattcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTGATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCACCACCACGCGTGGCGTTGGTGAAGACAtt   >  2:1207093/1‑148 (MQ=255)
                                                                                                                                              attcATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAttt  >  1:932892/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTT  >  CP009273/4366177‑4366466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑