BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,375,466	G→T	5.9%	G86V (GGA→GTA)	yjeN →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,375,466	0	G	T	5.9%	581.9 / 3.8	179	G86V (GGA→GTA)	yjeN	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (106/55); new base T (4/13); total (110/69)
Fisher's exact test for biased strand distribution p-value = 1.08e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

TGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGG  >  CP009273/4375320‑4375610

tgcctgccACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGa                                                                                                                                                 <  1:1342/148‑1 (MQ=255)

tgcctgccACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGa                                                                                                                                                 <  2:3382977/148‑1 (MQ=255)

     gccACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑CAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACgg                                                                                                                                            >  1:2156936/1‑148 (MQ=255)

      ccACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGc                                                                                                                                           <  2:1343340/148‑1 (MQ=255)

      ccACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGc                                                                                                                                           <  2:3090757/148‑1 (MQ=255)

       ctcacCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCg                                                                                                                                          <  2:2101258/146‑1 (MQ=255)

         cacCCGTAAAGTGGCGATGGTGGTGGGGAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGAGATTTATTGCGCTTATCTTATCAGATGTCAGGTTTTTCTCGGGGAATTAGTGGGATACGGCGAt                                                                                                                                        >  2:3233540/1‑148 (MQ=255)

          acCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGAtt                                                                                                                                       <  1:1024280/148‑1 (MQ=255)

            ccGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTg                                                                                                                                     <  1:1344201/148‑1 (MQ=255)

               tAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGttt                                                                                                                                  >  1:806951/1‑148 (MQ=255)

               tAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGttt                                                                                                                                  <  1:2235685/148‑1 (MQ=255)

                aaaGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGACTTAGTGGGATACGGCGATTTGTTTc                                                                                                                                 >  1:1845260/1‑148 (MQ=255)

                 aaGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCg                                                                                                                                >  2:35878/1‑148 (MQ=255)

                   gTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTa                                                                                                                              >  2:506153/1‑148 (MQ=255)

                   gTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTa                                                                                                                              >  2:506174/1‑148 (MQ=255)

                   gTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTa                                                                                                                              <  2:1046090/148‑1 (MQ=255)

                   gTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTa                                                                                                                              <  1:1122746/148‑1 (MQ=255)

                       cGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATgcg                                                                                                                          <  2:402895/148‑1 (MQ=255)

                         aTGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCt                                                                                                                        <  2:263860/148‑1 (MQ=255)

                           ggtggtggGTAGCACCATTAACACCCTCCAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCtat                                                                                                                      >  2:2438076/1‑148 (MQ=255)

                           ggtggtggGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCtat                                                                                                                      <  2:2300128/148‑1 (MQ=255)

                            gtggtggGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCtata                                                                                                                     >  2:1247235/1‑148 (MQ=255)

                                 gggTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGaa                                                                                                                <  1:2390188/148‑1 (MQ=255)

                                    tAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAAtt                                                                                                             <  1:2492998/148‑1 (MQ=255)

                                      gCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTaa                                                                                                           <  1:173774/148‑1 (MQ=255)

                                       cACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCCGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAg                                                                                                          >  1:1107153/1‑148 (MQ=255)

                                         ccATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCg                                                                                                        <  2:111566/148‑1 (MQ=255)

                                           aTTAACACCCTCAATGAGCGCGAGGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGTTTTTTCGCGGGGAATTCGTGGTATACGGCGATTTGTTTCTTATGCGCTATAGCGAAATTAAGCGAc                                                                                                      <  2:2532779/148‑1 (MQ=255)

                                           aTTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGTATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGAc                                                                                                      >  1:2443131/1‑148 (MQ=255)

                                           aTTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGTATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGAc                                                                                                      >  1:2440909/1‑148 (MQ=255)

                                            ttAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGAcc                                                                                                     >  2:595864/1‑148 (MQ=255)

                                                    cTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCt                                                                                             >  1:1940913/1‑148 (MQ=255)

                                                     tCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCtt                                                                                            >  1:2813164/1‑148 (MQ=255)

                                                     aaaacgATCGCGTGGTTAAACGG‑TGGGT‑AAACGTGCCGTATTTTTTTTGCGCTTATCTTATCCGTTGTGTGTTTTTGGGGGGGTAATTTGTGGGATACGGCTTTTTGTTTGTTATGCGATTTAGCTAAATTAAGCGACCGGTTACCtt                                                                                            <  2:1716505/143‑1 (MQ=255)

                                                        acgATCGGGTTGGTAAAGGG‑TGGGT‑AAACCGGCCGTATTTTTTTTGCGCTTATATTATCCGATGTGTGTTTTTGCGGGGGTATTTTGTGGTATAGGGCTTTTTGTTTCGTTTGCGCTATAGCTAAATTAAGCGACCGGTTACCTTATg                                                                                         <  2:2730670/146‑1 (MQ=255)

                                                        aagcgcgcgAGGGTAACGGG‑TGGCG‑AACCGTGCCTGATTTTTTTGGCGCTAATCTTATCGGATGTCTGTTTTTGGGCGGGTATTTCGTGGTATCCGGCGTTTTGTTGCGTAGGCGATTTAGCTAAATTAAGCGACCGGTTACCTTATg                                                                                         <  2:2730697/143‑1 (MQ=255)

                                                           aTCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGt                                                                                      >  2:1629190/1‑148 (MQ=255)

                                                            tCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTg                                                                                     >  2:1409079/1‑148 (MQ=255)

                                                            gcgcgAT‑GTAAACGG‑GGGGGTAAACCTGCCGTATATTTATTGCGTTTATTTTATCCGAGGTCGGTTTTTGCGGGGGGAATTTGTGGGATCCGGCGATTTGTGTCTTATGCGATTTCGCCAATTAAAGCGACCGGTTACCTTATGAGTg                                                                                     <  2:1183874/147‑1 (MQ=255)

                                                                 aTGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTgcgcgc                                                                                >  1:2845065/1‑148 (MQ=255)

                                                                  tGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCa                                                                               >  2:1385903/1‑148 (MQ=255)

                                                                   ggTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCAt                                                                              >  2:260666/1‑148 (MQ=255)

                                                                   ggTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGAGTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCAt                                                                              >  1:2193819/1‑148 (MQ=255)

                                                                     tAACCGG‑GGGGG‑AAACCTGCCGTATATTTATGGCGCTTATCTTATCCGATGTGTGTTTTTTCGCGGGTAATTTGTGGGATCCGGCGATTTGTTTCGTTTGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGt                                                                            <  2:3488498/148‑1 (MQ=255)

                                                                     tAAACGGTTGGGC‑AACCCGGCCGTATATTTTTTGCTATTATTTTTAGCGATGTCTGTTTTTTCGCGGGTATTTTGTGGTATACGGCTTTTTTTTTATTTTGCGATTTCGGCAAATAAAGCGACCGGTTACCTTATGAGTGCGCGCATg                                                                             <  2:1900253/148‑1 (MQ=255)

                                                                       aaCGG‑TGGGT‑AAACCTGCCGTATATTTTTTGCGCTTATATTATCGGTTGTCTGGTTTTTCGGGGGGATTTTGTGGTATCCGGCTTTTTGTTTCTTTTGCGCTATAGCGAATTTAAGCGACCGGTTACCTTATGAGTGCGCGCATGttt                                                                          <  2:1399554/148‑1 (MQ=255)

                                                                       aaCGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGttt                                                                          >  1:1378475/1‑148 (MQ=255)

                                                                       aaCGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGttt                                                                          >  1:136195/1‑148 (MQ=255)

                                                                           g‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCt                                                                      >  2:543569/1‑148 (MQ=255)

                                                                             tGGGG‑AAACCTGCCGTATTTTTATGGCGCTTATCTTATCCGATGTCTGGTTTTTCGGGGGTATTTAGTGGGATAGGGCTATTTTTTTCGTATGCGCTATCGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCtt                                                                     <  2:1481708/148‑1 (MQ=255)

                                                                              gggg‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTa                                                                    >  2:2435558/1‑148 (MQ=255)

                                                                                gg‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTAtg                                                                  >  1:156525/1‑148 (MQ=255)

                                                                                     aCCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTAtgctgc                                                              >  1:966836/1‑148 (MQ=255)

                                                                                       cTGCCTGATTTTTTTTGCGCTTATCTTATCCTTTGTCTGTTTTTTGGGGGGTATTTTGTGTGATACGGTTTTTTGTTTGTTTGGCGATTTAGCTAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCAt                                                            <  2:3395638/148‑1 (MQ=255)

                                                                                        ggCCTGATATTTTTTGCGCTTATCTTATCCTTTGTGTTTTTTTTCGGGGGTAATTTGTGGTATACGGCTATTTTTTTCTTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATc                                                           <  2:1689981/147‑1 (MQ=255)

                                                                                         gCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCt                                                          >  1:1997770/1‑148 (MQ=255)

                                                                                            ggATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGt                                                       >  2:934519/1‑148 (MQ=255)

                                                                                            ggATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGt                                                       >  2:2678778/1‑148 (MQ=255)

                                                                                             gATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGtt                                                      >  1:1275148/1‑148 (MQ=255)

                                                                                               tatttattGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTa                                                    >  1:1464383/1‑148 (MQ=255)

                                                                                               tatttattGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTa                                                    >  2:1908854/1‑148 (MQ=255)

                                                                                               tatttattGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTa                                                    >  1:3159219/1‑148 (MQ=255)

                                                                                                atttattGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTAt                                                   >  1:191953/1‑148 (MQ=255)

                                                                                                 tttcttGCGCTTAGATTACCGGATGGCTGGTTTTTGGCGGGTATTTTGGGGTATCCGGCGATTTGTTTGGTTTGCGCTATAGCTAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTAtt                                                  <  2:2087654/148‑1 (MQ=255)

                                                                                                 tttattGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTAtt                                                  >  2:2378245/1‑148 (MQ=255)

                                                                                                  ttattGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATtg                                                 >  1:3175277/1‑148 (MQ=255)

                                                                                                  ttattGCGATTATCTTATCCGTTGGCTGTTTTTTCGCGGGTAATTAGTGGTATACGGCTTTTTGTTTCGTATGCGCTAGAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATtg                                                 <  2:3447538/148‑1 (MQ=255)

                                                                                                     ttGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTgg                                              >  2:182483/1‑148 (MQ=255)

                                                                                                      tGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGc                                             >  2:2143426/1‑148 (MQ=255)

                                                                                                        cgcTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGt                                           >  1:304348/1‑148 (MQ=255)

                                                                                                        cgcTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGt                                           >  1:2073291/1‑148 (MQ=255)

                                                                                                        cgcTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGt                                           >  1:862960/1‑148 (MQ=255)

                                                                                                        cgcTTATCTTATCCGAGGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGt                                           >  2:366821/1‑148 (MQ=255)

                                                                                                         gcTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGtt                                          >  1:411686/1‑148 (MQ=255)

                                                                                                          cttatcttatCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTc                                         >  1:2968535/1‑148 (MQ=255)

                                                                                                             atattatcCGTTGTGTGTTTTTGCGCGGTTATTTCGTGTTATAGGGCTTTTTTTTTCCTTTGCGTTATAGCAAAATAACGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCctc                                      <  1:2769190/145‑1 (MQ=255)

                                                                                                                 tatcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGa                                  >  2:2488786/1‑148 (MQ=255)

                                                                                                                 tatcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGa                                  >  1:3183216/1‑148 (MQ=255)

                                                                                                                   tcCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATa                                >  2:202279/1‑148 (MQ=255)

                                                                                                                    ccGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATaa                               >  1:478934/1‑148 (MQ=255)

                                                                                                                    ccGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATaa                               >  1:2373442/1‑148 (MQ=255)

                                                                                                                    ccGATGTCGGGTTTGTCGGGGGTATTTAGTGGGTTCGGGCTTTTTTTTTGGTTGGGGCTATCGCGAAATTACGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATaa                               <  2:3134459/148‑1 (MQ=255)

                                                                                                                     cGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAAc                              >  2:113432/1‑148 (MQ=255)

                                                                                                                       aTGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCa                            >  1:443035/1‑148 (MQ=255)

                                                                                                                       aTGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGCTTACCTTATGAGAGCGCGCATGTTTGTCTTATGCTGAATCTGGTTTATTGTGGCGTTCCTCTGGATAAcaa                            >  1:3274890/1‑146 (MQ=255)

                                                                                                                        tgtgtgTTTTTTCGGGGGGAATTCGTGGGATCCGGCGATTTGTTTCGTAGGCGCTATAGCTAATTTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCAt                           <  2:246440/148‑1 (MQ=255)

                                                                                                                        tGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCAt                           >  1:1843605/1‑148 (MQ=255)

                                                                                                                        tGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCAt                           >  1:2023764/1‑148 (MQ=255)

                                                                                                                         gTCGGGTTTTTCCGGGGGATTTAGTGGGATACGGCGATTTTTTTCGTATGCGCTATAGCGAATTAAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATc                          <  2:3227416/148‑1 (MQ=255)

                                                                                                                          tCTGTTTTTGCGCGGGTAATTAGTGGTAAACGCCTATTTTTTTCTTATGCGCTATACCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCa                         <  2:2833700/148‑1 (MQ=255)

                                                                                                                           cTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCAc                        >  1:1577855/1‑148 (MQ=255)

                                                                                                                           cTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCAc                        >  1:649016/1‑148 (MQ=255)

                                                                                                                           cTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCAc                        >  2:2593077/1‑148 (MQ=255)

                                                                                                                           cTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCAGCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCAc                        >  2:834738/1‑148 (MQ=255)

                                                                                                                             gttttttCGCGGTTATTTTGTTGTATCCGGTTTTTTGTTTGTTATGGGATATAGATAATTTCAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACtt                      <  2:1918526/146‑1 (MQ=255)

                                                                                                                                           aaTTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACaaa        >  1:3431805/1‑148 (MQ=255)

                                                                                                                                              ttgTGGGATCCGGCGTTGTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAagag     <  2:1431017/146‑1 (MQ=255)

                                                                                                                                              tAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAagag     >  1:3343167/1‑148 (MQ=255)

                                                                                                                                              tAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAagag     >  2:2111922/1‑148 (MQ=255)

                                                                                                                                              tAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAagag     >  2:2274461/1‑148 (MQ=255)

TGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGG‑TGGGG‑AAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATCACTTCCGCGCTGGACAAAGAGTGG  >  CP009273/4375320‑4375610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`