Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,381,504 T→A 100% I28I (ATT→ATA)  orn → oligoribonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,381,5040TA98.1% 686.2 / 2.1 212I28I (ATT→ATA) ornoligoribonuclease
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base A (126/82);  total (130/82)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

AACATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAA  >  CP009273/4381360‑4381647
                                                                                                                                                |                                                                                                                                               
aaCATCTATGATACACGCAATTGTGTATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGcc                                                                                                                                              <  2:2161637/148‑1 (MQ=255)
aaCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGcc                                                                                                                                              >  2:497457/1‑148 (MQ=255)
 aCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCa                                                                                                                                             >  1:1188534/1‑148 (MQ=255)
  cATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCAc                                                                                                                                            >  1:2407442/1‑148 (MQ=255)
  cATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCAc                                                                                                                                            >  2:2959063/1‑148 (MQ=255)
       aTGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTgg                                                                                                                                       >  1:3208355/1‑148 (MQ=255)
        tGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGt                                                                                                                                      >  2:2052065/1‑148 (MQ=255)
          aTACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGa                                                                                                                                    <  2:1658537/148‑1 (MQ=255)
          aTACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGa                                                                                                                                    >  2:644880/1‑148 (MQ=255)
          aTACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGa                                                                                                                                    >  2:317212/1‑148 (MQ=255)
                 cAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGc                                                                                                                             <  2:716122/148‑1 (MQ=255)
                 cAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGc                                                                                                                             <  1:1650767/148‑1 (MQ=255)
                   aTTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCa                                                                                                                           >  1:1892258/1‑148 (MQ=255)
                   aTTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCa                                                                                                                           <  1:1517308/148‑1 (MQ=255)
                   aTTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCa                                                                                                                           >  1:1368077/1‑148 (MQ=255)
                         gATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGa                                                                                                                     <  2:675174/148‑1 (MQ=255)
                           tCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAat                                                                                                                   >  1:1279724/1‑148 (MQ=255)
                            cAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTTCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAata                                                                                                                  <  2:1494875/148‑1 (MQ=255)
                             aaTATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAatat                                                                                                                 >  1:167716/1‑148 (MQ=255)
                                ataGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCt                                                                                                              >  1:606131/1‑148 (MQ=255)
                                ataGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCt                                                                                                              >  1:937257/1‑148 (MQ=255)
                                 taGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTCTTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTg                                                                                                             >  2:1995298/1‑148 (MQ=255)
                                 taGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTg                                                                                                             <  1:2621443/148‑1 (MQ=255)
                                     cACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAg                                                                                                         >  2:1082835/1‑148 (MQ=255)
                                      aCTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGa                                                                                                        >  2:3074192/1‑148 (MQ=255)
                                        tgtgAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAg                                                                                                      <  2:2678903/148‑1 (MQ=255)
                                          tgAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAggg                                                                                                    >  1:3229730/1‑148 (MQ=255)
                                           gAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAAc                                                                                                                         <  1:250734/126‑1 (MQ=255)
                                           gAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGc                                                                                                   >  2:2295678/1‑148 (MQ=255)
                                           gAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGc                                                                                                   >  2:942737/1‑148 (MQ=255)
                                           gAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGc                                                                                                   <  1:2917156/148‑1 (MQ=255)
                                           gAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGc                                                                                                   <  1:2412060/148‑1 (MQ=255)
                                           gAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGc                                                                                                   >  1:1092201/1‑148 (MQ=255)
                                              tGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCACCCTGAATATTCTGGCAGAAGGGCCGa                                                                                                >  2:1719367/1‑148 (MQ=255)
                                                 gTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCa                                                                                             >  2:2220835/1‑148 (MQ=255)
                                                  tGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCAt                                                                                            <  1:1617321/148‑1 (MQ=255)
                                                  tGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCAt                                                                                            <  2:1217296/148‑1 (MQ=255)
                                                   ggAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCAtt                                                                                           <  1:2607269/148‑1 (MQ=255)
                                                   ggAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCAtt                                                                                           >  1:1393258/1‑148 (MQ=255)
                                                      aaaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCa                                                                                        <  1:1813049/148‑1 (MQ=255)
                                                       aaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAg                                                                                       >  2:623430/1‑148 (MQ=255)
                                                        aTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGt                                                                                      >  2:746637/1‑148 (MQ=255)
                                                        aTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGt                                                                                      >  2:1184318/1‑148 (MQ=255)
                                                          aGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTac                                                                                    >  2:408860/1‑148 (MQ=255)
                                                           gCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTaca                                                                                   <  2:2989398/148‑1 (MQ=255)
                                                             aTGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACAcc                                                                                 >  2:375911/1‑148 (MQ=255)
                                                             aTGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACAcc                                                                                 >  2:3452109/1‑148 (MQ=255)
                                                                aGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGt                                                                              <  2:837878/148‑1 (MQ=255)
                                                                 gTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTc                                                                             <  1:3174643/148‑1 (MQ=255)
                                                                  tGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCt                                                                            >  1:2858234/1‑148 (MQ=255)
                                                                  tGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCt                                                                            >  1:389030/1‑148 (MQ=255)
                                                                   gCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtg                                                                           >  2:2378124/1‑148 (MQ=255)
                                                                    ccAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtga                                                                          >  1:2964529/1‑148 (MQ=255)
                                                                    ccAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCGGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtga                                                                          <  1:385770/148‑1 (MQ=255)
                                                                       aTGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTAGTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgatga                                                                       <  1:3110352/148‑1 (MQ=255)
                                                                        tGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGaa                                                                      >  1:773286/1‑148 (MQ=255)
                                                                        tGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGaa                                                                      <  1:319887/148‑1 (MQ=255)
                                                                         gAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAAc                                                                     <  2:3208355/148‑1 (MQ=255)
                                                                          aaaaCAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACa                                                                    >  1:2601931/1‑148 (MQ=255)
                                                                           aaaCAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAg                                                                   >  2:1266904/1‑148 (MQ=255)
                                                                            aacaacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGc                                                                  <  2:2387163/148‑1 (MQ=255)
                                                                            aacaacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGc                                                                  <  1:2172948/148‑1 (MQ=255)
                                                                             acaacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACACTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCt                                                                 >  1:1011085/1‑148 (MQ=255)
                                                                              caacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTg                                                                >  2:884547/1‑148 (MQ=255)
                                                                                 ccTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGgcg                                                             <  1:992619/148‑1 (MQ=255)
                                                                                  cTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGgcgc                                                            >  2:3290555/1‑148 (MQ=255)
                                                                                   tGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCt                                                           >  2:1311033/1‑148 (MQ=255)
                                                                                    gATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTg                                                          >  1:2307606/1‑148 (MQ=255)
                                                                                    gATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTg                                                          >  1:2000452/1‑148 (MQ=255)
                                                                                    gATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTg                                                          <  2:1831332/148‑1 (MQ=255)
                                                                                      tttGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgat                                                        >  2:3106418/1‑148 (MQ=255)
                                                                                      tttGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgat                                                        >  2:1324680/1‑148 (MQ=255)
                                                                                         ggATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCGGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatgga                                                     <  1:2552403/148‑1 (MQ=255)
                                                                                          gatcgatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatggat                                                    <  1:2759029/148‑1 (MQ=255)
                                                                                           atcgatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatggatg                                                   >  2:754557/1‑148 (MQ=255)
                                                                                                tcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTgg                                              >  2:252529/1‑148 (MQ=255)
                                                                                                   tGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAAc                                           >  2:2946009/1‑148 (MQ=255)
                                                                                                      gaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTg                                        >  2:2075077/1‑148 (MQ=255)
                                                                                                        tGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTgcg                                      >  1:268314/1‑148 (MQ=255)
                                                                                                            cGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCAcc                                  <  1:2125654/148‑1 (MQ=255)
                                                                                                            cGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCGGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCAcc                                  <  1:497457/148‑1 (MQ=255)
                                                                                                             ggTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCAccc                                 <  2:3395158/148‑1 (MQ=255)
                                                                                                               tCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCAt                               >  1:981420/1‑148 (MQ=255)
                                                                                                                  ggATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATAcc                            >  1:3411982/1‑148 (MQ=255)
                                                                                                                      cccGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCa                        >  1:1972084/1‑148 (MQ=255)
                                                                                                                           gcgcGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCggg                   >  1:3235466/1‑148 (MQ=255)
                                                                                                                           gcgcGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCggg                   <  1:2052065/148‑1 (MQ=255)
                                                                                                                            cgcgATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGc                  <  1:1532294/148‑1 (MQ=255)
                                                                                                                             gcgATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCt                 <  2:1393258/148‑1 (MQ=255)
                                                                                                                               gATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTgg               >  1:2215134/1‑148 (MQ=255)
                                                                                                                                aTCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGt              >  2:2787857/1‑148 (MQ=255)
                                                                                                                                aTCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGt              >  2:2451131/1‑148 (MQ=255)
                                                                                                                                 tCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTa             >  1:2811458/1‑148 (MQ=255)
                                                                                                                                 tCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTa             >  2:2378711/1‑148 (MQ=255)
                                                                                                                                    cATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTagag          <  2:2875749/148‑1 (MQ=255)
                                                                                                                                      ttattGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAgcg        >  1:122118/1‑148 (MQ=255)
                                                                                                                                      ttattGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAgcg        <  1:316907/148‑1 (MQ=255)
                                                                                                                                          tGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTg    >  1:3500699/1‑148 (MQ=255)
                                                                                                                                          tGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTg    >  1:1763588/1‑148 (MQ=255)
                                                                                                                                            agaTAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGaa  >  1:798954/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
AACATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAA  >  CP009273/4381360‑4381647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑